KR20230022242A - 중간엽 계통 전구세포 또는 줄기세포의 3차원 배양 - Google Patents

중간엽 계통 전구세포 또는 줄기세포의 3차원 배양 Download PDF

Info

Publication number
KR20230022242A
KR20230022242A KR1020237001093A KR20237001093A KR20230022242A KR 20230022242 A KR20230022242 A KR 20230022242A KR 1020237001093 A KR1020237001093 A KR 1020237001093A KR 20237001093 A KR20237001093 A KR 20237001093A KR 20230022242 A KR20230022242 A KR 20230022242A
Authority
KR
South Korea
Prior art keywords
cells
stem cells
culture medium
cell
another embodiment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020237001093A
Other languages
English (en)
Korean (ko)
Inventor
폴 시몬스
Original Assignee
메조블라스트 인터내셔널 에스에이알엘
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2020901931A external-priority patent/AU2020901931A0/en
Application filed by 메조블라스트 인터내셔널 에스에이알엘 filed Critical 메조블라스트 인터내셔널 에스에이알엘
Publication of KR20230022242A publication Critical patent/KR20230022242A/ko
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/70Undefined extracts
    • C12N2500/80Undefined extracts from animals
    • C12N2500/84Undefined extracts from animals from mammals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92Medium free of human- or animal-derived components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/11Epidermal growth factor [EGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/135Platelet-derived growth factor [PDGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/11Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115Platelets, megakaryocytes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00Microcarriers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/52Fibronectin; Laminin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/54Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
KR1020237001093A 2020-06-11 2021-06-09 중간엽 계통 전구세포 또는 줄기세포의 3차원 배양 Pending KR20230022242A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2020901931A AU2020901931A0 (en) 2020-06-11 3D culture of mesenchymal lineage precursor or stem cells
AU2020901931 2020-06-11
PCT/IB2021/055055 WO2021250583A2 (en) 2020-06-11 2021-06-09 3d culture of mesenchymal lineage precursor or stem cells

Publications (1)

Publication Number Publication Date
KR20230022242A true KR20230022242A (ko) 2023-02-14

Family

ID=76641730

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020237001093A Pending KR20230022242A (ko) 2020-06-11 2021-06-09 중간엽 계통 전구세포 또는 줄기세포의 3차원 배양

Country Status (9)

Country Link
US (1) US20230220349A1 (https=)
EP (1) EP4165166A2 (https=)
JP (1) JP2023530098A (https=)
KR (1) KR20230022242A (https=)
CN (1) CN115997005A (https=)
AU (1) AU2021289205A1 (https=)
BR (1) BR112022024866A2 (https=)
CA (1) CA3181611A1 (https=)
WO (1) WO2021250583A2 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120087868A1 (en) 2010-10-08 2012-04-12 Gabriele Todd Nanoparticle-loaded cells
SG11202004680UA (en) 2017-11-22 2020-06-29 Mesoblast Int Sarl Cellular compositions and methods of treatment i
CN121219346A (zh) * 2023-05-30 2025-12-26 旭化成株式会社 纤维素多孔粒子及其制造方法
WO2025196663A1 (en) * 2024-03-19 2025-09-25 Mesoblast International Sarl Potency assay and manufacturing method ii

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5902741A (en) 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5629186A (en) 1994-04-28 1997-05-13 Lockheed Martin Corporation Porous matrix and method of its production
US6132463A (en) 1995-05-19 2000-10-17 Etex Corporation Cell seeding of ceramic compositions
US6251295B1 (en) 1998-01-08 2001-06-26 Nexell Therapeutics Inc. Method for recirculation washing of blood cells
DE69903800T2 (de) 1998-03-18 2003-10-02 Massachusetts Institute Of Technology, Cambridge Vaskularisierte perfundierte anordnungen für mikrogewebe und mikroorgane
BR0009403A (pt) 1999-02-04 2001-11-27 Technion Res & Dev Foundation Método de expansão/conservação das células detronco hemopoiéticas indiferenciadas ou dascélulas progenitoras, método de preparação deum meio condicionado de célula estomacal útil naexpansão/conservação das células de troncohemopoiéticas indiferenciadas ou das célulasprogenitoras, método de transplante de célulasde tronco hemopoiéticas indiferenciadas ou decélulas progenitoras em um recipiente, tampão debiorreator e biorreator
WO2009047346A1 (en) * 2007-10-11 2009-04-16 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for preparing porous scaffold for tissue engineering, cell culture and cell delivery
JP2012520672A (ja) * 2009-03-20 2012-09-10 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ マイクロキャリアー上の多能性(pluripotent)および多分化能(multipotent)細胞の培養
WO2014168585A1 (en) * 2013-04-10 2014-10-16 Agency For Science, Technology And Research Polycaprolactone microcarriers for stem cell culture and fabrication thereof
EP3013940B1 (en) * 2013-06-24 2017-04-12 Corning Incorporated Cell culture article and methods thereof
EP4008775A1 (en) * 2015-03-04 2022-06-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells
TWI741980B (zh) * 2015-04-07 2021-10-11 大陸商四川藍光英諾生物科技股份有限公司 一種生物磚及其用途
WO2017132358A1 (en) * 2016-01-26 2017-08-03 Kansas State University Research Foundation Methods for isolation and expansion of umbilical cord mesenchymal stem cells
SG11201909856XA (en) * 2017-05-04 2019-11-28 Mesoblast Int Sarl Mesenchymal lineage precursor or stem cells with enhanced immunosuppression
CN108715833B (zh) * 2018-06-01 2021-09-14 天晴干细胞股份有限公司 一种负载血小板裂解液的微球制备方法
KR20200015174A (ko) * 2018-08-03 2020-02-12 이동희 3차원 세포 배양 지지체 및 이를 포함하는 줄기세포 배양 조성물
AU2019368313A1 (en) * 2018-10-24 2021-05-20 Hebecell Corporation Methods and systems for manufacturing hematopoietic lineage cells
WO2020100481A1 (ja) * 2018-11-15 2020-05-22 Jsr株式会社 脳オルガノイドの製造方法
CN112522191B (zh) * 2020-12-18 2023-05-12 云南中科灵长类生物医学重点实验室 一种间充质干细胞的培养方法

Also Published As

Publication number Publication date
JP2023530098A (ja) 2023-07-13
WO2021250583A3 (en) 2022-02-24
US20230220349A1 (en) 2023-07-13
WO2021250583A2 (en) 2021-12-16
CA3181611A1 (en) 2021-12-16
EP4165166A2 (en) 2023-04-19
CN115997005A (zh) 2023-04-21
BR112022024866A2 (pt) 2023-02-14
AU2021289205A1 (en) 2023-02-02

Similar Documents

Publication Publication Date Title
AU2021254538B2 (en) Cell culture method for mesenchymal stem cells
KR20230022242A (ko) 중간엽 계통 전구세포 또는 줄기세포의 3차원 배양
GUO et al. Biological features of mesenchymal stem cells from human bone marrow
US7833785B2 (en) Method for chemical dissociation of cells from physical surfaces
EP3597729A1 (en) Cell culturing using nanofibers
US20100015710A1 (en) Methods and Compositions for Isolating, Maintaining and Serially Expanding Human Mesenchymal Stem Cells
JP2015507921A (ja) 間葉系幹細胞の培養
US20220135947A1 (en) Methods for culturing mesenchymal stem cells, products thereof, and applications thereof
EP1605040B1 (en) Monocyte-origin multipotent cell momc
KR20250134601A (ko) 조건화 배지 및 그 용도
US12359173B2 (en) Serum-free and xeno-free culture medium for expanding mesenchymal stem cells
Gottschling et al. Primitive and committed human hematopoietic progenitor cells interact with primary murine neural cells and are induced to undergo self-renewing cell divisions
HK40074091A (en) Cell culture method for mesenchymal stem cells
CA2501337C (en) Chemical dissociation of cell aggregates
HK1240971B (en) Cell culture method for mesenchymal stem cells
de Sousa Pinto Maximizing human mesenchymal stem/stromal cells therapeutic potential through 3D cell cultivation

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20230110

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20240610

Comment text: Request for Examination of Application