GB2609715B - Synthetic spike-in controls for cell-free MeDIP sequencing and methods of using same - Google Patents

Synthetic spike-in controls for cell-free MeDIP sequencing and methods of using same

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Publication number
GB2609715B
GB2609715B GB2207732.5A GB202207732A GB2609715B GB 2609715 B GB2609715 B GB 2609715B GB 202207732 A GB202207732 A GB 202207732A GB 2609715 B GB2609715 B GB 2609715B
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cell
controls
free
methods
same
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GB2609715A (en
GB202207732D0 (en
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L Wilson Samantha
Yi Shen Shu
Diniz De Carvalho Daniel
M Hoffman Michael
J Triche Timothy
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University Health Network
Van Andel Research Institute
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University Health Network
Van Andel Research Institute
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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GB2207732.5A 2019-11-06 2020-11-06 Synthetic spike-in controls for cell-free MeDIP sequencing and methods of using same Active GB2609715B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962931411P 2019-11-06 2019-11-06
PCT/CA2020/051507 WO2021087615A1 (en) 2019-11-06 2020-11-06 Synthetic spike-in controls for cell-free medip sequencing and methods of using same

Publications (3)

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GB202207732D0 GB202207732D0 (en) 2022-07-13
GB2609715A GB2609715A (en) 2023-02-15
GB2609715B true GB2609715B (en) 2025-07-30

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GB2207732.5A Active GB2609715B (en) 2019-11-06 2020-11-06 Synthetic spike-in controls for cell-free MeDIP sequencing and methods of using same

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US (1) US20230024827A1 (https=)
EP (2) EP4435118A3 (https=)
JP (1) JP2023502018A (https=)
KR (1) KR20220098183A (https=)
CN (1) CN115087744A (https=)
BR (1) BR112022008714A2 (https=)
CA (1) CA3157323A1 (https=)
GB (1) GB2609715B (https=)
WO (1) WO2021087615A1 (https=)

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CN111154846A (zh) * 2020-01-13 2020-05-15 四川大学华西医院 一种甲基化核酸的检测方法
US12592321B2 (en) 2020-06-19 2026-03-31 University Health Network Cancer detection and classification using methylome analysis
CN114045342A (zh) * 2021-12-01 2022-02-15 大连晶泰生物技术有限公司 一种游离DNA(cfDNA)甲基化突变的检测方法及试剂盒
KR20250019610A (ko) * 2022-03-21 2025-02-10 빌리언투원, 인크. 치료 모니터링을 위한 메틸화된 세포 유리 dna의 분자 계수

Citations (3)

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WO2017190215A1 (en) * 2016-05-03 2017-11-09 University Health Network Methods of capturing cell-free methylated dna and uses of same
WO2019010564A1 (en) * 2017-07-12 2019-01-17 University Health Network DETECTION AND CLASSIFICATION OF CANCER USING METHYLOME ANALYSIS
WO2019084659A1 (en) * 2017-11-03 2019-05-09 University Health Network Cancer detection, classification, prognostication, therapy prediction and therapy monitoring using methylome analysis

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PT2850211T (pt) * 2012-05-14 2021-11-29 Irepertoire Inc Método para aumentar a precisão na deteção quantitativa de polinucleótidos
EP3022321B1 (en) * 2013-07-16 2021-01-13 Zymo Research Corporation Mirror bisulfite analysis
EP3978627A1 (en) * 2016-03-25 2022-04-06 Karius, Inc. Methods using synthetic nucleic acid spike-ins
WO2018017710A1 (en) * 2016-07-19 2018-01-25 Exact Sciences Development Company, Llc Nucleic acid control molecules from non-human organisms
CN111094583A (zh) * 2017-08-04 2020-05-01 十亿至一公司 与生物靶相关的定量中利用靶相关分子的测序输出确定和分析
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WO2017190215A1 (en) * 2016-05-03 2017-11-09 University Health Network Methods of capturing cell-free methylated dna and uses of same
WO2019010564A1 (en) * 2017-07-12 2019-01-17 University Health Network DETECTION AND CLASSIFICATION OF CANCER USING METHYLOME ANALYSIS
WO2019084659A1 (en) * 2017-11-03 2019-05-09 University Health Network Cancer detection, classification, prognostication, therapy prediction and therapy monitoring using methylome analysis
CA3080215A1 (en) * 2017-11-03 2019-05-09 University Health Network Cancer detection, classification, prognostication, therapy prediction and therapy monitoring using methylome analysis

Non-Patent Citations (5)

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Title
Chen K. et al., The Overlooked Fact: Fundamental Need for Spike-In Control for Virtually All Genome-Wide Analyses. Molecular and Cellular Biology, March 2016 (03-2016), Volume 36, Number 5, Pages 662-667. ISSN: 0270-7306 Whole Document. *
Jiang L. et al., Synthetic spike-in standards for RNA-seq experiments. Genome Research, 2011, Volume 21, Pages 1543-1551. ISSN: 1088-9051 Whole Document. *
Shen S.Y. et al., Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA. Nature Protocols, October 2019 (10-2019), Volume 14, Pages 2749-2780. ISSN: 1754-2189 Whole Document *
Taiwo, O et al., Methylome analysis using MeDIP-seq with low DNA concentrations. Nature Protocols, 8 March 2012 (08-03-2012), Volume 7, Number 4, Pages 617-636. ISSN: 1754-2189 *
Zhao M-T. et al., Methylated DNA Immunoprecipitation and High-Throughput Sequencing (MeDIP-seq) Using Low Amounts of Genomic DNA. Cellular Reprogramming, 2014, Volume 16, Number 3, Pages 175 - 184. ISSN: 2152-4971 *

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Publication number Publication date
GB2609715A (en) 2023-02-15
GB202207732D0 (en) 2022-07-13
US20230024827A1 (en) 2023-01-26
EP4055183A1 (en) 2022-09-14
BR112022008714A2 (pt) 2022-07-19
WO2021087615A1 (en) 2021-05-14
JP2023502018A (ja) 2023-01-20
CN115087744A (zh) 2022-09-20
EP4055183A4 (en) 2023-12-06
EP4435118A3 (en) 2025-01-15
EP4435118A2 (en) 2024-09-25
CA3157323A1 (en) 2021-05-14
KR20220098183A (ko) 2022-07-11

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