JP2023162913A - disinfectant composition - Google Patents
disinfectant composition Download PDFInfo
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- JP2023162913A JP2023162913A JP2022073626A JP2022073626A JP2023162913A JP 2023162913 A JP2023162913 A JP 2023162913A JP 2022073626 A JP2022073626 A JP 2022073626A JP 2022073626 A JP2022073626 A JP 2022073626A JP 2023162913 A JP2023162913 A JP 2023162913A
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Abstract
Description
本発明は、消毒剤組成物、消毒剤組成物含浸物品、ウイルス不活化方法、殺菌方法及び消毒剤組成物の製造方法に関する。 The present invention relates to a disinfectant composition, an article impregnated with a disinfectant composition, a method for inactivating viruses, a method for sterilization, and a method for producing a disinfectant composition.
ウイルス性の急性胃腸炎や下痢症は、介護施設や病院、学校等での集団感染として確認されるだけでなく、調理施設やレストラン等においても汚染された食品を介した食中毒による集団感染としても数多く報告されている。食中毒の原因ウイルスとして、ノロウイルスやサポウイルス、ロタウイルス等のエンベロープを持たないウイルスが知られており、これらのウイルスは小児や高齢者へ感染すると重篤な症状を引き起こすことがある。 Viral acute gastroenteritis and diarrhea are not only confirmed as mass infections in nursing homes, hospitals, schools, etc., but also as mass infections due to food poisoning through contaminated food in cooking facilities, restaurants, etc. Many cases have been reported. Non-enveloped viruses such as norovirus, sapovirus, and rotavirus are known to cause food poisoning, and these viruses can cause serious symptoms when infected to children and the elderly.
特に、ノロウイルスは集団感染を引き起こすウイルスとして問題視されている。感染経路は、生カキ等の貝類を食することによる経口感染がよく知られているが、家庭や公共施設等において、汚染食品に触れた人の手指や調理器具上、患者の嘔吐物や糞便中、あるいは患者の周辺環境に存在しているウイルスからの二次感染も数多く報告されている。特にこのような二次感染は、集団感染や感染拡大につながり、患者数の増加の大きな原因となっている。 In particular, norovirus is viewed as a problem as a virus that causes mass infections. It is well known that the route of infection is oral infection through eating raw oysters and other shellfish, but in homes and public facilities, infection can occur on the fingers or cooking utensils of people who have come into contact with contaminated food, or on patients' vomit or feces. There have also been many reports of secondary infections from viruses existing inside or in the patient's surrounding environment. In particular, such secondary infections lead to mass infections and spread of infection, and are a major cause of the increase in the number of patients.
ノロウイルスを不活化する方法としては、エタノールを主成分とする消毒剤組成物を用いる方法が知られている。
特許文献１には、（ａ）エタノール又はイソプロピルアルコール４０～９０％、（ｂ）乳酸０．１～２％、（ｃ）クエン酸０．０１～２％、（ｄ）亜鉛イオンを遊離する亜鉛含有化合物０．００１～０．１％を含み、ｐＨが２．５～５．０である消毒剤が開示されている。
特許文献２には、（Ａ）低級アルコール３５～７５質量％、（Ｂ）有機酸及びそのアルカリ金属塩、及び／又は、無機酸及びそのアルカリ金属塩０．０５～１０質量％、（Ｃ）グリセリン脂肪酸エステルのような非イオン界面活性剤０．０５～５質量％を含み、ｐＨが８～１２である、便座用除菌洗浄剤組成物及びこれを含有する除菌洗浄材、並びにこれらを用いた除菌洗浄方法が開示されている。
特許文献３には、（Ａ）低級アルコールを３５～７５質量％、（Ｂ）無機アルカリ性物質０．０５～１０質量％、（Ｃ）グリセリン脂肪酸エステルのような非イオン界面活性剤０．０５～５質量％を含み、ｐＨが９．５以上である、殺菌消毒剤組成物及びそれを用いた殺菌消毒方法が開示されている。
特許文献４には、エタノール、炭酸塩を０．１０重量％を超えて８．００重量％未満及び酸剤を含む、ウイルス不活性化剤が開示されている。
特許文献５には、（Ａ）一価の低級アルコール、（Ｂ）無機アルカリ性物質、並びに（Ｃ）多価アルコール又は糖類を含む、殺菌消毒剤組成物及び殺菌消毒方法が開示されている。
As a method for inactivating norovirus, a method using a disinfectant composition containing ethanol as a main component is known.
Patent Document 1 describes (a) 40 to 90% ethanol or isopropyl alcohol, (b) 0.1 to 2% lactic acid, (c) 0.01 to 2% citric acid, and (d) zinc that liberates zinc ions. Disinfectants are disclosed that contain 0.001-0.1% of compounds and have a pH of 2.5-5.0.
Patent Document 2 describes (A) lower alcohol 35 to 75% by mass, (B) organic acid and its alkali metal salt, and/or inorganic acid and its alkali metal salt 0.05 to 10% by mass, (C) A toilet seat disinfectant cleaning composition containing 0.05 to 5% by mass of a nonionic surfactant such as glycerin fatty acid ester and having a pH of 8 to 12, a disinfectant cleaning material containing the same, and The sterilization cleaning method used is disclosed.
Patent Document 3 describes (A) 35 to 75% by mass of a lower alcohol, (B) 0.05 to 10% by mass of an inorganic alkaline substance, and (C) 0.05 to 0.05% of a nonionic surfactant such as glycerin fatty acid ester. A sterilizing disinfectant composition containing 5% by mass and having a pH of 9.5 or higher and a sterilizing method using the same are disclosed.
Patent Document 4 discloses a virus inactivator containing ethanol, carbonate in an amount of more than 0.10% by weight and less than 8.00% by weight, and an acid agent.
Patent Document 5 discloses a sterilizing disinfectant composition and a sterilizing method containing (A) a monovalent lower alcohol, (B) an inorganic alkaline substance, and (C) a polyhydric alcohol or saccharide.
なお、これらの特許文献に記載されるウイルス不活化試験においては、ノロウイルスの代替ウイルスであるネコカリシウイルスが用いられている。アメリカ合衆国環境保護庁（ＥＰＡ）のノロウイルス試験法では、ネコカリシウイルスＦ９株が代替ウイルスとして一般的に用いられており、抗ウイルス試験や実験等の場合には、日本国内外でノロウイルスの代替ウイルスとして主にネコカリシウイルス（ＦＣＶ）が用いられているのが現状である。 Note that in the virus inactivation tests described in these patent documents, feline calicivirus, which is a substitute virus for norovirus, is used. In the U.S. Environmental Protection Agency (EPA) norovirus testing method, feline calicivirus strain F9 is generally used as a substitute virus, and in the case of antiviral tests and experiments, it is used as a substitute virus for norovirus in Japan and overseas. Currently, feline calicivirus (FCV) is mainly used.
近年、ヒト小腸オルガノイドｈＳＩＯ：ｈｕｍａｎ Ｓｍａｌｌ ＩｎｔｅｓｔｉｎｅＯｒｇａｎｏｉｄ（ヒト小腸エンテロイドｈＳＩＥ：ｈｕｍａｎ Ｓｍａｌｌ Ｉｎｔｅｓｔｉｎｅ Ｅｎｔｅｒｏｉｄ）二次元培養法を用いて、インビトロにおいてヒトノロウイルス（ＨＮＶ）を安定的に増殖させることに成功したとの報告を受け（非特許文献１）、当該培養系を利用してヒトノロウイルスの不活化を直接評価することが試みられた結果、被検薬剤で処理したウイルス溶液に牛血清アルブミン溶液を添加した後、超遠心分離し、得られた沈殿物をｈＳＩＯに感染させることにより、薬剤によるヒトノロウイルス不活化効果を安定的に評価できることが見出されている（特許文献６）。
また、これを用いて、ヒトノロウイルス不活化剤の評価を行うことが、特許文献７に開示されている。そして、特許文献７には、エタノール４０～９０質量％、アルカリ剤又は塩基性アミノ酸０．０１～２質量％を含有するヒトノロウイルス不活化剤が開示されている。
Recently, it has been reported that human small intestine organoid hSIO (human Small Intestine Organoid) (hSIE) two-dimensional culture method was used to successfully grow human norovirus (HNV) stably in vitro. (Non-Patent Document 1), an attempt was made to directly evaluate the inactivation of human norovirus using this culture system. It has been found that by centrifuging and infecting hSIO with the obtained precipitate, the human norovirus inactivation effect of a drug can be stably evaluated (Patent Document 6).
Furthermore, Patent Document 7 discloses that a human norovirus inactivating agent is evaluated using this. Patent Document 7 discloses a human norovirus inactivating agent containing 40 to 90% by mass of ethanol and 0.01 to 2% by mass of an alkaline agent or basic amino acid.
一般的にエタノール濃度が高ければ高いほどウイルス不活化効果が高まることが知られている。また、エタノールをアルカリ性にシフトさせることでウイルス不活化効果を高めることができることが知られている。そして、消毒剤組成物が、食品を扱う設備や食品等に用いられることを勘案するとアルカリ剤として食品添加物を用いることが望ましく、アルカリ剤としては炭酸塩が一般的である。
しかしながら、高濃度のエタノールと炭酸塩を併用し、かつ配合成分の溶解や保存安定性を損なわないようにすると消毒される対象表面が白化する等の課題が生じる。そのため既存の炭酸塩を含むアルコール系消毒剤においては、エタノール濃度が５０質量％程度に抑えられている場合が多い。
本発明は、上記課題を鑑みてなされた発明であり、炭酸塩を含みエタノール濃度が６０質量％以上の消毒剤組成物でも、消毒される対象表面に白残りを生じることなく、十分な消毒効果を有する消毒剤組成物、消毒剤組成物含浸物品、ウイルス不活化方法、殺菌方法及び消毒剤組成物の製造方法を提供する。
It is generally known that the higher the ethanol concentration, the higher the virus inactivation effect. It is also known that the virus inactivation effect can be enhanced by shifting ethanol to alkalinity. Considering that the disinfectant composition is used for food processing equipment, food, etc., it is desirable to use food additives as the alkaline agent, and carbonates are generally used as the alkaline agent.
However, when high-concentration ethanol and carbonate are used in combination without impairing the dissolution or storage stability of the ingredients, problems arise such as whitening of the surface to be disinfected. Therefore, in existing alcohol disinfectants containing carbonate, the ethanol concentration is often suppressed to about 50% by mass.
The present invention was made in view of the above-mentioned problems, and even a disinfectant composition containing carbonate and having an ethanol concentration of 60% by mass or more has sufficient disinfection effect without leaving any white residue on the surface to be disinfected. Disinfectant compositions, articles impregnated with disinfectant compositions, virus inactivation methods, sterilization methods, and methods for producing disinfectant compositions are provided.
本発明は、（Ａ）エタノールを６０質量％以上８０質量％以下、（Ｂ）炭酸カリウムを０．３０質量％以上０．８０質量％以下、（Ｃ）コハク酸、リンゴ酸、フマル酸、アジピン酸及びそれらの塩から選ばれる１種以上を０．０６質量％以上０．３０質量％以下、並びに（Ｄ）クエン酸及び／又はその塩を０．０２質量％以上０．１０質量％以下含有し、ｐＨが２５℃で９．５以上である、消毒剤組成物に関する。 The present invention includes (A) ethanol from 60% by mass to 80% by mass, (B) potassium carbonate from 0.30% by mass to 0.80% by mass, (C) succinic acid, malic acid, fumaric acid, and adipine. Contains 0.06% by mass or more and 0.30% by mass or less of one or more types selected from acids and their salts, and (D) 0.02% by mass or more and 0.10% by mass or less of citric acid and/or its salts. The present invention relates to a disinfectant composition having a pH of 9.5 or higher at 25°C.
また、本発明は、上記消毒剤組成物が基体に含浸されている、消毒剤組成物含浸物品に関する。 The present invention also relates to an article impregnated with a disinfectant composition, wherein a substrate is impregnated with the disinfectant composition described above.
また、本発明は、上記消毒剤組成物を、ウイルスが存在する対象表面に接触させる、ウイルス不活化方法に関する。 The present invention also relates to a method for inactivating viruses, which comprises bringing the disinfectant composition into contact with a target surface on which viruses are present.
また、本発明は、上記消毒剤組成物を、微生物が存在する対象表面に接触させる、殺菌方法に関する。 The present invention also relates to a sterilization method in which the disinfectant composition is brought into contact with a target surface where microorganisms are present.
また、本発明は、（Ａ）エタノール〔以下、（Ａ）成分という〕、（Ｂ）炭酸カリウム〔以下、（Ｂ）成分という〕、（Ｃ）コハク酸、リンゴ酸、フマル酸、アジピン酸及びそれらの塩から選ばれる１種以上〔以下、（Ｃ）成分という〕、並びに（Ｄ）クエン酸及び／又はその塩〔以下、（Ｄ）成分という〕を混合し、
（Ａ）成分を６０質量％以上８０質量％以下、（Ｂ）成分を０．３０質量％以上０．８０質量％以下、（Ｃ）成分を０．０６質量％以上０．３０質量％以下、（Ｄ）成分を０．０２質量％以上０．１０質量％以下含有し、ｐＨが２５℃で９．５以上である消毒剤組成物を製造する、消毒剤組成物の製造方法に関する。
The present invention also provides (A) ethanol [hereinafter referred to as component (A)], (B) potassium carbonate [hereinafter referred to as component (B)], (C) succinic acid, malic acid, fumaric acid, adipic acid, and Mixing one or more selected from these salts [hereinafter referred to as component (C)] and (D) citric acid and/or its salt [hereinafter referred to as component (D)],
(A) component is 60% by mass or more and 80% by mass or less, (B) component is 0.30% by mass or more and 0.80% by mass or less, (C) component is 0.06% by mass or more and 0.30% by mass or less, The present invention relates to a method for producing a disinfectant composition that contains component (D) at 0.02% by mass or more and 0.10% by mass or less and has a pH of 9.5 or more at 25°C.
本発明によれば、炭酸塩を含みエタノール濃度が６０質量％以上の消毒剤組成物でも、消毒される対象表面に白残りを生じることなく、十分な消毒効果を有する消毒剤組成物、消毒剤組成物含浸物品、ウイルス不活化方法、殺菌方法及び消毒剤組成物の製造方法が提供される。 According to the present invention, there is provided a disinfectant composition and a disinfectant that have a sufficient disinfecting effect without leaving a white residue on the surface to be disinfected even when the disinfectant composition contains carbonate and has an ethanol concentration of 60% by mass or more. Composition-impregnated articles, methods of virus inactivation, methods of sterilization, and methods of making disinfectant compositions are provided.
発明者らは、上記課題を解決するべく、種々の成分を検討したところ、消毒剤組成物中に、コハク酸、リンゴ酸、フマル酸及びアジピン酸及びそれらの塩から選ばれる１種以上〔（Ｃ）成分である〕とクエン酸及び／又はその塩〔（Ｄ）成分である〕とを含めることによって、エタノール濃度が６０％質量以上で炭酸塩を含むアルコール系消毒剤組成物であっても、消毒される対象表面に白残りを生じることなく、十分な消毒効果を有する組成物が得られることを見出し、本発明を完成するに至った。この（Ｃ）成分及び（Ｄ）成分を所定量含む組成物が、消毒される対象表面での白残りの発生を抑制できることは、当業者にとって予測されないことである。
なお、本発明において、消毒とは、ウイルスの不活化、除ウイルス、抗ウイルス、細菌や真菌等の微生物の殺菌、除菌、及び抗菌から選ばれる１以上を含む。
よって、本発明の消毒剤組成物は、ウイルス不活化剤組成物、除ウイルス剤組成物、抗ウイルス剤組成物、殺菌剤組成物、除菌剤組成物、抗菌剤組成物の１以上の組成物であってよい。
In order to solve the above problem, the inventors investigated various components and found that one or more components selected from succinic acid, malic acid, fumaric acid, adipic acid, and salts thereof [( By including citric acid and/or its salt [which is component (C)] and citric acid and/or its salt [which is component (D)], even if the alcohol-based disinfectant composition has an ethanol concentration of 60% by mass or more and contains carbonate. It was discovered that a composition having a sufficient disinfecting effect can be obtained without leaving any white residue on the surface to be disinfected, and the present invention was completed. It is unexpected for those skilled in the art that a composition containing a predetermined amount of component (C) and component (D) can suppress the generation of white residue on the surface to be disinfected.
In the present invention, disinfection includes one or more selected from virus inactivation, virus removal, antivirus, sterilization of microorganisms such as bacteria and fungi, sterilization, and antibacterial.
Therefore, the disinfectant composition of the present invention includes one or more of the following compositions: a virus inactivator composition, a virus removal composition, an antiviral composition, a disinfectant composition, a disinfectant composition, and an antibacterial composition. It can be a thing.
＜消毒剤組成物＞
本発明の消毒剤組成物は、（Ａ）エタノール〔以下、（Ａ）成分という〕、（Ｂ）炭酸カリウム〔以下、（Ｂ）成分という〕、（Ｃ）コハク酸、リンゴ酸、フマル酸、アジピン酸及びそれらの塩から選ばれる１種以上〔以下、（Ｃ）成分という〕、並びに（Ｄ）クエン酸及び／又はその塩〔以下、（Ｄ）成分という〕を含有し、ｐＨが２５℃で９．５以上である。
本発明の消毒剤組成物は、（Ａ）成分、（Ｂ）成分、（Ｃ）成分及び（Ｄ）成分が配合されてなる、消毒剤組成物であってよい。
<Disinfectant composition>
The disinfectant composition of the present invention includes (A) ethanol [hereinafter referred to as component (A)], (B) potassium carbonate [hereinafter referred to as component (B)], (C) succinic acid, malic acid, fumaric acid, Contains one or more types selected from adipic acid and their salts [hereinafter referred to as component (C)], and (D) citric acid and/or its salts [hereinafter referred to as component (D)], and has a pH of 25°C. is 9.5 or higher.
The disinfectant composition of the present invention may be a disinfectant composition in which component (A), component (B), component (C), and component (D) are blended.
（Ａ）成分の原料エタノールとしては、発酵エタノール、合成エタノール、バイオエタノール、精製エタノール等の通常のものを使用可能であるが、消毒用途に応じた安全性を担保できる品質のものを採用することが好適である。 As raw material ethanol for component (A), normal ethanol such as fermented ethanol, synthetic ethanol, bioethanol, purified ethanol, etc. can be used, but ethanol of a quality that can ensure safety according to the disinfection purpose should be used. is suitable.
（Ｃ）成分は、コハク酸、リンゴ酸、フマル酸、アジピン酸及びそれらの塩から選ばれる１種以上である。
（Ｃ）成分は、コストの観点から、好ましくはコハク酸、リンゴ酸、フマル酸及びそれらの塩から選ばれる１種以上、より好ましくはコハク酸、リンゴ酸及びそれらの塩から選ばれる１種以上である。
本発明の消毒剤組成物は、（Ｃ）成分の配合成分として、ｐＨの調整しやすさの観点から、コハク酸、リンゴ酸、フマル酸及びアジピン酸から選ばれる１種以上、好ましくはコハク酸、リンゴ酸及びフマル酸から選ばれる１種以上、より好ましくはコハク酸及びリンゴ酸から選ばれる１種以上を含むことが好ましい。
Component (C) is one or more selected from succinic acid, malic acid, fumaric acid, adipic acid, and salts thereof.
From the viewpoint of cost, component (C) is preferably one or more selected from succinic acid, malic acid, fumaric acid, and salts thereof, more preferably one or more selected from succinic acid, malic acid, and salts thereof. It is.
The disinfectant composition of the present invention contains at least one selected from succinic acid, malic acid, fumaric acid, and adipic acid, preferably succinic acid, from the viewpoint of ease of adjusting the pH, as the component (C). , malic acid and fumaric acid, more preferably one or more selected from succinic acid and malic acid.
（Ｃ）成分の塩は、ナトリウム、カリウム等のアルカリ金属塩、アンモニウム塩が挙げられ、食品添加物として使用されうる観点から、好ましくはアルカリ金属塩、より好ましくはナトリウム塩である。 Examples of the salt of component (C) include alkali metal salts such as sodium and potassium salts, and ammonium salts, and from the viewpoint of being usable as a food additive, preferably alkali metal salts, more preferably sodium salts.
（Ｄ）成分は、クエン酸及び／又はその塩である。（Ｄ）成分の塩は、ナトリウム、カリウム等のアルカリ金属塩、アンモニウム塩が挙げられ、食品添加物として使用されうる観点から、好ましくはアルカリ金属塩、より好ましくはカリウム塩である。
本発明の消毒剤組成物は、（Ｄ）成分の配合成分として、クエン酸を含むことが好ましい。
Component (D) is citric acid and/or a salt thereof. Examples of the salt of component (D) include alkali metal salts such as sodium and potassium salts, and ammonium salts, and from the viewpoint of being usable as a food additive, alkali metal salts are preferred, and potassium salts are more preferred.
The disinfectant composition of the present invention preferably contains citric acid as a component of component (D).
＜組成及びその他成分＞
本発明の消毒剤組成物は、（Ａ）成分を、十分なウイルス不活化及び／又は殺菌等の消毒効果を得る観点から、６０質量％以上、好ましくは６２質量％以上、より好ましくは６４質量％以上、そして、配合成分の溶解性及び保存安定性の観点から、８０質量％以下、好ましくは７５質量％以下、より好ましくは７２質量％以下含有する。
<Composition and other ingredients>
The disinfectant composition of the present invention contains component (A) in an amount of 60% by mass or more, preferably 62% by mass or more, more preferably 64% by mass, from the viewpoint of obtaining sufficient disinfection effects such as virus inactivation and/or sterilization. % or more, and from the viewpoint of solubility and storage stability of the blended components, it is contained in an amount of 80% by mass or less, preferably 75% by mass or less, more preferably 72% by mass or less.
本発明の消毒剤組成物は、（Ｂ）成分を、十分なウイルス不活化及び／又は殺菌等の消毒効果を得る観点から、０．３０質量％以上、好ましくは０．３２質量％以上、より好ましくは０．３３質量％以上、そして、配合成分の溶解性及び保存安定性の観点から、０．８０質量％以下、好ましくは０．７５質量％以下、より好ましくは０．７２質量％以下含有する。 The disinfectant composition of the present invention contains component (B) in an amount of 0.30% by mass or more, preferably 0.32% by mass or more, from the viewpoint of obtaining sufficient disinfection effects such as virus inactivation and/or sterilization. The content is preferably 0.33% by mass or more, and from the viewpoint of solubility and storage stability of the blended components, 0.80% by mass or less, preferably 0.75% by mass or less, and more preferably 0.72% by mass or less. do.
本発明の消毒剤組成物は、（Ｃ）成分を、配合成分の溶解性及び保存安定性の観点から、０．０６質量％以上、好ましくは０．０７質量％以上、より好ましくは０．０８質量％以上、そして、消毒される対象表面の白残り抑制の観点から、０．３０質量％以下、好ましくは０．２７質量％以下、より好ましくは０．２５質量％以下含有する。なお、本発明において、（Ｃ）成分の含有量に関する規定は、（Ｃ）成分を酸型の構造に仮定した酸換算値を用いる。 The disinfectant composition of the present invention contains component (C) in an amount of 0.06% by mass or more, preferably 0.07% by mass or more, more preferably 0.08% by mass, from the viewpoint of solubility and storage stability of the compounded components. The content is 0.30% by mass or less, preferably 0.27% by mass or less, and more preferably 0.25% by mass or less from the viewpoint of suppressing white residue on the surface of the object to be disinfected. In the present invention, the content of component (C) is defined using an acid equivalent value assuming that component (C) has an acid type structure.
本発明の消毒剤組成物は、（Ｄ）成分を、消毒される対象表面の白残り抑制の観点から、０．０２質量％以上、好ましくは０．０３質量％以上、より好ましくは０．０４質量％以上、そして、配合成分の溶解性及び保存安定性の観点から、０．１０質量％以下、好ましくは０．０９質量％以下、より好ましくは０．０８質量％以下含有する。なお、本発明において、（Ｄ）成分の含有量に関する規定は、（Ｄ）成分を酸型の構造に仮定した酸換算値を用いる。 The disinfectant composition of the present invention contains component (D) in an amount of 0.02% by mass or more, preferably 0.03% by mass or more, more preferably 0.04% by mass, from the viewpoint of suppressing white residue on the surface of the object to be disinfected. The content is at least 0.10% by mass, preferably at most 0.09% by mass, and more preferably at most 0.08% by mass from the viewpoint of solubility and storage stability of the blended components. In the present invention, the content of component (D) is specified using an acid equivalent value assuming that component (D) has an acid type structure.
本発明の消毒剤組成物は、（Ｃ）成分及び（Ｄ）成分を上記割合で含むことで、当業者が予期されないような、消毒される対象表面での白残りの発生を抑制することができる。 By containing the component (C) and the component (D) in the above proportions, the disinfectant composition of the present invention can suppress the occurrence of white residue on the surface to be disinfected, which would not be expected by a person skilled in the art. can.
本発明の消毒剤組成物において、（Ｃ）成分の含有量と（Ｄ）成分の含有量の質量比（Ｃ）／（Ｄ）は、配合成分の溶解性及び保存安定性の観点から、好ましくは２．０以上、より好ましくは２．３以上、更に好ましくは２．５以上、そして、消毒される対象表面の白残り抑制の観点から、好ましくは７．０以下、より好ましくは６．０以下、更に好ましくは５．０以下、より更に好ましくは４．０以下、より更に好ましくは３．５以下である。 In the disinfectant composition of the present invention, the mass ratio (C)/(D) between the content of component (C) and the content of component (D) is preferably is 2.0 or more, more preferably 2.3 or more, even more preferably 2.5 or more, and from the viewpoint of suppressing white residue on the surface to be disinfected, preferably 7.0 or less, more preferably 6.0 Below, it is more preferably 5.0 or less, even more preferably 4.0 or less, even more preferably 3.5 or less.
本発明の消毒剤組成物は、任意に、（Ｅ）（Ｃ）成分及び（Ｄ）成分を除く酸剤又はその塩〔以下、（Ｅ）成分という〕を含有することができる。
（Ｅ）成分としては、例えば、酒石酸、乳酸、グルコン酸等の酸剤又はその塩が挙げられる。
（Ｅ）成分の塩は、ナトリウム、カリウム等のアルカリ金属塩、アンモニウム塩が挙げられ、食品添加物として使用されうる観点から、好ましくはアルカリ金属塩、より好ましくはカリウム塩である。
本発明の消毒剤組成物は、消毒される対象表面の白残り抑制や保存安定性の観点から、（Ｃ）成分と（Ｄ）成分の合計含有量と、（Ｃ）成分、（Ｄ）成分及び（Ｅ）成分の合計含有量との質量比［（Ｃ）＋（Ｄ）］／［（Ｃ）＋（Ｄ）＋（Ｅ）］が、好ましくは０．８０以上、より好ましくは０．９０以上、そして、好ましくは１．００以下である。本発明の消毒剤組成物は、この質量比［（Ｃ）＋（Ｄ）］／［（Ｃ）＋（Ｄ）＋（Ｅ）］が、１．００、すなわち、該消毒剤組成物に含まれる酸剤が、（Ｃ）成分と（Ｄ）成分のみである消毒剤組成物であってよい。
The disinfectant composition of the present invention may optionally contain an acid agent or a salt thereof (hereinafter referred to as component (E)) other than components (E), (C), and (D).
Examples of the component (E) include acid agents such as tartaric acid, lactic acid, and gluconic acid, or salts thereof.
Examples of the salt of component (E) include alkali metal salts such as sodium and potassium salts, and ammonium salts, and from the viewpoint of being usable as a food additive, preferably alkali metal salts, more preferably potassium salts.
The disinfectant composition of the present invention has a total content of components (C) and (D), and components (C) and (D) from the viewpoint of suppressing white residue on the surface to be disinfected and storage stability. The mass ratio [(C)+(D)]/[(C)+(D)+(E)] of the total content of component (E) is preferably 0.80 or more, more preferably 0.80 or more. It is 90 or more, and preferably 1.00 or less. The disinfectant composition of the present invention has a mass ratio [(C)+(D)]/[(C)+(D)+(E)] of 1.00, that is, the disinfectant composition contains The disinfectant composition may include only components (C) and (D) as acid agents.
本発明の消毒剤組成物は、配合成分の溶解性及び保存安定性を損なわない範囲で、任意に、（Ｆ）（Ｂ）成分以外のアルカリ剤〔以下、（Ｆ）成分という〕を含むことができる。（Ｆ）成分を配合することによりエタノールのウイルス不活化及び／又は殺菌等の消毒効果が向上する。
（Ｆ）成分としては、炭酸塩（ただし、炭酸カリウムを除く）、炭酸水素塩、アルカリ金属の水酸化物（例えば水酸化ナトリウム、水酸化カリウム等）及びアルカリ土類金属の水酸化物（例えば水酸化マグネシウム、水酸化カルシウム等）等から選ばれる１種以上が挙げられる。このうち、好ましくは炭酸塩及び炭酸水素塩から選ばれる１種以上（ただし、炭酸カリウムを除く）である。
また、塩としては、ナトリウム塩、カリウム塩等のアルカリ金属塩、マグネシウムやカルシウム等のアルカリ土類金属塩又はアンモニウム塩等が挙げられる。
The disinfectant composition of the present invention may optionally contain an alkaline agent other than components (F) and (B) [hereinafter referred to as component (F)] to the extent that the solubility and storage stability of the ingredients are not impaired. I can do it. By blending component (F), the disinfection effect of ethanol such as virus inactivation and/or sterilization is improved.
Component (F) includes carbonates (excluding potassium carbonate), hydrogen carbonates, alkali metal hydroxides (e.g. sodium hydroxide, potassium hydroxide, etc.), and alkaline earth metal hydroxides (e.g. Magnesium hydroxide, calcium hydroxide, etc.). Among these, preferably one or more selected from carbonates and hydrogen carbonates (excluding potassium carbonate).
Examples of the salt include alkali metal salts such as sodium salts and potassium salts, alkaline earth metal salts such as magnesium and calcium salts, and ammonium salts.
（Ｆ）成分として、より具体的には、炭酸ナトリウム、炭酸アンモニウム、炭酸水素ナトリウム、炭酸水素カリウム、炭酸水素アンモニウム、水酸化ナトリウム、水酸化カリウム等が挙げられ、このうち、好ましくは炭酸ナトリウム、炭酸水素ナトリウム、炭酸水素カリウムから選ばれる１種以上である。 More specifically, component (F) includes sodium carbonate, ammonium carbonate, sodium hydrogen carbonate, potassium hydrogen carbonate, ammonium hydrogen carbonate, sodium hydroxide, potassium hydroxide, etc. Among these, sodium carbonate, One or more types selected from sodium hydrogen carbonate and potassium hydrogen carbonate.
本発明の消毒剤組成物は、（Ｆ）成分を、十分なウイルス不活化及び／又は殺菌等の消毒効果を得る観点から、好ましくは０．１質量％以上、より好ましくは０．２質量％以上、そして、配合成分の溶解性及び保存安定性の観点から、好ましくは０．５質量％以下、より好ましくは０．４質量％以下含有することができる。 In the disinfectant composition of the present invention, the component (F) is preferably 0.1% by mass or more, more preferably 0.2% by mass, from the viewpoint of obtaining disinfectant effects such as sufficient virus inactivation and/or sterilization. From the above, and from the viewpoint of solubility and storage stability of the compounded components, the content is preferably 0.5% by mass or less, more preferably 0.4% by mass or less.
本発明の消毒剤組成物は、本発明の効果を損なわない範囲で、任意にウイルス不活化剤を含むことができる。本発明の消毒剤組成物が、ウイルス不活化剤を含有することで、該消毒剤組成物のウイルス不活化効果がより向上する。
ウイルス不活化剤としては、例えば、緑茶抽出物、マキベリー抽出物、ブドウ抽出物、月見草種子抽出物、ウーロン茶抽出物、ビルベリー抽出物が挙げられる。
The disinfectant composition of the present invention may optionally contain a virus inactivating agent within a range that does not impair the effects of the present invention. When the disinfectant composition of the present invention contains a virus inactivating agent, the virus inactivating effect of the disinfectant composition is further improved.
Examples of virus inactivators include green tea extract, maqui berry extract, grape extract, evening primrose seed extract, oolong tea extract, and bilberry extract.
本発明の消毒剤組成物は、任意に、溶剤、界面活性剤、金属腐食抑制剤、キレート剤、緩衝剤、ポリリジン等の殺菌剤、酸化防止剤、防腐剤、香料、色素を含有することができる〔ただし、（Ａ）～（Ｆ）成分に相当するものを除く〕。 The disinfectant composition of the present invention may optionally contain a solvent, a surfactant, a metal corrosion inhibitor, a chelating agent, a buffer, a disinfectant such as polylysine, an antioxidant, a preservative, a fragrance, and a pigment. Yes (excluding those corresponding to components (A) to (F)).
本発明の消毒剤組成物は、十分なウイルス不活化及び／又は殺菌等の消毒効果を得る観点から、２５℃におけるｐＨが、好ましくは９．５以上、より好ましくは１０．０以上、更に好ましくは１０．５以上、そして、対象表面の保護及び皮膚マイルド性の観点から、好ましくは１３．０以下、より好ましくは１２．０以下、更に好ましくは１１．０以下である。本発明の消毒剤組成物のｐＨは、ＪＩＳ Ｚ－８８０２：２０１１「ｐＨ測定方法」にしたがって測定されたものであり、具体的には、以下の測定法にしたがって測定されたものである。 The disinfectant composition of the present invention has a pH at 25° C. of preferably 9.5 or higher, more preferably 10.0 or higher, and even more preferably is 10.5 or more, and preferably 13.0 or less, more preferably 12.0 or less, still more preferably 11.0 or less from the viewpoint of protecting the target surface and skin mildness. The pH of the disinfectant composition of the present invention was measured according to JIS Z-8802:2011 "pH measurement method", and specifically, according to the following measurement method.
＜ｐＨの測定法＞
ｐＨメーター（株式会社堀場製作所製、ｐＨ／イオンメーター Ｆ－７１）にｐＨ電極内部液を飽和塩化カリウム水溶液（３．３３モル／Ｌ）としたｐＨ測定用複合電極（株式会社堀場製作所製、ガラス摺り合わせスリーブ型）を接続する。次に、ｐＨ４．０１標準液（フタル酸塩標準液）、ｐＨ６．８６（中性リン酸塩標準液）、ｐＨ９．１８標準液（ホウ酸塩標準液）で校正操作を行う。測定対象となる消毒剤組成物をサンプル瓶に入れて予め２５℃の恒温槽に３０分間浸漬しておく。恒温に調整されたサンプルに前記のｐＨメーターの電極を浸漬し、１分後のｐＨを測定する。
<pH measurement method>
A composite electrode for pH measurement (manufactured by Horiba, Ltd., glass Connect the sliding sleeve type). Next, a calibration operation is performed using a pH 4.01 standard solution (phthalate standard solution), a pH 6.86 (neutral phosphate standard solution), and a pH 9.18 standard solution (borate standard solution). A disinfectant composition to be measured is placed in a sample bottle and immersed in a thermostatic bath at 25° C. for 30 minutes. The electrode of the pH meter is immersed in the sample whose temperature has been adjusted to a constant temperature, and the pH is measured after 1 minute.
本発明の消毒剤組成物は、配合成分の溶解性の観点から、水を含有する。水は、特に限定するものではないが、水道水、井戸水、イオン交換水、蒸留水等が挙げられる。水は、組成物の残部の量（合計が１００質量％となる量）で用いられることが好ましい。本発明の消毒剤組成物は、水を、例えば、１９質量％以上、更に２２質量％以上、更に２５質量％以上、そして、３９質量％以下、更に３７質量％以下、更に３５質量％以下含有することができる。 The disinfectant composition of the present invention contains water from the viewpoint of solubility of the ingredients. Examples of water include, but are not limited to, tap water, well water, ion exchange water, distilled water, and the like. Water is preferably used in an amount that accounts for the balance of the composition (an amount that adds up to 100% by weight). The disinfectant composition of the present invention contains water, for example, 19% by mass or more, further 22% by mass or more, further 25% by mass or more, and 39% by mass or less, further 37% by mass or less, further 35% by mass or less. can do.
本発明の消毒剤組成物が消毒する対象は、特に限定されないが、（１）硬質表面、例えば、調理器具、調理台、ショーケース、冷蔵庫、テーブル、机、椅子、ドアノブ、トイレ（便器、便座）、水道の蛇口、食器、食品加工設備、壁、床等、（２）食品、例えば、野菜等の食品素材表面、（３）身体、例えば人の皮膚、手指等、が挙げられる。
本発明の消毒剤組成物は、特に、飲食店の厨房、調理器具、ショーケース、調理台、冷蔵庫、机、椅子等、食品加工工場の、食品容器、調理台、机、椅子、収納箱等、の硬質表面の消毒に好適に用いることができる。
The objects to be disinfected by the disinfectant composition of the present invention are not particularly limited. ), water faucets, tableware, food processing equipment, walls, floors, etc., (2) food, for example, the surface of food materials such as vegetables, (3) the body, for example, human skin, fingers, etc.
The disinfectant composition of the present invention is particularly suitable for kitchens, cooking utensils, showcases, countertops, refrigerators, desks, chairs, etc. of restaurants, food containers, countertops, desks, chairs, storage boxes, etc. of food processing factories, etc. It can be suitably used for disinfecting hard surfaces of .
本発明の消毒剤組成物は、アルコール系消毒剤として公知の使用方法を用いることができる。例えば、本発明の消毒剤組成物をスプレー等により消毒される対象表面に噴霧し、不織布等で拭き取りを行う方法や、該消毒剤組成物を含浸させた不織布等を用いて消毒される対象表面を拭き上げる方法等が挙げられる。 The disinfectant composition of the present invention can be used in a manner known as an alcohol-based disinfectant. For example, a method of spraying the disinfectant composition of the present invention onto the surface to be disinfected by spraying or the like and wiping it off with a non-woven cloth, or a method of spraying the disinfectant composition of the present invention onto the surface to be disinfected using a non-woven cloth etc. impregnated with the disinfectant composition; Examples include a method of wiping it off.
本発明の消毒剤組成物は、対象表面のウイルスの不活化、除ウイルス、抗ウイルス、細菌若しくは真菌等の微生物の殺菌、除菌並びに抗菌に好適に用いることができる。本発明の消毒剤組成物は、ウイルスや食中毒菌等を消毒するために用いることができる。 The disinfectant composition of the present invention can be suitably used for virus inactivation, virus removal, antivirus, sterilization of microorganisms such as bacteria or fungi, sterilization, and antibacterial purposes on the surface of an object. The disinfectant composition of the present invention can be used to disinfect viruses, food poisoning bacteria, and the like.
本発明の消毒剤組成物の対象とするウイルスとしては、非エンベロープウイルスやエンベロープウイルスが挙げられ、特に非エンベロープウイルス、更にはノロウイルス属に属するウイルスが挙げられる。
非エンベロープウイルスは、例えば、ウイルス表面に脂質層若しくは脂質２重層を持たない、一本鎖（＋）ＲＮＡウイルス、一本鎖（－）ＲＮＡウイルス、二本鎖ＲＮＡウイルス、一本鎖ＤＮＡウイルス、及び二本鎖ＤＮＡウイルスであってよい。
非エンベロープウイルスとしては、例えば、ＤＮＡをゲノムとするウイルスについては、アデノウイルス、パルボウイルス、パポバウイルス、ヒトパピローマウイルス等、ＲＮＡをゲノムとするウイルスについては、ロタウイルス、コクサッキーウイルス、エンテロウイルス、サポウイルス、ノロウイルス、ポリオウイルス、エコーウイルス、Ａ型肝炎ウイルス、Ｅ型肝炎ウイルス、ライノウイルス、アストロウイルス、バクテリオファージＭＳ２等が挙げられる。
また、エンベロープウイルスとは、ウイルス表面に脂質層もしくは脂質２重層を持つ、一本鎖（＋）ＲＮＡウイルス、一本鎖（－）ＲＮＡウイルス、二本鎖ＲＮＡウイルス、一本鎖ＤＮＡウイルス、及び二本鎖ＤＮＡウイルスであってよい。
エンベロープウイルスとしては、ヘルペスウイルス、インフルエンザウイルス、呼吸器合胞体ウイルス、コロナウイルス、ＨＩＶ、Ｂ型肝炎ウイルス、Ｃ型肝炎ウイルス、Ｄ型肝炎ウイルス、風疹ウイルス等が挙げられる。
本発明の消毒剤組成物は、エンベロープウイルスだけでなく、非エンベロープウイルス、特に、ノロウイルス属に属するウイルスに対しても、高いウイルス不活化効果を有する。
Viruses targeted by the disinfectant composition of the present invention include non-enveloped viruses and enveloped viruses, particularly non-enveloped viruses, and further include viruses belonging to the genus Norovirus.
Non-enveloped viruses include, for example, single-stranded (+) RNA viruses, single-stranded (-) RNA viruses, double-stranded RNA viruses, single-stranded DNA viruses, which do not have a lipid layer or lipid bilayer on the virus surface. and double-stranded DNA viruses.
Non-enveloped viruses include, for example, viruses whose genome is DNA such as adenovirus, parvovirus, papovavirus, human papillomavirus, etc. Viruses whose genome is RNA include rotavirus, coxsackievirus, enterovirus, sapovirus, norovirus, etc. Examples include poliovirus, echovirus, hepatitis A virus, hepatitis E virus, rhinovirus, astrovirus, and bacteriophage MS2.
In addition, enveloped viruses include single-stranded (+) RNA viruses, single-stranded (-) RNA viruses, double-stranded RNA viruses, single-stranded DNA viruses, and It may be a double-stranded DNA virus.
Examples of enveloped viruses include herpes virus, influenza virus, respiratory syncytial virus, coronavirus, HIV, hepatitis B virus, hepatitis C virus, hepatitis D virus, rubella virus, and the like.
The disinfectant composition of the present invention has a high virus inactivation effect not only on enveloped viruses but also on non-enveloped viruses, especially viruses belonging to the genus Norovirus.
また、本発明の消毒剤組成物の対象とする食材を汚染する細菌、真菌としては、カンピロバクター、大腸菌、サルモネラ菌、腸炎ビブリオ、赤痢菌、緑膿菌、アスペルギルス・フラバス等が挙げられる。 Bacteria and fungi that contaminate foodstuffs targeted by the disinfectant composition of the present invention include Campylobacter, Escherichia coli, Salmonella enterica, Vibrio parahaemolyticus, Shigella, Pseudomonas aeruginosa, Aspergillus flavus, and the like.
本発明の消毒剤組成物は、（Ａ）エタノールを６０質量％以上８０質量％以下、（Ｂ）炭酸カリウムを０．３０質量％以上０．８０質量％以下、（Ｃ）コハク酸、リンゴ酸、フマル酸、アジピン酸及びそれらの塩から選ばれる１種以上を０．０６質量％以上０．３０質量％以下、並びに（Ｄ）クエン酸及び／又はその塩を０．０２質量％以上０．１０質量％以下配合してなり、ｐＨが２５℃で９．５以上である、消毒剤組成物であってよい。 The disinfectant composition of the present invention includes (A) 60% by mass or more and 80% by mass or less of ethanol, (B) 0.30% by mass or more and 0.80% by mass or less of potassium carbonate, (C) succinic acid, and malic acid. , 0.06% by mass or more and 0.30% by mass or less of one or more selected from fumaric acid, adipic acid, and their salts, and (D) 0.02% by mass or more of citric acid and/or its salt. The disinfectant composition may contain 10% by mass or less and have a pH of 9.5 or more at 25°C.
また、本発明の消毒剤組成物は、（Ａ）エタノールを６０質量％以上８０質量％以下、（Ｂ）炭酸カリウムを０．３０質量％以上０．８０質量％以下、（Ｃ）コハク酸、リンゴ酸、フマル酸、アジピン酸から選ばれる１種以上を０．０６質量％以上０．３０質量％以下、及び（Ｄ）クエン酸を０．０２質量％以上０．１０質量％以下配合してなり、ｐＨが２５℃で９．５以上である、消毒剤組成物であってよい。 Further, the disinfectant composition of the present invention includes (A) ethanol of 60% by mass or more and 80% by mass or less, (B) potassium carbonate of 0.30% by mass or more and 0.80% by mass or less, (C) succinic acid, One or more selected from malic acid, fumaric acid, and adipic acid from 0.06% by mass to 0.30% by mass, and (D) citric acid from 0.02% by mass to 0.10% by mass. The disinfectant composition may have a pH of 9.5 or higher at 25°C.
＜ウイルス不活化方法、殺菌方法＞
本発明は、本発明の消毒剤組成物を、対象表面、特にウイルスが存在する対象表面に接触させるウイルス不活化方法を提供する。また、本発明は、本発明の消毒剤組成物を対象表面、特に微生物（例えば、細菌や真菌等）が存在する対象表面に接触させる殺菌方法を提供する。本発明のウイルス不活化方法は、本発明の消毒剤組成物を、ウイルスが存在する対象表面に接触させる、ウイルスの不活化方法であってよい。また、本発明の殺菌方法は、本発明の消毒剤組成物を、微生物が存在する対象表面に接触させる、微生物の殺菌方法であってよい。
本発明のウイルス不活化方法は、対象表面において、ウイルスの不活化と、微生物の殺菌を行うものであってよい。また、本発明の殺菌方法は、対象表面において、微生物の殺菌と、ウイルスの不活化を行うものであってよい。
本発明のウイルス不活化方法及び殺菌方法では、本発明の消毒剤組成物を薄めずに、対象表面に接触させることができる。
本発明のウイルス不活化方法及び殺菌方法に用いる消毒剤組成物は、本発明の消毒剤組成物で記載した態様を適宜適用することができる。
<Virus inactivation method, sterilization method>
The present invention provides a virus inactivation method in which the disinfectant composition of the present invention is brought into contact with a target surface, particularly a target surface where a virus is present. The present invention also provides a disinfection method in which the disinfectant composition of the present invention is brought into contact with a target surface, particularly a target surface where microorganisms (eg, bacteria, fungi, etc.) are present. The virus inactivation method of the present invention may be a method of inactivating a virus, in which the disinfectant composition of the present invention is brought into contact with a target surface where a virus is present. Moreover, the sterilization method of the present invention may be a method of sterilizing microorganisms, in which the disinfectant composition of the present invention is brought into contact with a target surface where microorganisms are present.
The virus inactivation method of the present invention may inactivate viruses and sterilize microorganisms on the target surface. Furthermore, the sterilization method of the present invention may sterilize microorganisms and inactivate viruses on the target surface.
In the virus inactivation method and sterilization method of the present invention, the disinfectant composition of the present invention can be brought into contact with the target surface without being diluted.
For the disinfectant composition used in the virus inactivation method and sterilization method of the present invention, the embodiments described for the disinfectant composition of the present invention can be applied as appropriate.
本発明のウイルス不活化方法は、本発明の消毒剤組成物を、硬質表面、特にウイルスが存在する硬質表面に接触させる、ウイルス不活化方法であってよい。
また、本発明の殺菌方法は、本発明の消毒剤組成物を、硬質表面、特に微生物が存在する硬質表面に接触させる、殺菌方法であってよい。
The virus inactivation method of the present invention may be a virus inactivation method in which the disinfectant composition of the present invention is brought into contact with a hard surface, particularly a hard surface where a virus is present.
Furthermore, the sterilization method of the present invention may be a sterilization method in which the disinfectant composition of the present invention is brought into contact with a hard surface, particularly a hard surface where microorganisms are present.
本発明のウイルス不活化方法及び殺菌方法の対象表面は、特に限定されないが、（１）硬質表面、例えば、調理器具、調理台、ショーケース、冷蔵庫、テーブル、机、椅子、ドアノブ、トイレ（便器、便座）、水道の蛇口、食器、食品加工設備、壁、床等、（２）食品、例えば、野菜等の食品素材表面、（３）身体、例えば人の皮膚、手指等、が挙げられる。
本発明のウイルス不活化方法及び殺菌方法の対象表面は、特に、飲食店の厨房、調理器具、ショーケース、調理台、冷蔵庫、机、椅子等、食品加工工場の、食品容器、調理台、机、椅子、収納箱等、の硬質表面が好ましい。
The target surfaces of the virus inactivation method and sterilization method of the present invention are not particularly limited, but include (1) hard surfaces, such as cooking utensils, countertops, showcases, refrigerators, tables, desks, chairs, doorknobs, and toilets (toilet bowls); , toilet seats), water faucets, tableware, food processing equipment, walls, floors, etc., (2) food, for example, the surface of food materials such as vegetables, and (3) the body, for example, human skin, fingers, etc.
Target surfaces of the virus inactivation method and sterilization method of the present invention are, in particular, kitchens, cooking utensils, showcases, countertops, refrigerators, desks, and chairs of restaurants, food containers, countertops, and desks of food processing factories. Hard surfaces such as , chairs, storage boxes, etc. are preferred.
本発明の消毒剤組成物をウイルス及び／又は細菌、真菌等の微生物が存在する対象表面に接触させる方法としては、ウイルス及び／又は細菌、真菌等の微生物が存在する、又は存在すると考えられる対象表面に、本発明の消毒剤組成物を噴霧又は塗布する方法や、本発明の消毒剤組成物に対象物品を浸漬させる方法が挙げられる。また、本発明の消毒剤組成物を不織布等の基材に含浸させて、本発明の消毒剤組成物が含浸されている基材を対象表面に接触させてもよい。 A method for bringing the disinfectant composition of the present invention into contact with a target surface on which viruses and/or microorganisms such as bacteria and fungi exist is an object on which viruses and/or microorganisms such as bacteria and fungi exist or are thought to exist. Examples include a method of spraying or applying the disinfectant composition of the present invention onto a surface, and a method of immersing the target article in the disinfectant composition of the present invention. Alternatively, a base material such as a nonwoven fabric may be impregnated with the disinfectant composition of the present invention, and the base material impregnated with the disinfectant composition of the present invention may be brought into contact with the target surface.
対象表面に、本発明の消毒剤組成物を噴霧又は塗布する場合、本発明の消毒剤組成物を、スプレイヤーを具備する容器に充填して、液滴状又は泡状にして噴霧したり、容器から本発明の消毒剤組成物を対象表面に流して、はけ等により塗布したりすればよい。
スプレイヤーを具備する容器は、トリガー式スプレー容器、ポンプ式スプレー容器等の噴射剤を使用しない手動式スプレー装置、噴射剤を用いるエアゾール等が挙げられる。
前記スプレイヤーを具備する容器は、内容物を液滴状又は泡状にして噴霧することができるトリガー式スプレーが好ましく、内容物を液滴状に噴霧する機構を備えたトリガー式スプレー又は泡を形成する機構（泡形成機構）を備えたトリガー式スプレーがより好ましい。
When spraying or applying the disinfectant composition of the present invention to the target surface, the disinfectant composition of the present invention is filled in a container equipped with a sprayer and sprayed in the form of droplets or foam, The disinfectant composition of the present invention may be poured from a container onto the target surface and applied with a brush or the like.
Containers equipped with a sprayer include manual spray devices that do not use propellants, such as trigger-type spray containers and pump-type spray containers, and aerosols that use propellants.
The container equipped with the sprayer is preferably a trigger-type sprayer that can spray the contents in the form of droplets or foam, and a trigger-type sprayer or foamer equipped with a mechanism for spraying the contents in the form of droplets or foam. A trigger-type spray equipped with a foam-forming mechanism (foam-forming mechanism) is more preferred.
本発明の消毒剤組成物を不織布に含浸させて、対象表面に接触させる場合、不織布は、シート状に加工したものを用いることができ、不織布を構成する繊維は、親水性繊維及び疎水性繊維から選ばれる１種以上の繊維から構成されるものが好ましい。
本発明において、親水性繊維とは、標準状態の水分率（２０℃、６５％ＲＨ）が５質量％を超える繊維を指している。なお標準状態の水分率は、ＪＩＳ Ｌ １０１３、ＪＩＳ Ｌ １０１５に規定される方法により測定される。また疎水性繊維とは、標準状態の水分率（２０℃、６５％ＲＨ）が５質量％以下の繊維を指す。
本発明の消毒剤組成物を対象表面に接触させる方法は、対象表面に本発明の消毒剤組成物を含浸させた不織布を押し当てて、対象物に損傷を与えない範囲で外力を加えて、不織布に含浸した本発明の消毒剤組成物を対象表面に移して接触させればよく、擦る、拭くのいずれであってもよい。
When a nonwoven fabric is impregnated with the disinfectant composition of the present invention and brought into contact with the target surface, the nonwoven fabric can be processed into a sheet, and the fibers constituting the nonwoven fabric include hydrophilic fibers and hydrophobic fibers. It is preferable to use one or more fibers selected from the following.
In the present invention, hydrophilic fibers refer to fibers with a standard moisture content (20° C., 65% RH) of more than 5% by mass. Note that the moisture content in the standard state is measured by the method specified in JIS L 1013 and JIS L 1015. Furthermore, the term "hydrophobic fiber" refers to a fiber with a standard moisture content (20° C., 65% RH) of 5% by mass or less.
The method of bringing the disinfectant composition of the present invention into contact with a target surface is to press a nonwoven fabric impregnated with the disinfectant composition of the present invention against the target surface, apply external force within a range that does not damage the target object, The disinfectant composition of the present invention impregnated into a nonwoven fabric may be transferred to and brought into contact with the target surface, and the disinfectant composition may be rubbed or wiped.
本発明の消毒剤組成物の使用量は特に限定されるものではないが、例えば、対象物の単位面積１ｃｍ２あたり、（ａ）成分の量として、ウイルス不活化性能及び使用後の仕上がり性の観点から、好ましくは０．００１ｇ以上、より好ましくは０．０１ｇ以上、そして、好ましくは１ｇ以下、より好ましくは０．１ｇ以下とすることができる。 Although the amount of the disinfectant composition of the present invention to be used is not particularly limited, for example, the amount of component (a) per unit area of 1 cm 2 of the object may be determined based on virus inactivation performance and finish quality after use. From this point of view, the amount can be preferably 0.001 g or more, more preferably 0.01 g or more, and preferably 1 g or less, more preferably 0.1 g or less.
本発明の消毒剤組成物を、ウイルス及び／又は細菌、真菌等の微生物が存在する対象表面に接触させる時間（放置させる時間）は、ウイルス不活化性能及び／又は殺菌性能の観点から、好ましくは３０秒以上である。
接触させた後は、そのまま乾燥させてもよいし、綺麗な布等で拭き取ってもよいし、水ですすいでもよい。すすぐ際は、スポンジ等で外力（物理的力）をかけてもよく、単に水流ですすいでもよい。
The time for which the disinfectant composition of the present invention is brought into contact with the target surface on which viruses and/or microorganisms such as bacteria and fungi are present (the time for which it is left to stand) is preferably from the viewpoint of virus inactivation performance and/or sterilization performance. It is 30 seconds or more.
After contact, it may be left to dry, wiped off with a clean cloth, or rinsed with water. When rinsing, external force (physical force) may be applied using a sponge or the like, or it may be simply rinsed with water.
＜消毒剤組成物含浸物品＞
本発明の消毒剤組成物は、基体に含浸させて消毒剤組成物含浸物品として使用することもできる。本発明の消毒剤組成物を基体に含浸させた消毒剤組成物含浸物品は、消毒作業の作業性の観点から好ましい。
すなわち、本発明は、本発明の消毒剤組成物が基体に含浸されている、消毒剤組成物含浸物品を提供する。本発明の消毒剤組成物含浸物品は、硬質表面用消毒剤組成物含浸物品であってよい。
<Article impregnated with disinfectant composition>
The disinfectant composition of the present invention can also be used as a disinfectant composition-impregnated article by impregnating a substrate. A disinfectant composition-impregnated article in which a substrate is impregnated with the disinfectant composition of the present invention is preferable from the viewpoint of workability in disinfection work.
That is, the present invention provides disinfectant composition-impregnated articles in which a substrate is impregnated with the disinfectant composition of the present invention. The disinfectant composition-impregnated article of the present invention may be a hard surface disinfectant composition-impregnated article.
本発明の消毒剤組成物含浸物品に用いられる基体としては、柔軟性、屈曲性又は可撓性を有し、消毒剤組成物が含浸可能なものであり、使用時に十分な強度を有し、くず等の発生の無いものが用いられる。無荷重下において後述の量の消毒剤組成物を含浸しうる基体を用いることが好ましい。 The substrate used in the disinfectant composition-impregnated article of the present invention has flexibility, bendability, or flexibility, can be impregnated with the disinfectant composition, and has sufficient strength during use; A material that does not generate waste etc. is used. It is preferred to use a substrate that can be impregnated with the disinfectant composition in the amount described below under no load.
そのような基体としては、繊維状材料から構成される繊維構造体、例えば、各種紙、不織布、織布若しくは編布が挙げられる。これらの繊維構造体を構成する繊維状材料としては、例えば、セルロース系繊維、変性セルロース系繊維、合成繊維及びこれらの２種以上の混合物等が挙げられる。
また、樹脂中に気泡を分散させて得られる多孔質構造体（例えば、スポンジ状構造体）も上記基体として使用できる。これら基体は、基体シートであってよい。
Such substrates include fibrous structures composed of fibrous materials, such as various papers, non-woven fabrics, woven fabrics or knitted fabrics. Examples of the fibrous materials constituting these fibrous structures include cellulose fibers, modified cellulose fibers, synthetic fibers, and mixtures of two or more thereof.
Further, a porous structure (for example, a sponge-like structure) obtained by dispersing air bubbles in a resin can also be used as the substrate. These substrates may be substrate sheets.
本発明の消毒剤組成物を含浸させた消毒剤組成物含浸物品を用いて硬質表面を消毒する方法において、本発明の消毒剤組成物を含浸させた消毒剤組成物含浸物品は、基体に予め消毒剤組成物を含浸させてなるもの、あるいは乾燥した基体に消毒剤組成物を使用直前に含浸させてなるもの、の何れでもよい。また消毒剤組成物を含浸させた消毒剤組成物含浸物品は、モップ状の掃除具に装着されて用いられてもよいし、直接手で持って拭き掃除に用いられてもよい。 In the method of disinfecting a hard surface using a disinfectant composition-impregnated article impregnated with the disinfectant composition of the present invention, the disinfectant composition-impregnated article impregnated with the disinfectant composition of the present invention is applied to a substrate in advance. The substrate may be impregnated with a disinfectant composition, or a dried substrate may be impregnated with a disinfectant composition immediately before use. Further, the disinfectant composition-impregnated article impregnated with the disinfectant composition may be used by being attached to a mop-like cleaning tool, or may be directly held in the hand and used for wiping.
予め消毒剤組成物が基体に含浸されている場合、消毒剤組成物の含浸率は消毒効果の観点から、基体質量〔即ち、未含浸状態（乾燥状態）の基体の質量基準〕あたり、１００質量％以上であることが好ましく、１５０質量％以上であることがより好ましく、そして、１，０００質量％以下であることが好ましく、５００質量％以下であることがより好ましく、３５０質量％以下であることが更に好ましい。含浸率が１００質量％以上であれば、十分な消毒効果が得られる。また、含浸率が１，０００質量％以下であれば、消毒面以外の硬質表面に消毒剤組成物を接触させることなく使用できる。消毒効果の一層の向上の観点から、基体が基体シートである場合、消毒剤組成物の含浸前の坪量は、一定面積の消毒に必要な消毒剤組成物の保持性とシートの操作性やコストとの観点から、例えば、５０ｇ／ｍ２以上であることが好ましく、１００ｇ／ｍ２以上であることがより好ましく、そして、２５０ｇ／ｍ２以下であることが好ましく、１５０ｇ／ｍ２以下であることがより好ましい。 When the substrate is impregnated with the disinfectant composition in advance, the impregnation rate of the disinfectant composition is 100 mass per substrate mass [i.e., based on the mass of the unimpregnated (dry state) substrate] from the viewpoint of disinfection effect. % or more, more preferably 150% by mass or more, and preferably 1,000% by mass or less, more preferably 500% by mass or less, and 350% by mass or less. More preferably. If the impregnation rate is 100% by mass or more, a sufficient disinfecting effect can be obtained. Further, if the impregnation rate is 1,000% by mass or less, the disinfectant composition can be used without contacting hard surfaces other than the disinfecting surface. From the perspective of further improving the disinfection effect, when the substrate is a base sheet, the basis weight before impregnation with the disinfectant composition should be determined based on the ability to retain the disinfectant composition necessary for disinfecting a certain area, the operability of the sheet, and the basis weight before impregnation with the disinfectant composition. From the viewpoint of cost, for example, it is preferably 50 g/m 2 or more, more preferably 100 g/m 2 or more, and preferably 250 g/m 2 or less, and 150 g/m 2 or less. It is more preferable that there be.
本発明の消毒剤組成物を含浸させた消毒剤組成物含浸物品の使用時に、別途本発明の消毒剤組成物を、被消毒対象表面又は消毒剤組成物含浸物品に、噴霧しながら使用してもよい。当該使用方法によって、より広い面積を消毒することができる。 When using a disinfectant composition-impregnated article impregnated with the disinfectant composition of the present invention, the disinfectant composition of the present invention is separately used while spraying the surface to be disinfected or the disinfectant composition-impregnated article. Good too. With this method of use, a larger area can be disinfected.
＜消毒剤組成物の製造方法＞
本発明は、（Ａ）エタノール〔以下、（Ａ）成分という〕、（Ｂ）炭酸カリウム〔以下、（Ｂ）成分という〕、（Ｃ）コハク酸、リンゴ酸、フマル酸、アジピン酸及びそれらの塩から選ばれる１種以上〔以下、（Ｃ）成分という〕、並びに（Ｄ）クエン酸及び／又はその塩〔以下、（Ｄ）成分という〕を混合し、
（Ａ）成分を６０質量％以上８０質量％以下、（Ｂ）成分を０．３０質量％以上０．８０質量％以下、（Ｃ）成分を０．０６質量％以上０．３０質量％以下、（Ｄ）成分を０．０２質量％以上０．１０質量％以下含有し、ｐＨが２５℃で９．５以上である消毒剤組成物を製造する、消毒剤組成物の製造方法を提供する。
本発明の消毒剤組成物の製造方法では、上記本発明の消毒剤組成物で述べた事項を適宜適用することができる。本発明の消毒剤組成物における各成分の好ましい含有量は、本発明の消毒剤組成物の製造方法における、好ましい配合量である。
<Method for producing disinfectant composition>
The present invention provides (A) ethanol [hereinafter referred to as component (A)], (B) potassium carbonate [hereinafter referred to as component (B)], (C) succinic acid, malic acid, fumaric acid, adipic acid, and their Mixing one or more selected from salts [hereinafter referred to as component (C)] and (D) citric acid and/or its salt [hereinafter referred to as component (D)],
(A) component is 60% by mass or more and 80% by mass or less, (B) component is 0.30% by mass or more and 0.80% by mass or less, (C) component is 0.06% by mass or more and 0.30% by mass or less, Provided is a method for producing a disinfectant composition that contains component (D) in an amount of 0.02% by mass or more and 0.10% by mass or less and has a pH of 9.5 or more at 25°C.
In the method for producing the disinfectant composition of the present invention, the matters described above for the disinfectant composition of the present invention can be applied as appropriate. The preferred content of each component in the disinfectant composition of the present invention is the preferred blending amount in the method for producing the disinfectant composition of the present invention.
［配合成分］
表１の消毒剤組成物の調製には、下記の成分を用いた。
＜（Ａ）成分＞
・エタノール（富士フイルム和光純薬株式会社製）
＜（Ｂ）成分＞
・炭酸カリウム（ＡＧＣ株式会社製）
＜（Ｃ）成分＞
・コハク酸（株式会社日本触媒製）
・リンゴ酸（扶桑化学工業株式会社製）
・フマル酸（扶桑化学工業株式会社製）
・アジピン酸（昭和化工株式会社製）
＜（Ｄ）成分＞
・クエン酸（扶桑化学工業株式会社製）
＜（Ｅ）成分＞
・酒石酸（扶桑化学工業株式会社製）
＜（Ｆ）成分＞
・炭酸水素カリウム（富士フイルム和光純薬株式会社製）
・炭酸水素ナトリウム（富士フイルム和光純薬株式会社製）
・水酸化カリウム（富士フイルム和光純薬株式会社製）
[Ingredients]
The following ingredients were used to prepare the disinfectant compositions in Table 1.
<(A) component>
・Ethanol (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.)
<(B) component>
・Potassium carbonate (manufactured by AGC Corporation)
<(C) component>
・Succinic acid (manufactured by Nippon Shokubai Co., Ltd.)
・Malic acid (manufactured by Fuso Chemical Industry Co., Ltd.)
・Fumaric acid (manufactured by Fuso Chemical Industry Co., Ltd.)
・Adipic acid (manufactured by Showa Kako Co., Ltd.)
<(D) component>
・Citric acid (manufactured by Fuso Chemical Industry Co., Ltd.)
<(E) component>
・Tartaric acid (manufactured by Fuso Chemical Industry Co., Ltd.)
<(F) component>
・Potassium hydrogen carbonate (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.)
・Sodium hydrogen carbonate (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.)
・Potassium hydroxide (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.)
［消毒剤組成物の調製方法］
表１に示す組成に従い、各成分を２５℃で混合し、実施例１－１～１－９及び比較例１－１～１－１２の消毒剤組成物８００ｇを得た。
[Method for preparing disinfectant composition]
According to the composition shown in Table 1, each component was mixed at 25° C. to obtain 800 g of disinfectant compositions of Examples 1-1 to 1-9 and Comparative Examples 1-1 to 1-12.
［溶解性の評価方法］
表１に示す消毒剤組成物の調製直後の外観を目視により観察し、下記基準に従って、各消毒剤組成物の溶解性を評価した。溶解性の評価が×だった組成物に関しては、白残り等の評価は行わなかった。
○：良い（均一で透明な液である）
×：悪い（液の分離や成分の析出が認められる）
[Solubility evaluation method]
The appearance of the disinfectant compositions shown in Table 1 immediately after preparation was visually observed, and the solubility of each disinfectant composition was evaluated according to the following criteria. For compositions with a solubility evaluation of ×, no evaluation of white residue, etc. was performed.
○: Good (uniform and transparent liquid)
×: Bad (separation of liquid and precipitation of components are observed)
［白残りの評価方法］
表１に記載の各消毒剤組成物をスプレイヤーに入れ、それを用いて黒色のポリプロピレン製テストピース（７ｃｍ×１５ｃｍ）に向けてそれぞれ２ｍＬ噴霧して、室温（２５℃）で放置した。２時間後、その表面を目視で観察して、下記基準に従って、各消毒剤組成物の白残りを評価した。
Ａ：白く残らない
Ｂ：ほとんど白く残らない
Ｃ：やや白く残る
Ｄ：白く残る
[How to evaluate white residue]
Each disinfectant composition listed in Table 1 was placed in a sprayer, and 2 mL of each was sprayed onto a black polypropylene test piece (7 cm x 15 cm) and left at room temperature (25°C). After 2 hours, the surface was visually observed and the white residue of each disinfectant composition was evaluated according to the following criteria.
A: Does not remain white B: Almost does not remain white C: Remains slightly white D: Remains white
［ネコカリシウイルス不活化効果の評価方法］
ウイルス不活化試験はウイルスの不活化評価の標準法として一般的に知られている「平成２７年度ノロウイルスの不活化条件に関する調査報告書」（国立医薬品食品衛生研究所）及びＪＩＳ Ｌ １９２２を参考に行った。
実施例１－１～１－９及び比較例１－１～１－１２の各消毒剤組成物９０μＬと１．０×１０８ＰＦＵ／ｍＬに調整したネコカリシウイルス液（Ｆ－９株、ＡＴＣＣ ＶＲ－７８２）１０μＬをエッペンチューブに入れ、よく混合した。３０秒経過後に、クエンチ液（Ｄ／Ｅ Ｎｅｕｔｒａｌｉｚｉｎｇ Ｂｒｏｔｈ）で１／１０希釈して反応を停止した。なお、ブランクとして、消毒剤組成物９０μＬの代わりにＰＢＳ（リン酸緩衝生理食塩水）９０μＬを用い同様の操作を行った。
反応停止後、混合物をＤＭＥＭ培地（ダルベッコ改変イーグル培地）で更に１／１０希釈し、その０．５ｍＬを、ＰＢＳ（リン酸緩衝生理食塩水）で洗ったＣＲＦＫ細胞（ネコ腎由来株化細胞）（ＡＴＣＣ ＣＣＬ－９４）に感染させ、１時間後にメチルセルロース含有培地に置換した。２日後に、それぞれの所定時間のサンプルをクリスタルバイオレットで細胞を染色し、プラーク数をカウントして、ブランクのプラーク数との対数の差を対数減少値として求め、以下の基準に従って評価した。
Ａ：対数減少値が４以上
Ｂ：対数減少値が３以上４未満
Ｃ：対数減少値が２以上３未満
Ｄ：対数減少値が２未満
[Method for evaluating feline calicivirus inactivation effect]
The virus inactivation test was conducted based on the "2015 Survey Report on Inactivation Conditions for Norovirus" (National Institute of Health Sciences) and JIS L 1922, which are generally known as standard methods for evaluating virus inactivation. went.
90 μL of each disinfectant composition of Examples 1-1 to 1-9 and Comparative Examples 1-1 to 1-12 and a feline calicivirus solution (F-9 strain, ATCC VR-782) was placed in an Eppendorf tube and mixed well. After 30 seconds, the reaction was stopped by diluting it 1/10 with a quenching liquid (D/E Neutralizing Broth). As a blank, the same operation was performed using 90 μL of PBS (phosphate buffered saline) instead of 90 μL of the disinfectant composition.
After stopping the reaction, the mixture was further diluted 1/10 with DMEM medium (Dulbecco's modified Eagle medium), and 0.5 mL of the mixture was added to CRFK cells (feline kidney-derived cell line) washed with PBS (phosphate buffered saline). (ATCC CCL-94), and after 1 hour, the medium was replaced with methylcellulose-containing medium. Two days later, cells were stained with crystal violet for each sample at a predetermined time, the number of plaques was counted, and the logarithmic difference from the blank number of plaques was determined as a logarithmic reduction value, and evaluated according to the following criteria.
A: Logarithmic reduction value is 4 or more B: Logarithmic reduction value is 3 or more and less than 4 C: Logarithmic reduction value is 2 or more and less than 3 D: Logarithmic reduction value is less than 2
表１に示すように、本発明に該当する実施例１－１～１－９の消毒剤組成物は、消毒される対象表面に白残りが生じずに、十分なネコカリシウイルス不活化効果を有するものであった。一方、炭酸カリウムを含まない比較例１－１、コハク酸を多く含む比較例１－５、比較例１－１１では、十分なネコカリシウイルス不活化効果が得られなかった。また、炭酸カリウムを多く含む比較例１－４、クエン酸を多く含む比較例１－６、及びコハク酸、リンゴ酸、フマル酸、アジピン酸のいずれも含まない比較例１－２では、配合成分の溶解が困難であった。コハク酸を多く含む比較例１－５、比較例１－１１、及びクエン酸を含まない比較例１－３、比較例１－７～１－１０、比較例１－１２では、テストピース表面に白残りが生じた。 As shown in Table 1, the disinfectant compositions of Examples 1-1 to 1-9, which correspond to the present invention, exhibited sufficient feline calicivirus inactivation effects without leaving any white residue on the surfaces to be disinfected. It was something that I had. On the other hand, in Comparative Example 1-1 which did not contain potassium carbonate, Comparative Example 1-5 and Comparative Example 1-11 which contained a large amount of succinic acid, a sufficient feline calicivirus inactivation effect was not obtained. In addition, in Comparative Example 1-4 containing a large amount of potassium carbonate, Comparative Example 1-6 containing a large amount of citric acid, and Comparative Example 1-2 containing neither succinic acid, malic acid, fumaric acid, nor adipic acid, the formulation components was difficult to dissolve. In Comparative Examples 1-5 and 1-11 that contain a large amount of succinic acid, and Comparative Examples 1-3, Comparative Examples 1-7 to 1-10, and Comparative Examples 1-12 that do not contain citric acid, the surface of the test piece A white residue was left.
［ヒトノロウイルス不活化効果の評価方法］
（１）ｈｕｍａｎ Ｓｍａｌｌ Ｉｎｔｅｓｔｉｎｅ Ｏｒｇａｎｏｉｄ（ｈＳＩＯ）の培養
ｈＳＩＯは２４ウェルプレート上でマトリゲル（Ｃｏｒｎｉｎｇ， ３５６２３１）に包埋し３Ｄ培養した。培地はＩｎｔｅｓｔｉＣｕｌｔ Ｏｒｇａｎｏｉｄ Ｇｒｏｗｔｈ Ｍｅｄｉｕｍ（Ｈｕｍａｎ）（ＳＴＥＭＣＥＬＬ Ｔｅｃｈｎｏｌｏｇｉｅｓ，ＳＴ－０６０１０）を用いた。培地交換・継代・９６ウェルプレートを用いた単層化の手技はユーザーマニュアルに従った。トリプシン処理後２日間はアノイキスを阻害するために培地に終濃度１０μＭとなるようにＲＯＣＫ（Ｒｈｏ－ａｓｓｏｃｉａｔｅｄ ｃｏｉｌｅｄ－ｃｏｉｌ ｆｏｒｍｉｎｇ ｋｉｎａｓｅ／Ｒｈｏ結合キナーゼ）阻害剤であるＣｕｌｔｕｒｅＳｕｒｅ Ｙ－２７６３２（富士フイルム和光純薬，０３６－２４０２３）を添加した。５００ｍＬのＡｄｖａｎｃｅｄ ＤＭＥＭ／Ｆ１２（Ｇｉｂｃｏ，１２６３４０１０）に５ｍＬのＧｌｕｔａＭＡＸ Ｉ（１００×）（Ｇｉｂｃｏ，３５０５０－０６１）、５ｍＬの１Ｍ ＨＥＰＥＳ（Ｇｉｂｃｏ，１５６３００８０）、５ｍＬのＰｅｎｉｃｉｌｌｉｎ－Ｓｔｒｅｐｔｏｍｙｃｉｎ（Ｇｉｂｃｏ，１５１４０１２２）を添加することで基本培地を作成した。基本培地とＩｎｔｅｓｔｉＣｕｌｔ Ｏｒｇａｎｏｉｄ Ｇｒｏｗｔｈ ＭｅｄｉｕｍのコンポーネントＡとを等量混合することで分化培地を作成した。９６ウェルプレートで単層化させた細胞に分化培地を１ｗｅｌｌあたり２００μＬずつ２日間隔で交換しながら計６日間分化を誘導した。
[Evaluation method of human norovirus inactivation effect]
(1) Culture of human Small Intestine Organoid (hSIO) hSIO was embedded in Matrigel (Corning, 356231) on a 24-well plate and cultured in 3D. The medium used was IntestiCult Organoid Growth Medium (Human) (STEMCELL Technologies, ST-06010). The procedures for medium exchange, passaging, and monolayering using a 96-well plate were according to the user's manual. For two days after trypsin treatment, CultureSure Y-27632 (Fujifilm Wako Pure Chemical Industries, Ltd.), a ROCK (Rho-associated coil forming kinase/Rho binding kinase) inhibitor, was added to the culture medium at a final concentration of 10 μM to inhibit anoikis. , 036-24023) was added. 500 mL of Advanced DMEM/F12 (Gibco, 12634010), 5 mL of GlutaMAX I (100X) (Gibco, 35050-061), 5 mL of 1M HEPES (Gibco, 15630080), 5 mL of Penicillin. -Addition of Streptomycin (Gibco, 15140122) A basic medium was created by doing this. A differentiation medium was prepared by mixing equal amounts of the basal medium and component A of IntestiCult Organoid Growth Medium. Differentiation was induced in the cells formed into a monolayer in a 96-well plate for a total of 6 days while replacing the differentiation medium at 200 μL per well every 2 days.
（２）ヒトノロウイルス含有糞便の１０％乳剤の作成
糞便の１０％乳剤はＧII．４型のヒトノロウイルス罹患者糞便から作成した。プロテアーゼ阻害剤であるｃＯｍｐｌｅｔｅ ｐｒｏｔｅａｓｅ ｉｎｈｉｂｉｔｏｒ ｃｏｃｋｔａｉｌ ｔａｂｌｅｔｓ（Ｓｉｇｍａ－Ａｌｄｒｉｃｈ，１１６９７４９８００１）１錠を５０ｍＬのＤ－ＰＢＳ（－）に懸濁した。糞便１ｇに対して１０ｍＬのｃＯｍｐｌｅｔｅ含有Ｄ－ＰＢＳ（－）で懸濁し、試験管ミキサーでよく混合した。４℃で２０分間静置した後に、２，０００×ｇ ４℃で１０分間遠心した。上清を新たなチューブに回収し、感染実験に供するまで－８０℃に保存した。
(2) Preparation of 10% emulsion of feces containing human norovirus A 10% emulsion of feces contains GII. It was prepared from the feces of a person infected with type 4 human norovirus. One tablet of cOmplete protease inhibitor cocktail tablets (Sigma-Aldrich, 11697498001) was suspended in 50 mL of D-PBS (-). 1 g of feces was suspended in 10 mL of cOmplete-containing D-PBS (-) and mixed well with a test tube mixer. After standing at 4°C for 20 minutes, the mixture was centrifuged at 2,000×g at 4°C for 10 minutes. The supernatant was collected into a new tube and stored at -80°C until used for infection experiments.
（３）ヒトノロウイルスの不活化処理と分化ｈＳＩＯへの感染
ヒトノロウイルス含有１０％糞便乳剤を分化培地で１０倍に希釈し、１ｍＬのシリンジとＭｉｌｌｅｘ ＨＶ Ｆｉｌｔｅｒ ｕｎｉｔ （Ｍｉｌｌｉｐｏｒｅ，ＳＬＨＶＲ０４ＮＬ）を用いて濾過した。ＰＡ微量遠心チューブ（Ｂｅｃｋｍａｎ ｃｏｕｌｔｅｒ，３５７４４８）中で濾過した糞便溶液５μＬ（２．８×１０６ ヒトノロウイルス ｇｅｎｏｍｅ ｃｏｐｙ相当）と表１に示す実施例１－１～１－９の各消毒剤組成物４５μＬとを混合し室温で、３０秒間反応させた。次いでこの薬剤処理された糞便溶液に、１ｍｇ／ｍＬのｂｏｖｉｎｅ ｓｅｒｕ ａｌｂｕｍｉｎ（Ｓｉｇｍａ－Ａｌｄｒｉｃｈ，Ａ９６４７）を含む基本培地 １．４５ｍＬを添加した。遠心チューブを固定角ロータＴＬＡ－５５（Ｂｅｃｋｍａｎ ｃｏｕｌｔｅｒ）にセットしＯｐｔｉｍａ ＭＡＸ－ＴＬ（Ｂｅｃｋｍａｎ ｃｏｕｌｔｅｒ）を用いてＲｍａｘにおいて１８６０４７×ｇの遠心力（５５，０００ｒｐｍ相当）で１．５時間超遠心した後に上清を除去した。ペレットを１００μＬの分化培地で懸濁し、ｈＳＩＯへの感染溶液とした。ウェル中の既存の培地を除去した６日間分化誘導後のｈＳＩＯに上述の方法で調製した感染溶液をアプライした。インキュベートは３７℃で１時間実施した。３００μＬの基本培地で３回洗浄した後に、分化培地を２５０μＬ添加し３７℃ ５％ＣＯ２の条件下でサンプリングのタイミングまで培養した。培養開始直後（ｄａｙ ０）と培養３日後（ｄａｙ ３）に１０μＬの上清を回収した。回収した上清はＲＴ－ｑＰＣＲに供するまで－８０℃で保存した。なお、薬剤溶液に代わり基本培地を用いて同様の操作を行い調製したｈＳＩＯへの感染溶液を薬剤非処理のコントロールとした。
(3) Inactivation treatment of human norovirus and infection of differentiated hSIO A 10% fecal emulsion containing human norovirus was diluted 10 times with differentiation medium and filtered using a 1 mL syringe and Millex HV Filter unit (Millipore, SLHVR04NL). . 5 μL of fecal solution (equivalent to 2.8×10 6 human norovirus genome copy) filtered in a PA microcentrifuge tube (Beckman Coulter, 357448) and each disinfectant composition of Examples 1-1 to 1-9 shown in Table 1. 45 μL and reacted at room temperature for 30 seconds. Then, 1.45 mL of basal medium containing 1 mg/mL bovine seroalbumin (Sigma-Aldrich, A9647) was added to the drug-treated fecal solution. The centrifuge tube was set in a fixed angle rotor TLA-55 (Beckman coulter) and ultracentrifuged for 1.5 hours at Rmax with a centrifugal force of 186,047 x g (equivalent to 55,000 rpm) using Optima MAX-TL (Beckman coulter). The supernatant was removed. The pellet was suspended in 100 μL of differentiation medium and used as an infection solution for hSIO. The infection solution prepared by the above method was applied to hSIO after differentiation induction for 6 days after removing the existing medium in the well. Incubation was performed at 37°C for 1 hour. After washing three times with 300 μL of basal medium, 250 μL of differentiation medium was added and cultured at 37° C. and 5% CO 2 until the timing of sampling. Immediately after the start of culture (day 0) and 3 days after culture (day 3), 10 μL of supernatant was collected. The collected supernatant was stored at -80°C until subjected to RT-qPCR. Note that a solution for infecting hSIO prepared by performing the same operation using a basic medium instead of the drug solution was used as a control without drug treatment.
（４）ＲＴ－ｑＰＣＲ
回収した上清中のヒトノロウイルス ｇｅｎｏｍｅ ｃｏｐｙ数の定量にはノロウイルス検出キット Ｇ１／Ｇ２（東洋紡，ＦＩＫ－２７３）を用いた。操作はプロトコールに従った。ＰＣＲ増幅とデータ測定はＬｉｇｈｔＣｙｃｌｅｒ４８０II（Ｒｏｃｈｅ）を用いた。
測定されたウイルス量に基づき、以下の基準に従って評価した。
Ａ：ヒトノロウイルスが検出下限にまで不活化されている。
Ｂ：ヒトノロウイルスを検出するがコントロール（薬剤処理無し）よりも減少している。
Ｃ：コントロール（薬剤処理無し）と同等にヒトノロウイルスが増殖している。
(4) RT-qPCR
Norovirus detection kit G1/G2 (Toyobo, FIK-273) was used to quantify the number of human norovirus genome copies in the collected supernatant. The operation followed the protocol. LightCycler 480II (Roche) was used for PCR amplification and data measurement.
Based on the measured viral load, evaluation was made according to the following criteria.
A: Human norovirus has been inactivated to the detection limit.
B: Human norovirus is detected, but it is reduced compared to the control (no drug treatment).
C: Human norovirus is proliferating at the same level as the control (no drug treatment).
実施例１－１～１－９の消毒剤組成物は、ヒトノロウイルス不活化効果の評価結果がすべてＡであり、十分なヒトノロウイルス不活化効果を有するものであった。 The disinfectant compositions of Examples 1-1 to 1-9 were all evaluated as A for their human norovirus inactivation effects, and had sufficient human norovirus inactivation effects.
Claims (15)
（Ａ）成分を６０質量％以上８０質量％以下、（Ｂ）成分を０．３０質量％以上０．８０質量％以下、（Ｃ）成分を０．０６質量％以上０．３０質量％以下、（Ｄ）成分を０．０２質量％以上０．１０質量％以下含有し、ｐＨが２５℃で９．５以上である消毒剤組成物を製造する、消毒剤組成物の製造方法。
(A) selected from ethanol [hereinafter referred to as component (A)], (B) potassium carbonate [hereinafter referred to as component (B)], (C) succinic acid, malic acid, fumaric acid, adipic acid, and salts thereof. Mixing one or more types [hereinafter referred to as component (C)] and (D) citric acid and/or its salt [hereinafter referred to as component (D)],
(A) component is 60% by mass or more and 80% by mass or less, (B) component is 0.30% by mass or more and 0.80% by mass or less, (C) component is 0.06% by mass or more and 0.30% by mass or less, (D) A method for producing a disinfectant composition, which comprises producing a disinfectant composition containing component (0.02% by mass or more and 0.10% by mass or less) and having a pH of 9.5 or more at 25°C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022073626A JP2023162913A (en) | 2022-04-27 | 2022-04-27 | disinfectant composition |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022073626A JP2023162913A (en) | 2022-04-27 | 2022-04-27 | disinfectant composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2023162913A true JP2023162913A (en) | 2023-11-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022073626A Pending JP2023162913A (en) | 2022-04-27 | 2022-04-27 | disinfectant composition |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2023162913A (en) |
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2022
- 2022-04-27 JP JP2022073626A patent/JP2023162913A/en active Pending
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