JP2023502018A - 無細胞ｍｅｄｉｐシークエンシングのための合成スパイクイン対照及びその使用方法 - Google Patents

無細胞ｍｅｄｉｐシークエンシングのための合成スパイクイン対照及びその使用方法 Download PDF

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JP2023502018A
JP2023502018A JP2022526702A JP2022526702A JP2023502018A JP 2023502018 A JP2023502018 A JP 2023502018A JP 2022526702 A JP2022526702 A JP 2022526702A JP 2022526702 A JP2022526702 A JP 2022526702A JP 2023502018 A JP2023502018 A JP 2023502018A
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dna
fragment
methylated
cell
control synthetic
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ウィルソン，サマンサ・エル
シェン，シュ・イー
ディニス・デ・カルヴァリョ，ダニエル
ホフマン，マイケル・エム
トリッシュ，ティモシー・ジェー
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ユニバーシティー ヘルス ネットワーク
ヴァン アンデル リサーチ インスティテュート
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JP2022526702A 2019-11-06 2020-11-06 無細胞ｍｅｄｉｐシークエンシングのための合成スパイクイン対照及びその使用方法 Pending JP2023502018A (ja)

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US201962931411P 2019-11-06 2019-11-06
US62/931,411 2019-11-06
PCT/CA2020/051507 WO2021087615A1 (en) 2019-11-06 2020-11-06 Synthetic spike-in controls for cell-free medip sequencing and methods of using same

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JP2023502018A true JP2023502018A (ja) 2023-01-20
JP2023502018A5 JP2023502018A5 (https=) 2023-11-14

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EP (2) EP4435118A3 (https=)
JP (1) JP2023502018A (https=)
KR (1) KR20220098183A (https=)
CN (1) CN115087744A (https=)
BR (1) BR112022008714A2 (https=)
CA (1) CA3157323A1 (https=)
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CN111154846A (zh) * 2020-01-13 2020-05-15 四川大学华西医院 一种甲基化核酸的检测方法
US12592321B2 (en) 2020-06-19 2026-03-31 University Health Network Cancer detection and classification using methylome analysis
CN114045342A (zh) * 2021-12-01 2022-02-15 大连晶泰生物技术有限公司 一种游离DNA(cfDNA)甲基化突变的检测方法及试剂盒
KR20250019610A (ko) * 2022-03-21 2025-02-10 빌리언투원, 인크. 치료 모니터링을 위한 메틸화된 세포 유리 dna의 분자 계수

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015009844A2 (en) * 2013-07-16 2015-01-22 Zymo Research Corp. Mirror bisulfite analysis
WO2017190215A1 (en) * 2016-05-03 2017-11-09 University Health Network Methods of capturing cell-free methylated dna and uses of same
WO2019028470A2 (en) * 2017-08-04 2019-02-07 Billiontoone, Inc. DETERMINING AND ANALYZING SEQUENCING OUTPUTS USING TARGET-ASSOCIATED MOLECULES IN QUANTIFICATION ASSOCIATED WITH BIOLOGICAL TARGETS
JP2019509068A (ja) * 2016-03-25 2019-04-04 カリウス・インコーポレイテッド 合成核酸スパイクイン
WO2019136413A1 (en) * 2018-01-08 2019-07-11 Ludwig Institute For Cancer Research Ltd Bisulfite-free, base-resolution identification of cytosine modifications

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Publication number Priority date Publication date Assignee Title
PT2850211T (pt) * 2012-05-14 2021-11-29 Irepertoire Inc Método para aumentar a precisão na deteção quantitativa de polinucleótidos
WO2018017710A1 (en) * 2016-07-19 2018-01-25 Exact Sciences Development Company, Llc Nucleic acid control molecules from non-human organisms
BR112020000681A2 (pt) * 2017-07-12 2020-07-14 University Health Network detecção e classificação de cancro utilizando análise de metilome
US20210156863A1 (en) * 2017-11-03 2021-05-27 University Health Network Cancer detection, classification, prognostication, therapy prediction and therapy monitoring using methylome analysis

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015009844A2 (en) * 2013-07-16 2015-01-22 Zymo Research Corp. Mirror bisulfite analysis
JP2019509068A (ja) * 2016-03-25 2019-04-04 カリウス・インコーポレイテッド 合成核酸スパイクイン
WO2017190215A1 (en) * 2016-05-03 2017-11-09 University Health Network Methods of capturing cell-free methylated dna and uses of same
JP2019521315A (ja) * 2016-05-03 2019-07-25 ユニバーシティ・ヘルス・ネットワーク 無細胞メチル化ｄｎａを捕捉する方法及びその使用
WO2019028470A2 (en) * 2017-08-04 2019-02-07 Billiontoone, Inc. DETERMINING AND ANALYZING SEQUENCING OUTPUTS USING TARGET-ASSOCIATED MOLECULES IN QUANTIFICATION ASSOCIATED WITH BIOLOGICAL TARGETS
WO2019136413A1 (en) * 2018-01-08 2019-07-11 Ludwig Institute For Cancer Research Ltd Bisulfite-free, base-resolution identification of cytosine modifications

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NAT. PROTOC., vol. 14, JPN6025000088, October 2019 (2019-10-01), pages 2749 - 2780, ISSN: 0005502547 *

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GB2609715A (en) 2023-02-15
GB202207732D0 (en) 2022-07-13
US20230024827A1 (en) 2023-01-26
EP4055183A1 (en) 2022-09-14
BR112022008714A2 (pt) 2022-07-19
WO2021087615A1 (en) 2021-05-14
CN115087744A (zh) 2022-09-20
EP4055183A4 (en) 2023-12-06
EP4435118A3 (en) 2025-01-15
EP4435118A2 (en) 2024-09-25
CA3157323A1 (en) 2021-05-14
KR20220098183A (ko) 2022-07-11
GB2609715B (en) 2025-07-30

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