JP2023521198A - 酵素開発のための、超ハイスループットマイクロ流体式酵素スクリーニングプラットフォーム - Google Patents

酵素開発のための、超ハイスループットマイクロ流体式酵素スクリーニングプラットフォーム Download PDF

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JP2023521198A
JP2023521198A JP2022562285A JP2022562285A JP2023521198A JP 2023521198 A JP2023521198 A JP 2023521198A JP 2022562285 A JP2022562285 A JP 2022562285A JP 2022562285 A JP2022562285 A JP 2022562285A JP 2023521198 A JP2023521198 A JP 2023521198A
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enzyme
droplets
signal
cells
droplet
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JPWO2021209818A5 (https=
JP2023521198A5 (https=
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リ・ジー
チェン，チアウ－ホン
ジョウ，カン
リ，ジュン
リウ，ジ
カールフェカンディ，アクバル ヴァヒーディー
スン，グオユン
ウェン，ユティン
ティエン，カイヨアン
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ナショナル ユニヴァーシティー オブ シンガポール
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    • C—CHEMISTRY; METALLURGY
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    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/533—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isomerase
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/08—Methods of screening libraries by measuring catalytic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
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    • G01—MEASURING; TESTING
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    • G—PHYSICS
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G01N33/5735—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes co-enzymes or co-factors, e.g. NAD or ATP

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  • Chemical & Material Sciences (AREA)
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  • General Physics & Mathematics (AREA)
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  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Dispersion Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Plasma & Fusion (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022562285A 2020-04-13 2021-04-13 酵素開発のための、超ハイスループットマイクロ流体式酵素スクリーニングプラットフォーム Pending JP2023521198A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063008870P 2020-04-13 2020-04-13
US63/008,870 2020-04-13
US202163199500P 2021-01-04 2021-01-04
US63/199,500 2021-01-04
PCT/IB2021/000255 WO2021209818A1 (en) 2020-04-13 2021-04-13 Ultra-high-throughput microfluidic enzyme screening platform for enzyme development

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JP2023521198A true JP2023521198A (ja) 2023-05-23
JPWO2021209818A5 JPWO2021209818A5 (https=) 2024-04-22
JP2023521198A5 JP2023521198A5 (https=) 2024-04-22

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US (1) US20210318315A1 (https=)
EP (1) EP4136253A4 (https=)
JP (1) JP2023521198A (https=)
KR (1) KR20220167384A (https=)
CN (1) CN115702248A (https=)
WO (1) WO2021209818A1 (https=)

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CN114002199B (zh) * 2020-11-05 2023-01-31 上海交通大学医学院附属第九人民医院 碳氮荧光量子点在制备有氧糖酵解检测产品中的用途
EP4702154A2 (en) 2023-04-11 2026-03-04 Allozymes Pte Ltd Methods and compositions for producing a carotenoid or terpenoid
CN117761027A (zh) * 2023-12-22 2024-03-26 深圳栅极芯致生物科技有限公司 一种菌种的高通量筛选方法

Family Cites Families (8)

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Publication number Priority date Publication date Assignee Title
GB0002201D0 (en) * 2000-01-31 2000-03-22 Imperial College Process
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
JP4685611B2 (ja) * 2005-12-02 2011-05-18 株式会社エンプラス 微小流体装置
CN102517378A (zh) * 2011-11-16 2012-06-27 浙江工业大学 一种基于nad(p)h荧光的醇脱氢酶的筛选鉴定方法
WO2013078216A1 (en) * 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Glucose-6-phosphate dehydrogenase assays
CN105218583B (zh) * 2015-09-10 2017-03-29 上海交通大学 一种磷酸三酯酶的超高通量筛选方法
CN110055169A (zh) * 2019-05-07 2019-07-26 电子科技大学 一种卤醇脱卤酶文库定量筛选方法及相关微流体系统
US11536685B2 (en) * 2020-02-06 2022-12-27 Trustees Of Boston University High throughput assay for identifying microbial redox enzymes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ACS CATALYSIS, vol. 12, JPN6025024644, 19 January 2022 (2022-01-19), pages 1841 - 1846, ISSN: 0005743244 *
ACS SENSORS, vol. 2, JPN6025024643, 24 March 2017 (2017-03-24), pages 626 - 634, ISSN: 0005743243 *
JEAN-LOUIS REYMOND ET AL.: ""Screening Systems"", ADVANCES IN BIOCHEMICAL ENGINEERING/BIOTECHNOLOGY, vol. 105, JPN6025048871, 2007, pages 31 - 58, ISSN: 0005743245 *
JEAN-LOUIS REYMOND: ""Spectrophotometric Enzyme Assays for High-Throughput Screening"", FOOD TECHNOLOGY AND BIOTECHNOLOGY, vol. 42, no. 4, JPN6025048870, 2004, pages 265 - 269, ISSN: 0005743246 *
LAB ON A CHIP, vol. 20, JPN6025024642, 27 January 2020 (2020-01-27), pages 852 - 861, ISSN: 0005743242 *
LUCAS FRENZ ET AL.: ""Reliable microfluidic on-chip incubation of droplets in delay-lines"", LAB CHIP, vol. 9, no. 10, JPN6025048869, 2009, pages 1344 - 1348, ISSN: 0005743247 *

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KR20220167384A (ko) 2022-12-20
EP4136253A1 (en) 2023-02-22
EP4136253A4 (en) 2024-05-22
US20210318315A1 (en) 2021-10-14
WO2021209818A1 (en) 2021-10-21
CN115702248A (zh) 2023-02-14

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