AU2021358938A1 - Engineered phosphopentomutase variant enzymes - Google Patents

Engineered phosphopentomutase variant enzymes Download PDF

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Publication number
AU2021358938A1
AU2021358938A1 AU2021358938A AU2021358938A AU2021358938A1 AU 2021358938 A1 AU2021358938 A1 AU 2021358938A1 AU 2021358938 A AU2021358938 A AU 2021358938A AU 2021358938 A AU2021358938 A AU 2021358938A AU 2021358938 A1 AU2021358938 A1 AU 2021358938A1
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AU
Australia
Prior art keywords
sequence
seq
phosphopentomutase
engineered
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2021358938A
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English (en)
Inventor
Jessica Anna HURTAK
Santhosh Sivaramakrishnan
Jonathan VROOM
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
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Codexis Inc
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Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of AU2021358938A1 publication Critical patent/AU2021358938A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y504/00Intramolecular transferases (5.4)
    • C12Y504/02Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02007Phosphopentomutase (5.4.2.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2021358938A 2020-10-07 2021-10-05 Engineered phosphopentomutase variant enzymes Abandoned AU2021358938A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063088556P 2020-10-07 2020-10-07
US63/088,556 2020-10-07
PCT/US2021/053626 WO2022076454A1 (en) 2020-10-07 2021-10-05 Engineered phosphopentomutase variant enzymes

Publications (1)

Publication Number Publication Date
AU2021358938A1 true AU2021358938A1 (en) 2023-04-27

Family

ID=81125465

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021358938A Abandoned AU2021358938A1 (en) 2020-10-07 2021-10-05 Engineered phosphopentomutase variant enzymes

Country Status (8)

Country Link
US (1) US20230374486A1 (https=)
EP (1) EP4225909A4 (https=)
JP (1) JP2023544783A (https=)
CN (1) CN116710558A (https=)
AU (1) AU2021358938A1 (https=)
CA (1) CA3196715A1 (https=)
IL (1) IL301857A (https=)
WO (1) WO2022076454A1 (https=)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1858920T1 (sl) * 2005-02-18 2016-07-29 Glaxosmithkline Biologicals S.A. Proteini in nukleinske kisline iz escherichia coli, povezane z meningitisom/sepso
EP3821026A4 (en) * 2018-07-09 2022-06-01 Codexis, Inc. MANIPULATED ENZYME VARIANTS OF PHOSPHOPENTOMUTASE

Also Published As

Publication number Publication date
JP2023544783A (ja) 2023-10-25
US20230374486A1 (en) 2023-11-23
EP4225909A4 (en) 2025-02-12
CN116710558A (zh) 2023-09-05
WO2022076454A1 (en) 2022-04-14
CA3196715A1 (en) 2022-04-14
IL301857A (en) 2023-06-01
EP4225909A1 (en) 2023-08-16

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