JP2007063236A - Composition for improving quality of sleep - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition for improving the quality of sleep wherein food materials are employed to obtain good taste in no anxiety of excessive ingestion and the quality of sleep can be improved by ingestion of the composition at the bed time. <P>SOLUTION: The composition for improving the quality of sleep includes γ-aminobutyric acid. In other words, the composition for improving the quality of sleep includes γ-aminobutyric acid, as an active ingredient, that has the action to promote the deep sleep by lowering the deep bodily temperature with the intake at bed time. Further, the sleep quality improving composition can be used in food and beverage. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

The present invention relates to a composition for improving sleep quality, which can improve the quality of sleep by ingesting orally before going to bed, and more specifically, can promote a decrease in deep body temperature after going to bed.

In modern life, one of the problems has been a decline in work efficiency, mental power, and resistance to illness due to lack of exercise, stress and environmental pollution, and so on. High quality sleep is also important for beauty. Drugs such as sleeping pills are prescribed when symptoms such as sleep disorders are observed, but side effects such as addiction may be seen, so if it is not so serious insomnia, a slightly easier method is required. Therefore, several compositions for foods and drinks that enhance the effect of sleep have been proposed as methods for improving sleep quality that are surely effective, have no side effects, and can be easily executed.

For example, Patent Document 1 proposes a composition effective for sleep disorders containing a genus Valeriana or an extract thereof, a plant having a sedative action or an extract thereof, and γ-aminobutyric acid. Moreover, in patent document 2, the composition for sleep promotion characterized by containing theanine is proposed.

On the other hand, in recent years, deep body temperature has attracted attention as a measure for improving sleep quality. (See Non-Patent Document 1.) That is, the idea is that the deep body temperature is raised by daytime exercise, etc., and the deeper and more comfortable sleep is obtained when the difference from the deep body temperature after going to bed is larger.

None of the compositions described in the above-mentioned patent documents and the compositions for improving sleep so far were specifically evaluated at this deep body temperature.
JP 2003-183174 A International Publication WO01 / 074352 Shiro Oda et al. Hokkaido Physical Education Research, 36, p73. (2001)

In the present invention, by using food materials, taste is good, there is no worry of overdose, and can be improved by taking orally before going to bed, especially by lowering the deep body temperature during sleep Another object of the present invention is to provide a composition for improving sleep quality that promotes deep sleep.

As a result of intensive studies to achieve the above-mentioned problems, the present inventors have found that by taking γ-aminobutyric acid orally, the deep body temperature at the time of falling asleep is lowered, and the depth of sleep by polygraph analysis is increased, The present invention has been completed.

That is, the sleep quality improving composition of the present invention is characterized by containing γ-aminobutyric acid as an active ingredient.

In the sleep quality improving composition, it is preferable to promote a decrease in deep body temperature after going to bed.

According to the present invention, it is possible to provide a quality improving composition that can improve the quality of sleep by ingesting orally before going to bed without using the food material, having good taste and worrying about excessive intake. it can.

Another aspect of the invention is a food or drink comprising the sleep quality improving composition.

ADVANTAGE OF THE INVENTION According to this invention, the food / beverage products which can improve the quality of sleep can be provided by eating and drinking before going to bed, without the worry of excessive intake and taste.

According to the composition for improving sleep quality of the present invention, or a food or drink containing the sleep quality, the sleep quality is improved by lowering the deep body temperature, promoting deep sleep, preventing waking up at night, etc. Since it can be improved, it can also be used to recover from fatigue, improve beauty, improve health, and improve daytime work efficiency.

In the present invention, γ-aminobutyric acid is generally called GABA and refers to one type of non-protein amino acid widely distributed in nature. As a food component, it is usually contained in tea, vegetables, cereals, etc., and in the living body, it is produced by decarboxylation of glutamic acid, and in the human body, especially in the substantia nigra, basal ganglia, thalamus It is known that it exists at a high concentration in the lower part.

The γ-aminobutyric acid used in the present invention is not particularly limited, but γ-aminobutyric acid extracted from vegetables, fruits, cereals, etc., γ-aminobutyric acid produced by fermentation, and produced from organic synthesis. It means γ-aminobutyric acid. Especially, although it does not specifically limit, the fermentation method by lactic acid bacteria which can obtain (gamma) -aminobutyric acid in large quantities and cheaply is preferable. For example, a culture solution of lactic acid bacteria (Lactobacillus brevis (IFO12005 strain), Lactobacillus hilgardi K-3 strain (FERM P-18422), etc.) is added to a culture solution containing glutamic acid soda, glucose, baker's yeast extract, etc. And it culture | cultivates at 20-30 degreeC for 1-3 days, and this culture solution is filtered after heat-sterilization, and a filtrate is obtained. In addition, when Lactobacillus hilgardy K-3 strain (FERM P-18422) is used as a lactic acid bacterium, the content of γ-aminobutyric acid in the culture broth is about 5% by mass. It accounts for about 50%. The filtrate can be appropriately concentrated and used as it is, or further dried and used as a powder in the composition for improving sleep quality of the present invention.

Further, commercially available products “Pharmaca 20-D” and “Pharmaca 100” (manufactured by Pharma Foods Co., Ltd.) may be used.

Although the detection method of (gamma) -aminobutyric acid in the composition of this invention or food / beverage products is not specifically limited, It isolate | separates by the high performance chromatography (HPLC) using an ion exchange column, and orthophthalaldehyde (OPA). The method of detecting and quantifying with a fluorescence detector after derivatization with a post-column by) is preferred.

Although content of (gamma) -aminobutyric acid in the composition for sleep quality improvement of this invention is not specifically limited, It is preferable that 5-100 mass% of (gamma) -aminobutyric acid is included. If the content is less than 5% by mass, it may be necessary to ingest a large amount of powder or liquid in order to take an effective intake of the sleep quality improving composition, or the sleep quality improving composition may be consumed. When added to a product, it is not preferable because it is added at a high concentration.

There is no restriction | limiting in particular in the form of the composition for sleep quality improvement of this invention, A solution, powder, etc. can be selected according to a use. As a pulverization method, hot air drying, spray drying, freeze drying, or a general known pulverization method can be adopted.

In addition to the components described above, the composition for improving sleep quality of the present invention includes excipients, emulsifiers, stabilizers, sweeteners, pH adjusters, thickeners, proteins, peptides, amino acids, lipids, polysaccharides, Ingredients usually used in foods such as oligosaccharides may be included.

The composition for improving sleep quality of the present invention is not only added to foods and drinks, but also prepared in various forms according to the dosage form, and can also be used as powder (or granules), drinks, tablets, capsules and the like. .

Examples of the foods and drinks included in the present invention include solid foods such as dried foods and supplements, and liquid foods and drinks such as soft drinks, sports drinks / mineral waters, and beverages. Although it is not particularly limited as a solid food, in detail, for example, kneaded products, processed soybean products, mousse, jelly, yogurt, frozen dessert, strawberry, chocolate, gum, crackers, biscuits, cookies, cakes, bread, etc. It is done. In addition, although it is not particularly limited as liquid food and drink, in detail, for example, carbonated drinks, sports drinks, soft drinks, teas such as green tea, oolong tea, black tea, herbal tea, cocoa drinks, concentrated fruit juice, concentrated Examples include reduced juice, straight juice, fruit mix juice, fruit juice containing fruit juice, beverage containing fruit juice, fruit / vegetable mix juice, vegetable juice, mineral water, milk, and milk drink.

  The production method of the food and drink of the present invention is not particularly limited. For example, a production method in which γ-aminobutyric acid and other raw materials are mixed with powder, or a mixed solution is prepared by dissolving γ-aminobutyric acid and other raw materials in a solvent. A general method for producing food and drink, such as a production method, a production method in which the mixed solution is freeze-dried, and a production method in which the solution is spray-dried, can be applied. Ingredients other than γ-aminobutyric acid that can be used in producing the food and drink of the present invention can be appropriately selected according to the desired use unless the expression of the desired effect by γ-aminobutyric acid is inhibited. .

The addition amount of the sleep quality improving composition in the food or drink of the present invention is not particularly limited, but is preferably 0.1% by mass or more and 100% by mass or less. If it is less than 0.1% by mass, it is difficult to take γ-aminobutyric acid as an active ingredient.

The composition for improving sleep quality according to the present invention contains the γ-aminobutyric acid as an active ingredient. The effective intake varies depending on the age, sex, constitution, etc., but is usually preferably 50 mg to 500 mg in terms of γ-aminobutyric acid per bedtime. If it is 50 mg or less, the effect cannot be experienced so much. In addition, even if it is ingested as much as 3,000 mg in terms of γ-aminobutyric acid at a time, no particular adverse effect is seen, but even if it is ingested at a dose of 500 mg or more, the effect is not particularly noticeable.

In the composition for improving sleep quality of the present invention, it is preferable to lower the deep body temperature by 0.2 ° C. or more within one hour after going to bed after administration of the above effective intake. If the deep body temperature decrease is 0.2 ° C. or less, the sleep quality improvement effect is not seen. Moreover, it is not seen that deep body temperature falls normally 1.0 degreeC or more.

In the present invention, the deep body temperature refers to a body temperature close to the temperature inside the body, not the body temperature near the body surface. In view of ease of actual measurement, rectal temperature is preferable.

The safety of γ-aminobutyric acid used in the present invention is high. For example, in an acute toxicity test using mice, there is no death due to oral administration of 5 g / kg, and no abnormality is observed in the general state and body weight. Under the Food Sanitation Law, there is no limit to the amount added. In addition, unlike conventional drugs, no side effects due to γ-aminobutyric acid are observed. Therefore, according to the composition of the present invention, the quality of sleep can be improved safely and effectively.

  EXAMPLES Hereinafter, the present invention will be specifically described with reference to examples, but the present invention is not particularly limited thereto.

(Preparation of lactic acid bacteria fermented γ-aminobutyric acid high content material)
Lactic acid bacteria (Lactobacillus hillgardi K-3 strain) pre-cultured with a similar fermentation raw material solution after 50 L of a fermentation raw material aqueous solution (pH 5.0) containing each component at the ratio shown in Table 1 was sterilized by heating at 90 ° C. for 10 minutes. (FERM P-18422)) 50 mL of the culture solution was inoculated and cultured at 30 ° C. for 3 days. This fermentation broth was sterilized by heating at 90 ° C. for 10 minutes, and then filtered with a filter aid to obtain a filtrate.

Dextrin (manufactured by Sanwa Starch Co., Ltd.) was added to the obtained filtrate, dried and powdered with a spray dryer to obtain about 11 kg of lactic acid bacteria fermented γ-aminobutyric acid-rich material.

In addition, this manufacturing method is an illustration of preparation of (gamma) -aminobutyric acid, and does not limit this invention.

(Measurement of γ-aminobutyric acid concentration)
Each sample was appropriately diluted with 0.2N sodium citrate buffer (pH 2.2), and then centrifuged or filtered to remove solids, thereby preparing a measurement sample. The content of γ-aminobutyric acid was analyzed by a high performance liquid chromatograph under the following conditions in accordance with a conventional method for measuring amino acid content.

Equipment used: High performance liquid chromatograph LC-9A manufactured by Shimadzu Corporation
Column for analysis: Strong acid cation exchange resin column Shin-pack Isc-07Na type moving bed buffer solution: amino acid moving bed kit manufactured by Shimadzu Corporation Na type moving bed flow rate: 0.3 ml / min reaction layer 1: 0 0.04% sodium hypochlorite solution (0.2 ml of hypochlorous acid per 500 ml of boric acid-carbonate buffer at pH 10)
Reaction layer 2: Amino acid analysis kit OPA reagent reaction layer manufactured by Shimadzu Corporation Flow rate: 0.2 ml / min Detection: fluorescence detection Ex 348 nm, Em 450 nm

According to the above measurement method, the content of γ-aminobutyric acid in the fermented liquid after completion of fermentation described in Example 1 was 51 g / L. Moreover, the γ-aminobutyric acid content of the powdered lactic acid bacteria fermented γ-aminobutyric acid-rich material described in Example 1 was 21% by mass. Moreover, the content of γ-aminobutyric acid of “Pharmaca 100” (manufactured by Pharma Foods) was 100% by mass.

In order to investigate the quality of sleep in humans, human volunteers were collected and a sleep test was conducted.

(subject)
Five healthy men from the age of 20 to 29 were selected, and all of them were ingested with water before going to bed (control group) and with an aqueous solution containing 100 mg of γ-aminobutyric acid (GABA group). Were performed twice in total with a week or more in between.

(Method)
“Pharma Gabba 100” (manufactured by Pharma Foods Co., Ltd.) 100 mg dissolved in 500 mL of tap water (GABA group) or 500 mL of control tap water (control group) at 10 pm 60 minutes before bedtime It was. The test subject was put in a sleep capsule (Daikin Environment and Air Conditioning Technology Laboratory), and attached measurement devices (polygraph device and temperature logger (thermistor)) were attached in a usual manner from 10:30 pm, and preparation for measurement was performed. At 11:00 pm, measurement of EEG, electrocardiogram, myoelectric, electrooculogram, body surface temperature (skin temperature), and deep body temperature (rectal temperature) was started. The next day, it was woken up at 6 am and the measurement was completed. Based on the results of electroencephalogram, electrocardiogram, electromyogram and electrooculogram, polygraph analysis was performed (for details, see Jun Kamiyama "Sleep Physiology and Clinics" (published by Diagnosis and Treatment Company 2003)). An example is shown in FIG. The sleep depth is determined based on the electroencephalogram, electrocardiogram, electromyogram, and electrooculogram. If the depth stage is described using the electroencephalogram as a guide, the alpha wave (8 to 13 Hz) at the sleep depth 1 (the shallowest) Is 50% or less, sleep spindle wave appears at sleep depth 2, δ wave (2 Hz or less, 75 μV or more) is 20% or more at sleep depth 3, and δ wave is 50% or more at sleep depth 4 (deepest). Become. In the following, the sleep onset latency refers to the transition time to sleep onset, that is, the sleep depth 3 after going to bed. The deep sleep time refers to the time during which the sleep depth 3 or 4 is maintained.

(result)
First, the transition results of the deep body temperature (rectal temperature) are shown in FIG. In the GABA group, the decrease in deep body temperature during sleep was sharper and greater than that in the control group, and it was shown that the temperature dropped by about 0.3 ° C. 50 minutes after going to bed.

Next, the sleep latency of each group is shown in FIG. 3, and the deep sleep time of each group within 60 minutes after going to bed is shown in FIG.

As shown in FIG. 3 and FIG. 4, it was shown that the GABA group had a shorter time to fall asleep than the control group, and the deep sleep time at depth 3 or 4 was longer. That is, it was shown that sleep is better and deep sleep can be obtained.

  Hereinafter, the formulation example of the food / beverage products containing the composition for sleep quality improvement of this invention is given.

(Soft drink)
A soft drink containing the lactic acid bacteria fermented γ-aminobutyric acid-rich material prepared in Example 1 was prepared. That is, 1500 g of mixed isomerized sugar, 1000 g of lemon juice, 50 g of lactic acid bacteria fermented γ-aminobutyric acid high content material, 10 g of fragrance, and 10 g of calcium carbonate are mixed with the raw materials so that the total volume becomes 10 L. It was sterilized at 90 ° C. for 15 seconds to produce a soft drink containing γ-aminobutyric acid for improving sleep quality. That is, about 200 mg of γ-aminobutyric acid is contained in 200 mL of beverage.

(Capsule)
Improve sleep quality by blending each raw material to contain 90% lactic acid bacteria fermented γ-aminobutyric acid-rich material and 10% corn starch obtained in Example 1 and filling into gelatin capsules (200mg per capsule) Capsules were prepared.

(tablet)
Tableting (300 mg per tablet) after blending each raw material so as to contain 50% lactic acid bacteria fermented γ-aminobutyric acid-rich material obtained in Example 1, 18% reduced maltose, 28% crystalline cellulose, and 4% sucrose ester ) To produce tablets for improving sleep quality.

The composition for improving sleep quality of the present invention and the food and drink containing the same are made from safe and inexpensive γ-aminobutyric acid, improve the quality of sleep by ingestion before going to bed, and eventually recover from fatigue, beauty and health It can also be used to improve the efficiency of work, improve work efficiency during the day, and can be marketed as functional foods and beverages.

FIG. 4 is a diagram showing an example of polysomnogram analysis in the human volunteer sleep test described in Example 3. It is the graph which showed the average of transition of the deep body temperature (rectal temperature) in each group of the human volunteer sleep test described in Example 3. It is the graph which showed the average of sleep sleep latency (transition time to sleep sleep; minutes) in each group of the human volunteer sleep test described in Example 3. It is the graph which showed the average of the deep sleep time (sleep depth 3 and 4; min) in 60 minutes after falling asleep in each group of the human volunteer sleep test described in Example 3. FIG.

Claims (3)

A composition for improving sleep quality, comprising γ-aminobutyric acid as an active ingredient. The composition for improving sleep quality according to claim 1, which promotes a decrease in deep body temperature after going to bed. A food or drink comprising the composition for improving sleep quality according to claim 1 or 2.

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