BR112022008714A2 - Controles de picos sintéticos para sequenciamento medip livre de células e métodos de uso dos mesmos - Google Patents

Controles de picos sintéticos para sequenciamento medip livre de células e métodos de uso dos mesmos

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Publication number
BR112022008714A2
BR112022008714A2 BR112022008714A BR112022008714A BR112022008714A2 BR 112022008714 A2 BR112022008714 A2 BR 112022008714A2 BR 112022008714 A BR112022008714 A BR 112022008714A BR 112022008714 A BR112022008714 A BR 112022008714A BR 112022008714 A2 BR112022008714 A2 BR 112022008714A2
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Brazil
Prior art keywords
cell
free
sample
methylated
methods
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BR112022008714A
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English (en)
Inventor
L Wilson Samantha
Yi Shen Shu
Diniz De Carvalho Daniel
M Hoffman Michael
J Triche Timothy
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Univ Health Network
Van Andel Res Institute
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Application filed by Univ Health Network, Van Andel Res Institute filed Critical Univ Health Network
Publication of BR112022008714A2 publication Critical patent/BR112022008714A2/pt

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
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    • C12Q2545/00—Reactions characterised by their quantitative nature
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
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Abstract

CONTROLES DE PICOS SINTÉTICOS PARA SEQUENCIAMENTO MEDIP LIVRE DE CÉLULAS E MÉTODOS DE USO DOS MESMOS. É aqui descrito um método de captura e análise de DNA metilado livre de células em uma amostra. O método envolve submeter a amostra à preparação da biblioteca para permitir o sequenciamento subsequente do DNA metilado livre de células. Uma quantidade predeterminada de fragmentos de DNA sintético de controle é adicionada à amostra. Os fragmentos de DNA sintético de controle têm, cada um, uma sequência de ácido nucleico conhecida que não se alinha a uma sequência de genoma alvo e pelo menos alguns dos fragmentos de DNA sintético de controle são metilados. A amostra é desnaturada e o DNA metilado livre de células e os fragmentos de DNA sintético de controle são capturados usando um aglutinante seletivo para polinucleotídeos metila-dos. O DNA capturado é amplificado e sequenciado.
BR112022008714A 2019-11-06 2020-11-06 Controles de picos sintéticos para sequenciamento medip livre de células e métodos de uso dos mesmos BR112022008714A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962931411P 2019-11-06 2019-11-06
PCT/CA2020/051507 WO2021087615A1 (en) 2019-11-06 2020-11-06 Synthetic spike-in controls for cell-free medip sequencing and methods of using same

Publications (1)

Publication Number Publication Date
BR112022008714A2 true BR112022008714A2 (pt) 2022-07-19

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ID=75849048

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BR112022008714A BR112022008714A2 (pt) 2019-11-06 2020-11-06 Controles de picos sintéticos para sequenciamento medip livre de células e métodos de uso dos mesmos

Country Status (9)

Country Link
US (1) US20230024827A1 (pt)
EP (1) EP4055183A4 (pt)
JP (1) JP2023502018A (pt)
KR (1) KR20220098183A (pt)
CN (1) CN115087744A (pt)
BR (1) BR112022008714A2 (pt)
CA (1) CA3157323A1 (pt)
GB (1) GB2609715A (pt)
WO (1) WO2021087615A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111154846A (zh) * 2020-01-13 2020-05-15 四川大学华西医院 一种甲基化核酸的检测方法
CN114045342A (zh) * 2021-12-01 2022-02-15 大连晶泰生物技术有限公司 一种游离DNA(cfDNA)甲基化突变的检测方法及试剂盒

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11078475B2 (en) * 2016-05-03 2021-08-03 Sinai Health System Methods of capturing cell-free methylated DNA and uses of same
CA3069754A1 (en) * 2017-07-12 2019-01-17 University Health Network Cancer detection and classification using methylome analysis
EP3704267A4 (en) * 2017-11-03 2021-08-04 University Health Network DETECTION, CLASSIFICATION, PROGNOSIS, THERAPY PREDICTION AND MONITORING OF CANCER THERAPY USING METHYLOMA TEST

Also Published As

Publication number Publication date
KR20220098183A (ko) 2022-07-11
JP2023502018A (ja) 2023-01-20
EP4055183A1 (en) 2022-09-14
CA3157323A1 (en) 2021-05-14
CN115087744A (zh) 2022-09-20
WO2021087615A1 (en) 2021-05-14
US20230024827A1 (en) 2023-01-26
GB2609715A (en) 2023-02-15
EP4055183A4 (en) 2023-12-06
GB202207732D0 (en) 2022-07-13

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