CN115702248A - 用于酶开发的超高通量微流体酶筛选平台 - Google Patents

用于酶开发的超高通量微流体酶筛选平台 Download PDF

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Publication number
CN115702248A
CN115702248A CN202180039783.2A CN202180039783A CN115702248A CN 115702248 A CN115702248 A CN 115702248A CN 202180039783 A CN202180039783 A CN 202180039783A CN 115702248 A CN115702248 A CN 115702248A
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enzyme
signal
droplets
droplet
cells
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Chinese (zh)
Inventor
李智
陈家宏
周康
李俊
刘击
阿克巴尔·瓦希迪哈尔费坎迪
孙国运
温玉婷
田凯渊
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National University of Singapore
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National University of Singapore
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G01N33/5735—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes co-enzymes or co-factors, e.g. NAD or ATP
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/533—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isomerase
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    • C40B30/00—Methods of screening libraries
    • C40B30/08—Methods of screening libraries by measuring catalytic activity
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Dispersion Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Plasma & Fusion (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180039783.2A 2020-04-13 2021-04-13 用于酶开发的超高通量微流体酶筛选平台 Pending CN115702248A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063008870P 2020-04-13 2020-04-13
US63/008,870 2020-04-13
US202163199500P 2021-01-04 2021-01-04
US63/199,500 2021-01-04
PCT/IB2021/000255 WO2021209818A1 (en) 2020-04-13 2021-04-13 Ultra-high-throughput microfluidic enzyme screening platform for enzyme development

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Publication Number Publication Date
CN115702248A true CN115702248A (zh) 2023-02-14

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US (1) US20210318315A1 (https=)
EP (1) EP4136253A4 (https=)
JP (1) JP2023521198A (https=)
KR (1) KR20220167384A (https=)
CN (1) CN115702248A (https=)
WO (1) WO2021209818A1 (https=)

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CN114002199B (zh) * 2020-11-05 2023-01-31 上海交通大学医学院附属第九人民医院 碳氮荧光量子点在制备有氧糖酵解检测产品中的用途
EP4702154A2 (en) 2023-04-11 2026-03-04 Allozymes Pte Ltd Methods and compositions for producing a carotenoid or terpenoid
CN117761027A (zh) * 2023-12-22 2024-03-26 深圳栅极芯致生物科技有限公司 一种菌种的高通量筛选方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070125434A1 (en) * 2005-12-02 2007-06-07 Enplas Corporation Microfluidic device
CN102517378A (zh) * 2011-11-16 2012-06-27 浙江工业大学 一种基于nad(p)h荧光的醇脱氢酶的筛选鉴定方法
CN105218583A (zh) * 2015-09-10 2016-01-06 上海交通大学 一种磷酸三酯酶的超高通量筛选方法
CN110055169A (zh) * 2019-05-07 2019-07-26 电子科技大学 一种卤醇脱卤酶文库定量筛选方法及相关微流体系统

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0002201D0 (en) * 2000-01-31 2000-03-22 Imperial College Process
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
WO2013078216A1 (en) * 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Glucose-6-phosphate dehydrogenase assays
US11536685B2 (en) * 2020-02-06 2022-12-27 Trustees Of Boston University High throughput assay for identifying microbial redox enzymes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070125434A1 (en) * 2005-12-02 2007-06-07 Enplas Corporation Microfluidic device
CN102517378A (zh) * 2011-11-16 2012-06-27 浙江工业大学 一种基于nad(p)h荧光的醇脱氢酶的筛选鉴定方法
CN105218583A (zh) * 2015-09-10 2016-01-06 上海交通大学 一种磷酸三酯酶的超高通量筛选方法
CN110055169A (zh) * 2019-05-07 2019-07-26 电子科技大学 一种卤醇脱卤酶文库定量筛选方法及相关微流体系统

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FABRICE GIELEN等: "Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS)", PNAS, 12 October 2016 (2016-10-12), pages 7383 *
PETTER HAMMAR等: "Single-cell screening of photosynthetic growth and lactate production by cyanobacteria.", BIOTECHNOLOGY FOR BIOFUELS, vol. 8, no. 193, 25 November 2015 (2015-11-25), pages 1 - 8 *

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Publication number Publication date
KR20220167384A (ko) 2022-12-20
EP4136253A1 (en) 2023-02-22
EP4136253A4 (en) 2024-05-22
US20210318315A1 (en) 2021-10-14
WO2021209818A1 (en) 2021-10-21
JP2023521198A (ja) 2023-05-23

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