JP2023544783A - 操作されたホスホペントムターゼ改変体酵素 - Google Patents

操作されたホスホペントムターゼ改変体酵素 Download PDF

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Publication number
JP2023544783A
JP2023544783A JP2023521079A JP2023521079A JP2023544783A JP 2023544783 A JP2023544783 A JP 2023544783A JP 2023521079 A JP2023521079 A JP 2023521079A JP 2023521079 A JP2023521079 A JP 2023521079A JP 2023544783 A JP2023544783 A JP 2023544783A
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JP
Japan
Prior art keywords
engineered
sequence
seq
phosphopentomutase
polypeptide
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Pending
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JP2023521079A
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English (en)
Japanese (ja)
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JP2023544783A5 (https=
Inventor
ジョナサン ブルーム，
サントシュ シヴァラマクリシュナン，
ジェシカ アンナ フルタク，
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コデクシス， インコーポレイテッド
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Application filed by コデクシス， インコーポレイテッド filed Critical コデクシス， インコーポレイテッド
Publication of JP2023544783A publication Critical patent/JP2023544783A/ja
Publication of JP2023544783A5 publication Critical patent/JP2023544783A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02007—Phosphopentomutase (5.4.2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2023521079A 2020-10-07 2021-10-05 操作されたホスホペントムターゼ改変体酵素 Pending JP2023544783A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063088556P 2020-10-07 2020-10-07
US63/088,556 2020-10-07
PCT/US2021/053626 WO2022076454A1 (en) 2020-10-07 2021-10-05 Engineered phosphopentomutase variant enzymes

Publications (2)

Publication Number Publication Date
JP2023544783A true JP2023544783A (ja) 2023-10-25
JP2023544783A5 JP2023544783A5 (https=) 2024-10-04

Family

ID=81125465

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023521079A Pending JP2023544783A (ja) 2020-10-07 2021-10-05 操作されたホスホペントムターゼ改変体酵素

Country Status (8)

Country Link
US (1) US20230374486A1 (https=)
EP (1) EP4225909A4 (https=)
JP (1) JP2023544783A (https=)
CN (1) CN116710558A (https=)
AU (1) AU2021358938A1 (https=)
CA (1) CA3196715A1 (https=)
IL (1) IL301857A (https=)
WO (1) WO2022076454A1 (https=)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020014046A1 (en) * 2018-07-09 2020-01-16 Codexis, Inc. Engineered phosphopentomutase variant enzymes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1858920T1 (sl) * 2005-02-18 2016-07-29 Glaxosmithkline Biologicals S.A. Proteini in nukleinske kisline iz escherichia coli, povezane z meningitisom/sepso

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020014046A1 (en) * 2018-07-09 2020-01-16 Codexis, Inc. Engineered phosphopentomutase variant enzymes

Also Published As

Publication number Publication date
US20230374486A1 (en) 2023-11-23
EP4225909A4 (en) 2025-02-12
CN116710558A (zh) 2023-09-05
AU2021358938A1 (en) 2023-04-27
WO2022076454A1 (en) 2022-04-14
CA3196715A1 (en) 2022-04-14
IL301857A (en) 2023-06-01
EP4225909A1 (en) 2023-08-16

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