JP2018012681A - Oral composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an oral composition having high sound sleep effect.SOLUTION: An oral composition contains theanine, and a specific component, such as at least one plant material selected from barley, sweet potato, oats, rice, rooibos, puerh, jasmine, and Terminalia.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a composition used for sleep, and more particularly to an oral composition containing theanine and certain other ingredients.

  In modern society, autonomic nerves are disturbed and insomnia is often caused by various factors such as lifestyle changes and increased stress. Insomnia has various effects such as reduced concentration, work efficiency, tiredness such as fatigue and dullness, physical condition, depression, etc .. It has been demanded.

  As a composition for such sleep, for example, lavender steam distilled water (see Patent Document 1), a composition containing hastoside (see Patent Document 2), or a composition containing verbenaline (see Patent Document 3) ) Has been proposed.

JP 2006-104073 A JP 2005-154111 A JP 2005-154310 A

  An object of the present invention is to provide a composition having a high sleep effect.

  As a result of earnest investigation and research on the further functional improvement of theanine having an effect of sleep, the present inventors have found that a higher sleep effect can be obtained by combining theanine and a specific component, thereby completing the present invention. It came to.

That is, the present invention relates to an oral composition comprising theanine and at least one other component selected from the group consisting of the following (a) to (b).
(A) at least one plant material selected from barley, sweet potato, oats, rice, rooibos, puer, jasmine and terminaria (b) at least one functional material selected from lactic acid bacteria and fructooligosaccharides

  Moreover, this invention relates to the functional food composition for sleep which mix | blended the at least 1 sort (s) of other component chosen from the group which consists of theanine as an active ingredient selected from the group which consists of said (a)-(b).

  The present invention relates to a functional food composition for promoting sleep well that contains at least one other component selected from the group consisting of (a) to (b) above, which contains theanine as an active ingredient.

  The present invention comprises a theanine and at least one other component selected from the group consisting of the above (a) to (b), and is used for a sleep that suppresses the inflow of Ca ions into nerve cells. The present invention relates to a functional food composition.

  The present invention relates to a functional food composition for sleep which contains at least one other component selected from the group consisting of the above (a) to (b) that promotes the suppression of Ca ion inflow into neurons of theanine. .

  The present invention contains a theanine and at least one other component selected from the group consisting of the above (a) to (b), and a functional food for sleep that binds to a glutamate receptor of a nerve cell Relates to the composition.

  Furthermore, the present invention provides a functional food composition for sleep which contains at least one other component selected from the group consisting of the above (a) and (b) that promotes the binding of theanine to the glutamate receptor of nerve cells. About.

  The present invention relates to an oral composition containing theanine, wherein at least one other component selected from the group consisting of (a) to (b) above is added, and a functional food composition for sleep is produced. Regarding the method.

  The oral composition of the present invention can obtain a high sleep effect by suppressing nerve excitement.

It is a figure which shows the result of the intracellular Ca fluorescence intensity change ratio at the time of applying the oral composition (theanine + other component) of this invention to the primary rat cerebral cortex origin nerve cell.

  The composition of the present invention contains theanine and at least one component selected from the group consisting of the following (a) to (b) (hereinafter sometimes referred to as other components). The components (a) to (b) used together with theanine may be used singly (for example, only component (a)) or may be used in combination of two or more. When two or more other components are used in combination, it is preferable to combine the other components having a high synergistic effect with theanine.

  The oral composition of the present invention increases the inhibitory neurotransmitter and suppresses the action of the excitatory neurotransmitter by effectively combining theanine and other components, effectively suppressing the nerve excitement, and suppressing excessive excitement. Suppressing, you can get a sleep and relaxation effect.

[Theanine]
The theanine in the oral composition of the present invention is preferably L-theanine, and the production method thereof is not particularly limited, such as those obtained by extraction from plants, those obtained by chemical synthesis, and those obtained by fermentation. Theanine is commercially available as a reagent, food additive, etc., and a commercially available product can be used.

[Other ingredients]
(A) Plant material In the oral composition of the present invention, it is preferable to use at least one plant material selected from barley, sweet potato, oats, rice, rooibos, puer, jasmine, and terminia together with theanine.

  These plant materials may be any part of the plant such as leaves, stems, roots, flowers, fruits, trunks, branches, etc. In addition to the plants themselves, their dried products, pulverized products, juices, extracts, etc. The processed plant product can be used. Examples of the pulverized product include powder and granules. The juice or extract may be liquid, but can also be used as a paste or dry powder. The extract can be obtained by extraction using an appropriate solvent, and as the solvent, for example, water (warm water, hot water), ethanol, hydrous ethanol can be used. As the plant material, commercially available products may be used.

(barley)
Barley is a plant belonging to the genus Baraceae native to Central Asia, and Nijo barley, Rojo barley and the like can be used. As a site | part used as a plant raw material of this invention, a stem and a leaf are preferable and a young leaf is more preferable. Young leaf powder obtained by drying and pulverizing young leaves and extracts from young leaves are particularly preferred.

(Gansu)
Sweet potato is a plant belonging to the convolvulaceae family and is generally called sweet potato. The sweet potato varieties are not particularly limited, and examples include suiou, joy white, koganesengan, white yutaka, sweet potato, and ayamurasaki. As a site | part used as a plant raw material of this invention, a stem and a leaf are preferable and a young leaf is more preferable. Young leaf powder obtained by drying and pulverizing young leaves and extracts from young leaves are particularly preferred.

(Oats)
Oats are a plant belonging to the genus Oataceae that originates from Patagonia in Chile, and its scientific name is Avena sativa. The parts used as the plant material of the present invention are preferably ears, stems and leaves, and more preferably dry powders obtained by drying and finely pulverizing ears and leaves and extracts from ears and leaves.

(Rice)
Rice is a plant belonging to the genus Gramineae. As the plant material of the present invention, rice (brown rice) is preferable, and rice milk produced from rice is particularly preferable. There is no restriction | limiting in particular as a kind of rice, A japonica seed | species, an indica seed | species, etc. can be mentioned.

(Terminalia)
Examples of terminaria include Terminalia bellirica (belerica), Terminalia catappa, Terminalia tomentosa, Terminalia citrina, Terminalia phellocarpa, Terminalia copelandii, Terminalia brassi, Terminalia ivorensis, Terminalia superba, Terminalia arjuna, Terminalia chebula, etc. Of these, Terminal bellirica (belerica) is preferable. As a part used as a plant material of the present invention, a fruit is preferable. A dry powder obtained by drying and pulverizing a fruit, and an extract from the fruit are particularly preferable.

(Rooibos)
Rooibos is a plant belonging to the genus Aspartus of the legume family, and leaves and branches that are usually used as tea are preferred as the plant material in the present invention. Dry powder obtained by drying and finely pulverizing leaves and branches, and extracts from leaves and branches are particularly preferred.

(Puer)
Pual is a kind of Chinese tea (black tea) originating in Yunnan Province of the People's Republic of China. There are fresh tea and mature tea, and the plant material in the present invention is preferably leaves that are usually used as tea. A dry powder obtained by drying and finely pulverizing leaves and an extract from the leaves are particularly preferable.

(jasmine)
Jasmine is a plant belonging to the family Asteraceae, and as a plant material in the present invention, a flower usually used as tea is preferable. A dry powder obtained by drying and pulverizing flowers and an extract from flowers are particularly preferred.
(B) Functional material In the oral composition of the present invention, it is preferable to use at least one functional material selected from lactic acid bacteria and fructooligosaccharides together with theanine.

(Lactic acid bacteria)
Lactic acid bacteria include Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium mongoliense, Lactbacillus brevis, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus buchlus Lactobacillus buch curvatus, Lactobacillus halivaticus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacilus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus ), Enterococcus faesium (sometimes called Streptococcus faesium), Streptococcus thermophilus, Lactococcus lactis (sometimes called Streptococcus lactis) E.g. Leuconostoc mesenteroides, Leuconostoc oenos, Pediococcus acidilactici, Pediococcus pentosaceus, Staphylococcus carnosus, Staphylococcus xylosus, Tetragenococcus halophilus, Bacillus coagulans, Bacillus mesentericus, etc. casei, Lactobacillus plantarum, Lactobacilus paracasei, Lactobacillus plantarum, Lactobacillus fermentum, Lactobacilus paracasei, Enterococcus faecalis (Streptococcus faecalis), Enterococcus faesium (Streptococcus faesium), Streptococcus thermophilus, lacteptococcus thermophilus, lacteptococcus thermophilus, Lactobacillus plantarum These lactic acid bacteria can be used individually by 1 type or in combination of 2 or more types. According to the dosage form and quality of the composition of the present invention, for example, those having properties such as heat resistance, acid resistance, sugar resistance, salt resistance, and spore resistance can be appropriately selected. The method for obtaining lactic acid bacteria is not particularly limited, and for example, lactic acid bacteria isolated from foods such as yogurt and vegetables and commercially available products can be used.

  These other components function as an accelerator for the sleep effect of theanine.

  Examples of the oral composition of the present invention include, for example, pharmaceuticals (including quasi-drugs), functional foods for which indications of efficacy have been approved by predetermined institutions such as foods for specified health use, nutritional functional foods, functional labeling foods, etc. And so-called health foods, general foods, food additives, feeds and the like. The oral composition of the present invention can be taken regardless of gender or age if it is a person who cares about the above effects. In addition, it is desirable to take 30 minutes to 2 hours before going to bed.

  The oral composition of the present invention can be used as a sleeping composition used for sleeping, and such a sleeping composition contains theanine and other predetermined components and is used as a product in terms of being used for sleeping. As long as it can be distinguished from the above, there is no particular limitation. For example, the indication of the ability to sleep in any of the main body, packaging, instructions, and promotional material of the product according to the present invention is included in the scope of the present invention. In addition, the composition for restful sleep of this invention is not restricted to the component (theanine and predetermined other component) in this invention displayed as an active ingredient of restful sleep on the product packaging etc. For example, the active ingredient may not be specified, theanine and a predetermined other component may be displayed as the active ingredient, or only theanine may be displayed as the active ingredient.

  Specifically, the composition for sleeping of the present invention includes pharmaceuticals (including quasi-drugs) and so-called health foods, and in so-called health foods, “enhance the quality of sleep”, “feeling of fatigue when waking up” "Reducing sleepiness", "Reducing the feeling of fatigue when waking up the next morning" Examples are those that display "helps to recover feeling", "supports good night sleep", "supports a healthy sleep", "supports a good night sleep", and the like.

  Examples of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, caplets , Gel, chewable, stick, and the like. Among these, a tablet, capsule, powder, granule, and liquid form are particularly preferable. Specifically, supplements, container-packed beverages filled in PET bottles, cans, bottles, instant beverages that are dissolved in water (hot water), milk, fruit juice, green juice, etc., and food additives Can be illustrated. These are preferable in that they can be easily taken at the time of eating and the like, and can enhance palatability.

  The contents of theanine and other components (components of the present invention) in the oral composition of the present invention may be appropriately contained within the range where the effects are exerted.

  In general, when the oral composition of the present invention is a pharmaceutical product or a supplement, the component of the present invention is preferably contained in an amount of 0.1 to 100% by mass in terms of dry mass, and 1 to 85% by mass. % Is more preferable, and 5-70 mass% is further more preferable.

  When the oral composition of the present invention is a packaged beverage, the component of the present invention is preferably contained in an amount of 0.0001 to 30% by mass in terms of dry mass, and 0.001 to 25% by mass is contained. More preferably, it is more preferably 0.01 to 20% by mass.

  Moreover, when the oral composition of this invention is an instant drink, it is preferable that the component of this invention is contained 0.001-80 mass% of the whole in conversion of dry mass, 0.005-70 mass% It is more preferably contained, and further preferably 0.1 to 60% by mass.

  In order to exhibit the effect of the present invention more effectively, it is preferable that the component of the present invention is contained in 80% or more of the whole oral composition of the present invention in terms of dry mass, and contained in 90% or more. Is more preferably 95% or more, and particularly preferably 100%.

  Although there is no restriction | limiting in particular as the intake of the oral composition of this invention, From a viewpoint of exhibiting the effect of this invention more notably, the intake of the component of this invention per day of an adult will be 50 mg / day or more. Ingestion is preferable, more preferably 100 mg / day or more, and even more preferably 150 mg / day or more. Although the upper limit in particular is not restrict | limited, For example, it is 10,000 mg / day, Preferably it is 5000 mg / day.

  The oral composition of the present invention can be stored in one container or divided into a plurality of containers such as 2 to 3 so that the daily intake becomes the above-mentioned intake.

  The blending mass ratio of theanine and other components is preferably in the range of 1: 0.001 to 1:50, more preferably in the range of 1: 0.01 to 1:40, in terms of dry mass. The range of 1: 0.1 to 1:30 is more preferable, and the range of 1: 1 to 1:20 is particularly preferable. When the mixing ratio of theanine and other components is within the above range, the effects of the present invention can be more effectively exhibited.

  The oral composition of this invention can be manufactured by a well-known method, adding components other than the component of this invention accept | permitted as oral use as needed.

  Although the pH at the time of dissolving the oral composition of this invention in water is not specifically limited, The range of pH2.0-7.0 is preferable, pH3.0-6.5 are more preferable, pH4.5-6. 5 is most preferred.

Hereinafter, the present invention will be described based on examples.
[Example 1]
1. Cell culture
(1) Cell density of 1.0 × 10 ⁴ cells / well on each well of 96-well black plate in which primary rat cerebral cortex-derived neurons (manufactured by Thermo) are coated with Poly-L-Lysine at a density of ² μg / cm ^2. Sowing.
(2) Pre-cultured for one week in a CO ₂ incubator at 37 ° C. Medium exchange was performed every 1-2 days.
(3) After removing the medium from each well, 100 μL of a test substance-containing medium prepared to a predetermined concentration in a normal medium was added and cultured for 24 hours in a CO ₂ incubator.
The normal medium used was Neurobasal medium (manufactured by Thermo) 48.875 ml plus 200 mM Glutamax ^™ (manufactured by Thermo) 125 μl and B27 Serum Free Supplement (50 ×) (manufactured by Thermo) 1 ml.

  For theanine, commercially available L-theanine was used. As other components used with L-theanine, the substances shown in Table 1 were used.

2. Evaluation of intracellular Ca

  In general, when an NMDA receptor (glutamate receptor, which is an excitatory amino acid) on the surface of a neuron is activated by an excitatory amino acid (glutamate), a large amount of Ca ion flows into the neuron, It is said that various Ca ion-dependent enzymes are activated and nerve hyperexcitation occurs. In this test, the effect of suppressing the inflow of Ca ions into nerve cells by the test substance was investigated.

Specifically, the following tests were conducted.
Intracellular Ca was measured using Calcium-Kit Fura2 (Dojindo Laboratories).
(1) After the culture for 24 hours, the medium was removed from each well, washed once with PBS (−), 100 μL of Loading Buffer was added, and cultured for 1 hour.
(2) The loading buffer was removed, washed once with PBS (−), 90 μL of recording buffer was added, and acclimated for 10 minutes in an incubator.

(3) Add 10 μL of 250 μM Glutamate to a final concentration of 25 μM, and (1) 1 minute after addition (2) Sample fluorescence intensity Ftest (340) (excitation wavelength 340 nm, fluorescence wavelength 510 nm), Ftest (380) (excitation Wavelength 380 nm, fluorescence wavelength 510 nm). Further, the fluorescence intensities Fpre (340) and Fpre (380) immediately before the addition of Glutamate were also measured in the same manner. Furthermore, after the test was completed, MnCl ₂ was added so that the final concentration was 0.33 M, and the fluorescence intensities Fblank (340) and Fblank (380) were measured in the same manner to calculate autofluorescence.
(4) Based on the measured value, the change value of the fluorescence intensity ratio was calculated from the following formula.

Change value of Δ fluorescence intensity ratio = Rtest−Rpre
・ Rtest = (Ftest (340)-Fblank (340)) / (Ftest (380)-Fblank (380))
・ Rpre = (Fpre (340)-Fblank (340)) / (Fpre (380)-Fblank (380))

  The results are shown in Table 1 and FIG. In FIG. 1, the numerical value described after the component name indicates the final concentration (μg / mL) of the other components. For example, “barley_200” in FIG. 1 indicates 200 (μg / mL). The vertical axis represents “intracellular Ca fluorescence intensity change ratio”.

For barley, dry pulverized powder of young leaves was used.
For sweet potatoes, dry crushed powder of young leaves was used.
For oats, Otsugulin manufactured by Toyo Shinyaku Co., Ltd., which is an extract from the whole above-ground grass including ears and stems and leaves, was used.
For rice (rice milk), commercially available rice milk was used.
For rooibos, commercially available rooibos tea powder was used.
For the puer, a commercially available powder of puer tea was used.
As for jasmine, commercially available jasmine tea powder was used.
As for Terminaria, a hot water extract (dried powder) of Terminaria berylica fruit was used.
For lactic acid bacteria, Bacillus coagulans was used.
Regarding fructooligosaccharides, commercially available fructooligosaccharides were used.
For mugwort, dry crushed powder of leaves was used.
As for lavender, a commercially available powder of lavender tea was used.

  As shown in Table 1 and FIG. 1, a decrease in the intracellular Ca fluorescence intensity ratio was confirmed by the combination of L-theanine and specific other components. That is, it is considered that the combination component of the present invention antagonized the stimulation of glutamic acid to the NMDA receptor and suppressed the inflow of Ca ions into nerve cells. Therefore, the composition of the present invention can suppress nerve excitement and can obtain a high sleep effect.

[Example 2] (Manufacture of tablets)
Five tablets comprising the following components were produced.

Oat dry extract powder 400mg
Fructooligosaccharide 250mg
L-Theanine 200mg
Hydroxypropylcellulose 350mg
Calcium stearate 250mg
50 mg of silicon dioxide

  The tablet is taken with water once or twice or three times a day.

[Example 3] (Manufacture of capsules)
The following mixture was enclosed in a soft capsule to produce a capsule.

L-Theanine 200mg
Terminaria Berylica powder 100mg
Lactic acid bacteria 400mg
Maltose 50mg
Silicon dioxide 10mg

  The above capsules are taken with 4 tablets once a day or divided into 2 to 4 times with water.

[Example 4] (Production of granules)
The following ingredients were mixed to produce granules (3000 mg) by a conventional method.

Barley young leaf dry extract powder 1200mg
Theanine 200mg
Sucralose 150mg
Thiamine hydrochloride 10mg
Riboflavin 10mg
Vitamin B6 5mg
Cyanocobalamin 6 5mg
Fragrance 5mg
Reduced palatinose 200mg
Calcium stearate 100mg
Hydroxypropylcellulose remainder

[Example 5] (Production of instant powder)
The following ingredients were mixed to produce an instant powder (2 g) by a conventional method.

Rooibos tea processed food 800mg
Theanine 200mg
Aspartame 150mg
Sucralose 50mg
Malic acid 10mg
Dye preparation 10mg
Fragrance 5mg
Polydextrose balance

[Example 6] (Manufacture of solution)
A liquid (200 mL) comprising the following components was produced.

Theanine 200mg
Pu'er tea processed food 250mg
Jasmine tea processed food 300mg
Fructooligosaccharide 150mg
Vitamin C 500mg
Water remaining

  Since the composition of the present invention has a high sleep effect and can be used as an oral preparation, the industrial utility of the present invention is high.

Claims (4)

An oral composition comprising theanine and at least one other component selected from the group consisting of the following (a) to (b).
(A) at least one plant material selected from barley, sweet potato, oats, rice, rooibos, puer, jasmine and terminaria (b) at least one functional material selected from lactic acid bacteria and fructooligosaccharides   The oral composition according to claim 1, which is in the form of a tablet, a capsule, a powder, a granule, or a liquid.   3. The oral composition according to claim 1 or 2, which is a composition for resting used for resting.   It is a functional food, The oral composition in any one of Claims 1-3 characterized by the above-mentioned.