JP2023530098A - 間葉系前駆細胞又は幹細胞の3d培養 - Google Patents

間葉系前駆細胞又は幹細胞の3d培養 Download PDF

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JP2023530098A
JP2023530098A JP2022576173A JP2022576173A JP2023530098A JP 2023530098 A JP2023530098 A JP 2023530098A JP 2022576173 A JP2022576173 A JP 2022576173A JP 2022576173 A JP2022576173 A JP 2022576173A JP 2023530098 A JP2023530098 A JP 2023530098A
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cells
stem cells
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mesenchymal progenitor
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ポール・シモンズ
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メゾブラスト・インターナショナル・エスアーエールエル
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JP2022576173A 2020-06-11 2021-06-09 間葉系前駆細胞又は幹細胞の3d培養 Pending JP2023530098A (ja)

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AU2020901931A AU2020901931A0 (en) 2020-06-11 3D culture of mesenchymal lineage precursor or stem cells
AU2020901931 2020-06-11
PCT/IB2021/055055 WO2021250583A2 (en) 2020-06-11 2021-06-09 3d culture of mesenchymal lineage precursor or stem cells

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JP2023530098A true JP2023530098A (ja) 2023-07-13
JP2023530098A5 JP2023530098A5 (https=) 2024-06-14

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US (1) US20230220349A1 (https=)
EP (1) EP4165166A2 (https=)
JP (1) JP2023530098A (https=)
KR (1) KR20230022242A (https=)
CN (1) CN115997005A (https=)
AU (1) AU2021289205A1 (https=)
BR (1) BR112022024866A2 (https=)
CA (1) CA3181611A1 (https=)
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Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
US20120087868A1 (en) 2010-10-08 2012-04-12 Gabriele Todd Nanoparticle-loaded cells
SG11202004680UA (en) 2017-11-22 2020-06-29 Mesoblast Int Sarl Cellular compositions and methods of treatment i
CN121219346A (zh) * 2023-05-30 2025-12-26 旭化成株式会社 纤维素多孔粒子及其制造方法
WO2025196663A1 (en) * 2024-03-19 2025-09-25 Mesoblast International Sarl Potency assay and manufacturing method ii

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011500118A (ja) * 2007-10-11 2011-01-06 アンセルム(アンスチチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル) 組織工学、細胞培養、および細胞送達用の多孔質足場の調製方法
JP2016521124A (ja) * 2013-04-10 2016-07-21 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ 幹細胞培養のためのポリカプロラクトン・マイクロキャリアーおよびその製造
JP2016523086A (ja) * 2013-06-24 2016-08-08 コーニング インコーポレイテッド 細胞培養物品およびその方法
JP2018506986A (ja) * 2015-03-04 2018-03-15 メゾブラスト・インターナショナル・エスアーエールエル 間葉系幹細胞の細胞培養法
WO2020086889A1 (en) * 2018-10-24 2020-04-30 Hebecell Corporation Methods and systems for manufacturing hematopoietic lineage cells
WO2020100481A1 (ja) * 2018-11-15 2020-05-22 Jsr株式会社 脳オルガノイドの製造方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5902741A (en) 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5629186A (en) 1994-04-28 1997-05-13 Lockheed Martin Corporation Porous matrix and method of its production
US6132463A (en) 1995-05-19 2000-10-17 Etex Corporation Cell seeding of ceramic compositions
US6251295B1 (en) 1998-01-08 2001-06-26 Nexell Therapeutics Inc. Method for recirculation washing of blood cells
DE69903800T2 (de) 1998-03-18 2003-10-02 Massachusetts Institute Of Technology, Cambridge Vaskularisierte perfundierte anordnungen für mikrogewebe und mikroorgane
BR0009403A (pt) 1999-02-04 2001-11-27 Technion Res & Dev Foundation Método de expansão/conservação das células detronco hemopoiéticas indiferenciadas ou dascélulas progenitoras, método de preparação deum meio condicionado de célula estomacal útil naexpansão/conservação das células de troncohemopoiéticas indiferenciadas ou das célulasprogenitoras, método de transplante de célulasde tronco hemopoiéticas indiferenciadas ou decélulas progenitoras em um recipiente, tampão debiorreator e biorreator
JP2012520672A (ja) * 2009-03-20 2012-09-10 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ マイクロキャリアー上の多能性(pluripotent)および多分化能(multipotent)細胞の培養
TWI741980B (zh) * 2015-04-07 2021-10-11 大陸商四川藍光英諾生物科技股份有限公司 一種生物磚及其用途
WO2017132358A1 (en) * 2016-01-26 2017-08-03 Kansas State University Research Foundation Methods for isolation and expansion of umbilical cord mesenchymal stem cells
SG11201909856XA (en) * 2017-05-04 2019-11-28 Mesoblast Int Sarl Mesenchymal lineage precursor or stem cells with enhanced immunosuppression
CN108715833B (zh) * 2018-06-01 2021-09-14 天晴干细胞股份有限公司 一种负载血小板裂解液的微球制备方法
KR20200015174A (ko) * 2018-08-03 2020-02-12 이동희 3차원 세포 배양 지지체 및 이를 포함하는 줄기세포 배양 조성물
CN112522191B (zh) * 2020-12-18 2023-05-12 云南中科灵长类生物医学重点实验室 一种间充质干细胞的培养方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011500118A (ja) * 2007-10-11 2011-01-06 アンセルム(アンスチチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル) 組織工学、細胞培養、および細胞送達用の多孔質足場の調製方法
JP2016521124A (ja) * 2013-04-10 2016-07-21 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ 幹細胞培養のためのポリカプロラクトン・マイクロキャリアーおよびその製造
JP2016523086A (ja) * 2013-06-24 2016-08-08 コーニング インコーポレイテッド 細胞培養物品およびその方法
JP2018506986A (ja) * 2015-03-04 2018-03-15 メゾブラスト・インターナショナル・エスアーエールエル 間葉系幹細胞の細胞培養法
WO2020086889A1 (en) * 2018-10-24 2020-04-30 Hebecell Corporation Methods and systems for manufacturing hematopoietic lineage cells
WO2020100481A1 (ja) * 2018-11-15 2020-05-22 Jsr株式会社 脳オルガノイドの製造方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHASE LG ET AL.: ""A novel serum-free medium for the expansion of human mesenchymal stem cells"", STEM CELL RESEARCH & THERAPY [ONLINE], vol. Volume 1, article number 8, JPN6025026604, 2 April 2010 (2010-04-02), pages 1 - 11, ISSN: 0005828464 *

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WO2021250583A3 (en) 2022-02-24
US20230220349A1 (en) 2023-07-13
WO2021250583A2 (en) 2021-12-16
CA3181611A1 (en) 2021-12-16
EP4165166A2 (en) 2023-04-19
KR20230022242A (ko) 2023-02-14
CN115997005A (zh) 2023-04-21
BR112022024866A2 (pt) 2023-02-14
AU2021289205A1 (en) 2023-02-02

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