KR20230121625A - 폴리뉴클레오타이드의 개선된 시험관내 조립을 위한조성물 및 방법 - Google Patents

폴리뉴클레오타이드의 개선된 시험관내 조립을 위한조성물 및 방법 Download PDF

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Publication number
KR20230121625A
KR20230121625A KR1020237024116A KR20237024116A KR20230121625A KR 20230121625 A KR20230121625 A KR 20230121625A KR 1020237024116 A KR1020237024116 A KR 1020237024116A KR 20237024116 A KR20237024116 A KR 20237024116A KR 20230121625 A KR20230121625 A KR 20230121625A
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dna
overhangs
ligase
fragments
overhang
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Korean (ko)
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그레고리 로만
블라디미르 포타포브
존 엠. 프라이어
레베카 쿠체라
카타리나 빌로티
리챠드 디. 모건
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뉴 잉글랜드 바이오랩스, 인크
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Publication of KR20230121625A publication Critical patent/KR20230121625A/ko
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1031Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/20Sequence assembly

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
KR1020237024116A 2020-12-15 2021-12-15 폴리뉴클레오타이드의 개선된 시험관내 조립을 위한조성물 및 방법 Pending KR20230121625A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063125530P 2020-12-15 2020-12-15
US63/125,530 2020-12-15
US202163213807P 2021-06-23 2021-06-23
US202163213859P 2021-06-23 2021-06-23
US63/213,859 2021-06-23
US63/213,807 2021-06-23
PCT/US2021/010063 WO2022132198A2 (en) 2020-12-15 2021-12-15 Compositions and methods for improved in vitro assembly of polynucleotides

Publications (1)

Publication Number Publication Date
KR20230121625A true KR20230121625A (ko) 2023-08-18

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KR1020237024116A Pending KR20230121625A (ko) 2020-12-15 2021-12-15 폴리뉴클레오타이드의 개선된 시험관내 조립을 위한조성물 및 방법

Country Status (4)

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EP (1) EP4263827A2 (https=)
JP (1) JP2024500105A (https=)
KR (1) KR20230121625A (https=)
WO (1) WO2022132198A2 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12435332B2 (en) 2018-10-19 2025-10-07 New England Biolabs, Inc. Ordered assembly of multiple DNA fragments
US12188011B2 (en) 2018-10-19 2025-01-07 New England Biolabs, Inc. Compositions and methods for improved in vitro assembly of polynucleotides
WO2023001141A1 (en) * 2021-07-23 2023-01-26 Shanghai Zion Pharma Co. Limited Kras g12d inhibitors and uses thereof
CN116004682B (zh) * 2022-08-19 2026-01-16 中国人民解放军军事科学院军事医学研究院 一种快速无痕制备含长多聚腺嘌呤的mRNA的方法及应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5248600A (en) * 1990-12-14 1993-09-28 The University Of North Carolina At Chapel Hill Method of cleaving DNA
JP4663988B2 (ja) 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Dna操作のための方法および組成物
JP2013514758A (ja) * 2008-12-23 2013-05-02 ニユー・イングランド・バイオレイブス・インコーポレイテツド 修飾dnaを切断するための組成物、方法および関連する使用
US20210383891A1 (en) * 2018-10-19 2021-12-09 New England Biolabs, Inc. Improved Ordered Assembly of Multiple DNA Fragments
CN209999070U (zh) 2019-03-14 2020-01-31 南京德朔实业有限公司 电动螺丝批

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WO2022132198A3 (en) 2022-08-18
JP2024500105A (ja) 2024-01-04
EP4263827A2 (en) 2023-10-25
WO2022132198A2 (en) 2022-06-23

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Patent event date: 20230714

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Patent event code: PA02012R01D

Patent event date: 20241212

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