JP2023537007A - 非天然アミノ酸の部位特異的組み込みのための安定な細胞株 - Google Patents

非天然アミノ酸の部位特異的組み込みのための安定な細胞株 Download PDF

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JP2023537007A
JP2023537007A JP2023507992A JP2023507992A JP2023537007A JP 2023537007 A JP2023537007 A JP 2023537007A JP 2023507992 A JP2023507992 A JP 2023507992A JP 2023507992 A JP2023507992 A JP 2023507992A JP 2023537007 A JP2023537007 A JP 2023537007A
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trna
trna synthetase
mutein
cell
amino acid
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JP2023537007A5 (enExample
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セバスチャン イタリア，ジェームズ
リー，ジー
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ブリックバイオ，インコーポレイテッド
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Publication of JP2023537007A publication Critical patent/JP2023537007A/ja
Publication of JP2023537007A5 publication Critical patent/JP2023537007A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01002—Tryptophan-tRNA ligase (6.1.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01004—Leucine--tRNA ligase (6.1.1.4)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Superconductors And Manufacturing Methods Therefor (AREA)
  • Peptides Or Proteins (AREA)
JP2023507992A 2020-08-07 2021-08-06 非天然アミノ酸の部位特異的組み込みのための安定な細胞株 Pending JP2023537007A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063063065P 2020-08-07 2020-08-07
US63/063,065 2020-08-07
US202163210593P 2021-06-15 2021-06-15
US63/210,593 2021-06-15
PCT/US2021/045088 WO2022032186A2 (en) 2020-08-07 2021-08-06 Stable cell lines for site-specific incorporation of unnatural amino acids

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JP2023537007A true JP2023537007A (ja) 2023-08-30
JP2023537007A5 JP2023537007A5 (enExample) 2024-08-14

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JP2023507992A Pending JP2023537007A (ja) 2020-08-07 2021-08-06 非天然アミノ酸の部位特異的組み込みのための安定な細胞株

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US (1) US20230313129A1 (enExample)
EP (1) EP4192943A4 (enExample)
JP (1) JP2023537007A (enExample)
AU (1) AU2021320894A1 (enExample)
CA (1) CA3188528A1 (enExample)
WO (1) WO2022032186A2 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114908066B (zh) * 2022-05-17 2024-01-23 杭州嵌化合生医药科技有限公司 一种正交翻译系统及其在再分配密码子恢复ptc疾病中功能蛋白表达方面的应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130244245A1 (en) * 2004-10-27 2013-09-19 The Scripps Research Institute Orthogonal Translation Components for the in Vivo Incorporation of Unnatural Amino Acids
CN109295100A (zh) * 2017-07-25 2019-02-01 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN110835633A (zh) * 2018-08-13 2020-02-25 北京大学 利用优化的基因密码子扩展系统制备ptc稳定细胞系及应用
JP2022507806A (ja) * 2018-12-04 2022-01-18 エスエヌシー ラブ ウェブ接近性および開放性評価方法および装置

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090181858A1 (en) * 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
US20220325269A1 (en) * 2019-08-08 2022-10-13 Brickbio, Inc. Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130244245A1 (en) * 2004-10-27 2013-09-19 The Scripps Research Institute Orthogonal Translation Components for the in Vivo Incorporation of Unnatural Amino Acids
CN109295100A (zh) * 2017-07-25 2019-02-01 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN110835633A (zh) * 2018-08-13 2020-02-25 北京大学 利用优化的基因密码子扩展系统制备ptc稳定细胞系及应用
JP2022507806A (ja) * 2018-12-04 2022-01-18 エスエヌシー ラブ ウェブ接近性および開放性評価方法および装置

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AI, HUI‐WANG ET AL., ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 49, no. 5, JPN6025037790, 2010, pages 935 - 937, ISSN: 0005685617 *
ELSSSER, SIMON J., METHODS IN MOLECULAR BIOLOGY, JPN6025037788, 2018, pages 237 - 245, ISSN: 0005685619 *
SHEN, BIN ET AL., STEM CELLS, vol. 29, no. 8, JPN6025037789, 2011, pages 1231 - 1240, ISSN: 0005685618 *
ZHENG, YUNAN ET AL., BIOCHEMISTRY, vol. 57, no. 4, JPN6025037791, 2017, pages 441 - 445, ISSN: 0005685616 *

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Publication number Publication date
EP4192943A2 (en) 2023-06-14
EP4192943A4 (en) 2025-02-12
CA3188528A1 (en) 2022-02-10
US20230313129A1 (en) 2023-10-05
WO2022032186A3 (en) 2022-04-07
WO2022032186A2 (en) 2022-02-10
AU2021320894A1 (en) 2023-03-02

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