BR112022024866A2 - Cultura 3d de células precursoras da linhagem mesenquimal ou células-tronco - Google Patents
Cultura 3d de células precursoras da linhagem mesenquimal ou células-troncoInfo
- Publication number
- BR112022024866A2 BR112022024866A2 BR112022024866A BR112022024866A BR112022024866A2 BR 112022024866 A2 BR112022024866 A2 BR 112022024866A2 BR 112022024866 A BR112022024866 A BR 112022024866A BR 112022024866 A BR112022024866 A BR 112022024866A BR 112022024866 A2 BR112022024866 A2 BR 112022024866A2
- Authority
- BR
- Brazil
- Prior art keywords
- culture
- cells
- mesenchymal lineage
- lineage precursor
- stem cells
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 title abstract 3
- 210000000130 stem cell Anatomy 0.000 title abstract 3
- 239000002243 precursor Substances 0.000 title abstract 2
- 238000004113 cell culture Methods 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/80—Undefined extracts from animals
- C12N2500/84—Undefined extracts from animals from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/92—Medium free of human- or animal-derived components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
- C12N2502/115—Platelets, megakaryocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020901931A AU2020901931A0 (en) | 2020-06-11 | 3D culture of mesenchymal lineage precursor or stem cells | |
| PCT/IB2021/055055 WO2021250583A2 (en) | 2020-06-11 | 2021-06-09 | 3d culture of mesenchymal lineage precursor or stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112022024866A2 true BR112022024866A2 (pt) | 2023-02-14 |
Family
ID=76641730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112022024866A BR112022024866A2 (pt) | 2020-06-11 | 2021-06-09 | Cultura 3d de células precursoras da linhagem mesenquimal ou células-tronco |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20230220349A1 (https=) |
| EP (1) | EP4165166A2 (https=) |
| JP (1) | JP2023530098A (https=) |
| KR (1) | KR20230022242A (https=) |
| CN (1) | CN115997005A (https=) |
| AU (1) | AU2021289205A1 (https=) |
| BR (1) | BR112022024866A2 (https=) |
| CA (1) | CA3181611A1 (https=) |
| WO (1) | WO2021250583A2 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120087868A1 (en) | 2010-10-08 | 2012-04-12 | Gabriele Todd | Nanoparticle-loaded cells |
| SG11202004680UA (en) | 2017-11-22 | 2020-06-29 | Mesoblast Int Sarl | Cellular compositions and methods of treatment i |
| CN121219346A (zh) * | 2023-05-30 | 2025-12-26 | 旭化成株式会社 | 纤维素多孔粒子及其制造方法 |
| WO2025196663A1 (en) * | 2024-03-19 | 2025-09-25 | Mesoblast International Sarl | Potency assay and manufacturing method ii |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5902741A (en) | 1986-04-18 | 1999-05-11 | Advanced Tissue Sciences, Inc. | Three-dimensional cartilage cultures |
| US5486359A (en) | 1990-11-16 | 1996-01-23 | Osiris Therapeutics, Inc. | Human mesenchymal stem cells |
| US5629186A (en) | 1994-04-28 | 1997-05-13 | Lockheed Martin Corporation | Porous matrix and method of its production |
| US6132463A (en) | 1995-05-19 | 2000-10-17 | Etex Corporation | Cell seeding of ceramic compositions |
| US6251295B1 (en) | 1998-01-08 | 2001-06-26 | Nexell Therapeutics Inc. | Method for recirculation washing of blood cells |
| DE69903800T2 (de) | 1998-03-18 | 2003-10-02 | Massachusetts Institute Of Technology, Cambridge | Vaskularisierte perfundierte anordnungen für mikrogewebe und mikroorgane |
| BR0009403A (pt) | 1999-02-04 | 2001-11-27 | Technion Res & Dev Foundation | Método de expansão/conservação das células detronco hemopoiéticas indiferenciadas ou dascélulas progenitoras, método de preparação deum meio condicionado de célula estomacal útil naexpansão/conservação das células de troncohemopoiéticas indiferenciadas ou das célulasprogenitoras, método de transplante de célulasde tronco hemopoiéticas indiferenciadas ou decélulas progenitoras em um recipiente, tampão debiorreator e biorreator |
| WO2009047346A1 (en) * | 2007-10-11 | 2009-04-16 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Method for preparing porous scaffold for tissue engineering, cell culture and cell delivery |
| JP2012520672A (ja) * | 2009-03-20 | 2012-09-10 | エージェンシー フォー サイエンス，テクノロジー アンド リサーチ | マイクロキャリアー上の多能性（ｐｌｕｒｉｐｏｔｅｎｔ）および多分化能（ｍｕｌｔｉｐｏｔｅｎｔ）細胞の培養 |
| WO2014168585A1 (en) * | 2013-04-10 | 2014-10-16 | Agency For Science, Technology And Research | Polycaprolactone microcarriers for stem cell culture and fabrication thereof |
| EP3013940B1 (en) * | 2013-06-24 | 2017-04-12 | Corning Incorporated | Cell culture article and methods thereof |
| EP4008775A1 (en) * | 2015-03-04 | 2022-06-08 | Mesoblast International Sàrl | Cell culture method for mesenchymal stem cells |
| TWI741980B (zh) * | 2015-04-07 | 2021-10-11 | 大陸商四川藍光英諾生物科技股份有限公司 | 一種生物磚及其用途 |
| WO2017132358A1 (en) * | 2016-01-26 | 2017-08-03 | Kansas State University Research Foundation | Methods for isolation and expansion of umbilical cord mesenchymal stem cells |
| SG11201909856XA (en) * | 2017-05-04 | 2019-11-28 | Mesoblast Int Sarl | Mesenchymal lineage precursor or stem cells with enhanced immunosuppression |
| CN108715833B (zh) * | 2018-06-01 | 2021-09-14 | 天晴干细胞股份有限公司 | 一种负载血小板裂解液的微球制备方法 |
| KR20200015174A (ko) * | 2018-08-03 | 2020-02-12 | 이동희 | 3차원 세포 배양 지지체 및 이를 포함하는 줄기세포 배양 조성물 |
| AU2019368313A1 (en) * | 2018-10-24 | 2021-05-20 | Hebecell Corporation | Methods and systems for manufacturing hematopoietic lineage cells |
| WO2020100481A1 (ja) * | 2018-11-15 | 2020-05-22 | Jsr株式会社 | 脳オルガノイドの製造方法 |
| CN112522191B (zh) * | 2020-12-18 | 2023-05-12 | 云南中科灵长类生物医学重点实验室 | 一种间充质干细胞的培养方法 |
-
2021
- 2021-06-09 JP JP2022576173A patent/JP2023530098A/ja active Pending
- 2021-06-09 CA CA3181611A patent/CA3181611A1/en active Pending
- 2021-06-09 CN CN202180047714.6A patent/CN115997005A/zh active Pending
- 2021-06-09 AU AU2021289205A patent/AU2021289205A1/en active Pending
- 2021-06-09 WO PCT/IB2021/055055 patent/WO2021250583A2/en not_active Ceased
- 2021-06-09 EP EP21735402.6A patent/EP4165166A2/en active Pending
- 2021-06-09 US US18/001,462 patent/US20230220349A1/en active Pending
- 2021-06-09 KR KR1020237001093A patent/KR20230022242A/ko active Pending
- 2021-06-09 BR BR112022024866A patent/BR112022024866A2/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2023530098A (ja) | 2023-07-13 |
| WO2021250583A3 (en) | 2022-02-24 |
| US20230220349A1 (en) | 2023-07-13 |
| WO2021250583A2 (en) | 2021-12-16 |
| CA3181611A1 (en) | 2021-12-16 |
| EP4165166A2 (en) | 2023-04-19 |
| KR20230022242A (ko) | 2023-02-14 |
| CN115997005A (zh) | 2023-04-21 |
| AU2021289205A1 (en) | 2023-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112022024866A2 (pt) | Cultura 3d de células precursoras da linhagem mesenquimal ou células-tronco | |
| Li et al. | A review of green biohydrogen production using anoxygenic photosynthetic bacteria for hydrogen economy: challenges and opportunities | |
| MX356756B (es) | Células madre pluripotentes en microportadores. | |
| SG196836A1 (en) | Cancer stem cell population and method for production thereof | |
| MX353489B (es) | Metodo para producir eritrocitos sin celulas alimentadoras. | |
| SG178914A1 (en) | Stem cell conditioned medium compositions | |
| BR112022017216A2 (pt) | Método para produzir células exterminadoras naturais a partir de células-tronco pluripotentes | |
| CA2830080C (en) | Generation of nk cells and nk-cell progenitors | |
| NZ703529A (en) | Novel biocatalyst compositions and processes for use | |
| MX382080B (es) | Método para cultivar células eucariónticas. | |
| MX345399B (es) | Metodo de cultivo de celulas animales. | |
| BR112013002811A8 (pt) | meios básicos simplificados para cultura celular pluripotente de humano | |
| BR112015019950A2 (pt) | Método para gerar linhagem de células-tronco embrionárias (es) de rato, e, cultura in vitro | |
| EP2408903A4 (en) | PLURIPOTENTE AND MULTIPOTENTE CELL CULTURE ON MICROWAVES | |
| NZ734670A (en) | Cells for producing recombinant iduronate-2-sulfatase | |
| MX2010002426A (es) | Agente para promover la adhesion de las celulas del endotelio corneal. | |
| NZ603546A (en) | Yeast production culture for the production of butanol | |
| NZ609731A (en) | Compositions of adult organ stem cells and uses thereof | |
| NZ709124A (en) | Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same | |
| WO2014087244A3 (en) | Methods of producing rpe cells | |
| PH12014501019A1 (en) | Kit comprising serum replacement and labile factors | |
| WO2012131733A3 (en) | Media compositions for enriching cultures of stem cells | |
| BR112022025348A2 (pt) | Sistemas e métodos para a conversão de célula | |
| MX343113B (es) | Medios de cultivo de celulas granulados en seco. | |
| GB2539174A (en) | Bioreactor system for stem cell expansion |