JP2586998B2 - Hirata Blue Crab Attractant - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[Industrial applications]

[0002] The present invention relates to an Anomala octos (scientific name: Anomala octopies) belonging to the Coleoptera, Scarabaeidae
cotta).

[0003]

2. Description of the Related Art

[0004] Hirata blue moth, which belongs to the family Scarabaeidae, has recently emerged in southern Japan and the Kanto region, especially in Chiba and Ibaraki prefectures, and has caused serious damage to turfgrass and the like in golf courses.

[0005] It is very difficult to prevent or control the damage caused by this moss, using conventional insecticides.

In general, in order to effectively control pests, an attractant is added to a forecasting means for accurately knowing the occurrence or a new type of controlling means different from the conventional controlling means using insecticides. Have been used,

Conventionally, as an attractant for scarab beetles, LIME (L-isoleucine methyl este) which is a main component of sex pheromone of Holotrichia parallera has been used.
r) is known.

However, the attractant by LIME is
It could not be said that it had a sufficient attracting effect on Hirata blue foxglove.

Therefore, there has been a demand for an attractant having a sufficient attracting effect on Hirata moth.

[0010] The chemical structure of the sex pheromone of Pseudomonas arborealis has already been described by Real et al. As (R, Z) -5-
(-)-(Oct-1-enyl) oxacyclopentan-2-one and (R, Z) -5-(-)-(dec-
1-enyl) oxacyclopentan-2-one (Annual Meeting of the Inte
rnational Society of Chemical Ecology, Clearwater
July 31- Aug. 4,93).

[0011] However, it is desired to develop a method that is more effective in developing a method for investigating an effective generation and fate of Hirata bluegrass and capturing a large amount of adults than using the sex pheromone of Hirata bluegrass alone. Was rare.

[0012] Therefore, there has been a demand for an attractant having a stronger attracting effect on Hirata moth than in the case of using the sex pheromone alone.

[0013]

[Means for Solving the Problems]

Under these circumstances, the present inventors have
In search of an effective attractant for Hirata blue moth, we conducted intensive research.

[0015] At the golf course, Hirata Aokogane is particularly affected by Dandelion (DANDELION, Taraxacum officina).
le), the extract of the dandelion flower or the seven compounds that are the main components of the extract have a strong attracting effect not only on female but also male adults of Hirata moss. The present inventors have found that they exhibit a behavior stimulating action and that they show a strong synergistic effect when these substances are used in combination with a sex pheromone, thereby completing the present invention.

Among substances generally used as attractants for scarab beetles, particularly, anethole,
Geraniol and phenethyl propionate show a strong attraction effect and behavioral stimulating effect not only on female but also adult males of Hirata blue moss, and a strong synergistic effect by using these substances in combination with sex pheromone Have been found, and the present invention has been completed.

That is, means for solving the problems of the present invention are as follows.

First, an attractant for Hirata moth, containing one or a mixture of two or more of anethole, geraniol, and phenethyl propionate.

Secondly, an attractant for Pleurotus terrestris, containing an extract of dandelion flowers.

Third, cis-3-hexenyl acetate, benzaldehyde, phenylacetaldehyde,
An attractant for Hirata moth, containing one or a mixture of two or more of benzyl alcohol, phenethyl alcohol, phenyl acetonitrile, and benzyl benzoate.

Fourthly, an attractant for Hirata moth, comprising one or a mixture of two or more of anethole, geraniol, and phenethyl propionate, and a sex attractant component of Hirata moth.

Fifthly, an attractant for Hirata moth, comprising a flower extract of Dandelion dandelion and a sex attraction component of Hirata moth.

Sixth, anethole, geraniol, phenethyl propionate, (R, Z) -5-(-)-(oct-1-enyl) oxacyclopentan-2-one
[(R, Z) -5-(-)-(oct-1-enyl)
oxacyclopentan-2-one], (R,
Z) -5-(-)-(Deck-1-enyl) oxacyclopentan-2-one [(R, Z) -5-(-)-(d
ec-1-enyl) oxacyclopentan-
2-one].

Seventh, cis-3-hexenyl acetate, benzaldehyde, phenylacetaldehyde,
Benzyl alcohol, phenethyl alcohol, phenyl acetonitrile, benzyl benzoate, (R, Z)
-5-(-)-(oct-1-enyl) oxacyclopentan-2-one, containing (R, Z) -5-(-)-(dec-1-enyl) oxacyclopentan-2-one Hirata blue foxglove attractant.

Further, other means for solving the problem of the present invention are as follows.

Anethole, geraniol and phenethyl propionate were used at 8.5 to 9.5: 0.1 to 1.0:
An attractant for Hirata blue moth, containing a mixture of 0.1 to 1.0.

An attractant for Hirata moth, comprising a mixture of anethole, geraniol, and phenethyl propionate in a ratio of 9.0: 0.5: 0.5.

An attractant for Hirata moss, containing the major components of the flowers of Dandelion.

Cis-3-hexenyl acetate, benzaldehyde, phenylacetaldehyde, benzyl alcohol, phenethyl alcohol, phenylacetonitrile, benzyl benzoate are used in an amount of 3-5: 7-9: 1.
An attractant for Hirata moth, containing a mixture mixed at a ratio of 3 to 15: 2 to 4: 4 to 6:18 to 20:10 to 12.

Cis-3-hexenyl acetate, benzaldehyde, phenyl acetaldehyde, benzyl alcohol, phenethyl alcohol, phenyl acetonitrile, benzyl benzoate were added to 4: 8: 14: 3:
An attractant for Hirata moth, containing a mixture of 5:19:11.

An attractant for Hirata moth, comprising a major component of a flower of Dandelion and a sex-inducing component of Hirata moth.

Anethole, geraniol and phenethyl propionate were used at 8.5 to 9.5: 0.1 to 1.0:
0.1-1.0 ratio, desirably 9.0: 0.5:
0.5 and a mixture of (R, Z) -5
(-)-(Oct-1-enyl) oxacyclopentan-2-one, (R, Z) -5-(-)-(dec-1-
Enyl) oxacyclopentan-2-one by 7-9:
And a mixture mixed in a ratio of 1 to 3, preferably 8: 2.

Cis-3-hexenyl acetate, benzaldehyde, phenylacetaldehyde, benzyl alcohol, phenethyl alcohol, phenylacetonitrile, benzylbenzoate are used in an amount of 3-5: 7-9: 1.
A ratio of 3 to 15: 2 to 4: 4 to 6:18 to 20:10 to 12, preferably 4: 8: 14: 3: 5: 19: 11.
And (R, Z) -5-(-)-
(Oct-1-enyl) oxacyclopentan-2-one, (R, Z) -5-(-)-(dec-1-enyl)
An attractant for Hirata moth, comprising a mixture of oxacyclopentan-2-one in a ratio of 7 to 9: 1 to 3, preferably 8: 2.

The attractant according to the present invention comprises one or a mixture of two or more of anethole, geraniol and phenethylpropionate, which are active ingredient substances, an extract of dandelion flower or one or more of its main components. Using a mixture of two or more, the preparation can be carried out by using the formulation technique applied in preparing the sex attractant.

For example, a mixture of anethole, geraniol and phenethyl propionate may be used as it is, or may be dissolved in an organic solvent such as dichloromethane to form a solution. A practical form of attractant can be obtained by using a plastic capsule or an appropriate material or device according to the capillary mode.

The use of the attractant according to the present invention enables effective control of Pleurotus radishes, which was conventionally difficult only by using ordinary insecticides. The attraction effect becomes stronger.

The following describes the identification of the main component of the dandelion flower among the attractants according to the present invention, and the mixture of chemical substances in the same ratio as the main component and the mixture of anethole, geraniol and phenethyl propionate. The confirmation test for attracting activity is described in detail.

[0038]

Embodiment 1

(1) Identification of component substances

The identification of the component substances was carried out by a method of analyzing an n-hexane extract of dandelion flowers by GC-MS.

When an n-hexane extract of dandelion flowers collected at a golf course in Ibaraki Prefecture was injected into a gas chromatograph direct mass spectrometer (GC-MS analysis), cis-3-hexenyl acetate (4), benzaldehyde (8), phenylacetaldehyde (14), benzyl alcohol (3), phenethyl alcohol (5),
Phenyl acetonitrile (19) and benzyl benzoate (11) were identified as the major components at the ratios in parentheses.

(2) Synthesis of attractant by dandelion

The attractant 1 according to the present invention was synthesized by synthesizing a compound of a chemical substance blended in the same ratio as the main component of the dandelion flower identified by GC-MS analysis.
(A synthetic kairomone of dandelion) was obtained.

[0044]

Embodiment 2

By mixing anethole, geraniol, and phenethyl propionate at a ratio of 9.0: 0.5: 0.5, an attractant 2 according to the present invention (by a food type lure) was obtained.

[0046]

Embodiment 3

(R, Z) -5-(-)-(Oct-1-
(Enyl) oxacyclopentan-2-one, (R, Z)
By mixing -5-(-)-(dec-1-enyl) oxacyclopentan-2-one in a ratio of 8: 2,
A sex-inducing component of Hirata-mog-kogane was synthesized.

The sex-inducing component of Hirata moss and cis-3-hexenyl acetate, benzaldehyde, phenylacetaldehyde, benzyl alcohol, phenethyl alcohol, phenylacetonitrile, benzylbenzoate were mixed with 4: 8: 14: 3: 5. :
The attractant 3 (combination of a sex pheromone and a synthetic kairomone) according to the present invention was obtained by mixing the mixture with a mixture mixed at a ratio of 19:11.

[0049]

Embodiment 4

(R, Z) -5-(-)-(Oct-1-
(Enyl) oxacyclopentan-2-one, (R, Z)
By mixing -5-(-)-(dec-1-enyl) oxacyclopentan-2-one in a ratio of 8: 2,
A sex-inducing component of Hirata-mog-kogane was synthesized.

By mixing the sex-inducing component of Hirata-mog-mogane with a mixture of anethole, geraniol, and phenethylpropionate in a ratio of 9.0: 0.5: 0.5, An attractant 4 (combination of a sex pheromone and a food type lure) according to the invention was obtained.

[0052]

[Test Example 1]

As the present invention section 1, Japan Tobacco Inc. was prepared by impregnating the attractant 1 obtained in Example 1 into a pellet comprising ethylene-vinyl acetate copolymer or a device comprising ES fiber and polyethylene film. It was set on a funnel-type insect insect made by the company.

Then, 10 funnel-type insect traps were actually stored at Edozaki Country Club in Ibaraki Prefecture on May 3rd.
The insect trap test was conducted for 7 days until 1993 (1993), and the average number of insect traps per day (male and female adults) per day based on the total number of Pleurotus arborea trapped during this period
I asked.

The average number of insect traps was determined in the same manner as in the inventive section 1 except that the attractant 2 obtained in Example 2 was used as the inventive section 2.

As a control 1, holotrichia pa (Holotrichia pa) which is said to attract various chafers
rallera), the main component of the sex pheromone LIME (Li
The average number of insect traps was determined in the same manner as in the section 1 of the present invention except that soleucine methyl ester) (Leal et al., 1993) was used.

The results of the test are shown below.

The number of the present invention group 1 was 100.

The number of the present invention group 2 was 130.

The number of the control group 1 was 16 animals.

From the above results, it can be seen that the attractant 1 (based on synthetic kairomone) and the attractant 2 (based on food type lure) have a better attraction effect than LIME which attracts various scarabs.

[0062]

[Test Example 2]

As the present invention section 3, Japan Tobacco Inc. was prepared by impregnating the attractant 3 obtained in Example 3 into a pellet made of ethylene-vinyl acetate copolymer or a device made of ES fiber and polyethylene film. It was set in a funnel-type insect insect.

Then, 10 funnel-type insect traps were actually stored at Edozaki Country Club in Ibaraki Prefecture from May 3 to 9
The insect trap test was conducted for 7 days until 1993 (1993), and the average number of insect traps per day (male and female adults) per day based on the total number of Pleurotus arborea trapped during this period
I asked.

As control group 2, (R, Z) -5-(-)
-(Oct-1-enyl) oxacyclopentane-2-
Except for using a sex-inducing component contained in Hirata-kogane, which is a mixture of on, (R, Z) -5-(-)-(dec-1-enyl) oxacyclopentan-2-one in a ratio of 8: 2. In the same manner as in the section 3 of the present invention, the average number of insects was determined.

The results of the test are shown below.

The number of the present invention group 3 was 277.

The number of the control group 2 was 107.

From the above results, the attractant 3 (combination of the sex pheromone and the synthetic kairomone) has an excellent attraction effect as compared with the case where the sex pheromone of Hirata moss is used alone. A synergistic effect was confirmed on the pheromone.

[0070]

[Test Example 3]

In the present invention section 4, Japan Tobacco Inc. was prepared by impregnating the attractant 3 obtained in Example 3 into a pellet consisting of a copolymer of ethylene-vinyl acetate or a device consisting of an ES fiber and a polyethylene film. It was set on a funnel-type insect insect made by the company.

Then, ten such funnel-type insect traps were actually installed at a golf course in Chiba Prefecture for seven days from May 3 to 9 (1993), and a trapping test was conducted. The average number of adult insects per day for each insect trap (male and female)
I asked.

The average number of insect traps was determined in the same manner as in the inventive section 4 except that the attractant 1 obtained in Example 1 was used as the inventive section 5.

The results of the test are shown below.

In the plot 4 of the present invention, there were 72 males and 6 females.

In the present invention section 5, there were two males and four females.

From the above results, it can be confirmed that the attractant 3 (combination of the sex pheromone and the synthetic kairomone) has an attracting effect, especially for males.

[0078]

[Test Example 4]

As the present invention section 6, Japan Tobacco Inc. was prepared by impregnating the attractant 4 obtained in Example 4 into a pellet comprising ethylene-vinyl acetate copolymer or a device comprising ES fiber and polyethylene film. It was set on a funnel-type insect insect made by the company.

Then, ten such funnel-type insect traps were actually installed at a golf course in Chiba Prefecture for seven days from May 3 to 9 (1993), and a trapping test was conducted. The average number of adult insects per day for each insect trap (male and female)
I asked.

As a control group 3, (R, Z) -5-(-)
-(Oct-1-enyl) oxacyclopentane-2-
Except for using a sex-inducing component contained in Hirata-kogane, which is a mixture of on, (R, Z) -5-(-)-(dec-1-enyl) oxacyclopentan-2-one in a ratio of 8: 2. In the same manner as in the section of the present invention 6, the average number of insect traps was determined.

The results of the test are shown below.

In the plot 6 of the present invention, there were 97 males and 8 females.

In control group 3, 95 males and 2 females were used.

From the above results, the attractant 4 (combination of sex pheromone and food type lure) was particularly
It can be confirmed that it has a strong attraction effect for females.

[0086]

【The invention's effect】

The attractant of the present invention has a sufficient attracting effect on Hirata moth.

Further, the attractant containing a sex-attracting component of Hirata moth, according to the present invention, has a stronger attraction effect than when the sex pheromone is used alone.

In particular, an attractant in which a sex pheromone and a food-type lure are combined has a strong attracting effect, especially for females of Hirata moss.

The attractant obtained by combining the sex pheromone and the synthetic kairomone has a strong attracting effect, especially for males of Hirata aeruginosa.

Therefore, by using the attractant of the present invention,
It will be possible to develop more effective methods for monitoring the emergence and fate of large numbers of adults than using the sex pheromone of Hirata moss alone.

──────────────────────────────────────────────────続 き Continuation of the front page (51) Int.Cl. ⁶ Identification number Agency reference number FI Technical display location A01N 37/10 A01N 37/10 37/34 107 9450-4H 37/34 107 43/08 43/08 H 65/00 65/00 A

Claims (7)

(57) [Claims] [Claim 1] An attractant for Hirata moth, comprising a mixture of anethole, geraniol, and phenethyl propionate. 2. An attractant for Hirata moth, comprising an extract of dandelion flowers. 3. A mixture of cis-3-hexenyl acetate, benzaldehyde, phenylacetaldehyde, benzyl alcohol, phenethyl alcohol, phenylacetonitrile, benzylbenzoate,
Attractant for Hirata-Akogane. 4. An attractant for Hirata moth, comprising a mixture of anethole, geraniol, and phenethyl propionate, and a sex attraction component of Hirata moth. 5. An attractive agent of Hirata moth, comprising a flower extract of Dandelion dandelion and a sex attraction component of Hirata moth. 6. Anethole, geraniol, phenethyl propionate, (R, Z) -5-(−)-(oct-
1-enyl) oxacyclopentan-2-one, (R,
Z) -5-(-)-(Dec-1-enyl) oxacyclopentan-2-one, an attractant for Pleurotus terrestris. 7. cis-3-hexenyl acetate, benzaldehyde, phenylacetaldehyde, benzyl alcohol, phenethyl alcohol, phenylacetonitrile, benzylbenzoate, (R, Z) -5
(-)-(Oct-1-enyl) oxacyclopentan-2-one, (R, Z) -5-(-)-(dec-1-
Enyl) oxacyclopentan-2-one,
Attractant for Hirata-Akogane.