JP2023164630A - Oral composition - Google Patents

Abstract

To provide an oral composition having high sound sleep effect.SOLUTION: An oral composition contains theanine, and at least one other component selected from pu-erh and jasmine.SELECTED DRAWING: Figure 1

Description

Application for application of Article 30, Paragraph 2 of the Patent Act has been filed Publication start date: March 7, 2016 Publication address: http://www. caa. go. jp/foods/docs/ichiran. xls https://www. fld. caa. go. jp/caaks/cssc02/pdf/A244-ippan. pdf https://www. fld. caa. go. jp/caaks/cssc02/pdf/A244-kihon. pdf https://www. fld. caa. go. jp/caaks/cssc02/pdf/A244-kinou. pdf https://www. fld. caa. go. jp/caaks/cssc02/pdf/A244-anzen. pdf

TECHNICAL FIELD The present invention relates to compositions for use in restful sleep, and in particular to oral compositions containing theanine and certain other ingredients.

In modern society, autonomic nerves are disrupted due to various factors such as changes in lifestyle and increased stress, and people often suffer from insomnia. Insomnia has various effects such as decreased concentration and work efficiency, fatigue such as tiredness and sluggishness, physical discomfort, and depression, so it is important to have a good quality and restful sleep from a social and health perspective. It has been demanded.

Examples of such compositions for restful sleep include lavender steam distilled water (see Patent Document 1), compositions containing hastatoside (see Patent Document 2), and compositions containing verbenaline (see Patent Document 3). ) has been proposed.

Japanese Patent Application Publication No. 2006-104073 Japanese Patent Application Publication No. 2005-154311 Japanese Patent Application Publication No. 2005-154310

An object of the present invention is to provide a composition that has a high sleep effect.

As a result of intensive investigation and research into further improving the function of theanine, which has a sleep-inducing effect, the present inventors discovered that a higher sleep-rest effect can be obtained by combining theanine with a specific ingredient, thereby completing the present invention. reached.

That is, the present invention relates to an oral composition characterized by containing theanine and at least one other component selected from the group consisting of (a) to (b) below.
(a) At least one plant material selected from barley, sweet potato, oat, rice, rooibos, puerh, jasmine, and Terminaria (b) At least one functional material selected from lactic acid bacteria and fructooligosaccharide

The present invention also relates to a functional food composition for good sleep, which contains theanine as an active ingredient and contains at least one other ingredient selected from the group consisting of (a) to (b) above.

The present invention relates to a functional food composition for promoting sound sleep, which contains theanine as an active ingredient and contains at least one other ingredient selected from the group consisting of (a) to (b) above.

The present invention provides a sleep aid that suppresses the influx of Ca ions into nerve cells, which contains theanine and at least one other component selected from the group consisting of (a) to (b) above. The present invention relates to functional food compositions.

The present invention relates to a functional food composition for a good night's sleep, which contains at least one other ingredient selected from the group consisting of (a) to (b) above, which promotes theanine to suppress the influx of Ca ions into nerve cells. .

The present invention provides a functional food for sleep that binds to glutamate receptors of nerve cells, which is characterized by containing theanine and at least one other ingredient selected from the group consisting of (a) to (b) above. Regarding the composition.

Furthermore, the present invention provides a functional food composition for good sleep containing at least one other ingredient selected from the group consisting of (a) to (b) above that promotes the binding of theanine to glutamate receptors of nerve cells. Regarding.

The present invention relates to the production of a functional food composition for good sleep, characterized in that an oral composition containing theanine contains at least one other ingredient selected from the group consisting of (a) to (b) above. Regarding the method.

The oral composition of the present invention can provide a high sleep effect by suppressing nervous excitement.

FIG. 2 is a diagram showing the results of intracellular Ca fluorescence intensity change ratio when the oral composition of the present invention (theanine + other components) is applied to primary rat cerebral cortex-derived neurons.

The composition of the present invention is characterized by containing theanine and at least one component (hereinafter sometimes referred to as other component) selected from the group consisting of (a) to (b) below. Components (a) to (b) used together with theanine may be used alone (for example, only component (a)) or in combination of two or more. When using a combination of two or more other components, it is preferable to combine other components that have a high synergistic effect with theanine.

The oral composition of the present invention uses a combination of theanine and other ingredients to increase inhibitory neurotransmitters and suppress the action of excitatory neurotransmitters, effectively suppressing nerve excitation and preventing excessive excitation. It can help you sleep well and relax.

[Theanine]
The theanine in the oral composition of the present invention is preferably L-theanine, and any method for producing it may be used, including those obtained by extraction from plants, those obtained by chemical synthesis, and those obtained by fermentation. Theanine is commercially available as a reagent, food additive, etc., and commercially available products can be used.

[Other ingredients]
(a) Plant material In the oral composition of the present invention, it is preferable to use at least one plant material selected from barley, sweet potato, oat, rice, rooibos, puerh, jasmine, and terminaria together with theanine.

These plant materials may be any part of the plant such as leaves, stems, roots, flowers, fruits, trunks, branches, etc. In addition to the plant itself, it may also include dried, crushed, squeezed, extracted products, etc. A processed plant product can be used. Examples of the pulverized product include powder, granules, and the like. The squeezed juice or extract may be in liquid form, but it can also be used in the form of a paste or dry powder. The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent include water (warm water, hot water), ethanol, and aqueous ethanol. Commercially available plant materials may be used.

(barley)
Barley is a plant of the genus Barley of the Poaceae family, which is native to Central Asia, and two-rowed barley, six-rowed barley, etc. can be used. The parts used as the plant material of the present invention are preferably stems and leaves, and more preferably young leaves. Particularly preferred are young leaf powder obtained by drying and pulverizing young leaves, and extracts from young leaves.

(sweet potato)
Sweet potato refers to a plant belonging to the Convolvulaceae family, and is generally called sweet potato. The varieties of sweet potato are not particularly limited, and examples include Suio, Joy White, Koganesengan, Shiro Yutaka, Sweet Mustache, and Ayamurasaki. The parts used as the plant material of the present invention are preferably stems and leaves, and more preferably young leaves. Particularly preferred are young leaf powder obtained by drying and pulverizing young leaves, and extracts from young leaves.

(oats)
Oats are a plant belonging to the Poaceae family, Avena genus, and are native to Patagonia, Chile, and their scientific name is Avena sativa. The parts used as the plant material of the present invention are preferably ears, stems, and leaves, and more preferably dry powder obtained by drying and pulverizing the ears, stems, and leaves, and extracts from the ears, stems, and leaves.

(rice)
Rice is a plant belonging to the Poaceae family, Poaceae. As the plant material of the present invention, rice (brown rice) is preferable, and rice milk produced from rice is particularly preferable. There are no particular restrictions on the type of rice, and examples include japonica and indica.

(Terminaria)
Examples of Terminalia include Terminalia bellirica (belerica), Terminalia catappa, Terminalia tomentosa, Terminalia citrina, Terminalia phellocarpa, Terminalia copelandii, Terminalia brassi, Terminalia ivorensis, Terminalia superba, Terminalia arjuna, Terminalia chebula, etc. Among them, Terminalia bellirica (belerica) is preferred. The part used as the plant material of the present invention is preferably a fruit. Particularly preferred are dry powders obtained by drying and pulverizing fruits and extracts from fruits.

(Rooibos)
Rooibos is a plant belonging to the genus Asparatus of the Fabaceae family, and preferred plant materials in the present invention are leaves and branches that are usually used for tea. Particularly preferred are dried powders obtained by drying and pulverizing leaves and twigs, and extracts from leaves and twigs.

(Puerh)
Pu'er is a type of Chinese tea (black tea) originating from Yunnan Province, People's Republic of China, and includes raw tea and mature tea. As the plant material in the present invention, leaves that are usually used as tea are preferred. Particularly preferred are dried powders obtained by drying and pulverizing leaves, and extracts from leaves.

(jasmine)
Jasmine is a plant belonging to the Oleaceae family, and as the plant material in the present invention, flowers that are usually used for tea are preferred. Particularly preferred are dried powders obtained by drying and pulverizing flowers and extracts from flowers.
(b) Functional material In the oral composition of the present invention, it is preferable to use at least one functional material selected from lactic acid bacteria and fructooligosaccharides together with theanine.

(lactic acid bacteria)
Lactic acid bacteria include Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium mongoliense, Lactbacillus brevis, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus buchneri, Lactobacterium obacillus bulgaricus, Lactobacillus delburvecki, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus halivaticus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacilus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus hilgardii, Lactobacillus reuteri, Lactobacillus fermentum, Enteroc occus faecalis (sometimes called Streptococcus faecalis) ), ENTEROCOCCUS FAESIUM (sometimes referred to as Streptococcus Faesium), Streptococcus Thermophilus, Lactococcus Lactis (sometimes referred to as Streptococcus Lactis), Leu Conostoc Mesenteroids, Leuconostoc Oenos, Pediococcus Acidilactici, Pediococcus Pentosaceus, Staphylococcus Carnosus, Staphyloccus XYLO SUS , Tetragenococcus halophilus, Bacillus coagulans, Bacillus mesentericus, etc., Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus plantarum, L actobacillus fermentum, Lactobacillus paracasei, Enterococcus faecalis ( Preferred are Streptococcus faecalis, Enterococcus faesium, Streptococcus thermophilus, Lactococcus lactis, Bacillus coagulans, and Bacillus mesentericus. These lactic acid bacteria can be used alone or in combination of two or more. Depending on the dosage form and quality of the composition of the present invention, for example, those having properties such as heat resistance, acid resistance, sugar resistance, salt resistance, and sporulation can be appropriately selected. The method for obtaining lactic acid bacteria is not particularly limited, and for example, lactic acid bacteria isolated from foods such as yogurt and vegetables or commercially available products can be used.

These other ingredients function as promoters of theanine's sleep effect.

The oral composition of the present invention includes, for example, pharmaceuticals (including quasi-drugs), foods for specified health uses, foods with nutritional function claims, and functional foods for which efficacy claims have been approved by designated organizations, such as foods with functional claims. Examples include so-called health foods such as, general foods, food additives, feed, etc. The oral composition of the present invention can be taken by anyone who is concerned about the above effects, regardless of gender or age. It is also desirable to take it 30 minutes to 2 hours before bedtime.

The oral composition of the present invention can be used as a sound sleep composition used for sound sleep, and such a sound sleep composition contains theanine and other predetermined ingredients, and is different from other products as a product in that it is used for sound sleep. There is no particular restriction as long as it can be distinguished from the above. For example, the scope of the present invention includes products in which the product body, packaging, instruction manual, or advertising material of the present invention indicates that the product has a good sleep effect. Note that the composition for restful sleep of the present invention is not limited to one in which the combined ingredients (theanine and other predetermined ingredients) of the present invention are indicated as active ingredients for restful sleep on the product packaging or the like. For example, the active ingredient may not be specified, theanine and other predetermined ingredients may be indicated as the active ingredient, or theanine alone may be indicated as the active ingredient.

Specifically, the composition for restful sleep of the present invention includes pharmaceuticals (including quasi-drugs) and so-called health foods. ``Reduces drowsiness and drowsiness'', ``Reduces the feeling of fatigue (feeling tired and sluggish) when waking up the next morning'', ``Produces healthy sleep'', ``Wakes up refreshed'', ``Reduces drowsiness and fatigue when waking up'' Examples include those displaying the following phrases: ``Helpful for restoring the senses'', ``Supporting quality sleep at night'', ``Supporting healthy sleep'', ``Supporting healthy sleep at night'', etc.

The oral composition of the present invention may be in the form of a tablet, capsule, powder, granule, liquid, granule, rod, plate, block, solid, round, paste, cream, or caplet. Examples include shape, gel shape, chewable shape, and stick shape. Among these, tablet, capsule, powder, granule, and liquid forms are particularly preferred. Specifically, supplements, packaged drinks filled in plastic bottles, cans, bottles, etc., instant drinks that are dissolved in water (hot water), milk, fruit juice, green juice, etc., and food additives. can be exemplified. These are preferable because they are easy to drink during meals and can enhance palatability.

The content of theanine and other components (components of the present invention) in the oral composition of the present invention may be appropriately contained within the range where their effects can be achieved.

Generally, when the oral composition of the present invention is a drug or a supplement, it is preferable that the component of the present invention is contained in an amount of 0.1 to 100% by weight, and 1 to 85% by weight, calculated as a dry weight. %, and even more preferably 5 to 70% by mass.

When the oral composition of the present invention is a packaged beverage, the component of the present invention is preferably contained in an amount of 0.0001 to 30% by weight, and preferably 0.001 to 25% by weight, based on dry weight. It is more preferable that the content is 0.01 to 20% by mass.

In addition, when the oral composition of the present invention is an instant drink, the component of the present invention is preferably contained in an amount of 0.001 to 80% by mass, and preferably 0.005 to 70% by mass in terms of dry mass. It is more preferable that it is contained, and even more preferably that it is contained in an amount of 0.1 to 60% by mass.

In order to more effectively exhibit the effects of the present invention, it is preferable that the ingredients of the present invention are contained in an amount of 80% or more, and preferably 90% or more, of the entire oral composition of the present invention in terms of dry mass. It is more preferable that the content is 95% or more, and it is particularly preferable that the content is 100%.

There is no particular limit to the amount of the oral composition of the present invention to be ingested, but from the perspective of more significantly exerting the effects of the present invention, the daily intake of the ingredients of the present invention by adults should be 50 mg/day or more. It is preferable to take in an amount of 100 mg/day or more, more preferably 150 mg/day or more. The upper limit is not particularly limited, but is, for example, 10,000 mg/day, preferably 5,000 mg/day.

The oral composition of the present invention can be stored in one container or divided into a plurality of containers, for example, 2 to 3, so that the daily intake amount is the above-mentioned intake amount.

The blending mass ratio of theanine and other components is preferably in the range of 1:0.001 to 1:50, more preferably in the range of 1:0.01 to 1:40, in terms of dry mass. , more preferably in the range of 1:0.1 to 1:30, particularly preferably in the range of 1:1 to 1:20. When the blending ratio of theanine and other components is within the above range, the effects of the present invention can be more effectively exhibited.

The oral composition of the present invention can be produced by a known method by adding, if necessary, components other than the components of the present invention that are acceptable for oral use.

The pH when the oral composition of the present invention is dissolved in water is not particularly limited, but is preferably in the range of 2.0 to 7.0, more preferably 3.0 to 6.5, and more preferably 4.5 to 6. 5 is most preferred.

Hereinafter, the present invention will be explained based on examples.
[Example 1]
1. cell culture
(1) Primary rat cerebral cortex-derived neurons (manufactured by Thermo) were coated with Poly-L-Lysine at a density of 2 μg/cm ² in each well of a 96-well black plate at a cell density of 1.0×10 ⁴ cells/well. It was sown in
(2) One week of pre-culture at 37°C in a CO ₂ incubator. Medium exchange was performed every 1 to 2 days.
(3) After removing the medium from each well, 100 μL of a test substance-containing medium prepared at a predetermined concentration using a normal medium was added and cultured in a CO ₂ incubator for 24 hours.
The normal medium used was 48.875 ml of Neurobasal medium (manufactured by Thermo) to which 125 μl of 200 mM Glutamax ^TM (manufactured by Thermo) and 1 ml of B27 Serum Free Supplement (50x) (manufactured by Thermo) were added.

As for theanine, commercially available L-theanine was used. As other components used together with L-theanine, the substances shown in Table 1 were used.

2. Evaluation of intracellular Ca

Generally, when the NMDA receptor (glutamate receptor, an excitatory amino acid) on the surface of a nerve cell is activated by an excitatory amino acid (glutamate), a large amount of Ca ions flows into the nerve cell, and then It is said that various Ca ion-dependent enzymes are activated and nerve overexcitation occurs. In this test, the effect of the test substance on suppressing the influx of Ca ions into nerve cells was investigated.

Specifically, the following tests were conducted.
Intracellular Ca was measured using Calcium-Kit Fura2 (manufactured by Dojindo Laboratories).
(1) After culturing for 24 hours, the medium was removed from each well, washed once with PBS(-), 100 μL of Loading Buffer was added, and cultured for 1 hour.
(2) Loading Buffer was removed, the plate was washed once with PBS (-), 90 μL of Recording Buffer was added, and the plate was acclimatized in an incubator for 10 minutes.

(3) Add 10 μL of 250 μM Glutamate to a final concentration of 25 μM, and 1 minute after addition (2) Sample fluorescence intensity Ftest (340) (excitation wavelength 340 nm, fluorescence wavelength 510 nm), Ftest (380) (excitation wavelength The wavelength was 380 nm, and the fluorescence wavelength was 510 nm). Furthermore, the fluorescence intensities Fpre (340) and Fpre (380) immediately before the addition of Glutamate were measured in the same manner. Furthermore, after the test was completed, MnCl ₂ was added to give a final concentration of 0.33 M, and the fluorescence intensities Fblank (340) and Fblank (380) were similarly measured to calculate autofluorescence.
(4) Based on the measured values, the change value of the fluorescence intensity ratio was calculated using the following formula.

Change value of Δ fluorescence intensity ratio = Rtest - Rpre
・Rtest = (Ftest(340) - Fblank(340)) / (Ftest(380) - Fblank(380))
・Rpre = (Fpre(340) - Fblank(340)) / (Fpre(380) - Fblank(380))

The results are shown in Table 1 and FIG. In FIG. 1, the numerical values written after the component names indicate the final concentrations (μg/mL) of other components. For example, "Barley_200" in FIG. 1 indicates 200 (μg/mL). The vertical axis indicates "intracellular Ca fluorescence intensity change ratio."

For barley, dry and ground powder of young leaves was used.
For sweet potato, dried crushed powder of young leaves was used.
For oats, oat green manufactured by Toyo Shinyaku Co., Ltd., which is an extract from the entire above-ground part of the plant including ears and stems and leaves, was used.
Regarding rice (rice milk), commercially available rice milk was used.
For the rooibos, commercially available ground rooibos tea powder was used.
For Pu-erh, commercially available ground Pu-erh tea powder was used.
As for jasmine, commercially available ground jasmine tea powder was used.
For Terminaria, a hot water extract (dry powder) of Terminaria berrilica fruit was used.
As for lactic acid bacteria, Bacillus coagulans was used.
As for the fructooligosaccharide, a commercially available fructooligosaccharide was used.
For mugwort, dried and ground powder of leaves was used.
For lavender, commercially available crushed lavender tea powder was used.

As shown in Table 1 and FIG. 1, it was confirmed that the combination of L-theanine and certain other components reduced the intracellular Ca fluorescence intensity ratio. That is, it is considered that the combined components of the present invention antagonized the stimulation of glutamate to NMDA receptors and suppressed the influx of Ca ions into nerve cells. Therefore, the composition of the present invention can suppress nervous overexcitation and provide a high level of sleep effect.

[Example 2] (Manufacture of tablets)
Five tablets consisting of the following ingredients were manufactured.

Oat dry extract powder 400mg
Fructooligosaccharide 250mg
L-theanine 200mg
Hydroxypropylcellulose 350mg
Calcium stearate 250mg
Silicon dioxide 50mg

The above tablets are taken once a day or in two or three divided doses with water.

[Example 3] (Manufacture of capsules)
The following mixture was encapsulated in soft capsules to produce capsules.

L-theanine 200mg
Terminalia Berylica powder 100mg
Lactic acid bacteria 400mg
Maltose 50mg
Silicon dioxide 10mg

The above capsules are taken in 4 tablets once a day or in 2 to 4 divided doses with water.

[Example 4] (Manufacture of granules)
Granules (3000 mg) were prepared by mixing the following ingredients in a conventional manner.

Barley grass dry extract powder 1200mg
Theanine 200mg
Sucralose 150mg
Thiamine hydrochloride 10mg
Riboflavin 10mg
Vitamin B6 5mg
Cyanocobalamin 6 5mg
Fragrance 5mg
Reduced Palatinose 200mg
Calcium stearate 100mg
Hydroxypropyl cellulose balance

[Example 5] (Production of instant powder)
An instant powder (2 g) was prepared by mixing the following ingredients in a conventional manner.

Rooibos tea processed food 800mg
Theanine 200mg
Aspartame 150mg
Sucralose 50mg
Malic acid 10mg
Pigment preparation 10mg
Fragrance 5mg
Polydextrose remainder

[Example 6] (Production of liquid agent)
A liquid preparation (200 mL) consisting of the following components was produced.

Theanine 200mg
Pu-erh tea processed food 250mg
Jasmine tea processed food 300mg
Fructooligosaccharide 150mg
Vitamin C 500mg
Water remaining amount

The composition of the present invention has a high sleep effect and can be used as an oral preparation, so the industrial utility of the present invention is high.

Claims (3)

An oral composition containing theanine and a fructooligosaccharide (excluding liquid dairy products and foods containing gamma-aminobutyric acid, taurine, 5-hydroxytryptophan, mineral substances and vitamins). The oral composition according to claim 1, which is in the form of a tablet, capsule, powder, granule, or liquid. 3. The oral composition according to claim 1 or 2, which is a sleep composition used for sleeping soundly.