JP2024032645A - influenza virus killer - Google Patents

Abstract

An object of the present invention is to provide a new use for honey. SOLUTION: The influenza virus killing agent of the present invention contains honey as an active ingredient. It is preferable that the honey is at least one selected from the group consisting of honeydew honey, ulmo honey, manuka honey, acacia honey, bodaiju honey, and rewarewa honey. [Selection diagram] None

Description

The present invention relates to an influenza virus killer.

Honey is not only edible, but is also known to have various health and beauty effects. For example, honey can be used for various purposes, such as as an antibacterial agent (Patent Document 1), a wound treatment composition, and a cosmetic composition (Patent Documents 2 and 3).

Manuka honey is known to have high antibacterial properties and is used for medical purposes such as a therapeutic agent for periodontitis (Patent Document 4). Furthermore, it is known that manuka honey, astragalus honey, acacia honey, honeydew honey, and buckwheat honey have anti-influenza virus activity that suppresses the proliferation of viruses after infection (Non-Patent Document 1). However, honey is not known to have virucidal activity against influenza viruses.

WO2016/072377 Special Publication No. 2003-516357 JP2003-63984A JP2007-277210A

Ken W., et al., Archives of Medical Research, 2014, 45, 359-365

The present invention aims to provide new uses for honey.

As a result of intensive research to achieve the above object, the present inventors discovered a novel activity in which honey can kill influenza viruses, leading to the completion of the present invention.

That is, the present invention relates to the following inventions, for example.
[1] An influenza-killing virus agent containing honey as an active ingredient.
[2] The influenza virus killing agent according to [1], wherein the honey is at least one selected from the group consisting of honeydew honey, ulmo honey, manuka honey, acacia honey, bodaiju honey, and rewarewa honey.
[3] The influenza virus killing agent according to [1] or [2], which reduces the number of influenza viruses by 50% or more.
[4] A food composition comprising the influenza virus killing agent according to any one of [1] to [3].
[5] A cosmetic product containing the influenza virus killing agent according to any one of [1] to [3].
[6] A disinfectant comprising the influenza virus killing agent according to any one of [1] to [3].
[7] A cleaning agent comprising the influenza virus killing agent according to any one of [1] to [3].

According to the influenza virus killing agent of the present invention, influenza viruses can be killed. Thereby, the risk of influenza virus infection to animals such as humans can be reduced.

The influenza virus killing agent of the present invention contains honey as an active ingredient, and may consist only of honey.

Honey is produced by bees using nectar collected from plant nectar, tree sap, secretions of insects that parasitize plants, etc.

In this embodiment, the type of honey is not particularly limited, and examples include honeydew honey, ulmo honey, manuka honey, acacia honey, bodaiju honey, lewarewa honey, hyakana honey, clover honey, orange honey, astragalus honey, and rosemary honey. Honey, sunflower honey, rape blossom honey, coffee honey, bodaiju honey, cilantro (coriander) honey, Japanese horse chestnut honey, buckwheat honey, hase honey, soft honey, black bean honey, crow honey, etc. can be used. The honey is preferably selected from the group consisting of manuka honey, honeydew honey, ulmo honey, acacia honey, bodaiju honey, and rewarewa honey, and preferably selected from the group consisting of honeydew honey, ulmo honey, and rewarewa honey. More preferred is honeydew honey or ulmo honey. The influenza virus killing agent may contain one type of honey, or may contain two or more types of honey.

Manuka (Leptospermum scoparium) is a woody plant of the Myrtaceae family that grows mainly in New Zealand. Among honeys, manuka-derived honey is known to have particularly high antibacterial properties (Patent Document 4). Manuka honey is graded for its content of methylglyoxal (MG or MGO), a component unique to manuka honey that provides antibacterial effects.

Honeydew honey is called "forest honey" in Europe, and is produced by insects that absorb the sap from various trees (oak Quercus, pinus, fir Abies firma, spruce Picea, etc.) into their bodies. Honey bees collect the sugar that remains in the form of water droplets on the leaves and trunks of trees and make it into honey. Honeydew honey contains more minerals and amino acids than typical flower-derived honey, and like Manuka honey, it is known to have high antibacterial properties.

Ulmo honey is honey that can be collected from the flowers of Ulmo (Eucryphia cordifolia), which belongs to the genus Eucryphia in the family Chunoniaceae. It is called the ``Manuka honey of South America'' because of its strong antibacterial properties.

Acacia honey is honey that can be collected from the flowers of Robinia pseudoacacia, a genus of the Fabaceae family.

Tilia honey is honey that can be collected from the flowers of Tilia miqueliana, a genus of Tilia in the family Malvaceae.

Rewarewa honey is honey that can be collected from the flowers of Knightia excelsa, a member of the Proteaceae family, genus Nightia. Like manuka honey and honeydew honey, it is known to have high antibacterial properties.

The type of bees used to collect honey is not particularly limited. Honey can be obtained, for example, as a beekeeping product according to conventional methods.

While honey is stored in a beehive, it is generally maintained at around 35°C due to the body temperature of the bees. Honey collected from beehives is generally heated, if necessary, to reduce its viscosity to facilitate operations such as transportation, filling, etc., and to melt crystals.

The collected honey may be collected as is, or it may be subjected to purification treatments such as removal of proteins, wax, garbage, bee and hive scum, etc., filtration, extraction with organic solvents, and fractionation before or after heating. , sterilization, concentration, and other treatments may be performed.

Approximately 80% of honey is composed of carbohydrates, and it also contains healthy components such as vitamins, minerals, flavonoids, organic acids, and fatty acids, and the remainder is water. Most of the carbohydrates in honey are glucose and fructose, and it also contains small amounts of oligosaccharides and maltose. The mechanism by which honey kills influenza viruses is not yet known, but the active ingredients may include hydrogen peroxide, methylglyoxal, antibacterial peptides, flavonoids, and organic acids. It is also possible that the effect is due to the synergistic effect of multiple components.

The influenza virus killing effect, that is, the ability to kill influenza viruses, can be evaluated by the rate at which the number of influenza viruses is reduced. Here, the "rate of reducing the number of influenza viruses" refers to the number of influenza viruses before treatment with an influenza virus killer and the number of influenza viruses before treatment with an influenza virus killer, compared to the number of influenza viruses before treatment with an influenza virus killer. This refers to the percentage difference in the number of influenza viruses (reduction in the number of influenza viruses). During evaluation, the contact time with the influenza virus may be sufficient to kill the influenza virus, for example, 5 minutes or more, 10 minutes or more, 30 minutes or more, 1 hour or more, or 2 hours or more. For example, it may be evaluated by the virus reduction rate calculated according to the method shown in the Examples.

The influenza virus killing agent of one embodiment only needs to be able to reduce the number of influenza viruses by 30% or more, such as 40% or more, 50% or more, 55% or more, 60% or more, 70% or more, 80% or more, or 90% or more. % or more is preferable.

The type of influenza virus is not particularly limited, and may be type A, type B, type C, or any of these subtypes. Examples include Hong Kong type (H3N2) and Soviet type (H1N1), with H1N1 being preferred.

The influenza virus killing agent may be in any form capable of exhibiting the ability to kill influenza viruses, and may be composed only of honey, or may be a diluted solution of honey or a formulation made from honey. . The influenza virucidal agent may contain influenza virucidal ingredients other than honey, or other active ingredients or additives. Examples of influenza-killing virus components other than honey include green tea catechins, caffeine, caffeic acid, chlorogenic acid, chlorogenic acid esters, arginine, tannins, plum vinegar polyphenols, and vitamin C. Examples of additives include catechin, green coffee bean extract, caffeine (extract), L-arginine, tannin (extract), persimmon tannin, and vitamin C.

The honey content in the influenza virus killing agent may be any amount that can exhibit the influenza virus killing effect, for example, 0.1% by mass or more, 1% by mass or more, 3% by mass or more, 5% by mass or more, 7% by mass. 10% by mass or more, 15% by mass or more, 20% by mass or more, 25% by mass or more, 30% by mass or more, 35% by mass or more, 40% by mass or more, 45% by mass or more, 50% by mass or more, 55% by mass 60% by mass or more, 65% by mass or more, 70% by mass or more, 75% by mass or more, 80% by mass or more, 85% by mass or more, 90% by mass or more, 95% by mass or more, 97% by mass or more, 98% by mass The content may be 99% by mass or more, 99.5% by mass or more, or 100% by mass. The content of honey in the influenza virus killer is, for example, 99.5% by mass or less, 99% by mass or less, 98% by mass or less, 97% by mass or less, 95% by mass or less, 90% by mass or less, 85% by mass or less, 80% by mass or less mass% or less, 75 mass% or less, 70 mass% or less, 65 mass% or less, 60 mass% or less, 55 mass% or less, 50 mass% or less, 45 mass% or less, 40 mass% or less, 35 mass% or less, 30 % by mass or less, 25% by mass or less, 20% by mass or less, 15% by mass or less, 10% by mass or less, 7% by mass or less, 5% by mass or less, 3% by mass or less, 2% by mass or less, or 1% by mass or less. It's fine.

One embodiment provides a composition comprising an influenza virucidal agent. The composition may be, for example, a food composition, a pharmaceutical composition, a quasi-drug, a cosmetic, a cosmetic raw material, a disinfectant, or a cleaning agent.

The food composition (food) is preferably one in which the tertiary function (physical condition regulation function) of the food is emphasized. Foods with emphasized tertiary functions include, for example, health foods, functional foods, nutritional compositions, nutritional supplements, supplements, health foods, specified health foods, nutritional functional foods, or Examples include foods with functional claims.

The food composition may include food-acceptable ingredients. Ingredients that are acceptable as foods include, for example, minerals, vitamins, flavonoids, quinones, polyphenols, amino acids, nucleic acids, essential fatty acids, cooling agents, binders, sweeteners, disintegrants, lubricants, and colorants. agents, fragrances, stabilizers, preservatives, sustained release modifiers, surfactants, solubilizers, wetting agents, and the like.

If the food composition is a nutritional supplement, supplement, etc., it may be in any form such as solid, liquid, paste, etc. ), capsules, pills, powders, fine granules, granules, solutions, suspensions, emulsions, syrups, pastes, and the like. These can be prepared, for example, by mixing and formulating honey and, if necessary, other ingredients.

The food composition may be obtained by adding honey (influenza virucidal agent) to food, and examples of the food include the following, intermediate products during the manufacturing process, or final product. Products can be mixed with an effective amount of honey to obtain foods used for the above purposes: soft drinks such as coffee, juices and tea drinks, milk drinks, lactic acid drinks, yogurt drinks, carbonated drinks, and Beverages such as alcoholic beverages such as Japanese sake, Western liquors, fruit liquors, and mead; Spreads such as custard cream; Pastes such as fruit paste; Western sweets such as chocolate, donuts, pies, cream puffs, gum, jelly, candies, cookies, cakes, and puddings. ; Japanese sweets such as daifuku, mochi, manju, castella, anmitsu, and yokan; frozen confections such as ice cream, popsicles, and sorbet; cooked food such as curry, beef bowl, porridge, miso soup, soup, meat sauce, pasta, pickles, and jam; Food: Seasonings such as dressings, furikake, umami seasonings, and soup bases.

The content of honey in the food composition is not particularly limited, and may be the same as the content of honey in the influenza virus killer, for example. The method for producing the food composition is not particularly limited, and any known method can be followed as appropriate. For example, a food composition used for the above purpose can be obtained by mixing the above honey with an intermediate product or a final product in the manufacturing process of the food composition.

In addition to honey, the pharmaceutical composition contains pharmaceutically acceptable ingredients such as excipients, binders, lubricants, disintegrants, emulsifiers, surfactants, bases, solubilizing agents, and suspending agents. It may also contain agents. The pharmaceutical composition may be in any form such as solid, liquid, or paste, and may include tablets (including uncoated tablets, sugar-coated tablets, effervescent tablets, film-coated tablets, chewable tablets, troches, etc.), capsules, and pills. , powders, fine granules, granules, solutions, suspensions, emulsions, syrups, pastes, injections (when used, mixed with distilled water or infusions such as amino acid infusions or electrolyte infusions) (including the case where it is prepared as a topical preparation), a topical dosage form such as a liniment or a patch.

The cosmetic raw material may be in any form such as solid, liquid, or paste. Cosmetics may be medicated cosmetics (ie, quasi-drugs). Cosmetics include all cosmetics that can be applied to the skin, mucous membranes, body hair, hair, scalp, nails, teeth, facial skin, lips, and other areas of animals (particularly humans).

Cosmetics may contain ingredients acceptable as cosmetics in addition to honey (influenza virus killer). Ingredients that are acceptable for cosmetics include, for example, whitening agents, humectants, antioxidants, oil-based ingredients, ultraviolet absorbers, surfactants, thickeners, alcohols, powder ingredients, colorants, aqueous ingredients, water, Various skin nutrients may be mentioned.

Dosage forms for cosmetics include, for example, solubilized systems, emulsified systems, powder systems, oil liquid systems, gel systems, ointment systems, aerosol systems, water-oil two-layer systems, water-oil-powder three-layer systems, etc. good. Examples of cosmetics include facial cleansers, lotions, emulsions, creams, gels, essences, serums, packs, masks, mist, basic cosmetics such as UV prevention cosmetics, foundations, lipsticks, blushers, eye shadows, eye liners, mascara, etc. Make-up cosmetics, facial wash, massage agent, cleansing agent, aftershave lotion, pre-shave lotion, shaving cream, body soap, soap, shampoo, conditioner, hair treatment, hair conditioner, hair tonic agent, hair mist, hair foam , hair liquid, hair gel, hair spray, hair restorer, antiperspirant, bath additive, mouth rinse, oral cosmetics, toothpaste, hand cream, hand soap, etc.

The content of honey in the cosmetic product is not particularly limited, and may be the same as the content of honey in the influenza virus killer, for example. Since honey has a direct killing effect on influenza viruses upon contact, cosmetics containing the influenza-killing agent (honey) of the present invention also have the effect of killing influenza viruses. The method for producing the above-mentioned cosmetics is not particularly limited, and any known method can be followed as appropriate. For example, a cosmetic used for the above purpose can be obtained by mixing the above honey with an intermediate product or a final product in the manufacturing process of cosmetics.

The disinfectant or cleaning agent contains honey in an effective amount and has the effect of eliminating, reducing or killing influenza viruses. The disinfectant or cleaning agent may be in any form such as solid, liquid, or paste, and is preferably in the form of a disinfectant sheet or cleaning sheet in the form of a wet tissue, a spray, or a paste. (water, hydrous alcohol, alcohol).

The effective amount of honey in the disinfectant or cleaning agent is not particularly limited and may be from 0.1% to 100% by weight, from 1% to 90% by weight, from 10% to 80% by weight, or from 25% by weight. % to 50% by weight. The method for producing the disinfectant or cleaning agent is not particularly limited, and any known method can be followed as appropriate.

Hereinafter, the present invention will be described in more detail based on Examples. However, the present invention is not limited to the following examples.

(Example 1 Examination of the virucidal effect of honey (1))
The test honeys were Manuka Honey MG250+ (Yamada Bee Farm Head Office), Manuka Honey MG350+ (Yamada Bee Farm Head Office), Honeydew Honey (Yamada Bee Farm Head Office), Ulmo Honey (Yamada Bee Farm Head Office), and Acacia Honey (Yamada Bee Farm Head Office). (Headquarters) and Bodaiju honey (Yamada Bee Farm Headquarters) were used. Add purified water to 1 g of each type of honey, dilute to 0.25 g/mL, and add 0 influenza virus solution (swine influenza virus H1N1 IOWA strain, 3.80 x 10 ⁹ copies/mL) to the resulting diluted solution. .1 mL was added thereto and allowed to stand at 25° C. for 25 hours to obtain a sensitizing solution. Purified water without honey was used as a control. The virus solution was added to the diluted honey solution, and the sensitization solution was collected after 2 hours, RNA was extracted by the porcelain bead method (Applied Biosystems MagMAX (trademark) CORE Nucleic Acid Purification Kit), and real-time PCR (Thermo Fisher Scientist). ific The virus amount (copies/mL) was calculated using StepOnePlus). From the obtained virus amount value, the virus reduction rate was calculated using the following formula. The higher the virus reduction rate, the higher the influenza virus killing effect. The results are shown in Table 1.
Virus reduction rate (%) = (1 - Virus amount after 2 hours in honey treatment area ÷ Virus amount after 2 hours in control area) x 100

From Table 1, it was found that all of the honeys examined exhibited virucidal effects. Among them, honeydew honey and ulmo honey had higher virucidal effects than manuka honey, acacia honey, and linden honey at the same concentration.

(Example 2 Examination of the virucidal effect of honey (2))
Rewarewa honey (Yamada Bee Farm Headquarters) was used as the test honey. Add purified water to 1 g of Rewarewa honey, dilute it to 0.25 g/mL, and add 0 influenza virus solution (swine influenza virus H1N1 IOWA strain, 3.80 x 10 ⁹ copies/mL) to the resulting diluted solution. .1 mL was added thereto and allowed to stand at 25° C. for 25 hours to obtain a sensitizing solution. Purified water without honey was used as a control. Add the virus solution to the diluted honey solution, collect the sensitization solution after 2 hours, extract RNA by the porcelain bead method as in Example 1, and calculate the amount of virus (copies/mL) by real-time PCR. did. As in Example 1, the virus reduction rate was calculated from the obtained virus amount values.

From Table 2, it was found that Rewarewa honey exhibits a virucidal effect. The virus reduction rate was higher than that of Manuka honey, Acacia honey, and Bodaiju honey at the same concentration in Example 1.

Claims (7)

A virus-killing influenza agent containing honey as an active ingredient. The influenza virus killing agent according to claim 1, wherein the honey is at least one selected from the group consisting of honeydew honey, ulmo honey, manuka honey, acacia honey, bodaiju honey, and rewarewa honey. The influenza virus killing agent according to claim 1 or 2, which reduces the number of influenza viruses by 50% or more. A food composition comprising the influenza virus killing agent according to claim 1 or 2. A cosmetic product comprising the influenza virus killing agent according to claim 1 or 2. A disinfectant comprising the influenza virus killing agent according to claim 1 or 2. A cleaning agent comprising the influenza virus killing agent according to claim 1 or 2.