JP7257665B2 - oral composition - Google Patents

Description

The present invention relates to an oral composition containing a digestible oligosaccharide and a given material.

Since digestible oligosaccharides have various functions, they are sometimes added to food and drink in small amounts. For example, glucosyl sucrose is used as a low cariogenic sweetener because it does not produce insoluble glucans that cause tooth decay. (See Patent Document 1).

On the other hand, digestible oligosaccharides are decomposed by digestive enzymes and absorbed as sugar in the stomach and small intestine, and therefore are not preferable from the viewpoint of diet and beauty.

Japanese Patent Publication No. 01-049471

An object of the present invention is to provide an oral composition that can enjoy the functions of digestible oligosaccharides and suppresses the digestive enzymes from decomposing the digestive oligosaccharides to suppress gastrointestinal absorption.

The present inventors have found that by blending two or more selected from vitamins, barley, lactic acid bacteria, and water-soluble dietary fiber together with digestible oligosaccharides, the decomposition of digestible oligosaccharides by digestive enzymes is suppressed. This led to the completion of the present invention. In particular, the inventors have found that even when vitamins are blended alone, the decomposition of digestible oligosaccharides by digestive enzymes is extremely effectively inhibited.

That is, the present invention is as follows.
[1] An oral composition comprising a digestible oligosaccharide and two or more ingredients selected from vitamins, barley, lactic acid bacteria, and water-soluble dietary fiber.
[2] An oral composition comprising a digestible oligosaccharide and a vitamin.

The oral composition of the present invention can enjoy the functions of digestible oligosaccharides and can suppress gastrointestinal absorption of digestible oligosaccharides.

1 is a graph showing the α-glucosidase inhibition rate when the oral composition of the present invention was applied.

The oral composition according to the first invention comprises a digestible oligosaccharide and two or more materials selected from vitamins, barley, lactic acid bacteria, and water-soluble dietary fiber (hereinafter sometimes referred to as the material of the present invention). contains. It is preferable to contain three or more kinds of materials of the present invention, and it is preferable to contain all of them. In particular, it is preferable to contain vitamins and/or barley.

An oral composition according to a second invention contains digestible oligosaccharides and vitamins. As other ingredients, it is preferable to contain at least one of barley, lactic acid bacteria, and water-soluble dietary fiber, and it is more preferable to contain two or more.

The oral composition of the present invention can enjoy the functions of digestible oligosaccharides and can suppress the absorption of such digestible oligosaccharides. That is, the oral composition of the present invention has functions such as moderate sweetness of digestible oligosaccharides, suppression of deterioration of the composition (improved shelf life), and masking of unpleasant odors, while reducing sugars that are undesirable from the viewpoint of diet and the like. Absorption can be suppressed. Therefore, the oral composition of the present invention is particularly useful for diet foods and the like.

Each material contained in the oral composition of the present invention is described below.
[Digestible oligosaccharide]
The digestible oligosaccharide in the present invention is an oligosaccharide having a trisaccharide or more that is decomposed by human digestive enzymes and absorbed in the stomach and small intestine to become energy. The number of constituent sugars of the digestible oligosaccharide that can be used in the present invention is preferably trisaccharide or more and decasaccharide or less, more preferably trisaccharide or more and octasaccharide or less, and more preferably trisaccharide or more and hexasaccharide or less. Preferably, the trisaccharide or more and tetrasaccharide or less is particularly preferable from the viewpoint of the effect. Examples of digestible oligosaccharides include malto-oligosaccharides, iso-malto-oligosaccharides, soybean oligosaccharides, glucosylsucrose, and the like, and malto-oligosaccharides are preferable because the effects of the present invention can be more enjoyed. Specific examples of malto-oligosaccharides include maltotriose, maltotetraose, maltopentaose, maltohexaose and the like, and maltotriose is particularly preferred because the effects of the present invention can be more enjoyed. These digestible oligosaccharides can be used singly or in combination of two or more.

The content of the digestible oligosaccharide is not particularly limited, but is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, more preferably 0.01% by mass in terms of dry mass with respect to the entire oral composition. % or more is more preferable, and 0.05% by mass or more is particularly preferable. Although the upper limit is not particularly limited, it is preferably 30% by mass, more preferably 10% by mass, even more preferably 5% by mass, and particularly preferably 1% by mass.

[Material of the present invention]
(vitamin)
As vitamins, various vitamins can be used, and they may be water-soluble vitamins or oil-soluble vitamins, but water-soluble vitamins are preferable because the effects of the present invention can be more enjoyed. Water-soluble vitamins include vitamin B group (vitamin B3, B5, B6, B9, B12, B13, B15, B17, etc.) and vitamin C. Among the water-soluble vitamins, B vitamins are preferable, and vitamin B6 (pyridoxine) is particularly preferable, since the effects of the present invention can be more effectively enjoyed. Among vitamin B6, pyridoxine hydrochloride is particularly preferred. Examples of oil-soluble vitamins include vitamins A, D, E, K, and the like. These vitamins can be used individually by 1 type or in combination of 2 or more types.

The content of the vitamin is not particularly limited, but is preferably 0.00001% by mass or more, preferably 0.001% by mass or more, in terms of dry mass with respect to the entire oral composition from the viewpoint of being able to enjoy the effects of the present invention more. is more preferable, 0.01% by mass or more is more preferable, and 0.03% by mass or more is particularly preferable. Although the upper limit is not particularly limited, it is preferably 30% by mass, more preferably 10% by mass, even more preferably 5% by mass, and particularly preferably 3% by mass.

The blending mass ratio of digestible oligosaccharides and vitamins is not particularly limited, but from the viewpoint of being able to enjoy the effects of the present invention more, it is preferably 1:0.001 to 1:100 in terms of dry mass. :0.01 to 1:10 is more preferable, 1:0.05 to 1:5 is more preferable, and 1:0.1 to 1:1 is particularly preferable.

(barley)
Barley (Hordeum vulgare L.) is native to Central Asia and is an annual or perennial herb belonging to the Poaceae family. The barley used in the present invention may be any barley such as two-rowed barley or six-rowed barley. Moreover, you may use any kind|variety. The site used in the present invention is preferably a leaf, and among the leaves, a green leaf is preferable, and a young leaf is particularly preferable, from the viewpoint that the effect of the present invention can be more enjoyed. Moreover, the stem may be included together with the leaves.

As for barley, processed barley can be used. Examples of pulverized materials include dry powders, shredded materials and dried materials thereof (dried shredded materials), granules, and the like. The squeezed juice or extract may be in liquid form, but may also be used in the form of paste or dry powder (squeezed juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of solvents that can be used include water (warm water, hot water), ethanol, and hydrous ethanol. In the present invention, it is particularly preferable to use a dry powder of young barley grass (green barley powder) from the viewpoint that the effects of the present invention can be further enjoyed.

The content of barley is not particularly limited, but is preferably 1% by mass or more, more preferably 5% by mass or more, based on the dry mass of the entire oral composition from the viewpoint of being able to enjoy the effects of the present invention more. 10% by mass or more is more preferable, and 30% by mass or more is particularly preferable. The upper limit is not particularly limited, but is preferably 90% by mass, more preferably 80% by mass, even more preferably 60% by mass, and particularly preferably 50% by mass.

The blending weight ratio of digestible oligosaccharides and barley is not particularly limited. : 1 to 1:1000, more preferably 1:10 to 1:300, and particularly preferably 1:50 to 1:200.

(Lactic acid bacteria)
Lactic acid bacteria in the present invention is a general term for bacteria that produce lactic acid through metabolism, and is a concept that includes bifidobacteria.乳酸菌としては、Bifidobacterium bifidum、Bifidobacterium breve、Bifidobacterium infantis、Bifidobacterium lactis、Bifidobacterium longum、Bifidobacterium adolescentis、Bifidobacterium mongoliense、Lactbacillus brevis、Lactbacillus gasseri、Lactobacillus acidophilus、Lactobacillus buchneri、Lactobacillus bulgaricus、Lactobacillus delburvecki、Lactobacillus casei、Lactobacillus crispatus、Lactobacillus Also known as curvatus, Lactobacillus halivaticus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacilus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus hilgardii, Lactobacillus reuteri, Lactobacillus straecalis fermentum ), Enterococcus faesium (sometimes called Streptococcus faesium), Streptococcus thermophilus, Lactococcus lactis (sometimes called Streptococcus lactis), Leuconostoc mesenteroides, Leuconostoc oenos, Pediococcus acidilactici, Pediococcus pentosaceus, Staphylococcus carnosus, Staphylococcus xylosus , Tetragenococcus halophilus, Bacillus coagulans, Bacillus mesentericus, etc., Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus fermentum, Enterococcus faecalis (Streptococcus faecalis) (Streptococcus faesium), Streptococcus thermophilus, Lactococcus lactis (Streptococcus lactis), Bacillus coagulans, Bacillus mesentericus are preferred. Lactic acid bacteria other than bifidobacteria can also be used. These lactic acid bacteria can be used individually by 1 type or in combination of 2 or more types. Depending on the dosage form and quality of the oral composition of the present invention, for example, those having properties such as heat resistance, acid resistance, sugar resistance, salt tolerance, and spore formation can be appropriately selected. The method for obtaining lactic acid bacteria is not particularly limited, and for example, lactic acid bacteria isolated from foods such as yogurt and vegetables, and commercially available products can be used. It is particularly preferable to use lactic acid bacteria belonging to the genus Lactobacillus from the viewpoint that the effects of the present invention can be more enjoyed.

The content of lactic acid bacteria is not particularly limited, but is preferably 0.00001% by mass or more, preferably 0.001% by mass or more, in terms of dry mass with respect to the entire oral composition from the viewpoint that the effects of the present invention can be further enjoyed. is more preferable, 0.005% by mass or more is more preferable, and 0.01% by mass or more is particularly preferable. Although the upper limit is not particularly limited, it is preferably 5% by mass, more preferably 1% by mass, still more preferably 0.5% by mass, and particularly preferably 0.1% by mass.

The blending mass ratio of digestible oligosaccharides and lactic acid bacteria is not particularly limited, but from the viewpoint of being able to enjoy the effects of the present invention more, it is preferably 1:0.001 to 1:300 in terms of dry mass. :0.01 to 1:100 is more preferable, 1:0.01 to 1:10 is more preferable, and 1:0.03 to 1:3 is particularly preferable.

(Water-soluble dietary fiber)
Water-soluble dietary fiber refers to water-soluble indigestible components (dietary fiber) contained in food that are not digested by human digestive enzymes. The water-soluble dietary fiber in the present invention is not particularly limited. , fucoidan, and derivatives thereof. These can be used individually by 1 type or in combination of 2 or more types.

In the present invention, inulin is preferred among these from the viewpoint of being able to enjoy the effects of the present invention more. Inulin is a polysaccharide contained in plants such as agave, garlic, leek, chicory, artichoke, Jerusalem artichoke, etc. These plants and those extracted from these plants can be used. Among these, inulin derived from agave is particularly preferred. Inulin can also be synthesized from sucrose by a fermentation method, and such synthetic products may be used.

The content of water-soluble dietary fiber is not particularly limited, but from the viewpoint of being able to enjoy the effects of the present invention more, the dry mass of the entire oral composition is preferably 0.01% by mass or more, and 0.1 It is more preferably at least 1% by mass, even more preferably at least 1% by mass, and particularly preferably at least 5% by mass. Although the upper limit is not particularly limited, it is preferably 90% by mass, more preferably 50% by mass, even more preferably 30% by mass, and particularly preferably 20% by mass.

The blending weight ratio of the digestible oligosaccharide and the water-soluble dietary fiber is not particularly limited, but from the viewpoint of being able to enjoy the effects of the present invention more, it is preferably 1:0.1 to 1:1000 in terms of dry weight. It is preferably from 1:3 to 1:500, even more preferably from 1:5 to 1:100, and particularly preferably from 1:10 to 1:60.

In addition to general foods, food additives, feeds, etc., the oral composition of the present invention is, for example, a food for specified health use, a food with nutrient function claims, a food with functional claims, etc. Function that has been approved by a predetermined organization for labeling efficacy It can be used as a so-called health food such as sex food, or as a drug (including quasi-drugs).

Although the oral composition of the present invention contains digestible oligosaccharides, the absorption of such digestible oligosaccharides into the gastrointestinal tract is suppressed based on the α-glucosidase inhibitory action of the material of the present invention. It is useful as a food or the like.

That is, the oral composition of the present invention can be used as an oral diet composition used for dieting (anti-obesity) such as so-called health foods and pharmaceuticals. It is not particularly limited as long as it contains sugar and the material of the present invention and can be distinguished from other products as a product in terms of being used for dieting. The scope of the present invention includes any packaging, instruction manual, or promotional material that indicates that it has a diet function. The oral composition for dieting of the present invention is not limited to one in which the material of the present invention is indicated as an active ingredient for dieting on the packaging of the product. For example, an active ingredient may not be specified, or a specified material may be indicated as an active ingredient.

Specific examples of the oral composition for dieting of the present invention include "for those who are concerned about their weight", "increases metabolism", "helps reduce body fat in obese people", and "metabolizes fat". Increases the ability to exercise, Supports style, For those who are concerned about body fat, For those who are slightly overweight, For those who are concerned about their weight (BMI), Weight and abdominal fat (visceral fat and subcutaneous fat)", "reduce waist circumference", "lose weight", "become ideal body", "diet", "slim", "be healthy", "support health", etc. The so-called health food displayed can be exemplified.

In addition, since the oral composition of the present invention has an α-glucosidase inhibitory action, it can be expected to have an inhibitory effect on digestion and absorption of sugar, an inhibitory effect on blood sugar level elevation, and an effect on diabetes and diabetes complications prevention. Therefore, the oral composition of the present invention can be used as a composition for inhibiting α-glucosidase, a composition for suppressing digestion and absorption of sugar, a composition for suppressing elevation of blood sugar level, and a composition for preventing diabetes and diabetes complications. .

Forms of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. shape, gel shape, chewable shape, stick shape, and the like. Among these, tablets, capsules, powders, granules, and liquids are preferred from the viewpoint that the effects of the present invention can be enjoyed more. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant beverages (powder beverages) for dissolving in water (hot water), milk, fruit juice, green juice, etc. , food additives can be exemplified. These are preferable in that they are easy to drink with meals and can improve palatability. Moreover, when the present invention is a powdered drink, the oral composition of the present invention is particularly preferably a powdered drink because it is characterized by being easily dispersed in water.

The oral composition of the present invention can be produced by a known method by adding other ingredients other than the ingredients of the present invention, if necessary. Other ingredients other than the ingredients of the present invention include, for example, minerals such as calcium, magnesium, phosphorus and iron; sulfur-containing compounds contained in taurine, garlic and the like; flavanoids or flavonoids such as hesperidin and quercetin; Proteins; peptides; amino acids; animal oils and fats; vegetable oils and fats;

The present invention will be described below based on examples.
[Example 1]
(Test substance)
As a vitamin, vitamin B6 (pyridoxine hydrochloride) was used.
As barley, young barley powder produced by the method described in Japanese Patent No. 3277181 was used. Specifically, harvested young barley leaves are blanched with hot water, dried until the moisture content is 5% by weight or less, coarsely pulverized, and then heated at 110° C. or higher to finely pulverize. We used young barley leaf powder produced by
As lactic acid bacteria, lactic acid bacteria (Lactobacillus gasseri) adjusted to 50 mg/mL and incubated at 70°C for 30 minutes were used.
As a water-soluble dietary fiber, agave inulin extracted from agave stems, dried and pulverized was used.

[Absorption inhibition test of digestible oligosaccharide]
Using maltotriose as a digestible oligosaccharide, a digestive oligosaccharide absorption test was carried out. Specifically, we tested how much the degradation of maltotriose by α-glucosidase was inhibited. Details are below.

(1) Each test substance was weighed into a 1.5 mL tube, 56 mM maleic acid buffer (solvent) was added to 50 mg/mL, and the tube was vortexed for 60 minutes.
(2) After centrifugation with a centrifuge (10,000 rpm, room temperature, 5 minutes), the supernatant was collected and used as a test substance solution.
(3) Each test substance solution was mixed at the ratio shown in Table 1 below to prepare a sample solution.

(4) 15 μL of each sample was added to a new 1.5 mL tube. Solvent was used as a control.
(5) Add 15 μL of the crude enzyme solution to the test tube, add 15 μL of the crude enzyme solution inactivated by heating at 98° C. for 2 min to the blank tube, vortex lightly, flush, and cool to 37° C. , 5 min.

(6) After adding 90 µL of the substrate solution to the test and blank tubes, they were vortexed and incubated at 37°C for 30 minutes.
(7) Each tube was heated at 98° C. for 2 minutes and cooled on ice to obtain a reaction solution.
(8) Add 40 μL of each reaction solution and 300 μL of the coloring agent attached to the commercial kit “Glucose CII Test Wako” to a 96-well plate, incubate at 37° C. for 5 minutes, and then measure the absorbance at 505 nm with a plate reader.
(9) Using the measured absorbance, the inhibition rate was calculated by the following formula.

Inhibition rate (%)
= (1 - [(Sample _test - Sample _blank ) / (Control _test - Control _blank )]) x 100

1.56 mM maltotriose was used as the substrate solution. As the enzyme solution, 100 mg of rat intestinal acetone powder and 900 μL of 56 mM maleic acid buffer were suspended, homogenized on ice for 2 minutes, centrifuged at 3,000 rpm, 4° C. for 10 minutes, and the supernatant was diluted 4 times with 56 mM maleic acid buffer. A diluted one was used.

The results (inhibition rate %) are shown in FIG.

As shown in FIG. 1, the composition of Example 1 containing vitamins exhibited extremely high α-glucosidase inhibitory activity. In addition, the compositions of Examples 2 to 5 containing two or more of the materials of the present invention exhibited a synergistic improvement in the α-glucosidase inhibition rate compared to the case of using one of the materials of the present invention.

[Formulation example]
An oral composition of the present invention was manufactured according to the following formulation.

Raw materials were mixed so as to have the blending amounts shown in Table 2 below to obtain a powdered beverage (Prescription Example 1). The powdered beverage was designed to be divided into 3 g packets and suspended in 100 mL of water for drinking. The resulting powdered beverage has the effects of the present invention, and is a powdered beverage with good dispersibility and excellent taste.

After mixing each component shown in Table 3 below, a tableting machine was used to produce tablets each having 250 mg and a tablet diameter of 8 mm (Prescription Example 2). The resulting tablet has the effects of the present invention and is easy to swallow with good slipperiness.

The mixtures shown in Table 4 below were encapsulated in hard capsules to produce capsules (300 mg) (formulation example 3). Five hard capsules thus obtained are ingested per day. The obtained hard capsule has the effects of the present invention.

After mixing the components shown in Table 5 below, the mixture was sterilized at 90°C for 10 minutes to produce a beverage (formulation example 4). The resulting liquid formulation has the effects of the present invention, is excellent in flavor and taste, and is a drink that is smooth down the throat.

The oral composition of the present invention is industrially useful because it can be used as a so-called health food or the like.

Claims (2)

An oral composition containing maltotriose, vitamin B6, lactic acid bacteria, water-soluble dietary fiber, and barley, characterized by containing vitamin B6 (excluding barley-derived one). Dietary oral compositions in tablet form (excluding those containing horse oil). Maltotriose, vitamin B6 (excluding those derived from barley) , lactic acid bacteria, water - soluble dietary fiber, and barley are mixed, and then tableted. However, excluding those containing horse oil) manufacturing method.

Images