AU2023239149A1 - Methods, compositions and kits for inhibiting formation of adapter dimers - Google Patents

Methods, compositions and kits for inhibiting formation of adapter dimers Download PDF

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Publication number
AU2023239149A1
AU2023239149A1 AU2023239149A AU2023239149A AU2023239149A1 AU 2023239149 A1 AU2023239149 A1 AU 2023239149A1 AU 2023239149 A AU2023239149 A AU 2023239149A AU 2023239149 A AU2023239149 A AU 2023239149A AU 2023239149 A1 AU2023239149 A1 AU 2023239149A1
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AU
Australia
Prior art keywords
adapter
blocker oligonucleotide
blocker
nucleotides
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2023239149A
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English (en)
Inventor
Kevin D. Allen
Eric Castro
Kerry Gunning
Allyson LABAS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bioo Scientific Corp
Original Assignee
Bioo Scientific Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioo Scientific Corp filed Critical Bioo Scientific Corp
Publication of AU2023239149A1 publication Critical patent/AU2023239149A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2023239149A 2022-03-25 2023-03-24 Methods, compositions and kits for inhibiting formation of adapter dimers Pending AU2023239149A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202263323778P 2022-03-25 2022-03-25
US63/323,778 2022-03-25
PCT/US2023/016295 WO2023183611A1 (en) 2022-03-25 2023-03-24 Methods, compositions and kits for inhibiting formation of adapter dimers

Publications (1)

Publication Number Publication Date
AU2023239149A1 true AU2023239149A1 (en) 2024-09-19

Family

ID=86271326

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2023239149A Pending AU2023239149A1 (en) 2022-03-25 2023-03-24 Methods, compositions and kits for inhibiting formation of adapter dimers

Country Status (6)

Country Link
US (1) US20230323341A1 (https=)
EP (1) EP4499860A1 (https=)
JP (1) JP2025510151A (https=)
CN (1) CN119325511A (https=)
AU (1) AU2023239149A1 (https=)
WO (1) WO2023183611A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025079565A1 (ja) * 2023-10-13 2025-04-17 アークレイ株式会社 ライゲーション方法、アダプターダイマー形成の抑制方法、ｎｇｓライブラリの調製方法、ｎｇｓライブラリ調製キット、及び３'アダプター

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883421B2 (en) * 2010-09-10 2014-11-11 New England Biolabs, Inc. Method for reducing adapter-dimer formation
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2016164866A1 (en) * 2015-04-10 2016-10-13 Hudsonalpha Institute For Biotechnology METHOD FOR BLOCKING miRNA
CN114040975A (zh) * 2019-06-07 2022-02-11 生物辐射实验室股份有限公司 每液滴多个珠的解决方案
EP4150106A4 (en) * 2020-05-15 2024-07-10 Integrated Dna Technologies, Inc. LIGATURE-COUPLED PCR METHODS
WO2022046797A1 (en) * 2020-08-25 2022-03-03 Twist Bioscience Corporation Compositions and methods for library sequencing

Also Published As

Publication number Publication date
WO2023183611A1 (en) 2023-09-28
CN119325511A (zh) 2025-01-17
EP4499860A1 (en) 2025-02-05
US20230323341A1 (en) 2023-10-12
JP2025510151A (ja) 2025-04-14

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