JP2023535407A - 「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法 - Google Patents

「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法 Download PDF

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JP2023535407A
JP2023535407A JP2023504254A JP2023504254A JP2023535407A JP 2023535407 A JP2023535407 A JP 2023535407A JP 2023504254 A JP2023504254 A JP 2023504254A JP 2023504254 A JP2023504254 A JP 2023504254A JP 2023535407 A JP2023535407 A JP 2023535407A
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tag
sequence
sequences
primer
seq
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JP2023535407A5 (https=
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マクニール，マシュー
ターク，ロルフ
レティグ，ギャレット
ブラック，エレン
スン，ヨンミン
セーラー，クリス
ワン，ユー
ガンダーソン，キース
キニー，カイル
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インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド
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Publication of JP2023535407A publication Critical patent/JP2023535407A/ja
Publication of JP2023535407A5 publication Critical patent/JP2023535407A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2023504254A 2020-07-23 2021-07-22 「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法 Pending JP2023535407A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025279789A JP2026048971A (ja) 2020-07-23 2025-12-24 「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063055460P 2020-07-23 2020-07-23
US63/055,460 2020-07-23
PCT/US2021/042733 WO2022020567A2 (en) 2020-07-23 2021-07-22 Methods for nomination of nuclease on-/off-target editing locations, designated "ctl-seq" (crispr tag linear-seq)

Related Child Applications (1)

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JP2025279789A Division JP2026048971A (ja) 2020-07-23 2025-12-24 「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法

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JP2023535407A true JP2023535407A (ja) 2023-08-17
JP2023535407A5 JP2023535407A5 (https=) 2024-03-26

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JP2023504254A Pending JP2023535407A (ja) 2020-07-23 2021-07-22 「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法
JP2025279789A Pending JP2026048971A (ja) 2020-07-23 2025-12-24 「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法

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JP2025279789A Pending JP2026048971A (ja) 2020-07-23 2025-12-24 「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法

Country Status (8)

Country Link
US (2) US20220025365A1 (https=)
EP (1) EP4185708A2 (https=)
JP (2) JP2023535407A (https=)
KR (1) KR20230040370A (https=)
CN (1) CN116194593A (https=)
AU (1) AU2021311713A1 (https=)
CA (1) CA3185571A1 (https=)
WO (1) WO2022020567A2 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202013141D0 (en) 2020-08-21 2020-10-07 Univ College Cardiff Consultants Ltd A screening method for the detection of DNA damage
WO2025151661A1 (en) * 2024-01-10 2025-07-17 Sequre Dx, Inc. Methods for single-ended oligonucleotide enrichment and sequencing
WO2026039499A1 (en) 2024-08-14 2026-02-19 Integrated Dna Technologies, Inc. Adaptered-tag blocking oligonucleotides

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120283110A1 (en) * 2011-04-21 2012-11-08 Jay Shendure Methods for retrieval of sequence-verified dna constructs
JP2015536156A (ja) * 2012-12-10 2015-12-21 レゾリューション バイオサイエンス， インコーポレイテッド 標的化ゲノム解析のための方法
WO2016081798A1 (en) * 2014-11-20 2016-05-26 Children's Medical Center Corporation Methods relating to the detection of recurrent and non-specific double strand breaks in the genome
JP2017519508A (ja) * 2014-06-23 2017-07-20 ザ ジェネラル ホスピタル コーポレイション シークエンシングによって評価されるゲノムワイドでバイアスのないＤＳＢの同定（ＧＵＩＤＥ−Ｓｅｑ）
JP2020511966A (ja) * 2017-03-23 2020-04-23 ユニヴァーシティ オブ ワシントン エラーが訂正された核酸配列決定への適用を伴う標的化核酸配列濃縮のための方法
JP2020530290A (ja) * 2017-08-07 2020-10-22 ザ ジョンズ ホプキンス ユニバーシティ がんを評価及び治療するための方法及び物質

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2906365A1 (en) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
WO2016030899A1 (en) * 2014-08-28 2016-03-03 Yeda Research And Development Co. Ltd. Methods of treating amyotrophic lateral scleroses
WO2019110067A1 (en) * 2017-12-07 2019-06-13 Aarhus Universitet Hybrid nanoparticle

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120283110A1 (en) * 2011-04-21 2012-11-08 Jay Shendure Methods for retrieval of sequence-verified dna constructs
JP2015536156A (ja) * 2012-12-10 2015-12-21 レゾリューション バイオサイエンス， インコーポレイテッド 標的化ゲノム解析のための方法
JP2017519508A (ja) * 2014-06-23 2017-07-20 ザ ジェネラル ホスピタル コーポレイション シークエンシングによって評価されるゲノムワイドでバイアスのないＤＳＢの同定（ＧＵＩＤＥ−Ｓｅｑ）
WO2016081798A1 (en) * 2014-11-20 2016-05-26 Children's Medical Center Corporation Methods relating to the detection of recurrent and non-specific double strand breaks in the genome
JP2020511966A (ja) * 2017-03-23 2020-04-23 ユニヴァーシティ オブ ワシントン エラーが訂正された核酸配列決定への適用を伴う標的化核酸配列濃縮のための方法
JP2020530290A (ja) * 2017-08-07 2020-10-22 ザ ジョンズ ホプキンス ユニバーシティ がんを評価及び治療するための方法及び物質

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Publication number Publication date
WO2022020567A3 (en) 2022-03-10
JP2026048971A (ja) 2026-03-17
CA3185571A1 (en) 2022-01-27
CN116194593A (zh) 2023-05-30
US20220025365A1 (en) 2022-01-27
KR20230040370A (ko) 2023-03-22
EP4185708A2 (en) 2023-05-31
US20250257352A1 (en) 2025-08-14
AU2021311713A1 (en) 2023-03-09
WO2022020567A2 (en) 2022-01-27

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