JP2024500105A - ポリヌクレオチドの改善されたインビトロアセンブリーのための組成物及び方法 - Google Patents

ポリヌクレオチドの改善されたインビトロアセンブリーのための組成物及び方法 Download PDF

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JP2024500105A
JP2024500105A JP2023536435A JP2023536435A JP2024500105A JP 2024500105 A JP2024500105 A JP 2024500105A JP 2023536435 A JP2023536435 A JP 2023536435A JP 2023536435 A JP2023536435 A JP 2023536435A JP 2024500105 A JP2024500105 A JP 2024500105A
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ligation
dna
ligase
overhangs
fragments
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ローマン，グレゴリー
ポタポフ，ブラジーミル
プライアー，ジョン・エム
クセラ，レベッカ
ビロッティ，カタリーナ
モーガン，リチャード・ディー
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ニユー・イングランド・バイオレイブス・インコーポレイテツド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/20—Sequence assembly

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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JP2023536435A 2020-12-15 2021-12-15 ポリヌクレオチドの改善されたインビトロアセンブリーのための組成物及び方法 Pending JP2024500105A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063125530P 2020-12-15 2020-12-15
US63/125,530 2020-12-15
US202163213807P 2021-06-23 2021-06-23
US202163213859P 2021-06-23 2021-06-23
US63/213,859 2021-06-23
US63/213,807 2021-06-23
PCT/US2021/010063 WO2022132198A2 (en) 2020-12-15 2021-12-15 Compositions and methods for improved in vitro assembly of polynucleotides

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JP2024500105A true JP2024500105A (ja) 2024-01-04
JP2024500105A5 JP2024500105A5 (https=) 2024-12-04

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EP (1) EP4263827A2 (https=)
JP (1) JP2024500105A (https=)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024525993A (ja) * 2021-07-23 2024-07-12 スゾウ、ザンロン、ファーマ、リミテッド Ｋｒａｓ ｇ１２ｄ阻害剤およびその使用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12435332B2 (en) 2018-10-19 2025-10-07 New England Biolabs, Inc. Ordered assembly of multiple DNA fragments
US12188011B2 (en) 2018-10-19 2025-01-07 New England Biolabs, Inc. Compositions and methods for improved in vitro assembly of polynucleotides
CN116004682B (zh) * 2022-08-19 2026-01-16 中国人民解放军军事科学院军事医学研究院 一种快速无痕制备含长多聚腺嘌呤的mRNA的方法及应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5418150A (en) * 1990-12-14 1995-05-23 The University Of North Carolina At Chapel Hill Method of cleaving DNA
JP2013514758A (ja) * 2008-12-23 2013-05-02 ニユー・イングランド・バイオレイブス・インコーポレイテツド 修飾ｄｎａを切断するための組成物、方法および関連する使用
WO2020081768A1 (en) * 2018-10-19 2020-04-23 New England Biolabs, Inc. Improved ordered assembly of multiple dna fragments

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4663988B2 (ja) 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Ｄｎａ操作のための方法および組成物
CN209999070U (zh) 2019-03-14 2020-01-31 南京德朔实业有限公司 电动螺丝批

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5418150A (en) * 1990-12-14 1995-05-23 The University Of North Carolina At Chapel Hill Method of cleaving DNA
JP2013514758A (ja) * 2008-12-23 2013-05-02 ニユー・イングランド・バイオレイブス・インコーポレイテツド 修飾ｄｎａを切断するための組成物、方法および関連する使用
WO2020081768A1 (en) * 2018-10-19 2020-04-23 New England Biolabs, Inc. Improved ordered assembly of multiple dna fragments

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANALYTICAL BIOCHEMISTRY, vol. 209, JPN6025044042, 1993, pages 232 - 237, ISSN: 0005717913 *
NUCLEIC ACIDS RESEARCH, vol. 19, no. 19, JPN6025044043, 1991, pages 5139 - 5142, ISSN: 0005717914 *
NUCLEIC ACIDS RESEARCH, vol. 20, no. 19, JPN6025044045, 1992, pages 5127 - 5130, ISSN: 0005717912 *
NUCLEIC ACIDS RESEARCH, vol. Vol.30, No.21, e123, JPN6025044035, 2002, pages 1 - 3, ISSN: 0005717909 *
PNAS, vol. 108, no. 27, JPN6025044044, 2011, pages 11040 - 11045, ISSN: 0005717910 *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 6, JPN6025044041, 2002, pages 4034 - 4041, ISSN: 0005717911 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024525993A (ja) * 2021-07-23 2024-07-12 スゾウ、ザンロン、ファーマ、リミテッド Ｋｒａｓ ｇ１２ｄ阻害剤およびその使用

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KR20230121625A (ko) 2023-08-18
EP4263827A2 (en) 2023-10-25
WO2022132198A2 (en) 2022-06-23

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