CA3157323A1 - Synthetic spike-in controls for cell-free medip sequencing and methods of using same - Google Patents

Synthetic spike-in controls for cell-free medip sequencing and methods of using same Download PDF

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Publication number
CA3157323A1
CA3157323A1 CA3157323A CA3157323A CA3157323A1 CA 3157323 A1 CA3157323 A1 CA 3157323A1 CA 3157323 A CA3157323 A CA 3157323A CA 3157323 A CA3157323 A CA 3157323A CA 3157323 A1 CA3157323 A1 CA 3157323A1
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Prior art keywords
dna
methylated
fragments
fragment
cell
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English (en)
French (fr)
Inventor
Samantha L. WILSON
Shu Yi Shen
Daniel Diniz De Carvalho
Michael M. HOFFMAN
Timothy J. Triche
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University Health Network
Van Andel Research Institute
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University Health Network
Van Andel Research Institute
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CA3157323A 2019-11-06 2020-11-06 Synthetic spike-in controls for cell-free medip sequencing and methods of using same Pending CA3157323A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962931411P 2019-11-06 2019-11-06
US62/931,411 2019-11-06
PCT/CA2020/051507 WO2021087615A1 (en) 2019-11-06 2020-11-06 Synthetic spike-in controls for cell-free medip sequencing and methods of using same

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CA3157323A1 true CA3157323A1 (en) 2021-05-14

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US (1) US20230024827A1 (https=)
EP (2) EP4435118A3 (https=)
JP (1) JP2023502018A (https=)
KR (1) KR20220098183A (https=)
CN (1) CN115087744A (https=)
BR (1) BR112022008714A2 (https=)
CA (1) CA3157323A1 (https=)
GB (1) GB2609715B (https=)
WO (1) WO2021087615A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114045342A (zh) * 2021-12-01 2022-02-15 大连晶泰生物技术有限公司 一种游离DNA(cfDNA)甲基化突变的检测方法及试剂盒

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111154846A (zh) * 2020-01-13 2020-05-15 四川大学华西医院 一种甲基化核酸的检测方法
US12592321B2 (en) 2020-06-19 2026-03-31 University Health Network Cancer detection and classification using methylome analysis
KR20250019610A (ko) * 2022-03-21 2025-02-10 빌리언투원, 인크. 치료 모니터링을 위한 메틸화된 세포 유리 dna의 분자 계수

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PT2850211T (pt) * 2012-05-14 2021-11-29 Irepertoire Inc Método para aumentar a precisão na deteção quantitativa de polinucleótidos
EP3022321B1 (en) * 2013-07-16 2021-01-13 Zymo Research Corporation Mirror bisulfite analysis
EP3978627A1 (en) * 2016-03-25 2022-04-06 Karius, Inc. Methods using synthetic nucleic acid spike-ins
KR102522067B1 (ko) * 2016-05-03 2023-04-13 유니버시티 헬스 네트워크 무-세포 메틸화된 dna의 포획 방법 및 이의 이용
WO2018017710A1 (en) * 2016-07-19 2018-01-25 Exact Sciences Development Company, Llc Nucleic acid control molecules from non-human organisms
BR112020000681A2 (pt) * 2017-07-12 2020-07-14 University Health Network detecção e classificação de cancro utilizando análise de metilome
CN111094583A (zh) * 2017-08-04 2020-05-01 十亿至一公司 与生物靶相关的定量中利用靶相关分子的测序输出确定和分析
US20210156863A1 (en) * 2017-11-03 2021-05-27 University Health Network Cancer detection, classification, prognostication, therapy prediction and therapy monitoring using methylome analysis
NZ765943A (en) * 2018-01-08 2022-10-28 Ludwig Inst For Cancer Res Ltd Bisulfite-free, base-resolution identification of cytosine modifications

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114045342A (zh) * 2021-12-01 2022-02-15 大连晶泰生物技术有限公司 一种游离DNA(cfDNA)甲基化突变的检测方法及试剂盒
WO2023098137A1 (zh) * 2021-12-01 2023-06-08 大连晶泰生物技术有限公司 一种游离dna甲基化突变的检测方法及试剂盒
GB2620492A (en) * 2021-12-01 2024-01-10 Dalian Gentalker Bio Tech Co Ltd Method and kit for detecting methylation mutation of free DNA

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GB2609715A (en) 2023-02-15
GB202207732D0 (en) 2022-07-13
US20230024827A1 (en) 2023-01-26
EP4055183A1 (en) 2022-09-14
BR112022008714A2 (pt) 2022-07-19
WO2021087615A1 (en) 2021-05-14
JP2023502018A (ja) 2023-01-20
CN115087744A (zh) 2022-09-20
EP4055183A4 (en) 2023-12-06
EP4435118A3 (en) 2025-01-15
EP4435118A2 (en) 2024-09-25
KR20220098183A (ko) 2022-07-11
GB2609715B (en) 2025-07-30

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