JPH0215005A - Sterilizing composition for nonmedical treatment - Google Patents
Sterilizing composition for nonmedical treatmentInfo
- Publication number
- JPH0215005A JPH0215005A JP16271588A JP16271588A JPH0215005A JP H0215005 A JPH0215005 A JP H0215005A JP 16271588 A JP16271588 A JP 16271588A JP 16271588 A JP16271588 A JP 16271588A JP H0215005 A JPH0215005 A JP H0215005A
- Authority
- JP
- Japan
- Prior art keywords
- salt
- compound
- composition
- agent
- salts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 27
- 230000001954 sterilising effect Effects 0.000 title claims description 10
- 150000003839 salts Chemical class 0.000 claims abstract description 19
- JFIOVJDNOJYLKP-UHFFFAOYSA-N bithionol Chemical compound OC1=C(Cl)C=C(Cl)C=C1SC1=CC(Cl)=CC(Cl)=C1O JFIOVJDNOJYLKP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229960002326 bithionol Drugs 0.000 claims abstract description 16
- 229950005344 fenticlor Drugs 0.000 claims abstract description 12
- ANUSOIHIIPAHJV-UHFFFAOYSA-N fenticlor Chemical compound OC1=CC=C(Cl)C=C1SC1=CC(Cl)=CC=C1O ANUSOIHIIPAHJV-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000004480 active ingredient Substances 0.000 claims abstract description 11
- 150000002989 phenols Chemical class 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 abstract description 11
- 150000001875 compounds Chemical class 0.000 abstract description 8
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 5
- 238000002156 mixing Methods 0.000 abstract description 5
- 239000002023 wood Substances 0.000 abstract description 5
- 239000003899 bactericide agent Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 4
- 239000003973 paint Substances 0.000 abstract description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 abstract description 3
- 235000017491 Bambusa tulda Nutrition 0.000 abstract description 3
- 241001330002 Bambuseae Species 0.000 abstract description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 abstract description 3
- 239000011425 bamboo Substances 0.000 abstract description 3
- 230000006866 deterioration Effects 0.000 abstract description 3
- 125000005843 halogen group Chemical group 0.000 abstract description 3
- 239000010985 leather Substances 0.000 abstract description 3
- 241000233866 Fungi Species 0.000 abstract description 2
- 239000000839 emulsion Substances 0.000 abstract description 2
- 239000004744 fabric Substances 0.000 abstract description 2
- 239000002917 insecticide Substances 0.000 abstract description 2
- 239000003826 tablet Substances 0.000 abstract description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000003513 alkali Substances 0.000 abstract 2
- 229910052736 halogen Inorganic materials 0.000 abstract 2
- 239000002184 metal Substances 0.000 abstract 2
- 238000009472 formulation Methods 0.000 abstract 1
- 230000036571 hydration Effects 0.000 abstract 1
- 238000006703 hydration reaction Methods 0.000 abstract 1
- 235000011837 pasties Nutrition 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 229940079593 drug Drugs 0.000 description 7
- 239000003814 drug Substances 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 6
- 230000002195 synergetic effect Effects 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000000645 desinfectant Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229910052783 alkali metal Inorganic materials 0.000 description 2
- -1 alkali metal salts Chemical class 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- DBAONWMSNZNTMN-UHFFFAOYSA-N 2-bromo-3,6-dichlorophenol Chemical compound OC1=C(Cl)C=CC(Cl)=C1Br DBAONWMSNZNTMN-UHFFFAOYSA-N 0.000 description 1
- HWWKEEKUMAZJLL-UHFFFAOYSA-N 4-bromo-2,5-dichlorophenol Chemical compound OC1=CC(Cl)=C(Br)C=C1Cl HWWKEEKUMAZJLL-UHFFFAOYSA-N 0.000 description 1
- 206010008570 Chloasma Diseases 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000002814 agar dilution Methods 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 239000003429 antifungal agent Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 239000000750 industrial fungicide Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 150000004045 organic chlorine compounds Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 150000002902 organometallic compounds Chemical class 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 230000037384 skin absorption Effects 0.000 description 1
- 231100000274 skin absorption Toxicity 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000003859 topical antiinfective agent Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は塗料、木竹製品、皮革、糊料等の産業若しくは
日常生活に必要な産業用製品又はこれらの製造工程等で
発生する有害微生物を防除するための非医療用殺菌組成
物に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to industrial products such as paints, wood and bamboo products, leather, pastes, etc. or industrial products necessary for daily life, or non-medical products for controlling harmful microorganisms generated in the manufacturing process of these products. The present invention relates to a disinfectant composition for use in disinfectants.
産業用製品又はこれらを製造する工程等に発生する微生
物を防除するための非医療用殺菌組成物としては従来よ
り、主として、有機溶剤、有機錫剤が広く使用されてい
た。しかしながら、この有機金属化合物を有効成分とす
る非医療用殺菌組成物は残留毒性及び薬剤使用時の安定
性に問題があるとされていた。これに代る薬剤として有
機塩素系化合物、第４級アンモニウム塩等が使用されて
きた。しかし、最近特に、細菌、黴類、酵母類による微
生物災害が多発しているが、これらに対してこれら従来
の薬剤では効果が充分でなく、新しい薬剤の開発が強く
要望されている。Conventionally, organic solvents and organic tin agents have been widely used as non-medical sterilizing compositions for controlling microorganisms generated in industrial products or the processes for manufacturing them. However, non-medical sterilizing compositions containing this organometallic compound as an active ingredient have been thought to have problems with residual toxicity and stability during drug use. Organic chlorine compounds, quaternary ammonium salts, and the like have been used as alternative agents. However, recently, microbial disasters caused by bacteria, molds, and yeasts have been occurring frequently, and these conventional drugs are not sufficiently effective against these, and there is a strong demand for the development of new drugs.
本発明者らはかかる要望に鑑み、多くの細菌、黴に対し
て低薬量で活性を示す非医療用殺菌剤を開発するため鋭
意研究を重ねた結果、従来単独で殺菌剤として知られ、
また単独で殺菌剤として使用した場合充分満足し得なか
った特定の化合物を組合せて使用すると、それぞれ単独
の薬効からは全く予想し得ない種々の優れた相乗的効果
を有する非医療用殺菌組成物が見出された。In view of this demand, the present inventors have conducted intensive research to develop a non-medical disinfectant that is active against many bacteria and molds at low dosages.
In addition, when specific compounds that were not fully satisfactory when used alone as bactericidal agents are used in combination, non-medical bactericidal compositions have various excellent synergistic effects that could not be expected from the medicinal effects of each of them alone. was discovered.
かくして、本発明によれば、（ａ）ビチオノール又はフ
ェンチクロル或いはそれらの塩類及び（ｂ）トリハロゲ
ン化フェノール或いはその塩類を有効成分どする非医療
用殺菌剤が提供される。Thus, according to the present invention, there is provided a non-medical disinfectant whose active ingredients are (a) bithionol or fentichlor or salts thereof and (b) trihalogenated phenol or salts thereof.
かかる本発明の組成物を使用すると、それぞれ単独で使
用する場合の効果から、全く予想し難い卓越した相乗的
な殺菌活性か得られる。When such compositions of the present invention are used, an outstanding synergistic bactericidal activity is obtained which is completely unexpected from the effect when each is used alone.
本発明における（ａ）ヒチオノール又はフェンチクロル
は下記一般式（Ｉ）で表わされる化合物である。(a) Hythionol or fentichlor in the present invention is a compound represented by the following general formula (I).
０Ｍ Ｍ（（
［但し式中、Ｍは水素原子、アルカリ金属及びアルカリ
土類金属からなる群から選ばれ、モしてＸはハロゲン原
子を示し、ｎは１又は２の整数を示す。１
上記一般式（Ｉ）においてｎが２の場合がビチオノール
であり、ｎが１の場合がフェンチクロルである。0 MM In general formula (I), when n is 2, it is bithionol, and when n is 1, it is fentichlor.
上記ビチオノールは、細菌、真菌に対して抗菌作用がる
殺菌剤として開発されたものであり黄色ブドウ球菌の発
育阻止にはｌＯ万分の１の濃度で効果があり、肺、肝、
腸のジストマや條虫駆除に内服するが化粧品や万両、シ
ャンプーに添加して常用することは、皮膚吸収により発
疹、黒革症が現われるため現在米国のＦＤＡでは化粧品
（石ケン）に添加することは禁止されているが藻類の発
生防止やゴム製品、水溶性研磨液の防腐保存料としては
現在もなお使用されている。The above-mentioned bitionol was developed as a bactericidal agent with antibacterial effects against bacteria and fungi, and is effective in inhibiting the growth of Staphylococcus aureus at a concentration of 1/1000 100,000 ml.
It is taken orally to treat intestinal dystomia and insect control, but regular use of it as an additive to cosmetics, soaps, and shampoos can cause rashes and melasma due to skin absorption, so the US FDA currently prohibits it from being added to cosmetics (soaps). Although it is prohibited, it is still used today to prevent the growth of algae and as a preservative for rubber products and water-soluble polishing fluids.
一方フェンチクロルは、外用抗感染抗カビ剤として開発
されたものである。本発明においてビチオノール及びフ
ェンチクロルは組合せて使用してもよい。On the other hand, fentichlor was developed as a topical anti-infective and anti-fungal agent. Bithionol and fentichlor may be used in combination in the present invention.
力木発明において、前記ビチオノール又はフェンチクロ
ルと組合せて使用される（ｂ）トリハロゲン化フェノー
ルは下記一般式（Ｉ［）で表わされる化合物である。In the strength wood invention, the trihalogenated phenol (b) used in combination with bithionol or fentichlor is a compound represented by the following general formula (I[).
［但し式中、Ｍ′は水素原子、アルカリ金属及びアルカ
リ土類金属からなる群から選ばれ、モしてＹはハロゲン
原子を示すが、３個のＹはそれぞれ同一もしくは相異な
っていてもよい。］
上記トリハロゲン化フェノールの具体例としては、 例
、ｔｉｆ、２． ４． ６− トリクロルフェノール（
以下”２，４．６−ＴＣＰ’″と略称することがある）
、２．４．６−１−リブロムフェノール（以下”２．４
．６−ＴＢ Ｐ”と略称することがある）、２、５−ジ
クロル−４−ブロムフェノール（以下”２．５．４−Ｄ
ＣＢ”と略称することがある）、２．５−ジクロル−６
−ブロムフェノール（以下゛２，５．６−ＤＣＢ”と略
称することがある）及びこれらの塩類が挙げられる。塩
類の例としては、ナトリウム塩、カリウム塩又はカルシ
ウム塩の如きアルカリ金属塩又はアルカリ土類金属塩を
示すことができる。これらのトリハロゲン化フェノール
又はその塩類は、水性ペイントの防黴、製紙ノスライム
・コントロールの如き産業用防黴剤、農業用土壌処理剤
、木材防腐などの殺菌防黴保存剤として使用されている
。しかしトリハロゲン化フェノール及びその塩類は、揮
発性が著しく高く、そのため予防効果が持続しないとい
う利用面の欠点があった。[However, in the formula, M' is selected from the group consisting of a hydrogen atom, an alkali metal, and an alkaline earth metal, and Y represents a halogen atom, but each of the three Y's may be the same or different. . ] Specific examples of the above-mentioned trihalogenated phenol include, example, tif, 2. 4. 6- Trichlorophenol (
(Hereinafter, it may be abbreviated as "2,4.6-TCP'")
, 2.4.6-1-ribromophenol (hereinafter referred to as “2.4
．． (sometimes abbreviated as “6-TB P”), 2,5-dichloro-4-bromophenol (hereinafter “2.5.4-D
(sometimes abbreviated as "CB"), 2,5-dichloro-6
-bromophenol (hereinafter sometimes abbreviated as "2,5.6-DCB") and salts thereof. Examples of salts include alkali metal salts such as sodium salts, potassium salts, or calcium salts; These trihalogenated phenols or their salts can be used as fungicides in water-based paints, industrial fungicides such as NoSlime Control for papermaking, agricultural soil treatment agents, and wood preservatives. Trihalogenated phenols and their salts are used as anti-mold preservatives.However, trihalogenated phenols and their salts have a drawback in that their preventive effects do not last long because of their extremely high volatility.
本発明の非医療用殺菌組成物は、前述したそれぞれの単
剤としては欠点があり実用上の問題があったものを、組
合せて組成物とすることにより、それぞれの殺菌効果を
単に相加して対象徴生物の種類が拡大したに止らず、微
生物災害の起因菌であり従来防除が困難であった各種の
黴や細菌を低薬量の使用によって防除でき、殊にそれぞ
れ単剤の使用量と比べて’／２〜１／１゜以下で防除で
きるという優れた相乗効果が得られた。The non-medical sterilizing composition of the present invention simply adds up the sterilizing effects of each of the above-mentioned single agents, which have drawbacks and practical problems, by combining them into a composition. Not only has the variety of symbolic organisms expanded, but various molds and bacteria that cause microbial disasters and were difficult to control in the past can be controlled by using low doses, especially when using a single agent. An excellent synergistic effect was obtained in that it was possible to control insects at a rate of 1/2 to 1/1 degree or less compared to that of the conventional method.
さらに本発明の組成物は、産業用製品の微生物災害起因
菌であるアスペルギウス属、トリコデルマ属の黴類に対
しても著しく低い濃度で活性を有しており経済的に安価
に且つ安全に防除できるという極めて優れた利点を有し
ている。Furthermore, the composition of the present invention has activity at extremely low concentrations against molds of the genus Aspergius and genus Trichoderma, which are bacteria that cause microbial disasters in industrial products, and can be economically and safely controlled. It has extremely excellent advantages.
本発明の組成物において（ａ）ビチオノール又はフェン
チクロル或いはそれらの塩類と（ｂ）トリハロゲン化フ
ェノール或いはその塩類とは、重量で１０ ： １−１
： ｔｏｏの割合、好ましくは５： ｌ−１： ２０
の割合で配合するのが望ましい。In the composition of the present invention, (a) bithionol or fentichlor or salts thereof and (b) trihalogenated phenol or salts thereof are 10:1-1 by weight.
: too ratio, preferably 5:l-1:20
It is desirable to mix in the ratio of .
本発明の組成物は、前記２種の組合せた有効成分を含有
していればよく、さらに−収約な殺菌・殺黴に使用され
ている担体に分散させるか或いは溶解させた形態であ・
〕てもよく、さらに固体担体に混合された形態であって
もよい。また必要に応じて、展着剤、安定剤、分散剤、
湿潤剤、浸透剤、懸濁向上剤などを加えることもできる
。組成物の剤型としては、水和剤、粉剤、錠剤、乳剤、
ペースト剤などのいずれであってもよい。The composition of the present invention only needs to contain the above-mentioned two types of active ingredients in combination, and is further dispersed or dissolved in a carrier used for compact sterilization and fungicides.
] or may be in the form of a mixture in a solid carrier. In addition, spreading agents, stabilizers, dispersants,
Wetting agents, penetrants, suspension enhancers, etc. can also be added. The dosage forms of the composition include wettable powders, powders, tablets, emulsions,
It may be any paste agent or the like.
本発明の組成物中には、他の殺菌剤、殺虫剤、劣化防止
剤を配合しても差支えない。Other bactericides, insecticides, and deterioration inhibitors may be added to the composition of the present invention.
本発明の組成物は、使用される対象物、目的、形態に応
じてそのまま直接施用するか、または適当な濃度に希釈
して使用することができる。The composition of the present invention can be directly applied as it is or diluted to an appropriate concentration depending on the object, purpose, and form to which it is used.
かくして本発明の非医療用殺菌組成物は、塗料、皮革、
木竹製品、糊料、織編物、紙製品、壁材料、プラスチッ
ク製品などに混入させるか或いは表面処理せしめること
により、有害微生物の発生を防止しまた生育を阻止する
のでこれら製品の品質劣化を防止のために有利に使用さ
れる。Thus, the non-medical sterilizing composition of the present invention can be applied to paints, leather,
By mixing it with wood and bamboo products, starch materials, woven and knitted fabrics, paper products, wall materials, plastic products, etc., or treating the surfaces thereof, it prevents the generation and growth of harmful microorganisms, thereby preventing quality deterioration of these products. advantageously used for.
以下実施例を示して本発明をさらに具体的に説明するが
、本発明はそれによって何等限定を受けるものではない
。EXAMPLES The present invention will be explained in more detail with reference to Examples below, but the present invention is not limited thereto in any way.
寒天希釈法による最低生育阻止濃度試験実験ｌ
各薬剤は、それぞれエタノールで有効成分濃度ビチオノ
ールの場合は４，８，１０．１５，２０゜２５．３０．
４０ｐｐｍ、２．４．６−ＴＣＰの場合は５，１０．２
０，３０，４０．５０．６０ｐｐｍに希釈し９ｃｍのベ
トリ皿１枚に２ｃｍ宛分注する。それに下記組成のサブ
ロー培地ｌＯｍＱ加温溶解して加え、よく薬剤希釈液と
を混合せしめ固化平板とする。Minimum inhibitory concentration test experiment using agar dilution method 1 The active ingredient concentration of each drug in ethanol was 4, 8, 10.15, 20°, 25.30° for bithionol.
40ppm, 5,10.2 for 2.4.6-TCP
Dilute to 0, 30, 40, 50, and 60 ppm and dispense 2 cm into a 9 cm veterinary dish. Sabouraud medium lOmQ having the following composition is added to the solution by heating and dissolved, and the mixture is thoroughly mixed with the drug dilution solution to form a solidified plate.
この上にＪＩＳ Ｚ−２９１１の方法により胞子液を
作り１０５ｗ／ｒｎＱ含有する。それをマイクロプレー
ターで寒天上に０．０１ｍＱづつ胞子液を接種した。２
８°Ｃ１−週間培養後、菌の生育の有無を観察し、°最
低生育阻止濃度を求めた。その結果の一部を下記表１に
示した。On top of this, a spore liquid was prepared according to the method of JIS Z-2911 and contained 105 w/rnQ. The spore liquid was inoculated onto agar using a microplate in an amount of 0.01 mQ. 2
After culturing at 8°C for 1 week, the presence or absence of bacterial growth was observed, and the minimum growth-inhibiting concentration was determined. Some of the results are shown in Table 1 below.
なおこの実験ｌからビチオノール及び２，４゜６−ＴＣ
Ｐの併用による相乗効果は上記表１から明らかであるが
、−層明瞭とするため実験ｌのデータを添付図１の（Ａ
）〜（Ｅ）に示した。図１中の（Ａ）〜（Ｅ）は、それ
ぞれ表１中に示した供試菌の欄に示したものと一致して
いる。図１中（Ａ）〜（Ｅ）において、横軸は２，４．
６−ＴＣＰの濃度（ｐｐｍ）であり、縦軸はビチオノー
ルの濃度である。図１中（Ａ）〜（Ｅ）のいずれも点線
よりも下方に最低生育阻止濃度が示されており、このこ
とは、ビチオノール及び２，４．６−ＴＣＰの併用によ
って相乗効果があることを明らかに示している。Furthermore, from this experiment 1, bithionol and 2,4゜6-TC
The synergistic effect of the combined use of P is clear from Table 1 above, but in order to clarify the - layer, the data of Experiment 1 is shown in attached Figure 1 (A
) to (E). (A) to (E) in FIG. 1 correspond to those shown in the column of test bacteria shown in Table 1, respectively. In (A) to (E) in FIG. 1, the horizontal axis represents 2, 4.
The concentration of 6-TCP (ppm) is shown, and the vertical axis is the concentration of bithionol. In all of (A) to (E) in Figure 1, the lowest growth inhibitory concentration is shown below the dotted line, which indicates that the combination of bitionol and 2,4.6-TCP has a synergistic effect. clearly shows.
実験２
実験方法は、前記実験１と同様に行った。但し、ビチオ
ノールの代りにビチオノール及びフェンチクロルをｌ：
ｌの配合率で混合して使用し、有効成分濃度で１，２，
５．ｔｏ、２０，５０，１００．２００，４００ｐｐｍ
で実験を行った。また２、４．６−ＴＣＰについても同
様の濃度に希釈したものを使用した。Experiment 2 The experimental method was the same as in Experiment 1 above. However, bithionol and fentichlor are used instead of bithionol:
It is used by mixing at a blending ratio of 1, 1, 2,
5. to, 20, 50, 100. 200, 400 ppm
I conducted an experiment. 2,4.6-TCP was also diluted to the same concentration and used.
その結果の一部を下記衣２に示した。A part of the results are shown in Figure 2 below.
また実験２における結果を添付図２の（Ａ）〜（Ｆ）に
示した。図２の（Ａ）〜（Ｆ）は表２中の供試菌の（Ａ
）〜（Ｆ）にそれぞれ対応している。いずれも相乗効果
を有していることを示している。Further, the results of Experiment 2 are shown in attached FIGS. 2 (A) to (F). (A) to (F) of Figure 2 are (A) of the test bacteria in Table 2.
) to (F), respectively. This shows that both have a synergistic effect.
実験３
各薬剤はそれぞれエタノールで有効成分濃度ビチオノー
ル＋フェンチクロル（１： ｌ）の混合比で１．２．５
．１０．２０．５０．１００．２００．４００ｐｐｍ、
２，４．６−ＴＣＰも同じ濃度に希釈し、９ｃｍのベト
リ皿１枚に２ｃｍ宛分注する。Experiment 3 Each drug was mixed with ethanol at an active ingredient concentration of bithionol + fentichlor (1: l) at a mixing ratio of 1.2.5.
．． 10.20.50.100.200.400ppm,
2,4.6-TCP is also diluted to the same concentration and dispensed into 2 cm portions in a 9 cm veterinary dish.
それに下記標準培地１０ｍ１２加温溶解して加え、よく
薬剤希釈液とを混合せしめ固化平板とする。Add 10 ml of the standard medium shown below after heating and dissolving it, and mix well with the drug dilution solution to form a solidified plate.
この上に、全培養で１０１０個／ｍＱを含有する細菌を
、マイクロプレーターで寒天上に０．ＯｌｍＱづつ菌を
接種した。On top of this, bacteria containing 1010 cells/mQ in total culture were plated on agar in a microplate. The bacteria were inoculated with OlmQ.
３７°Ｃ１４８時間培養後菌の生育の有無を観察し、最
低生育阻止濃度を求めた。After culturing at 37°C for 148 hours, the presence or absence of bacterial growth was observed, and the minimum growth-inhibiting concentration was determined.
上記実験の結果を下記表３に示した。The results of the above experiment are shown in Table 3 below.
その結果から菌に対しても本発明の効果が認められるこ
とが理解出来る。From the results, it can be understood that the effect of the present invention is also recognized against bacteria.
第１−（Ａ）図〜第１−（Ｅ）図及び第２（Ａ）図〜第
２−（Ｆ）図はいずれも本発明の実験ｌ及び２において
得られた薬剤の組成と最低生育阻止濃度の関係を示した
ものである。
茅
−Ｅ図
隼
−Ａ図
第１−Ｃ図
第
−Ｂ図
第
−り図
第２−Ａ図
第２−Ｃ図
第２−８図
薯２−Ｄ図
第２−Ｅ図
第２−Ｆ図Figures 1-(A) to 1-(E) and 2(A) to 2-(F) show the composition and minimum growth of the drugs obtained in Experiments 1 and 2 of the present invention. This shows the relationship between inhibitory concentrations. Kaya-E figure Hayabusa-A figure 1-C figure -B figure -ri figure 2-A figure 2-C figure 2-8 figure 2-D figure 2-E figure 2-F figure
Claims (1)
らの塩類、及び（ｂ）トリハロゲン化フェノール或いは
その塩類を有効成分として含有することを特徴とする非
医療用殺菌組成物。 ２）該有効成分が（ａ）ビチオノール又はその塩類、及
び（ｂ）トリハロゲン化フェノール又はその塩類である
特許請求の範囲第１項記載の非医療用殺菌組成物。 ３）該有効成分が（ａ）フェンチクロル又はその塩類、
及び（ｂ）トリハロゲン化フェノール又はその塩類であ
る特許請求の範囲第１項記載の非医療用殺菌組成物。[Scope of Claims] 1) A non-medical sterilizing composition characterized by containing (a) bithionol or fentichlor or salts thereof, and (b) trihalogenated phenol or salts thereof as active ingredients. 2) The non-medical sterilizing composition according to claim 1, wherein the active ingredients are (a) bithionol or its salts, and (b) trihalogenated phenol or its salts. 3) The active ingredient is (a) fentichlor or its salts,
and (b) the non-medical sterilizing composition according to claim 1, which is a trihalogenated phenol or a salt thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16271588A JP2592101B2 (en) | 1988-07-01 | 1988-07-01 | Non-medical bactericidal composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16271588A JP2592101B2 (en) | 1988-07-01 | 1988-07-01 | Non-medical bactericidal composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0215005A true JPH0215005A (en) | 1990-01-18 |
| JP2592101B2 JP2592101B2 (en) | 1997-03-19 |
Family
ID=15759918
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16271588A Expired - Fee Related JP2592101B2 (en) | 1988-07-01 | 1988-07-01 | Non-medical bactericidal composition |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2592101B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2537511A (en) * | 2013-12-10 | 2016-10-19 | Dyson Technology Ltd | A hand held appliance |
| US10004313B2 (en) | 2013-12-10 | 2018-06-26 | Dyson Technology Limited | Hand held appliance |
| US10165843B2 (en) | 2013-12-10 | 2019-01-01 | Dyson Technology Limited | Hand held appliance |
| US10441049B2 (en) | 2014-12-16 | 2019-10-15 | Dyson Technology Limited | Hand held appliance |
-
1988
- 1988-07-01 JP JP16271588A patent/JP2592101B2/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2537511A (en) * | 2013-12-10 | 2016-10-19 | Dyson Technology Ltd | A hand held appliance |
| GB2537511B (en) * | 2013-12-10 | 2017-07-19 | Dyson Technology Ltd | A hand held appliance |
| US10004313B2 (en) | 2013-12-10 | 2018-06-26 | Dyson Technology Limited | Hand held appliance |
| US10165843B2 (en) | 2013-12-10 | 2019-01-01 | Dyson Technology Limited | Hand held appliance |
| US10441049B2 (en) | 2014-12-16 | 2019-10-15 | Dyson Technology Limited | Hand held appliance |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2592101B2 (en) | 1997-03-19 |
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