JP2025510151A - アダプターダイマーの形成を阻害するための方法、組成物、及びキット - Google Patents
アダプターダイマーの形成を阻害するための方法、組成物、及びキット Download PDFInfo
- Publication number
- JP2025510151A JP2025510151A JP2024556472A JP2024556472A JP2025510151A JP 2025510151 A JP2025510151 A JP 2025510151A JP 2024556472 A JP2024556472 A JP 2024556472A JP 2024556472 A JP2024556472 A JP 2024556472A JP 2025510151 A JP2025510151 A JP 2025510151A
- Authority
- JP
- Japan
- Prior art keywords
- blocker oligonucleotide
- oligonucleotide
- adaptor
- nucleotides
- blocker
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263323778P | 2022-03-25 | 2022-03-25 | |
| US63/323,778 | 2022-03-25 | ||
| PCT/US2023/016295 WO2023183611A1 (en) | 2022-03-25 | 2023-03-24 | Methods, compositions and kits for inhibiting formation of adapter dimers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2025510151A true JP2025510151A (ja) | 2025-04-14 |
| JP2025510151A5 JP2025510151A5 (https=) | 2026-02-25 |
Family
ID=86271326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024556472A Pending JP2025510151A (ja) | 2022-03-25 | 2023-03-24 | アダプターダイマーの形成を阻害するための方法、組成物、及びキット |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20230323341A1 (https=) |
| EP (1) | EP4499860A1 (https=) |
| JP (1) | JP2025510151A (https=) |
| CN (1) | CN119325511A (https=) |
| AU (1) | AU2023239149A1 (https=) |
| WO (1) | WO2023183611A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025079565A1 (ja) * | 2023-10-13 | 2025-04-17 | アークレイ株式会社 | ライゲーション方法、アダプターダイマー形成の抑制方法、ｎｇｓライブラリの調製方法、ｎｇｓライブラリ調製キット、及び３'アダプター |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8883421B2 (en) * | 2010-09-10 | 2014-11-11 | New England Biolabs, Inc. | Method for reducing adapter-dimer formation |
| US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
| WO2016164866A1 (en) * | 2015-04-10 | 2016-10-13 | Hudsonalpha Institute For Biotechnology | METHOD FOR BLOCKING miRNA |
| CN114040975A (zh) * | 2019-06-07 | 2022-02-11 | 生物辐射实验室股份有限公司 | 每液滴多个珠的解决方案 |
| EP4150106A4 (en) * | 2020-05-15 | 2024-07-10 | Integrated Dna Technologies, Inc. | LIGATURE-COUPLED PCR METHODS |
| WO2022046797A1 (en) * | 2020-08-25 | 2022-03-03 | Twist Bioscience Corporation | Compositions and methods for library sequencing |
-
2023
- 2023-03-24 US US18/126,074 patent/US20230323341A1/en active Pending
- 2023-03-24 AU AU2023239149A patent/AU2023239149A1/en active Pending
- 2023-03-24 WO PCT/US2023/016295 patent/WO2023183611A1/en not_active Ceased
- 2023-03-24 CN CN202380030039.5A patent/CN119325511A/zh active Pending
- 2023-03-24 EP EP23720194.2A patent/EP4499860A1/en active Pending
- 2023-03-24 JP JP2024556472A patent/JP2025510151A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023183611A1 (en) | 2023-09-28 |
| CN119325511A (zh) | 2025-01-17 |
| EP4499860A1 (en) | 2025-02-05 |
| AU2023239149A1 (en) | 2024-09-19 |
| US20230323341A1 (en) | 2023-10-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20260216 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20260216 |