CA3188528A1 - Stable cell lines for site-specific incorporation of unnatural amino acids - Google Patents
Stable cell lines for site-specific incorporation of unnatural amino acidsInfo
- Publication number
- CA3188528A1 CA3188528A1 CA3188528A CA3188528A CA3188528A1 CA 3188528 A1 CA3188528 A1 CA 3188528A1 CA 3188528 A CA3188528 A CA 3188528A CA 3188528 A CA3188528 A CA 3188528A CA 3188528 A1 CA3188528 A1 CA 3188528A1
- Authority
- CA
- Canada
- Prior art keywords
- trna
- trna synthetase
- cell
- mutein
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
- C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
- C12Y601/01002—Tryptophan-tRNA ligase (6.1.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
- C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
- C12Y601/01004—Leucine--tRNA ligase (6.1.1.4)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Superconductors And Manufacturing Methods Therefor (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063063065P | 2020-08-07 | 2020-08-07 | |
| US63/063,065 | 2020-08-07 | ||
| US202163210593P | 2021-06-15 | 2021-06-15 | |
| US63/210,593 | 2021-06-15 | ||
| PCT/US2021/045088 WO2022032186A2 (en) | 2020-08-07 | 2021-08-06 | Stable cell lines for site-specific incorporation of unnatural amino acids |
Publications (1)
| Publication Number | Publication Date |
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| CA3188528A1 true CA3188528A1 (en) | 2022-02-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CA3188528A Pending CA3188528A1 (en) | 2020-08-07 | 2021-08-06 | Stable cell lines for site-specific incorporation of unnatural amino acids |
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| US (1) | US20230313129A1 (enExample) |
| EP (1) | EP4192943A4 (enExample) |
| JP (1) | JP2023537007A (enExample) |
| AU (1) | AU2021320894A1 (enExample) |
| CA (1) | CA3188528A1 (enExample) |
| WO (1) | WO2022032186A2 (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114908066B (zh) * | 2022-05-17 | 2024-01-23 | 杭州嵌化合生医药科技有限公司 | 一种正交翻译系统及其在再分配密码子恢复ptc疾病中功能蛋白表达方面的应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20090181858A1 (en) * | 2004-09-21 | 2009-07-16 | The Scripps Research Institute | Adding photoregulated amino acids to the genetic code |
| CN101048506B (zh) * | 2004-10-27 | 2013-06-26 | 斯克利普斯研究院 | 体内掺入非天然氨基酸的正交翻译组分 |
| CN109295100A (zh) * | 2017-07-25 | 2019-02-01 | 北京大学 | 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建 |
| CN110835633B (zh) * | 2018-08-13 | 2021-10-01 | 北京大学 | 利用优化的基因密码子扩展系统制备ptc稳定细胞系及应用 |
| KR101950208B1 (ko) * | 2018-12-04 | 2019-02-22 | (주)에스앤씨랩 | 웹 접근성 및 개방성 평가 방법 및 장치 |
| US20220325269A1 (en) * | 2019-08-08 | 2022-10-13 | Brickbio, Inc. | Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids |
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2021
- 2021-08-06 EP EP21853196.0A patent/EP4192943A4/en not_active Withdrawn
- 2021-08-06 AU AU2021320894A patent/AU2021320894A1/en not_active Abandoned
- 2021-08-06 US US18/040,933 patent/US20230313129A1/en active Pending
- 2021-08-06 WO PCT/US2021/045088 patent/WO2022032186A2/en not_active Ceased
- 2021-08-06 CA CA3188528A patent/CA3188528A1/en active Pending
- 2021-08-06 JP JP2023507992A patent/JP2023537007A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4192943A2 (en) | 2023-06-14 |
| EP4192943A4 (en) | 2025-02-12 |
| US20230313129A1 (en) | 2023-10-05 |
| WO2022032186A3 (en) | 2022-04-07 |
| JP2023537007A (ja) | 2023-08-30 |
| WO2022032186A2 (en) | 2022-02-10 |
| AU2021320894A1 (en) | 2023-03-02 |
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