JP2024500105A5 - - Google Patents

Info

Publication number
JP2024500105A5
JP2024500105A5 JP2023536435A JP2023536435A JP2024500105A5 JP 2024500105 A5 JP2024500105 A5 JP 2024500105A5 JP 2023536435 A JP2023536435 A JP 2023536435A JP 2023536435 A JP2023536435 A JP 2023536435A JP 2024500105 A5 JP2024500105 A5 JP 2024500105A5
Authority
JP
Japan
Prior art keywords
paqci
dna
ligase
oligonucleotide
reaction mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023536435A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024500105A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2021/010063 external-priority patent/WO2022132198A2/en
Publication of JP2024500105A publication Critical patent/JP2024500105A/ja
Publication of JP2024500105A5 publication Critical patent/JP2024500105A5/ja
Pending legal-status Critical Current

Links

JP2023536435A 2020-12-15 2021-12-15 ポリヌクレオチドの改善されたインビトロアセンブリーのための組成物及び方法 Pending JP2024500105A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063125530P 2020-12-15 2020-12-15
US63/125,530 2020-12-15
US202163213807P 2021-06-23 2021-06-23
US202163213859P 2021-06-23 2021-06-23
US63/213,859 2021-06-23
US63/213,807 2021-06-23
PCT/US2021/010063 WO2022132198A2 (en) 2020-12-15 2021-12-15 Compositions and methods for improved in vitro assembly of polynucleotides

Publications (2)

Publication Number Publication Date
JP2024500105A JP2024500105A (ja) 2024-01-04
JP2024500105A5 true JP2024500105A5 (https=) 2024-12-04

Family

ID=80682842

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023536435A Pending JP2024500105A (ja) 2020-12-15 2021-12-15 ポリヌクレオチドの改善されたインビトロアセンブリーのための組成物及び方法

Country Status (4)

Country Link
EP (1) EP4263827A2 (https=)
JP (1) JP2024500105A (https=)
KR (1) KR20230121625A (https=)
WO (1) WO2022132198A2 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12435332B2 (en) 2018-10-19 2025-10-07 New England Biolabs, Inc. Ordered assembly of multiple DNA fragments
US12188011B2 (en) 2018-10-19 2025-01-07 New England Biolabs, Inc. Compositions and methods for improved in vitro assembly of polynucleotides
WO2023001141A1 (en) * 2021-07-23 2023-01-26 Shanghai Zion Pharma Co. Limited Kras g12d inhibitors and uses thereof
CN116004682B (zh) * 2022-08-19 2026-01-16 中国人民解放军军事科学院军事医学研究院 一种快速无痕制备含长多聚腺嘌呤的mRNA的方法及应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5248600A (en) * 1990-12-14 1993-09-28 The University Of North Carolina At Chapel Hill Method of cleaving DNA
JP4663988B2 (ja) 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Dna操作のための方法および組成物
JP2013514758A (ja) * 2008-12-23 2013-05-02 ニユー・イングランド・バイオレイブス・インコーポレイテツド 修飾dnaを切断するための組成物、方法および関連する使用
US20210383891A1 (en) * 2018-10-19 2021-12-09 New England Biolabs, Inc. Improved Ordered Assembly of Multiple DNA Fragments
CN209999070U (zh) 2019-03-14 2020-01-31 南京德朔实业有限公司 电动螺丝批

Similar Documents

Publication Publication Date Title
JP2024500105A5 (https=)
US9255291B2 (en) Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
CN105705515B (zh) 多种用于dna操作的转座酶适体
EP2880182B1 (en) Recombinase mediated targeted dna enrichment for next generation sequencing
US20140045728A1 (en) Orthogonal Amplification and Assembly of Nucleic Acid Sequences
US20180057876A1 (en) Preparation of adapter-ligated amplicons
US12065684B2 (en) Demand synthesis of polynucleotide sequences
JP2023507529A (ja) ペアド-エンドシークエンシングのための制御された鎖置換
JP2023519782A (ja) 標的化された配列決定の方法
US20130045894A1 (en) Method for Amplification of Target Nucleic Acids Using a Multi-Primer Approach
US10655170B2 (en) Coupling adaptors to a target nucleic acid
JP2021048883A (ja) Pegを介した核酸分子のアセンブリ
US10557135B2 (en) Sequence tags
US9695417B2 (en) Method for large-scale synthesis of long-chain nucleic acid molecule
US20240271328A1 (en) Rapid library construction for high throughput sequencing
EP3865584A1 (en) Method for constructing sequencing library
EP4516903A1 (en) Sgrna sequencing linker and use thereof
RU2809771C2 (ru) Композиции и способы для улучшения обогащения библиотек
US20220403553A1 (en) Method for screening libraries
TW202540416A (zh) 建構標的核酸之多核苷酸庫的方法
CN118355129A (zh) 捕获crispr核酸内切酶切割产物的方法
WO2024120262A1 (zh) 一种可剪切环状引物及其试剂盒与扩增方法
WO2023085232A1 (ja) 改良されたライブラリー調製方法
WO2024073034A1 (en) Simplified sequencing library preparation for dna
CN121592760A (zh) 一种dI修饰引物促进核酸扩增反应的方法及其应用