JP2024519113A - 生物学的試料中の単一細胞を位置特定及びプロファイリングするための方法及び構築体 - Google Patents
生物学的試料中の単一細胞を位置特定及びプロファイリングするための方法及び構築体 Download PDFInfo
- Publication number
- JP2024519113A JP2024519113A JP2023572114A JP2023572114A JP2024519113A JP 2024519113 A JP2024519113 A JP 2024519113A JP 2023572114 A JP2023572114 A JP 2023572114A JP 2023572114 A JP2023572114 A JP 2023572114A JP 2024519113 A JP2024519113 A JP 2024519113A
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- beads
- bead
- construct
- visually detectable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/102—Multiple non-interacting labels
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP21175329.8 | 2021-05-21 | ||
| EP21175329.8A EP4092133A1 (en) | 2021-05-21 | 2021-05-21 | Methods and constructs for locating and profiling single cells in a biological sample |
| PCT/FI2022/050347 WO2022243609A1 (en) | 2021-05-21 | 2022-05-20 | Methods and constructs for locating and profiling single cells in a biological sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2024519113A true JP2024519113A (ja) | 2024-05-08 |
| JP2024519113A5 JP2024519113A5 (https=) | 2025-05-28 |
Family
ID=76059773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023572114A Pending JP2024519113A (ja) | 2021-05-21 | 2022-05-20 | 生物学的試料中の単一細胞を位置特定及びプロファイリングするための方法及び構築体 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20240229104A1 (https=) |
| EP (3) | EP4092133A1 (https=) |
| JP (1) | JP2024519113A (https=) |
| KR (1) | KR20240012460A (https=) |
| CN (1) | CN117425737A (https=) |
| AU (1) | AU2022278653A1 (https=) |
| BR (1) | BR112023024326A2 (https=) |
| CA (1) | CA3220616A1 (https=) |
| ES (1) | ES3035340T3 (https=) |
| IL (1) | IL308636A (https=) |
| PL (1) | PL4244376T3 (https=) |
| WO (1) | WO2022243609A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4092133A1 (en) * | 2021-05-21 | 2022-11-23 | SCellex Oy | Methods and constructs for locating and profiling single cells in a biological sample |
| WO2024182184A1 (en) * | 2023-02-28 | 2024-09-06 | Becton, Dickinson And Company | Dual indexing particle labels for obtaining linked single cell cytometric and sequence data |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107873054B (zh) | 2014-09-09 | 2022-07-12 | 博德研究所 | 用于复合单细胞核酸分析的基于微滴的方法和设备 |
| WO2016138496A1 (en) * | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| US20220403374A1 (en) * | 2019-09-03 | 2022-12-22 | Flexomics, Llc | Optically readable barcodes and systems and methods for characterizing molecular interactions |
| EP4092133A1 (en) * | 2021-05-21 | 2022-11-23 | SCellex Oy | Methods and constructs for locating and profiling single cells in a biological sample |
-
2021
- 2021-05-21 EP EP21175329.8A patent/EP4092133A1/en not_active Withdrawn
-
2022
- 2022-05-20 JP JP2023572114A patent/JP2024519113A/ja active Pending
- 2022-05-20 EP EP22726267.2A patent/EP4244376B1/en active Active
- 2022-05-20 CA CA3220616A patent/CA3220616A1/en active Pending
- 2022-05-20 KR KR1020237043705A patent/KR20240012460A/ko active Pending
- 2022-05-20 BR BR112023024326A patent/BR112023024326A2/pt unknown
- 2022-05-20 IL IL308636A patent/IL308636A/en unknown
- 2022-05-20 US US18/562,871 patent/US20240229104A1/en active Pending
- 2022-05-20 AU AU2022278653A patent/AU2022278653A1/en active Pending
- 2022-05-20 PL PL22726267.2T patent/PL4244376T3/pl unknown
- 2022-05-20 CN CN202280036500.3A patent/CN117425737A/zh active Pending
- 2022-05-20 ES ES22726267T patent/ES3035340T3/es active Active
- 2022-05-20 EP EP25183432.1A patent/EP4596710A3/en active Pending
- 2022-05-20 WO PCT/FI2022/050347 patent/WO2022243609A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| IL308636A (en) | 2024-01-01 |
| CN117425737A (zh) | 2024-01-19 |
| BR112023024326A2 (pt) | 2024-02-06 |
| AU2022278653A9 (en) | 2024-01-04 |
| EP4596710A2 (en) | 2025-08-06 |
| EP4092133A1 (en) | 2022-11-23 |
| WO2022243609A1 (en) | 2022-11-24 |
| EP4244376C0 (en) | 2025-06-18 |
| CA3220616A1 (en) | 2022-11-24 |
| ES3035340T3 (en) | 2025-09-01 |
| EP4596710A3 (en) | 2025-11-12 |
| US20240229104A1 (en) | 2024-07-11 |
| KR20240012460A (ko) | 2024-01-29 |
| PL4244376T3 (pl) | 2025-08-18 |
| EP4244376A1 (en) | 2023-09-20 |
| AU2022278653A1 (en) | 2023-12-14 |
| EP4244376B1 (en) | 2025-06-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Asp et al. | Spatially resolved transcriptomes—next generation tools for tissue exploration | |
| US20240093291A1 (en) | Spatial genomics with single cell resolution | |
| US20230032082A1 (en) | Spatial barcoding | |
| US9709479B2 (en) | Method and apparatus for tracking cell identity | |
| US9850482B2 (en) | Heterologous DNA barcoding method | |
| WO2019104337A1 (en) | Rna printing and sequencing devices, methods, and systems | |
| CN105264088A (zh) | 提高鉴定细胞中的多个表位的动态范围 | |
| Battersby et al. | Optical encoding of microbeads for gene screening: alternatives to microarrays | |
| JP6846832B2 (ja) | 一細胞の非破壊的計測情報とゲノム関連情報とを一体的に検出する方法 | |
| EP4244376B1 (en) | Methods and constructs for locating and profiling single cells in a biological sample | |
| Li et al. | Spatial transcriptomics: new dimension of understanding biological complexity | |
| US12577556B2 (en) | Perturbation beads for use in assays | |
| WO2000031536A2 (en) | Detecting structural or synthetic information about chemical compounds | |
| JP2022538835A (ja) | 高容量分子検出 | |
| WO2023059935A1 (en) | Fluorescent barcoding of microparticles | |
| JP2024519113A5 (https=) | ||
| FI115139B (fi) | Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen | |
| HK40104853A (zh) | 用於定位和分析生物样品中的单细胞的方法和构建体 | |
| US20220025430A1 (en) | Sequence based imaging | |
| US20240361310A1 (en) | Capture Beads for Use in Assays | |
| US20250011847A1 (en) | Methods for indexing single cells and nuclei using nanoball combinatorial identifiers | |
| US20240301490A1 (en) | High throughput single cell based assay for capturing genomic information for functional and imaging analysis and methods of use | |
| JP3944576B2 (ja) | マイクロアレイを用いたアプタマーの取得方法 | |
| WO2025160235A1 (en) | Capture beads for use in assays | |
| WO2025159784A1 (en) | Capture beads for use in assays |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20250520 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20250520 |