JP7037161B2 - Oral composition - Google Patents

Description

Application of Article 30, Paragraph 2 of the Patent Law Publication start date: March 7, 2016 Publication address: http: // www. caa. go. jp / foods / docks / ichiran. xls https: // www. fld. caa. go. jp / caaks / cssc02 / pdf / A244-ippan. pdf https: // www. fld. caa. go. jp / caaks / cssc02 / pdf / A244-kihon. pdf https: // www. fld. caa. go. jp / caaks / cssc02 / pdf / A244-kinou. pdf https: // www. fld. caa. go. jp / caaks / cssc02 / pdf / A244-anden. pdf

The present invention relates to compositions used for restful sleep, and more particularly to oral compositions containing theanine and certain other ingredients.

In modern society, the autonomic nerves are disturbed due to various factors such as changes in lifestyle and increased stress, and insomnia is often annoying. Insomnia has various effects such as decreased concentration and work efficiency, fatigue such as tiredness and tiredness, physical illness, depression, etc., so from the viewpoint of social life and health, good quality and peaceful sleep is possible. It has been demanded.

Examples of the composition for such a good night's sleep include steam distilled water of lavender (see Patent Document 1), a composition containing hastatoside (see Patent Document 2), and a composition containing verbenaline (see Patent Document 3). ) Has been proposed.

Japanese Unexamined Patent Publication No. 2006-104073 Japanese Unexamined Patent Publication No. 2005-154311 Japanese Unexamined Patent Publication No. 2005-154310

An object of the present invention is to provide a composition having a high sleep effect.

As a result of diligent research and research on further functional improvement of theanine having a restful sleep effect, the present inventors have found that a higher sleep sleep effect can be obtained by combining theanine with a specific component, and complete the present invention. It came to.

That is, the present invention relates to an oral composition comprising theanine and at least one other component selected from the group consisting of the following (a) to (b).
(A) At least one plant material selected from barley, sweet potato, oat, rice, rooibos, puer, jasmine, and terminaria (b) At least one functional material selected from lactic acid bacteria and fructooligosaccharides

The present invention also relates to a functional food composition for a good night's sleep, which comprises at least one other ingredient selected from the group consisting of the above (a) to (b), which is characterized by containing theanine as an active ingredient.

The present invention relates to a functional food composition for promoting a good night's sleep, which comprises at least one other ingredient selected from the group consisting of the above (a) to (b), which contains theanine as an active ingredient.

The present invention is characterized by containing theanine and at least one other component selected from the group consisting of the above (a) to (b) for a good night's sleep, which suppresses the influx of Ca ions into nerve cells. Regarding functional food compositions.

The present invention relates to a functional food composition for sleep that contains at least one other component selected from the group consisting of the above (a) to (b) that promotes suppression of Ca ion influx into nerve cells of theanine. ..

The present invention is a functional food for sleep that binds to glutamate receptors of nerve cells, which comprises theanine and at least one other component selected from the group consisting of the above (a) to (b). Regarding the composition.

Further, the present invention is a functional food composition for sleep, which comprises at least one other component selected from the group consisting of the above (a) to (b), which promotes the binding of theanine to a glutamate receptor in nerve cells. Regarding.

INDUSTRIAL APPLICABILITY The present invention is a production of a functional food composition for a good night's sleep, which comprises an oral composition containing theanine and at least one other component selected from the group consisting of the above (a) to (b). Regarding the method.

The oral composition of the present invention can obtain a high sleep effect by suppressing nerve excitement.

It is a figure which shows the result of the intracellular Ca fluorescence intensity change ratio when the oral composition (theanine + other component) of this invention was applied to the nerve cell derived from the primary rat cerebral cortex.

The composition of the present invention is characterized by containing theanine and at least one component (hereinafter, may be referred to as another component) selected from the group consisting of the following (a) to (b). The components (a) to (b) used together with theanine may be used alone (for example, only the component (a)) or in combination of two or more. When two or more other components are used in combination, it is preferable to combine theanine and other components having a high synergistic effect.

The oral composition of the present invention increases inhibitory neurotransmitters and suppresses the action of excitatory neurotransmitters by a combination of theanine and other components, effectively suppresses nerve excitement, and causes excessive excitement. It suppresses and can obtain a good sleep effect and a relaxing effect.

[Theanine]
As the theanine in the oral composition of the present invention, L-theanine is preferable, and the production method thereof may be limited, such as those obtained by extraction from plants, those obtained by chemical synthesis, and those obtained by fermentation. Theanine is commercially available as a reagent, a food additive, or the like, and a commercially available product can be used.

[Other ingredients]
(A) Plant material In the oral composition of the present invention, it is preferable to use at least one plant material selected from barley, sweet potato, oat barley, rice, rooibos, puer, jasmine, and terminaria together with theanine.

These plant materials may be any part of the plant such as leaves, stems, roots, flowers, fruits, trunks, branches, etc., and in addition to the plant itself, its dried products, crushed products, juices, extracts, etc. Plant processed products can be used. Examples of the crushed product include powder, granules and the like. The juice or extract may be liquid, but it can also be used as a paste or a dry powder. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, water (hot water, hot water), ethanol, or hydrous ethanol can be used. As the plant material, a commercially available one may be used.

(barley)
Barley is a plant of the genus Hordeum of the Gramineae family native to Central Asia, and two-row barley, six-row barley and the like can be used. As the site used as the plant material of the present invention, stems and leaves are preferable, and young leaves are more preferable. Young leaf powder obtained by drying and finely pulverizing young leaves and an extract from young leaves are particularly preferable.

(Sweet potato)
Sweet potato refers to a plant belonging to the family Convolvulaceae, and is generally called sweet potato. The variety of sweet potato is not particularly limited, and examples thereof include sweet potato, Joy White, Koganesengan, Shiroyutaka, sweet potato starch, and Ayamurasaki. As the site used as the plant material of the present invention, stems and leaves are preferable, and young leaves are more preferable. Young leaf powder obtained by drying and finely pulverizing young leaves and an extract from young leaves are particularly preferable.

(Oat)
Oat is a plant of the genus Oat of the Gramineae family, which originates in Patagonia, Chile, and its scientific name is Avena sativa. As the site used as the plant material of the present invention, ears, stems and leaves are preferable, and dry powder obtained by drying and finely pulverizing ears and foliage, and extracts from ears and foliage are more preferable.

(Rice)
Rice is a plant of the genus Oryza in the family Gramineae. As the plant material of the present invention, rice (brown rice) is preferable, and rice milk produced from rice is particularly preferable. The type of rice is not particularly limited, and examples thereof include japonica rice and indica rice.

(Terminaria)
Examples of the terminaria include Terminalia bellirica (belerica), Terminalia catappa, Terminalia tomentosa, Terminalia citrina, Terminalia phellocarpa, Terminalia copelandii, Terminalia brassi, Terminalia ivorensis, Terminalia superba, Terminalia arjuna, Terminalia chebula and the like. Of these, Terminalalia bellirica (belerica) is preferable. Fruits are preferable as the site used as the plant material of the present invention. Dry powder obtained by drying and finely grinding the fruit and an extract from the fruit are particularly preferable.

(Rooibos)
Rooibos is a plant belonging to the genus Cape Gorses of the family Leguminosae, and as the plant material in the present invention, leaves and branches usually used as tea are preferable. Dry powder obtained by drying and finely pulverizing leaves and branches, and extracts from leaves and branches are particularly preferable.

(Puar)
Pual is a kind of Chinese tea (black tea) originating from Yunnan Province of the People's Republic of China, and includes raw tea and mature tea. As the plant material in the present invention, leaves usually used as tea are preferable. Dry powder obtained by drying and finely grinding the leaves and an extract from the leaves are particularly preferable.

(jasmine)
Jasmine is a plant belonging to the family Oleaceae, and as the plant material in the present invention, flowers usually used as tea are preferable. Dry powder obtained by drying and finely pulverizing flowers and an extract from flowers are particularly preferable.
(B) Functional Material In the oral composition of the present invention, it is preferable to use at least one functional material selected from lactic acid bacteria and fructooligosaccharide together with theanine.

(Lactic acid bacteria)
The lactic acid bacteria, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium mongoliense, Lactbacillus brevis, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus buchneri, Lactobacillus bulgaricus, Lactobacillus delburvecki, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus there curvatus, Lactobacillus halivaticus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacilus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus hilgardii, Lactobacillus reuteri, Lactobacillus fermentum, also referred to as Enterococcus faecalis (Streptococcus faecalis ), Enterococcus faesium (sometimes called Streptococcus faesium), Streptococcus thermophilus, Lactococcus lactis (sometimes called Streptococcus lactis), Leuconostoc mesenteroides, Leuconostoc oenos, Pediococcus acidilactici, Pediococcus xylosus, Tetragenococcus halophilus, Bacillus coagulans, Bacillus mesentericus, etc., Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus (Streptococcus faecalis), Enterococcus faesium (Streptococcus faesium), Streptococcus thermophilus, Lactococcus lactis (Streptococcus lactis), Bacillus coagulans, Bacillus mesentericus are preferred. These lactic acid bacteria can be used alone or in combination of two or more. Depending on the dosage form and quality of the composition of the present invention, for example, those having properties such as heat resistance, acid resistance, sugar resistance, salt resistance and spore resistance can be appropriately selected. The method for obtaining lactic acid bacteria is not particularly limited, and for example, lactic acid bacteria isolated from foods such as yogurt and vegetables and commercially available products can be used.

These other ingredients function as promoters of theanine's restful sleep effect.

The oral composition of the present invention includes, for example, functional foods whose efficacy has been approved by a predetermined organization such as pharmaceuticals (including non-medicinal products), foods for specified health use, foods with nutritional function, foods with functional claims, etc. So-called health foods such as, general foods, food additives, feeds and the like can be mentioned. The oral composition of the present invention can be ingested by a person who is concerned about the above effects regardless of gender or age. It is also desirable to take it 30 minutes to 2 hours before bedtime.

The oral composition of the present invention can be used as a composition for a good night's sleep, and such a composition for a good night's sleep contains theanine and other predetermined components, and is another product as a product in that it is used for a good night's sleep. It is not particularly limited as long as it can be distinguished from. For example, the scope of the present invention includes any of the main body, packaging, instruction manual, and advertising material of the product according to the present invention indicating that the product has a good sleep ability. The composition for restful sleep of the present invention is not limited to the one in which the components of the combination in the present invention (theanine and predetermined other components) are displayed as the active ingredient of restful sleep on the packaging of the product or the like. For example, the active ingredient may not be specified, theanine and a predetermined other ingredient may be labeled as the active ingredient, or only theanine may be labeled as the active ingredient.

Specific examples of the composition for sleep of the present invention include pharmaceuticals (including non-medicinal products) and so-called health foods. In so-called health foods, "improve the quality of sleep" and "feeling tired when waking up". "Reduces sleepiness", "Reduces fatigue (feeling of tiredness and tiredness) when waking up the next morning", "Brings a healthy sleep", "Refreshing awakening", "Reduces and fatigue of drowsiness when waking up" Examples of those displaying "useful for recovery of feeling", "supporting good quality sleep at night", "supporting healthy sleep", "supporting healthy sleep at night", etc. can be exemplified.

The form of the oral composition of the present invention includes, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. The shape, gel shape, chewable shape, stick shape and the like can be mentioned. Among these, tablet-like, capsule-like, powder-like, granular, and liquid forms are particularly preferable. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant beverages for dissolving in water (hot water), milk, fruit juice, green juice, etc., and food additives. Can be exemplified. These are preferable in that they are easy to drink at the time of meals and can enhance the palatability.

The content of theanine and other components (components of the present invention) in the oral composition of the present invention may be appropriately contained within the range in which the effect is exhibited.

Generally, when the oral composition of the present invention is a pharmaceutical product or a supplement, it is preferable that the component of the present invention is contained in an amount of 0.1 to 100% by mass in terms of dry mass, and 1 to 85% by mass. It is more preferable that it is contained in%, and it is further preferable that it is contained in an amount of 5 to 70% by mass.

When the oral composition of the present invention is a packaged beverage, the component of the present invention is preferably contained in an amount of 0.0001 to 30% by mass, preferably 0.001 to 25% by mass, in terms of dry weight. It is more preferable that it is contained, and it is further preferable that it is contained in an amount of 0.01 to 20% by mass.

When the oral composition of the present invention is an instant beverage, the components of the present invention are preferably contained in an amount of 0.001 to 80% by mass, preferably 0.005 to 70% by mass, in terms of dry mass. It is more preferably contained, and further preferably 0.1 to 60% by mass.

In order to exert the effect of the present invention more effectively, it is preferable that the component of the present invention is contained in an amount of 80% or more, and 90% or more, of the entire oral composition of the present invention in terms of dry mass. Is more preferable, 95% or more is further preferable, and 100% is particularly preferable.

The intake amount of the oral composition of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, the daily intake of the component of the present invention for an adult is 50 mg / day or more. It is preferable to take it so as to be 100 mg / day or more, and it is further preferable to take it so as to be 150 mg / day or more. The upper limit is not particularly limited, but is, for example, 10,000 mg / day, preferably 5000 mg / day.

The oral composition of the present invention can be contained in one container or divided into a plurality of containers, for example, 2 to 3 so that the daily intake becomes the above-mentioned intake, and contained as one day's worth.

The compounding mass ratio of theanine and other components is preferably in the range of 1: 0.001 to 1:50, more preferably in the range of 1: 0.01 to 1:40 in terms of dry mass. , The range of 1: 0.1 to 1:30 is more preferable, and the range of 1: 1 to 1:20 is particularly preferable. When the blending ratio of theanine and other components is within the above range, the effect of the present invention can be more effectively exhibited.

The oral composition of the present invention can be produced by a known method by adding components other than the components of the present invention that are acceptable for oral use, if necessary.

The pH when the oral composition of the present invention is dissolved in water is not particularly limited, but is preferably in the range of pH 2.0 to 7.0, more preferably pH 3.0 to 6.5, and pH 4.5 to 6. 5 is the most preferable.

Hereinafter, the present invention will be described based on examples.
[Example 1]
1. 1. Cell culture
(1) Cell density of 1.0 × 10 ⁴ cells / well in each well of 96-well black plate coated with Poly-L-Lycine at a density of 2 μg / cm ² of primary rat cerebral cortex-derived neurons (manufactured by Thermo). Was sown in.
(2) The cells were cultured at 37 ° C. in a CO ₂ incubator one week before. Medium exchange was performed every 1 to 2 days.
(3) After removing the medium from each well, 100 μL of the test substance-containing medium prepared to a predetermined concentration in a normal medium was added, and the cells were cultured in a CO ₂ incubator for 24 hours.
As a normal medium, 48.875 ml of Neurobasal medium (manufactured by Thermo), 125 μl of 200 mM Glutamax ^TM (manufactured by Thermo) and 1 ml of B27 Serum Free Supplement (50 ×) (manufactured by Thermo) were added.

For theanine, commercially available L-theanine was used. As the other components used together with L-theanine, the substances shown in Table 1 were used.

2. 2. Evaluation of intracellular Ca

Generally, when the NMDA receptor (glutamate receptor, which is an excitatory amino acid) on the surface of a nerve cell is activated by the excitatory amino acid (glutamic acid), a large amount of Ca ions flow into the nerve cell, and then It is said that various Ca ion-dependent enzymes are activated to cause nerve hyperexcitability. In this test, the effect of the test substance on suppressing the influx of Ca ions into nerve cells was investigated.

Specifically, the following tests were conducted.
Intracellular Ca was measured using Calcium-Kit Fura2 (manufactured by Dojin Chemical Research Institute).
(1) After culturing for the above 24 hours, the medium was removed from each well, washed once with PBS (−), 100 μL of Loading Buffer was added, and the cells were cultured for 1 hour.
(2) The Loading Buffer was removed, washed once with PBS (−), 90 μL of Recording Buffer was added, and the mixture was acclimatized in an incubator for 10 minutes.

(3) Add 10 μL of 250 μM Glutamate so that the final concentration is 25 μM, and 1 minute after the addition, (2) Fluorescence intensity Ftest (340) (excitation wavelength 340 nm, fluorescence wavelength 510 nm), Ftest (380) (excitation) of the sample. The wavelength was 380 nm and the fluorescence wavelength was 510 nm). In addition, the fluorescence intensities Fpre (340) and Fpre (380) immediately before the addition of Glutamic acid were also measured in the same manner. Further, after the test was completed, MnCl ₂ was added so as to have a final concentration of 0.33 M, and the fluorescence intensities Fblank (340) and Fblank (380) were also measured in the same manner to calculate autofluorescence.
(4) Based on the measured values, the change value of the fluorescence intensity ratio was calculated from the following formula.

Δ Change in fluorescence intensity ratio = Test-Rpre
・ Rtest = (Ftest (340) --Fblank (340)) / (Ftest (380) --Fblank (380))
・ Rpre = (Fpre (340) --Fblank (340)) / (Fpre (380) --Fblank (380))

The results are shown in Table 1 and FIG. In FIG. 1, the numerical value described after the component name indicates the final concentration (μg / mL) of the other component. For example, "barley_200" in FIG. 1 indicates 200 (μg / mL). The vertical axis shows the "intracellular Ca fluorescence intensity change ratio".

For barley, dried and crushed young leaves were used.
For sweet potatoes, dried and crushed powder of young leaves was used.
For oats, oats manufactured by Toyo Shinyaku Co., Ltd., which is an extract from the whole above-ground grass including ears and foliage, was used.
For rice (rice milk), commercially available rice milk was used.
For rooibos, commercially available crushed powder of rooibos tea was used.
For Pu'er, crushed powder of commercially available Pu'er tea was used.
For jasmine, commercially available crushed powder of jasmine tea was used.
For Terminaria, a hot water extract (dry powder) of Terminaria beryrica fruit was used.
For lactic acid bacteria, Bacillus coagulans was used.
As the fructooligosaccharide, a commercially available fructooligosaccharide was used.
For mugwort, dried and crushed leaves were used.
For lavender, commercially available crushed powder of lavender tea was used.

As shown in Table 1 and FIG. 1, it was confirmed that the combination of L-theanine and a specific other component reduced the intracellular Ca fluorescence intensity ratio. That is, it is considered that the combination component of the present invention antagonized the stimulation of glutamate to the NMDA receptor and suppressed the influx of Ca ions into nerve cells. Therefore, the composition of the present invention can suppress nerve hyperexcitability and can obtain a high sleep effect.

[Example 2] (Manufacturing of tablets)
Five tablets consisting of the following ingredients were manufactured.

Oat dried extract powder 400mg
Fructooligosaccharide 250mg
L-Theanine 200mg
Hydroxypropyl cellulose 350mg
Calcium stearate 250 mg
Silicon dioxide 50mg

The above tablets should be taken with water once or divided into two or three times a day.

[Example 3] (Production of capsule)
The following mixture was encapsulated in soft capsules to produce capsules.

L-Theanine 200mg
Terminaria beryrica powder 100 mg
Lactic acid bacteria 400mg
Maltose 50mg
Silicon dioxide 10 mg

The above capsules are taken in 4 capsules once a day or in 2 to 4 divided doses with water.

[Example 4] (Production of granules)
The following components were mixed to prepare granules (3000 mg) by a conventional method.

Barley young leaf dried extract powder 1200 mg
Theanine 200mg
Sucralose 150 mg
Thiamine hydrochloride 10 mg
Riboflavin 10 mg
Vitamin B6 5 mg
Cyanocobalamin 65 mg
Fragrance 5 mg
Reduced palatinose 200 mg
Calcium stearate 100 mg
Hirodoxypropyl Cellulose Remaining

[Example 5] (Manufacturing of instant powder)
The following ingredients were mixed to produce an instant powder (2 g) by a conventional method.

Rooibos tea processed food 800mg
Theanine 200mg
Aspartame 150mg
Sucralose 50 mg
Malic acid 10 mg
Dye preparation 10 mg
Fragrance 5 mg
Polydextrose remnants

[Example 6] (Manufacturing of liquid preparation)
A liquid preparation (200 mL) consisting of the following components was produced.

Theanine 200mg
Pu'er tea processed food 250mg
Jasmine tea processed food 300mg
Fructooligosaccharide 150mg
Vitamin C 500mg
Remaining water

Since the composition of the present invention has a high sleep-resting effect and can be used as an oral preparation, the industrial usefulness of the present invention is high.

Claims (5)

An oral composition for a good night's sleep containing theanine as an active ingredient, which further contains terminaria . An oral composition for a good night's sleep containing theanine as an active ingredient, which further contains oats ( excluding those containing matcha ). An oral composition for a good night's sleep containing theanine as an active ingredient, which further comprises at least one plant material selected from young barley leaves and sweet potatoes (provided that it contains green tea or matcha). except). An oral composition for a good night's sleep containing theanine as an active ingredient, which is characterized by further containing rice milk (excluding those containing corn tea and nilgiri black tea). An oral composition for a good night's sleep containing theanine as an active ingredient, which is characterized by further containing Bacillus coagulan s (excluding those containing corn tea and nilgiri black tea).

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