CN117425737A - 用于定位和分析生物样品中的单细胞的方法和构建体 - Google Patents
用于定位和分析生物样品中的单细胞的方法和构建体 Download PDFInfo
- Publication number
- CN117425737A CN117425737A CN202280036500.3A CN202280036500A CN117425737A CN 117425737 A CN117425737 A CN 117425737A CN 202280036500 A CN202280036500 A CN 202280036500A CN 117425737 A CN117425737 A CN 117425737A
- Authority
- CN
- China
- Prior art keywords
- sequence
- oligonucleotide
- beads
- bead
- construct
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/102—Multiple non-interacting labels
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP21175329.8 | 2021-05-21 | ||
| EP21175329.8A EP4092133A1 (en) | 2021-05-21 | 2021-05-21 | Methods and constructs for locating and profiling single cells in a biological sample |
| PCT/FI2022/050347 WO2022243609A1 (en) | 2021-05-21 | 2022-05-20 | Methods and constructs for locating and profiling single cells in a biological sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117425737A true CN117425737A (zh) | 2024-01-19 |
Family
ID=76059773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202280036500.3A Pending CN117425737A (zh) | 2021-05-21 | 2022-05-20 | 用于定位和分析生物样品中的单细胞的方法和构建体 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20240229104A1 (https=) |
| EP (3) | EP4092133A1 (https=) |
| JP (1) | JP2024519113A (https=) |
| KR (1) | KR20240012460A (https=) |
| CN (1) | CN117425737A (https=) |
| AU (1) | AU2022278653A1 (https=) |
| BR (1) | BR112023024326A2 (https=) |
| CA (1) | CA3220616A1 (https=) |
| ES (1) | ES3035340T3 (https=) |
| IL (1) | IL308636A (https=) |
| PL (1) | PL4244376T3 (https=) |
| WO (1) | WO2022243609A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4092133A1 (en) * | 2021-05-21 | 2022-11-23 | SCellex Oy | Methods and constructs for locating and profiling single cells in a biological sample |
| WO2024182184A1 (en) * | 2023-02-28 | 2024-09-06 | Becton, Dickinson And Company | Dual indexing particle labels for obtaining linked single cell cytometric and sequence data |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107873054B (zh) | 2014-09-09 | 2022-07-12 | 博德研究所 | 用于复合单细胞核酸分析的基于微滴的方法和设备 |
| WO2016138496A1 (en) * | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| US20220403374A1 (en) * | 2019-09-03 | 2022-12-22 | Flexomics, Llc | Optically readable barcodes and systems and methods for characterizing molecular interactions |
| EP4092133A1 (en) * | 2021-05-21 | 2022-11-23 | SCellex Oy | Methods and constructs for locating and profiling single cells in a biological sample |
-
2021
- 2021-05-21 EP EP21175329.8A patent/EP4092133A1/en not_active Withdrawn
-
2022
- 2022-05-20 JP JP2023572114A patent/JP2024519113A/ja active Pending
- 2022-05-20 EP EP22726267.2A patent/EP4244376B1/en active Active
- 2022-05-20 CA CA3220616A patent/CA3220616A1/en active Pending
- 2022-05-20 KR KR1020237043705A patent/KR20240012460A/ko active Pending
- 2022-05-20 BR BR112023024326A patent/BR112023024326A2/pt unknown
- 2022-05-20 IL IL308636A patent/IL308636A/en unknown
- 2022-05-20 US US18/562,871 patent/US20240229104A1/en active Pending
- 2022-05-20 AU AU2022278653A patent/AU2022278653A1/en active Pending
- 2022-05-20 PL PL22726267.2T patent/PL4244376T3/pl unknown
- 2022-05-20 CN CN202280036500.3A patent/CN117425737A/zh active Pending
- 2022-05-20 ES ES22726267T patent/ES3035340T3/es active Active
- 2022-05-20 EP EP25183432.1A patent/EP4596710A3/en active Pending
- 2022-05-20 WO PCT/FI2022/050347 patent/WO2022243609A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| IL308636A (en) | 2024-01-01 |
| BR112023024326A2 (pt) | 2024-02-06 |
| AU2022278653A9 (en) | 2024-01-04 |
| EP4596710A2 (en) | 2025-08-06 |
| EP4092133A1 (en) | 2022-11-23 |
| WO2022243609A1 (en) | 2022-11-24 |
| EP4244376C0 (en) | 2025-06-18 |
| CA3220616A1 (en) | 2022-11-24 |
| JP2024519113A (ja) | 2024-05-08 |
| ES3035340T3 (en) | 2025-09-01 |
| EP4596710A3 (en) | 2025-11-12 |
| US20240229104A1 (en) | 2024-07-11 |
| KR20240012460A (ko) | 2024-01-29 |
| PL4244376T3 (pl) | 2025-08-18 |
| EP4244376A1 (en) | 2023-09-20 |
| AU2022278653A1 (en) | 2023-12-14 |
| EP4244376B1 (en) | 2025-06-18 |
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