CN119325511A - 用于抑制接头二聚体形成的方法、组合物和试剂盒 - Google Patents

用于抑制接头二聚体形成的方法、组合物和试剂盒 Download PDF

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Publication number
CN119325511A
CN119325511A CN202380030039.5A CN202380030039A CN119325511A CN 119325511 A CN119325511 A CN 119325511A CN 202380030039 A CN202380030039 A CN 202380030039A CN 119325511 A CN119325511 A CN 119325511A
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CN
China
Prior art keywords
linker
blocker oligonucleotide
oligonucleotide
blocker
nucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380030039.5A
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English (en)
Chinese (zh)
Inventor
K·D·艾伦
K·冈宁
A·拉巴斯
E·卡斯特罗
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Bioo Scientific Corp
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Bioo Scientific Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Bioo Scientific Corp filed Critical Bioo Scientific Corp
Publication of CN119325511A publication Critical patent/CN119325511A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202380030039.5A 2022-03-25 2023-03-24 用于抑制接头二聚体形成的方法、组合物和试剂盒 Pending CN119325511A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202263323778P 2022-03-25 2022-03-25
US63/323,778 2022-03-25
PCT/US2023/016295 WO2023183611A1 (en) 2022-03-25 2023-03-24 Methods, compositions and kits for inhibiting formation of adapter dimers

Publications (1)

Publication Number Publication Date
CN119325511A true CN119325511A (zh) 2025-01-17

Family

ID=86271326

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380030039.5A Pending CN119325511A (zh) 2022-03-25 2023-03-24 用于抑制接头二聚体形成的方法、组合物和试剂盒

Country Status (6)

Country Link
US (1) US20230323341A1 (https=)
EP (1) EP4499860A1 (https=)
JP (1) JP2025510151A (https=)
CN (1) CN119325511A (https=)
AU (1) AU2023239149A1 (https=)
WO (1) WO2023183611A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025079565A1 (ja) * 2023-10-13 2025-04-17 アークレイ株式会社 ライゲーション方法、アダプターダイマー形成の抑制方法、ｎｇｓライブラリの調製方法、ｎｇｓライブラリ調製キット、及び３'アダプター

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883421B2 (en) * 2010-09-10 2014-11-11 New England Biolabs, Inc. Method for reducing adapter-dimer formation
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2016164866A1 (en) * 2015-04-10 2016-10-13 Hudsonalpha Institute For Biotechnology METHOD FOR BLOCKING miRNA
CN114040975A (zh) * 2019-06-07 2022-02-11 生物辐射实验室股份有限公司 每液滴多个珠的解决方案
EP4150106A4 (en) * 2020-05-15 2024-07-10 Integrated Dna Technologies, Inc. LIGATURE-COUPLED PCR METHODS
WO2022046797A1 (en) * 2020-08-25 2022-03-03 Twist Bioscience Corporation Compositions and methods for library sequencing

Also Published As

Publication number Publication date
WO2023183611A1 (en) 2023-09-28
EP4499860A1 (en) 2025-02-05
AU2023239149A1 (en) 2024-09-19
US20230323341A1 (en) 2023-10-12
JP2025510151A (ja) 2025-04-14

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