JP2023158607A - Anti-aging composition, and preparation method and application thereof - Google Patents
Anti-aging composition, and preparation method and application thereof Download PDFInfo
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- JP2023158607A JP2023158607A JP2022079212A JP2022079212A JP2023158607A JP 2023158607 A JP2023158607 A JP 2023158607A JP 2022079212 A JP2022079212 A JP 2022079212A JP 2022079212 A JP2022079212 A JP 2022079212A JP 2023158607 A JP2023158607 A JP 2023158607A
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Classifications
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
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Abstract
Description
本発明は、化粧品の分野に属し、抗老化用組成物、特に抗老化用組成物及び製造方法と応用に関する。 The present invention belongs to the field of cosmetics and relates to anti-aging compositions, in particular anti-aging compositions, manufacturing methods and applications.
ヒト皮膚の表皮組織及び細胞は、表皮のバリア作用により外部の侵襲から保護し得るだけでなく、さらに皮膚組織の水分蒸発を防ぐ保水又は保湿作用メカニズム等により、皮膚組織の老化進行を遅らせる作用を発揮することができる。皮膚の表皮組織のバリア構築と保湿・抗老化の作用は、有機体の生存と組織保護に非常に重要で、不可欠である。皮膚組織細胞は人体の他の細胞と同様に、時間の経過と有機体の加齢に伴い、構造、成分、機能等の老化と衰退も発生し、多くの代謝廃棄物、不活性化・老化した細胞、細胞下構造を効果的に除去することができず、常態と定常状態の生理学的細胞の不均衡により、皮膚の老化が加速され、病気にかかる可能性が向上する。 The epidermal tissues and cells of human skin not only protect from external invasion through the epidermal barrier effect, but also have the effect of slowing down the aging process of the skin tissue through water retention or moisturizing mechanisms that prevent water evaporation in the skin tissue. able to demonstrate. The barrier construction and moisturizing/antiaging effects of the epidermal tissue of the skin are extremely important and essential for the survival of organisms and tissue protection. Skin tissue cells, like other cells in the human body, undergo aging and decline in structure, composition, function, etc. with the passage of time and the aging of the organism, and produce many metabolic waste products, inactivation and aging. The inability to effectively remove damaged cells and subcellular structures, resulting in an imbalance between normal and steady-state physiological cells, accelerates skin aging and increases the susceptibility to disease.
老化は、有機体が性的成熟期を経た後、細胞の自己更新と修復能力が低下し、組織器官の構造と機能が退化し、最終的に死に至る過程であり、圧力ストレス能力が低下し、バランスが取られず、病気にかかるリスクが増加することを特徴とする。世界的な人口高齢化に伴い、様々な老年者退行性疾患及びそれによる巨大な医療費がますます深刻な社会的課題になってしまう。高齢者の健康状態を維持することは、人口高齢化に伴う社会的負担及び経済的負担を軽減する重要な環である。このため、簡便で容易に実行され、経済的且つ効果的であり、安全性が高く展開しやすい老化及び老化関連疾患に影響を与える措置及び方法を検討することに重要な意義を持っている。 Aging is a process in which, after an organism passes through a period of sexual maturity, the self-renewal and repair capacity of cells decreases, the structure and function of tissue organs degenerates, and ultimately leads to death, and the pressure stress capacity decreases. , characterized by an imbalance and an increased risk of contracting the disease. With the aging of the global population, various geriatric degenerative diseases and the huge medical costs associated with them are becoming increasingly serious social issues. Maintaining the health status of the elderly is an important link in reducing the social and economic burden associated with population aging. Therefore, it is of great significance to consider measures and methods to affect aging and aging-related diseases that are simple, easy to implement, economical, effective, safe and easy to develop.
今まで、抗老化用薬物の使用は一般的な抗老化手段である。臨床的に使用している老化を遅らせる薬物の多くは、細胞分裂を促進し、オキシラジカルの生成を抑えることができるビタミンＥ、細胞の寿命を長くできるプロカイン製剤、脳の老化等を遅らせることができるピラセタム、酸化ストレスに抵抗することで、老化による有機体の機能低下を抑えるアスピリンのような合成類薬物である。 Until now, the use of anti-aging drugs is a common anti-aging measure. Many of the drugs used clinically to delay aging include vitamin E, which can promote cell division and suppress the production of oxyradicals, procaine preparations, which can extend the lifespan of cells, and drugs that can delay brain aging. Piracetam is a synthetic aspirin-like drug that reduces the decline in the body's functions due to aging by resisting oxidative stress.
肌老化は、自然因子又は非自然因子による肌老化現象である。人が生まれた後、皮膚組織が日々に成長し、機能が徐々に活発になっており、ある年齢になると退化し始め、皮膚の老化が知らずに進んでいる。「お肌の曲がり角」と呼ばれる皮膚組織の成長期間が一般的に２５歳程度に終了し、この後、成長が老化と共に進行し、肌の弾性繊維が粗くなっていき、初老期になる４０－５０歳に、肌老化が明らかになるが、老化程度が人により異なる。肌老化に伴い、肌が薄くなりながら萎縮し、皮下脂肪が減少し、弾性繊維とコラーゲン繊維の変化により、肌の弾性と張力が徐々に失われ、さらに弛みとシワが発生する。 Skin aging is a skin aging phenomenon caused by natural or non-natural factors. After a person is born, the skin tissue grows day by day, its functions gradually become more active, and at a certain age it begins to degenerate, and the aging of the skin progresses without realizing it. The period of growth of skin tissue, called the "turning point of the skin," generally ends around the age of 25. After this, growth progresses with aging, and the elastic fibers of the skin become coarser, leading to the onset of old age.40- Skin aging becomes apparent at the age of 50, but the degree of aging differs from person to person. With skin aging, the skin becomes thinner and atrophic, subcutaneous fat decreases, and changes in elastic fibers and collagen fibers cause the skin to gradually lose its elasticity and tension, further causing sagging and wrinkles.
現在、医療美容・抗老化の分野において、フェイスリフト手術及び埋め込みスレッドリフトによるプチ整形手術以外、肌へのコラーゲン、エラスチン、及びヒアルロン酸の充填、又は、Ｔｈｅｒｍａｇｅ等の光電気治療、注射器注射等の美容整形治療等のような光、高周波電波で細胞に刺激を与え、コラーゲンの生成を促進する手段で線維芽細胞を活性化することを採用しているが、このように細胞を活性化するには、軽度の熱傷を受けて線維芽細胞の動作に刺激を与えなければならない。熱傷部の回復は主に線維芽細胞に依存し、線維芽細胞再生医療は、培養した線維芽細胞を真皮層に直接注入することで、再生細胞を直接補充し、細胞数を増加させ、分裂速度を上げ、若い肌状態に戻るように自体の肌にコラーゲン等の物質を生成させ続けることであり、効果が持続性を持ち、自然であり、継続的に培養して使用可能である。 Currently, in the field of medical beauty and anti-aging, in addition to facelift surgery and small plastic surgery using implanted thread lifts, there are treatments such as filling the skin with collagen, elastin, and hyaluronic acid, photoelectric treatments such as Thermage, syringe injections, etc. Similar to cosmetic surgery treatments, fibroblasts are activated by stimulating cells with light and high-frequency radio waves to promote collagen production. should stimulate fibroblast behavior after undergoing a mild burn injury. Recovery of burn areas mainly depends on fibroblasts, and fibroblast regenerative medicine injects cultured fibroblasts directly into the dermal layer to directly replenish regenerated cells, increase cell number, and stimulate cell division. It increases the speed and continues to produce substances such as collagen in the skin to return to a youthful skin state, and the effect is long-lasting, natural, and can be continuously cultivated and used.
年齢の増加に伴い、線維芽細胞の数が減少しているため、このような抗老化医療方法で、肌の表面に注射若しくはフェイスリフト又はスレッド埋め込みを複数回行う必要がある。 As the number of fibroblasts decreases with increasing age, such anti-aging medical methods require multiple injections or facelifts or thread implants on the skin surface.
現在、市場において肌表面用の抗老化用化粧品又はシワ改善用化粧品が現れているが、実用には、有効成分が肌を透過して備える生物学的効果を発揮しにくく、吸収が困難であり、シワ改善効果が緩慢に果たされ、効果が顕著でない等のような欠陥及び問題が存在する。 Currently, anti-aging cosmetics or wrinkle-improving cosmetics for the skin surface have appeared on the market, but in practical use, the active ingredients do not penetrate the skin to exert their biological effects and are difficult to absorb. There are deficiencies and problems such as the wrinkle improvement effect is slowly achieved and the effect is not significant.
したがって、皮膚線維芽細胞の増殖及びコラーゲンの生成を促進可能な組成物又は該組成物を含む製品が求められている。 Therefore, there is a need for compositions or products containing such compositions that can promote dermal fibroblast proliferation and collagen production.
本発明は、第１態様では、上記問題を解決するために、ヒト皮膚線維芽細胞の増殖及びコラーゲンの生成を促進し、抗老化用組成物の経皮吸収性能を向上させることができることで、シワが改善され、肌色が良くされ、損傷を受けた肌が修復され、乾燥肌が改善される抗老化用組成物を提供する。 In a first aspect of the present invention, in order to solve the above problems, the present invention can promote the proliferation of human skin fibroblasts and the production of collagen, and improve the transdermal absorption performance of an anti-aging composition. To provide an anti-aging composition that improves wrinkles, improves skin color, repairs damaged skin, and improves dry skin.
本発明は、第２態様では、上記抗老化用組成物の製造方法をさらに提供する。 In a second aspect, the present invention further provides a method for producing the above anti-aging composition.
本発明は、第３態様では、上記抗老化用組成物の応用をさらに提供する。 In a third aspect, the present invention further provides an application of the above anti-aging composition.
上記目的を達成するために、本発明は、以下の技術的解決手段を採用する。 In order to achieve the above object, the present invention adopts the following technical solutions.
本発明は、第１態様では、ナノリポソームと、前記ナノリポソームにロードされる、ジグルコシル没食子酸、グルコシルヘスペリジン、キバナオランダセンニチエキス、ビオチン、及びカフェインを含む活性成分と、を含む抗老化用組成物を提供する。 In a first aspect, the present invention provides an anti-aging drug comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the active ingredient including diglucosyl gallic acid, glucosyl hesperidin, Lacustilla chinensis extract, biotin, and caffeine. A composition is provided.
本発明では、皮膚の微生物層がα－グルコシダーゼを生成可能であり、ジグルコシル没食子酸が、α－グルコシダーゼの作用により、ジグルコシル没食子酸のグルコース結合を切断してトリヒドロキシ安息香酸を生成し、目的とするターゲットに迅速に作用することで、メラニン色素の生成が低減され、また、トリヒドロキシ安息香酸は、フリーラジカルを低減し、チロシナーゼの活性を抑える等の面で効果が高く、さらに、転化してメラニン色素を生成する酵素の発現を抑制し、メラニン色素の肌の表層への移動を抑制することができ、メラニン色素の移動抑制により、メラニン色素を減らす等の効果が達成される。ジグルコシル没食子酸は、チロシナーゼの活性を効果的に抑制し、メラニン色素の移動を防ぐことができ、それにより、明らかにシミが消され、肌色が明るくされ、白肌度が上げられ、また、ジグルコシル没食子酸は、さらに、高い抗フリーラジカル効果を示し、抗老化作用を有する。 In the present invention, the microbial layer of the skin is capable of producing α-glucosidase, and diglucosyl gallic acid cleaves the glucose bond of diglucosyl gallic acid to produce trihydroxybenzoic acid through the action of α-glucosidase. By quickly acting on the target, melanin pigment production is reduced, and trihydroxybenzoic acid is highly effective in reducing free radicals and inhibiting tyrosinase activity. It is possible to suppress the expression of the enzyme that produces melanin pigment and to suppress the movement of melanin pigment to the surface layer of the skin, and by suppressing the movement of melanin pigment, effects such as reducing melanin pigment can be achieved. Diglucosyl gallic acid can effectively suppress the activity of tyrosinase and prevent the movement of melanin pigment, thereby clearly erasing age spots, brightening the skin color, and increasing the whiteness of the skin. The acid furthermore shows a high anti-free radical effect and has an anti-aging effect.
グルコシルヘスペリジンは、柑橘皮から抽出された天然のバイオフラボノイドであり、水可溶性ヘスペリジンの誘導体である。血液循環を改善し、皮膚表面の循環を促進することにより、クマを改善することができ、グルコシルヘスペリジン中のフラボノイド化合物は、抗酸化、紫外線吸収及びフリーラジカル除去の効果を有するとともに、コラーゲン構造を効果的に強化することができる。 Glucosyl hesperidin is a natural bioflavonoid extracted from citrus peel and is a water-soluble derivative of hesperidin. By improving blood circulation and promoting circulation on the skin surface, it can improve dark circles, and the flavonoid compounds in glucosyl hesperidin have the effects of antioxidant, ultraviolet absorption and free radical scavenging, and also improve collagen structure. can be effectively strengthened.
キバナオランダセンニチエキスは、肌中のコラーゲンとラミニンの生成を促進し、顔面の筋収縮を低減し、顔面の小ジワ又はシワを低減し及び／又は防ぎ、顔面の重複する筋収縮による動的な顔面シワと額シワ（例えば、顔面シワ）を低減し、コラーゲンの生成を増加させ、マトリックスメタロプロテアーゼを低減し、ハリのある肌にし、並びに／又は、緩い、下垂、及び弛みのある肌状態を改善するために用いることができる。 Laminaria extract promotes the production of collagen and laminin in the skin, reduces facial muscle contractions, reduces and/or prevents fine lines or wrinkles on the face, and improves the dynamic appearance of facial redundant muscle contractions. reducing facial and forehead wrinkles (e.g., facial wrinkles), increasing collagen production, reducing matrix metalloproteinases, firming skin, and/or loose, drooping, and lax skin conditions. can be used to improve
ビオチンは、ビタミンＨ、コエンザイムＲとも呼ばれ、ビタミンＢ族、Ｂ７にも該当する水溶性ビタミンである。それは、ビタミンＣの合成に必要なものであり、脂肪とタンパク質の正常な代謝に不可欠なものである。人体の自然な成長、発育及び正常な機能的健康の維持に必要な栄養素でもある。ビオチンは美容効果に優れ、透き通るように白くきめ細かい肌を保持することができる。 Biotin is also called vitamin H or coenzyme R, and is a water-soluble vitamin that also falls under vitamin B group B7. It is required for the synthesis of vitamin C, which is essential for the normal metabolism of fats and proteins. It is also an essential nutrient for the natural growth, development and maintenance of normal functional health of the human body. Biotin has excellent beauty effects and can maintain clear, white, and fine-textured skin.
カフェインは、白色の粉末状であり、特徴的な匂いがし、細胞の新陳代謝を促進し、局所的な微小循環を加速する作用を有する。 Caffeine is a white powder with a characteristic odor and has the effect of promoting cellular metabolism and accelerating local microcirculation.
カフェイン、ビオチン、キバナオランダセンニチエキス、グルコシルヘスペリジン、及びジグルコシル没食子酸を配合してなる活性成分は、肌に効果的に吸収され、メラニン色素の沈着を効果的に低減し、外部侵襲及びフリーラジカルに抵抗し、且つ炎症性因子の活性を効果的に抑え、肌の水分流出を低減することができ、それにより、目のくまを効果的に改善し、消し、肌老化を遅らせ、肌の光沢を上げる。 The active ingredients, which are blended with caffeine, biotin, Laminaria japonica extract, glucosyl hesperidin, and diglucosyl gallic acid, are effectively absorbed into the skin and effectively reduce melanin pigment deposition, making it free from external invasion. It can resist radicals and effectively suppress the activity of inflammatory factors, reduce skin water loss, thereby effectively improving and erasing dark circles, delaying skin aging, and improving skin Increase gloss.
さらに、ナノリポソームに活性成分をロードすれば、安定性が高く、優しく、肌への刺激性が低く、製造過程での活性成分の構造破壊を回避する。 Furthermore, loading active ingredients into nanoliposomes is highly stable, gentle, and less irritating to the skin, avoiding structural destruction of active ingredients during the manufacturing process.
本発明の好適な解決手段として、前記抗老化用組成物は、重量パーセントで、０．０１１％－１．１％のジグルコシル没食子酸、０．０１％－１．５％のグルコシルヘスペリジン、０．０１％－０．８％のキバナオランダセンニチエキス、０．００００１％－０．０００５％のビオチン、０．００００１％－０．０００５％のカフェイン、０．６％－２．６％のコレステロール、０．１％－１．５％のベヘニルアルコール、６％－６．５％の水添レシチン、及び残部の脱イオン水を含む。 As a preferred solution of the invention, the anti-aging composition comprises, in weight percentages, 0.011%-1.1% diglucosyl gallic acid, 0.01%-1.5% glucosyl hesperidin, 0. 0.01% - 0.8% Laminaria extract, 0.00001% - 0.0005% Biotin, 0.00001% - 0.0005% Caffeine, 0.6% - 2.6% Cholesterol , 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the balance deionized water.
本発明の好適な解決手段として、前記抗老化用組成物は、重量パーセントで、０．０１１％－０．５％のジグルコシル没食子酸、０．０１％－０．５％のグルコシルヘスペリジン、０．０４４７％－０．６％のキバナオランダセンニチエキス、０．００００１％－０．０００５％のビオチン、０．００００１％－０．０００５％のカフェイン、０．６％－２．６％のコレステロール、０．１％－１．５％のベヘニルアルコール、６％－６．５％の水添レシチン、及び残部の脱イオン水を含む。 As a preferred solution of the present invention, the anti-aging composition comprises, in weight percentages, 0.011%-0.5% diglucosyl gallic acid, 0.01%-0.5% glucosyl hesperidin, 0. 0447% - 0.6% Laminaria japonica extract, 0.00001% - 0.0005% biotin, 0.00001% - 0.0005% caffeine, 0.6% - 2.6% cholesterol , 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the balance deionized water.
本発明の好適な解決手段として、前記抗老化用組成物は、重量パーセントで、０．０１１％のジグルコシル没食子酸、０．５％のグルコシルヘスペリジン、０．０４４７％のキバナオランダセンニチエキス、０．０００１％のビオチン、０．０００１％のカフェイン、０．６％－２．６％のコレステロール、０．１％－１．５％のベヘニルアルコール、６％－６．５％の水添レシチン、及び残部の脱イオン水を含む。 As a preferred solution of the present invention, the anti-aging composition comprises, in weight percent, 0.011% diglucosyl gallic acid, 0.5% glucosyl hesperidin, 0.0447% Lacustnia chinensis extract, 0. .0001% biotin, 0.0001% caffeine, 0.6%-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the remainder deionized water.
本発明の好適な解決手段として、前記ナノリポソームの粒径が１００－２５０ｎｍである。 As a preferred solution of the present invention, the particle size of the nanoliposomes is 100-250 nm.
本発明は、第２態様では、
ａ）活性成分とナノリポソームを溶解し、混合溶液を得る工程と、
ｂ）工程ａ）の混合溶液中の溶媒を蒸発させて除去し、４０－６０℃で、脱イオン水を加え、１５－３５Ｈｚの超音波周波数で超音波処理を１５－２０分行い、超音波処理を完了した後、濾過して抗老化用組成物を得る工程と、を含む上記抗老化用組成物の製造方法を提供する。
In a second aspect of the present invention,
a) dissolving the active ingredient and nanoliposome to obtain a mixed solution;
b) Remove the solvent in the mixed solution of step a) by evaporation, add deionized water at 40-60 °C, and perform ultrasonic treatment for 15-20 minutes at an ultrasonic frequency of 15-35 Hz. After completing the treatment, the method for producing the anti-aging composition is provided, which includes the step of filtering to obtain the anti-aging composition.
本発明は、第３態様では、上記抗老化用組成物の化粧品製造への応用を提供し、前記化粧品は、化粧水、フェイスパック、エッセンス、アイクリーム、アイパック、スキンケアゲル、スキンケア乳液、フェイスクリーム、又はスプレーを含む。 In a third aspect, the present invention provides an application of the above-mentioned anti-aging composition to the production of cosmetics, and the cosmetics include lotion, face pack, essence, eye cream, eye pack, skin care gel, skin care emulsion, face Contains cream or spray.
本発明の好適な解決手段として、前記化粧品には、溶媒、増粘剤、防腐剤、ｐＨ調整剤、保湿剤、エモリエント剤、キレート剤、乳化剤、精油、香料、色素のうちの少なくとも１つを含む添加物がさらに含まれる。 As a preferred solution of the present invention, the cosmetic product contains at least one of a solvent, a thickener, a preservative, a pH adjuster, a humectant, an emollient, a chelating agent, an emulsifier, an essential oil, a fragrance, and a pigment. Further included are additives that include.
本発明の好適な解決手段として、前記化粧品は、重量部で、抗老化用組成物０．０１－６０部、水添ナタネ油アルコール０．１－０．８部、リンゴ酸ジイソステアリル５－１０部、１，３－プロパンジオール５－１０部、カプリル酸ヤシ油アルキル３－１２部、マカデミアナッツ脂肪酸フィトステリル０．０１－０．１部、パルミチン酸レチノール０．０１－２部、ヘスペリジンメチルカルコン０．０１－０．５部及び脱イオン水５０－８０部を含む、アイクリームである。 As a preferred solution of the present invention, the cosmetic product comprises, in parts by weight, 0.01-60 parts of the anti-aging composition, 0.1-0.8 parts of hydrogenated rapeseed oil alcohol, 5-diisostearyl malate. 10 parts, 1,3-propanediol 5-10 parts, coconut oil alkyl caprylate 3-12 parts, macadamia nut fatty acid phytosteryl 0.01-0.1 part, retinol palmitate 0.01-2 parts, hesperidin methyl chalcone 0 0.01-0.5 parts and 50-80 parts deionized water.
本発明の好適な解決手段として、前記アイクリームの製造方法は、
１）水添ナタネ油アルコール、リンゴ酸ジイソステアリル、パルミチン酸レチノール、カプリル酸ヤシ油アルキル、マカデミアナッツ脂肪酸フィトステリル、及びヘスペリジンメチルカルコンを加えて均一に撹拌し、５０－９０℃に加熱して均一に撹拌し、油相とする工程と、
２）抗老化用組成物、水、及び１，３－プロパンジオールを均一に混合して撹拌し、水相とする工程と、
３）工程１）で得られた油相と工程２）で得られた水相を混合し、得られた混合物を超臨界装置の反応釜内に入れ、超臨界装置の反応釜内の温度が４５－６５℃になるように二酸化炭素を注入して調整し、圧力を１２－１５Ｍｐａに、時間を３０－４５分に設定し、減圧して二酸化炭素を放出し、室温まで冷却すると、アイクリームを得る工程と、を含む。
As a preferred solution of the present invention, the method for producing the eye cream includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, coconut oil alkyl caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methylchalcone, stir evenly, and heat to 50-90°C to make a uniform mixture. Stirring to form an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction pot of the supercritical device, and raise the temperature inside the reaction pot of the supercritical device. Adjust the temperature by injecting carbon dioxide to 45-65℃, set the pressure to 12-15Mpa and time to 30-45 minutes, reduce the pressure to release carbon dioxide, cool to room temperature, and make an eye cream. and a step of obtaining.
従来技術と比較して、本発明は以下の有益な効果を有する。 Compared with the prior art, the present invention has the following beneficial effects.
１）本発明は、カフェイン、ビオチン、キバナオランダセンニチエキス、グルコシルヘスペリジン、及びジグルコシル没食子酸を配合して活性成分にし、さらにナノリポソームで活性成分をロードさせることで、安定性が高く、優しく、肌への刺激性が低く、製造過程での活性成分の構造破壊を回避し、肌により効果的に吸収され、メラニン色素の沈着を効果的に低減し、外部侵襲及びフリーラジカルに抵抗し、且つ炎症性因子の活性を効果的に抑え、肌の水分流出を低減することができ、それにより目のくまを効果的に改善し、消し、肌老化を遅らせ、肌の光沢を上げる。 1) The present invention is highly stable and gentle by blending caffeine, biotin, Laminaria chinensis extract, glucosyl hesperidin, and diglucosyl gallic acid as active ingredients, and loading the active ingredients with nanoliposomes. , has low irritation to the skin, avoids structural destruction of active ingredients during the manufacturing process, is more effectively absorbed by the skin, effectively reduces melanin pigmentation, resists external invasion and free radicals, It can also effectively suppress the activity of inflammatory factors and reduce skin water loss, thereby effectively improving and erasing dark circles, delaying skin aging, and increasing skin gloss.
２）本発明の抗老化用組成物は、皮膚線維芽細胞の増殖及びコラーゲンの生成を促進することができ、それにより、シワが改善され、肌色が良くされ、損傷を受けた肌が修復され、乾燥肌が改善される。 2) The anti-aging composition of the present invention can promote the proliferation of skin fibroblasts and the production of collagen, thereby improving wrinkles, improving skin tone, and repairing damaged skin. , dry skin is improved.
また、本発明をよりよく説明するために、以下の発明を実施するための形態において多くの具体的な詳細が示されている。当業者であれば理解できるように、特定の具体的な詳細がなくても、本発明は同様に実施可能である。別のいくつかの実例において、本発明の趣旨を顕在化するために、当業者に熟知の方法、手段、機器及び工程が詳しく説明されていない。 In addition, numerous specific details are set forth in the following detailed description to better explain the invention. As will be understood by those skilled in the art, the invention may equally be practiced without the specific details. In other instances, methods, means, equipment and steps that are familiar to those skilled in the art have not been described in detail in order to obscure the spirit of the invention.
説明しておきたいのは、本明細書において、「ことができる」又は「可能」の使用は、特定の処理をすること及び特定の処理をしないという両方が意味する。本明細書において、「値Ａ－値Ｂ」で示される値の範囲とは、エンドポイント値Ａ、Ｂを含む範囲を指すということである。 It should be explained that in this specification, the use of "capable" or "possible" means both performing a certain process and not performing a certain process. In this specification, the value range indicated by "value A-value B" refers to a range that includes endpoint values A and B.
特別な声明がない限り、本発明で使用される単位はいずれも国際単位とし、且つ本発明に現れる値、値の範囲はいずれも工業生産に許容可能な誤差を含むと理解すべきである。 Unless otherwise specified, all units used in the present invention are international units, and it should be understood that all values and ranges of values appearing in the present invention include tolerances for industrial production.
ジグルコシル没食子酸、グルコシルヘスペリジン、キバナオランダセンニチエキス、ビオチン、カフェインのような本発明に用いられる原料は、いずれも市場から購入されるものである。 All of the raw materials used in the present invention, such as diglucosyl gallic acid, glucosyl hesperidin, Laburnum orandascenchi extract, biotin, and caffeine, are purchased from the market.
使用されるキバナオランダセンニチエキス（Ａｃｍｅｌｌａ ｏｌｅｒａｃｅａ ｅｘｔｒａｃｔ）は水－アルコール抽出物であり、溶媒は、水、エタノールと１，３－プロパンジオールの液体混合物を含み、得られた抽出液からエタノールが除去される。 The Acmella oleracea extract used is a water-alcoholic extract, the solvent comprises a liquid mixture of water, ethanol and 1,3-propanediol, and the ethanol is removed from the resulting extract. be done.
本発明では、化粧品に用いられる添加物は、溶媒、増粘剤、防腐剤、ｐＨ調整剤、保湿剤、エモリエント剤、キレート剤、乳化剤、精油、香料、色素のような従来の化粧品市場によく用いられる添加物であってもよい。 In the present invention, additives used in cosmetics are commonly used in the conventional cosmetics market, such as solvents, thickeners, preservatives, pH regulators, humectants, emollients, chelating agents, emulsifiers, essential oils, fragrances, and pigments. It may also be an additive used.
実施例１
本実施例は、ナノリポソームと前記ナノリポソームにロードされる活性成分を含む抗老化用組成物を提供し、抗老化用組成物は、重量パーセントで、０．０１１％のジグルコシル没食子酸、０．５％のグルコシルヘスペリジン、０．０４４７％のキバナオランダセンニチエキス、０．０００１％のビオチン、０．０００１％のカフェイン、１．８％のコレステロール、１．２％のベヘニルアルコール、６．３％の水添レシチン、及び残部の脱イオン水を含む。
Example 1
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percentages, 0.011% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 5% glucosyl hesperidin, 0.0447% Lacustilla chinensis extract, 0.0001% biotin, 0.0001% caffeine, 1.8% cholesterol, 1.2% behenyl alcohol, 6.3% of hydrogenated lecithin, and the balance of deionized water.
抗老化用組成物の製造方法は、
ａ）混合比で、ジグルコシル没食子酸、グルコシルヘスペリジン、キバナオランダセンニチエキス、ビオチン、カフェイン、コレステロール、ベヘニルアルコール、及び水添レシチンを無水エタノールに溶解し、混合溶液を得る工程と、
ｂ）工程ａ）の混合溶液中の無水エタノールを蒸発させて除去し、４０℃で、脱イオン水を加え、１５Ｈｚ超音波周波数で超音波処理を１５分行い、超音波処理を完了した後、濾過して抗老化用組成物を得る工程と、を含む。
The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Evaporate and remove the anhydrous ethanol in the mixed solution of step a), add deionized water at 40 ° C., perform ultrasound treatment at 15 Hz ultrasound frequency for 15 minutes, and after completing the ultrasound treatment, and filtering to obtain an anti-aging composition.
実施例２
本実施例は、ナノリポソームと前記ナノリポソームにロードされる活性成分を含む抗老化用組成物を提供し、抗老化用組成物は、重量パーセントで、１．１％のジグルコシル没食子酸、１．５％のグルコシルヘスペリジン、０．８％のキバナオランダセンニチエキス、０．０００５％のビオチン、０．０００５％のカフェイン、２．６％のコレステロール、１．５％のベヘニルアルコール、６．５％の水添レシチン、及び残部の脱イオン水を含む。
Example 2
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percent, 1.1% diglucosyl gallic acid; 1.1% diglucosyl gallic acid; 5% glucosyl hesperidin, 0.8% Lavender chinensis extract, 0.0005% biotin, 0.0005% caffeine, 2.6% cholesterol, 1.5% behenyl alcohol, 6.5% of hydrogenated lecithin, and the balance of deionized water.
抗老化用組成物の製造方法は、
ａ）混合比で、ジグルコシル没食子酸、グルコシルヘスペリジン、キバナオランダセンニチエキス、ビオチン、カフェイン、コレステロール、ベヘニルアルコール、及び水添レシチンを無水エタノールに溶解し、混合溶液を得る工程と、
ｂ）工程ａ）の混合溶液中の無水エタノールを蒸発させて除去し、５０℃で、脱イオン水を加え、２５Ｈｚ超音波周波数で超音波処理を１８分行い、超音波処理を完了した後、濾過して抗老化用組成物を得る工程と、を含む。
The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Evaporate and remove the anhydrous ethanol in the mixed solution of step a), add deionized water at 50 °C, and perform ultrasonic treatment at 25 Hz ultrasonic frequency for 18 minutes, after completing the ultrasonic treatment, and filtering to obtain an anti-aging composition.
実施例３
本実施例は、ナノリポソームと前記ナノリポソームにロードされる活性成分を含む抗老化用組成物を提供し、抗老化用組成物は、重量パーセントで、０．０１１％のジグルコシル没食子酸、０．０１％のグルコシルヘスペリジン、０．０１％のキバナオランダセンニチエキス、０．００００１％のビオチン、０．００００１％のカフェイン、０．６％のコレステロール、０．１％のベヘニルアルコール、６％の水添レシチン、及び残部の脱イオン水を含む。
Example 3
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percentages, 0.011% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% glucosyl hesperidin, 0.01% Lacustilla chinensis extract, 0.00001% biotin, 0.00001% caffeine, 0.6% cholesterol, 0.1% behenyl alcohol, 6% water Contains added lecithin and the balance deionized water.
抗老化用組成物の製造方法は、
ａ）混合比で、ジグルコシル没食子酸、グルコシルヘスペリジン、キバナオランダセンニチエキス、ビオチン、カフェイン、コレステロール、ベヘニルアルコール、及び水添レシチンを無水エタノールに溶解し、混合溶液を得る工程と、
ｂ）工程ａ）の混合溶液中の無水エタノールを蒸発させて除去し、６０℃で、脱イオン水を加え、１５－３５Ｈｚの超音波周波数で超音波処理を１５－２０分行い、超音波処理を完了した後、濾過して抗老化用組成物を得る工程と、を含む。
The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Evaporate and remove the absolute ethanol in the mixed solution of step a), add deionized water at 60°C, and perform ultrasonic treatment at an ultrasonic frequency of 15-35 Hz for 15-20 minutes. and filtering to obtain an anti-aging composition.
実施例４
本実施例は、ナノリポソームと前記ナノリポソームにロードされる活性成分を含む抗老化用組成物を提供し、抗老化用組成物は、重量パーセントで、０．０１１％のジグルコシル没食子酸、１．２％のグルコシルヘスペリジン、０．６％のキバナオランダセンニチエキス、０．０００２％のビオチン、０．００００７８％のカフェイン、１．８％のコレステロール、０．９％のベヘニルアルコール、６．２％の水添レシチン、及び残部の脱イオン水を含む。
Example 4
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percent, 0.011% diglucosyl gallic acid, 1. 2% glucosyl hesperidin, 0.6% Lavender chinensis extract, 0.0002% biotin, 0.000078% caffeine, 1.8% cholesterol, 0.9% behenyl alcohol, 6.2% of hydrogenated lecithin, and the balance of deionized water.
抗老化用組成物の製造方法は、
ａ）混合比で、ジグルコシル没食子酸、グルコシルヘスペリジン、キバナオランダセンニチエキス、ビオチン、カフェイン、コレステロール、ベヘニルアルコール、及び水添レシチンを無水エタノールに溶解し、混合溶液を得る工程と、
ｂ）工程ａ）の混合溶液中の溶媒を蒸発させて除去し、４５℃で、脱イオン水を加え、１５Ｈｚ超音波周波数で超音波処理を２０分行い、超音波処理を完了した後、濾過して抗老化用組成物を得る工程と、を含む。
The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Remove the solvent in the mixed solution of step a) by evaporation, add deionized water at 45 °C, perform ultrasonic treatment at 15 Hz ultrasonic frequency for 20 minutes, and after completing the ultrasonic treatment, filter. and obtaining an anti-aging composition.
実施例５
本実施例は、ナノリポソームと前記ナノリポソームにロードされる活性成分を含む抗老化用組成物を提供し、抗老化用組成物は、重量パーセントで、０．４３％のジグルコシル没食子酸、１．１２％のグルコシルヘスペリジン、０．０４４７％のキバナオランダセンニチエキス、０．０００１％のビオチン、０．０００４％のカフェイン、０．９％のコレステロール、１．１％のベヘニルアルコール、６％の水添レシチン、及び残部の脱イオン水を含む。
Example 5
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percent, 0.43% diglucosyl gallic acid, 1. 12% glucosyl hesperidin, 0.0447% Lacustilla dianthus extract, 0.0001% biotin, 0.0004% caffeine, 0.9% cholesterol, 1.1% behenyl alcohol, 6% water Contains added lecithin and the balance deionized water.
抗老化用組成物の製造方法は、
ａ）混合比で、ジグルコシル没食子酸、グルコシルヘスペリジン、キバナオランダセンニチエキス、ビオチン、カフェイン、コレステロール、ベヘニルアルコール、及び水添レシチンを無水エタノールに溶解し、混合溶液を得る工程と、
ｂ）工程ａ）の混合溶液中の無水エタノールを蒸発させて除去し、在５５℃で、脱イオン水を加え、３５Ｈｚ超音波周波数で超音波処理を１５分行い、超音波処理を完了した後、濾過して抗老化用組成物を得る工程と、を含む。
The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Remove the anhydrous ethanol in the mixed solution of step a) by evaporation, add deionized water at 55 ° C, and perform ultrasonic treatment at 35 Hz ultrasonic frequency for 15 minutes, after completing the ultrasonic treatment. and filtering to obtain an anti-aging composition.
実施例６
本実施例は、重量部で、実施例１で得られた抗老化用組成物１２．５部、水添ナタネ油アルコール０．５部、リンゴ酸ジイソステアリル５部、１，３－プロパンジオール１０部、カプリル酸ヤシ油アルキル１０部、マカデミアナッツ脂肪酸フィトステリル０．０５部、パルミチン酸レチノール０．５部、ヘスペリジンメチルカルコン０．２部、及び脱イオン水８０部を含むシワ改善用アイクリームを提供する。
Example 6
This example shows, in parts by weight, 12.5 parts of the anti-aging composition obtained in Example 1, 0.5 parts of hydrogenated rapeseed oil alcohol, 5 parts of diisostearyl malate, and 1,3-propanediol. 10 parts of coconut oil alkyl caprylate, 0.05 part of macadamia nut fatty acid phytosteryl, 0.5 part of retinol palmitate, 0.2 part of hesperidin methyl chalcone, and 80 parts of deionized water. do.
該シワ改善用アイクリームの製造方法は、
１）水添ナタネ油アルコール、リンゴ酸ジイソステアリル、パルミチン酸レチノール、カプリル酸ヤシ油アルキル、マカデミアナッツ脂肪酸フィトステリル（ＰＨＹＴＯＳＴＥＲＹＬ ＭＡＣＡＤＡＭＩＡＴＥ、アオケ新材料科技（上海）有限公司から購入される（訳注：「アオ」は「敖」の下に「山」、「ケ」は「禾」の右に「果」）、及びヘスペリジンメチルカルコン（ヘスペリジンメチルカルコンが複数のサプライヤーから購入可能な化合物である（国際化粧品成分辞書・ハンドブック、第１２版、第１巻、第１１４６ページ（２００８）を参照、その内容が引用により本書に組み込まれる）。一方で、該成分はＥｙｅｌｉｓｓＴＭの商品名で販売されるＳｅｄｅｒｍａ ＳＡＳ（フランス）混合物の使用により取得可能であり、該混合物は水、グリセロール、ヘスペリジンメチルカルコン、ステアレス－２０、ジペプチド－２（バリン－トリプトファン）、及びパルミトイルテトラペプチド－７（Ｐａｌ－ＧＱＰＲ）の組み合わせである）を加えて均一に撹拌し、５０℃に加熱して均一に撹拌し、油相とする工程と、
２）抗老化用組成物、水、及び１，３－プロパンジオールを均一に混合して撹拌し、水相とする工程と、
３）工程１）で得られた油相と工程２）で得られた水相を混合し、得られた混合物を超臨界装置の反応釜内に入れ、超臨界装置反応釜内の温度が５５℃になるように二酸化炭素を注入して調整し、圧力を１２Ｍｐａに、時間を３０分に設定し、減圧して二酸化炭素を放出し、室温まで冷却すると、アイクリームを得る工程と、を含む。
The method for producing the eye cream for wrinkle improvement includes:
1) Hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, coconut oil alkyl caprylate, PHYTOSTERYL MACADAMIATE, purchased from Aoke New Material Technology (Shanghai) Co., Ltd. is a compound that can be purchased from multiple suppliers (International Cosmetic Ingredient Dictionary). - Handbook, 12th Edition, Volume 1, Page 1146 (2008), the contents of which are incorporated herein by reference).On the other hand, the ingredient is Sederma SAS (France), sold under the trade name EyelissTM. can be obtained by using a mixture, which is a combination of water, glycerol, hesperidin methyl chalcone, steareth-20, dipeptide-2 (valine-tryptophan), and palmitoyltetrapeptide-7 (Pal-GQPR). Adding and stirring uniformly, heating to 50 ° C. and stirring uniformly to form an oil phase,
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction vessel of a supercritical apparatus, and bring the temperature inside the reaction vessel of the supercritical apparatus to 55. ℃, set the pressure to 12Mpa and time to 30 minutes, reduce the pressure to release carbon dioxide, and cool to room temperature to obtain an eye cream. .
実施例７
本実施例は、重量部で、実施例１で得られる抗老化用組成物２５部、水添ナタネ油アルコール０．８部、リンゴ酸ジイソステアリル１０部、１，３－プロパンジオール５部、カプリル酸ヤシ油アルキル３部、マカデミアナッツ脂肪酸フィトステリル０．０１部、パルミチン酸レチノール０．０１部、ヘスペリジンメチルカルコン０．０１部及び脱イオン水５０部を含むシワ改善用アイクリームを提供する。
Example 7
This example includes, in parts by weight, 25 parts of the anti-aging composition obtained in Example 1, 0.8 parts of hydrogenated rapeseed oil alcohol, 10 parts of diisostearyl malate, 5 parts of 1,3-propanediol, To provide an eye cream for improving wrinkles, which contains 3 parts of coconut oil alkyl caprylate, 0.01 part of macadamia nut fatty acid phytosteryl, 0.01 part of retinol palmitate, 0.01 part of hesperidin methyl chalcone, and 50 parts of deionized water.
該シワ改善用アイクリームの製造方法は、
１）水添ナタネ油アルコール、リンゴ酸ジイソステアリル、パルミチン酸レチノール、カプリル酸ヤシ油アルキル、マカデミアナッツ脂肪酸フィトステリル、及びヘスペリジンメチルカルコンを加えて均一に撹拌し、９０℃に加熱して均一に撹拌し、油相とする工程と、
２）抗老化用組成物、水、及び１，３－プロパンジオールを均一に混合して撹拌し、水相とする工程と、
３）工程１）で得られた油相と工程２）で得られた水相を混合し、得られた混合物を超臨界装置の反応釜内に入れ、超臨界装置反応釜内の温度が６５℃になるように二酸化炭素を注入して調整し、圧力を１４Ｍｐａに、時間を３０分に設定し、減圧して二酸化炭素を放出し、室温まで冷却すると、アイクリームを得る工程と、を含む。
The method for producing the eye cream for wrinkle improvement includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, alkyl coconut oil caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methyl chalcone, stir uniformly, heat to 90°C, and stir uniformly. , a step of forming an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction vessel of a supercritical device, and bring the temperature inside the supercritical device reaction vessel to 65%. ℃, set the pressure to 14 MPa and time to 30 minutes, reduce the pressure to release carbon dioxide, and cool to room temperature to obtain eye cream. .
実施例８
本実施例は、重量部で、実施例１で得られる抗老化用組成物５０部、水添ナタネ油アルコール０．１部、リンゴ酸ジイソステアリル５部、１，３－プロパンジオール７部、カプリル酸ヤシ油アルキル１２部、マカデミアナッツ脂肪酸フィトステリル０．１部、パルミチン酸レチノール２部、ヘスペリジンメチルカルコン０．５部、及び脱イオン水７５部を含むシワ改善用アイクリームを提供する。
Example 8
This example includes, in parts by weight, 50 parts of the anti-aging composition obtained in Example 1, 0.1 part of hydrogenated rapeseed oil alcohol, 5 parts of diisostearyl malate, 7 parts of 1,3-propanediol, To provide an eye cream for improving wrinkles, comprising 12 parts of coconut oil alkyl caprylate, 0.1 part of macadamia nut fatty acid phytosteryl, 2 parts of retinol palmitate, 0.5 part of hesperidin methyl chalcone, and 75 parts of deionized water.
該シワ改善用アイクリームの製造方法は、
１）水添ナタネ油アルコール、リンゴ酸ジイソステアリル、パルミチン酸レチノール、カプリル酸ヤシ油アルキル、マカデミアナッツ脂肪酸フィトステリル、及びヘスペリジンメチルカルコンを加えて均一に撹拌し、８０℃に加熱して均一に撹拌し、油相とする工程と、
２）抗老化用組成物、水及び１，３－プロパンジオールを均一に混合して撹拌し、水相とする工程と、
３）工程１）で得られた油相と工程２）で得られた水相を混合し、得られた混合物を超臨界装置の反応釜内に入れ、超臨界装置反応釜内の温度が４５℃になるように二酸化炭素を注入して調整し、圧力を１５Ｍｐａに、時間を４０分に設定し、減圧して二酸化炭素を放出し、室温まで冷却すると、アイクリームを得る工程と、を含む。
The method for producing the eye cream for wrinkle improvement includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, alkyl coconut oil caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methyl chalcone, stir evenly, heat to 80°C, and stir evenly. , a step of forming an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water and 1,3-propanediol to form an aqueous phase;
3) The oil phase obtained in step 1) and the aqueous phase obtained in step 2) are mixed, and the resulting mixture is placed in the reaction vessel of a supercritical device until the temperature inside the supercritical device reaction vessel reaches 45%. ℃, set the pressure to 15 MPa and time to 40 minutes, reduce the pressure to release carbon dioxide, and cool to room temperature to obtain an eye cream. .
実施例９
本実施例は、重量部で、実施例１で得られる抗老化用組成物１００部、水添ナタネ油アルコール０．１部、リンゴ酸ジイソステアリル５部、１，３－プロパンジオール７部、カプリル酸ヤシ油アルキル１２部、マカデミアナッツ脂肪酸フィトステリル０．１部、パルミチン酸レチノール２部、ヘスペリジンメチルカルコン０．５部、及び脱イオン水７５部を含むシワ改善用アイクリームを提供する。
Example 9
This example includes, in parts by weight, 100 parts of the anti-aging composition obtained in Example 1, 0.1 part of hydrogenated rapeseed oil alcohol, 5 parts of diisostearyl malate, 7 parts of 1,3-propanediol, To provide an eye cream for improving wrinkles, comprising 12 parts of coconut oil alkyl caprylate, 0.1 part of macadamia nut fatty acid phytosteryl, 2 parts of retinol palmitate, 0.5 part of hesperidin methyl chalcone, and 75 parts of deionized water.
該シワ改善用アイクリームの製造方法は、
１）水添ナタネ油アルコール、リンゴ酸ジイソステアリル、パルミチン酸レチノール、カプリル酸ヤシ油アルキル、マカデミアナッツ脂肪酸フィトステリル、及びヘスペリジンメチルカルコンを加えて均一に撹拌し、８０℃に加熱して均一に撹拌し、油相とする工程と、
２）抗老化用組成物、水及び１，３－プロパンジオールを均一に混合して撹拌し、水相とする工程と、
３）工程１）で得られた油相と工程２）で得られた水相を混合し、得られた混合物を超臨界装置の反応釜内に入れ、超臨界装置反応釜内の温度が４５℃になるように二酸化炭素を注入して調整し、圧力を１５Ｍｐａに、時間を４０分に設定し、減圧して二酸化炭素を放出し、室温まで冷却すると、アイクリームを得る工程と、を含む。
The method for producing the eye cream for wrinkle improvement includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, alkyl coconut oil caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methyl chalcone, stir evenly, heat to 80°C, and stir evenly. , a step of forming an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water and 1,3-propanediol to form an aqueous phase;
3) The oil phase obtained in step 1) and the aqueous phase obtained in step 2) are mixed, and the resulting mixture is placed in the reaction vessel of a supercritical device until the temperature inside the supercritical device reaction vessel reaches 45%. ℃, set the pressure to 15 MPa and time to 40 minutes, reduce the pressure to release carbon dioxide, and cool to room temperature to obtain an eye cream. .
実施例１で得られる抗老化用組成物について、細胞増殖促進効果試験及びコラーゲン生成効果試験を行う。 The anti-aging composition obtained in Example 1 is subjected to a cell proliferation promoting effect test and a collagen production effect test.
対照群には、抗老化用組成物が加えられない。 In the control group, no anti-aging composition is added.
細胞として、正常ヒト皮膚線維芽細胞を使用する。 Normal human skin fibroblasts are used as cells.
細胞増殖促進効果試験方法は以下のとおりである。 The cell proliferation promoting effect test method is as follows.
１）増殖した細胞をＦＢＳが含まれるダルベッコＭＥＭ培地に分散させ、９６ウェル培養プレートに接種する。続いて、細胞をＣＯ２インキュベータ（３７℃の条件で）に培養する。 1) The grown cells are dispersed in Dulbecco's MEM medium containing FBS and inoculated into a 96-well culture plate. Subsequently, the cells are cultured in a CO 2 incubator (at 37° C.).
２）接種した後の２日目に、培地を実施例１で得られる抗老化用組成物（質量パーセントでそれぞれ１２．５％、２５％、５０％、１００％加える）を添加した、ＦＢＳが含まれるダルベッコＭＥＭ培地及び対照群のＦＢＳが含まれるダルベッコＭＥＭ培地に交換し、続いてＣＯ２インキュベータで培養する。 2) On the second day after inoculation, the medium was prepared with FBS supplemented with the anti-aging composition obtained in Example 1 (adding 12.5%, 25%, 50%, and 100% by mass percentage, respectively). Change to Dulbecco's MEM medium containing and Dulbecco's MEM medium containing FBS of the control group, followed by culturing in a CO 2 incubator.
３）培養の最後の日に、プレート内の培地を除去し、ＦＢＳが含まれるダルベッコＭＥＭでＭＴＴ溶液を調製し、ＣＯ２インキュベータに入れて培養する。その後、抽出物及び微粒子を溶解し、スピードカウンターで吸光度を測定して細胞数を示すための値とする。測定結果を対照とし、１００として変換し、必要に応じてスチューデントのｔ検定で有意差検定を行い、結果を図１に示す。 3) On the last day of culture, remove the medium in the plate, prepare MTT solution with Dulbecco's MEM containing FBS, and culture in a CO2 incubator. Thereafter, the extract and microparticles are dissolved, and the absorbance is measured using a speed counter, which is used as a value to indicate the number of cells. The measurement results were used as a control and converted to 100, and if necessary, a significant difference test was performed using Student's t test, and the results are shown in FIG. 1.
図１と組み合わせると、２５％と５０％の含有量でヒト皮膚線維芽細胞の増加に対して明らかな促進作用を有する。しかし、１２．５％と１００％の含有量で、明らかに有意性がない。 In combination with FIG. 1, the contents of 25% and 50% have a clear promoting effect on the increase of human skin fibroblasts. However, there is clearly no significance at 12.5% and 100% contents.
コラーゲン生成促進試験方法は以下のとおりである。 The collagen production promotion test method is as follows.
１）増殖した細胞をＦＢＳが含まれるダルベッコＭＥＭ培地に分散させ、９６ウェル培養プレートに接種する。続いて、細胞をＣＯ２インキュベータに（３７℃の条件で）培養する。 1) The grown cells are dispersed in Dulbecco's MEM medium containing FBS and inoculated into a 96-well culture plate. Subsequently, the cells are cultured in a CO 2 incubator (at 37° C.).
２）接種後の２日目に、培地を実施例１で得られる抗老化用組成物（質量パーセントでそれぞれ１２．５％、２５％、５０％添加する）及び対照群のＦＢＳが含まれるダルベッコＭＥＭ培地が添加された培地に交換し、続いてＣＯ２インキュベータで培養する。 2) On the second day after inoculation, the culture medium was prepared using Dulbecco's anti-aging composition obtained in Example 1 (added at 12.5%, 25%, and 50% by mass percentage, respectively) and FBS as a control group. The medium is replaced with a medium supplemented with MEM medium, followed by culturing in a CO 2 incubator.
３）その後、上澄み液を収集し、ＥＬＩＳＡ法でＩ型コラーゲンの量を測定する。上澄み液における１単位当たりのタンパク質のＩ型コラーゲン量をそれぞれ収集し、溶解細胞により算出、推算して評価し、結果を図２に示す。 3) Then, collect the supernatant and measure the amount of type I collagen by ELISA method. The amount of type I collagen protein per unit in the supernatant was collected, calculated and estimated using lysed cells, and evaluated. The results are shown in FIG. 2.
図２と組み合わせると、抗老化用組成物を含有するアイクリームを培地に添加した場合、対照群と比較し、１単位当たりのタンパク質のＩ型コラーゲン量が増加し、２５％を添加した場合、有意差が観察される。 Combined with Figure 2, when the eye cream containing the anti-aging composition was added to the medium, the amount of type I collagen per unit of protein increased compared to the control group, and when 25% was added, A significant difference is observed.
以上より、本発明の抗老化用組成物は、正常ヒト皮膚線維芽細胞に対して増殖促進作用を有し、コラーゲン生成促進効果があり、Ｉ型コラーゲンが肌の弾性や強さに明らかに関連し、このため、本発明の抗老化用組成物を製品に添加して使用すれば、肌の弾性を改善し、シワを改善する効果がある。 From the above, the anti-aging composition of the present invention has a proliferation-promoting effect on normal human skin fibroblasts and a collagen production-promoting effect, and type I collagen is clearly related to skin elasticity and strength. Therefore, when the anti-aging composition of the present invention is added to a product, it has the effect of improving skin elasticity and reducing wrinkles.
上述した記載は、本発明の好適な実施例に過ぎず、任意の形態で本発明を実質的に制限するものではない。当業者が、本発明に記載の方法から逸脱することなく、さらに様々な改良と補足を行うことができ、これらの改良と補足が本発明の保護範囲と見なすべきであることに留意したい。当業者が、本発明の精神と範囲から逸脱することなく、以上に開示されている技術的内容を利用してなされたいくつかの変更、修飾と進化の等同変化は、いずれも本発明の等価実施例となり、また、本発明の実質的技術に基づいて上記実施例に対してなされたいかなる等同変化の変更、修飾及び進化も、依然として本発明の技術的解決手段の範囲内にあるものとする。 The above description is only a preferred embodiment of the invention and is not intended to substantially limit the invention in any way. It is noted that those skilled in the art can make various further improvements and supplements without departing from the method described in the present invention, and these improvements and supplements should be considered as the protection scope of the present invention. Any number of changes, modifications and evolutions that can be made by those skilled in the art using the technical content disclosed above without departing from the spirit and scope of the present invention are equivalents of the present invention. Examples, and any equivalent changes, modifications and evolutions made to the above embodiments based on the substantive technology of the present invention shall still fall within the scope of the technical solutions of the present invention. .
［付記］
［付記１］
ナノリポソームと、前記ナノリポソームにロードされる、ジグルコシル没食子酸、グルコシルヘスペリジン、キバナオランダセンニチエキス、ビオチン、及びカフェインを含む活性成分と、を含むことを特徴とする、抗老化用組成物。
[Additional notes]
[Additional note 1]
1. An anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, including diglucosyl gallic acid, glucosyl hesperidin, Lacustilla japonica extract, biotin, and caffeine.
［付記２］
重量パーセントで、０．０１１％－１．１％のジグルコシル没食子酸、０．０１％－１．５％のグルコシルヘスペリジン、０．０１％－０．８％のキバナオランダセンニチエキス、０．００００１％－０．０００５％のビオチン、０．００００１％－０．０００５％のカフェイン、０．６％－２．６％のコレステロール、０．１％－１．５％のベヘニルアルコール、６％－６．５％の水添レシチン、及び残部の脱イオン水を含むことを特徴とする、付記１に記載の抗老化用組成物。
[Additional note 2]
In weight percentages, 0.011%-1.1% diglucosyl gallic acid, 0.01%-1.5% glucosyl hesperidin, 0.01%-0.8% Lacustrum extract, 0.00001 %-0.0005% biotin, 0.00001%-0.0005% caffeine, 0.6%-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6 Anti-aging composition according to appendix 1, characterized in that it comprises .5% hydrogenated lecithin and the balance deionized water.
［付記３］
重量パーセントで、０．０１１％－０．５％のジグルコシル没食子酸、０．０１％－０．５％のグルコシルヘスペリジン、０．０４４７％－０．６％のキバナオランダセンニチエキス、０．００００１％－０．０００５％のビオチン、０．００００１％－０．０００５％のカフェイン、０．６％－２．６％のコレステロール、０．１％－１．５％のベヘニルアルコール、６％－６．５％の水添レシチン、及び残部の脱イオン水を含むことを特徴とする、付記１に記載の抗老化用組成物。
[Additional note 3]
In weight percentages, 0.011%-0.5% diglucosyl gallic acid, 0.01%-0.5% glucosyl hesperidin, 0.0447%-0.6% Laminaria japonica extract, 0.00001 %-0.0005% biotin, 0.00001%-0.0005% caffeine, 0.6%-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6 Anti-aging composition according to appendix 1, characterized in that it comprises .5% hydrogenated lecithin and the balance deionized water.
［付記４］
重量パーセントで、０．０１１％のジグルコシル没食子酸、０．５％のグルコシルヘスペリジン、０．０４４７％のキバナオランダセンニチエキス、０．０００１％のビオチン、０．０００１％のカフェイン、０．６％－２．６％のコレステロール、０．１％－１．５％のベヘニルアルコール、６％－６．５％の水添レシチン、及び残部の脱イオン水を含むことを特徴とする、付記１に記載の抗老化用組成物。
[Additional note 4]
In weight percentages, 0.011% diglucosyl gallic acid, 0.5% glucosyl hesperidin, 0.0447% Lacustnia chinensis extract, 0.0001% biotin, 0.0001% caffeine, 0.6 %-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the balance deionized water. Anti-aging composition as described.
［付記５］
前記ナノリポソームの粒径が１００－２５０ｎｍであることを特徴とする、付記１－４のいずれか１つに記載の抗老化用組成物。
[Additional note 5]
The anti-aging composition according to any one of appendices 1 to 4, wherein the nanoliposome has a particle size of 100 to 250 nm.
［付記６］
ａ）活性成分とナノリポソームを溶解し、混合溶液を得る工程と、
ｂ）工程ａ）の混合溶液中の溶媒を蒸発させて除去し、４０－６０℃で、脱イオン水を加え、１５－３５Ｈｚの超音波周波数で超音波処理を１５－２０分行い、超音波処理を完了した後、濾過して抗老化用組成物を得る工程と、を含むことを特徴とする、付記１－５のいずれか１つに記載の抗老化用組成物の製造方法。
[Additional note 6]
a) dissolving the active ingredient and nanoliposome to obtain a mixed solution;
b) Remove the solvent in the mixed solution of step a) by evaporation, add deionized water at 40-60 °C, and perform ultrasonic treatment for 15-20 minutes at an ultrasonic frequency of 15-35 Hz. After completing the treatment, the method for producing an anti-aging composition according to any one of Supplementary Notes 1 to 5, comprising the step of filtering to obtain an anti-aging composition.
［付記７］
付記１－５のいずれか１つに記載の抗老化用組成物の化粧品製造への応用であって、化粧品が、化粧水、フェイスパック、エッセンス、アイクリーム、アイパック、スキンケアゲル、スキンケア乳液、フェイスクリーム、又はスプレーを含むことを特徴とする、抗老化用組成物の応用。
[Additional note 7]
Application of the anti-aging composition according to any one of Supplementary Notes 1-5 to the production of cosmetics, wherein the cosmetics include lotions, face packs, essences, eye creams, eye packs, skin care gels, skin care emulsions, Application of an anti-aging composition, characterized in that it comprises a face cream or spray.
［付記８］
前記化粧品には、溶媒、増粘剤、防腐剤、ｐＨ調整剤、保湿剤、エモリエント剤、キレート剤、乳化剤、精油、香料、色素のうちの少なくとも１つを含む添加物がさらに含まれることを特徴とする、付記７に記載の抗老化用組成物の応用。
[Additional note 8]
The cosmetic product further includes an additive including at least one of a solvent, a thickener, a preservative, a pH adjuster, a humectant, an emollient, a chelating agent, an emulsifier, an essential oil, a fragrance, and a pigment. Application of the anti-aging composition according to appendix 7, characterized in that:
［付記９］
前記化粧品が、重量部で、抗老化用組成物１２．５－１００部、水添ナタネ油アルコール０．１－０．８部、リンゴ酸ジイソステアリル５－１０部、１，３－プロパンジオール５－１０部、カプリル酸ヤシ油アルキル３－１２部、マカデミアナッツ脂肪酸フィトステリル０．０１－０．１部、パルミチン酸レチノール０．０１－２部、ヘスペリジンメチルカルコン０．０１－０．５部及び脱イオン水５０－８０部を含むアイクリームであることを特徴とする、付記８に記載の抗老化用組成物の応用。
[Additional note 9]
The cosmetic product contains, in parts by weight, 12.5-100 parts of anti-aging composition, 0.1-0.8 parts of hydrogenated rapeseed oil alcohol, 5-10 parts of diisostearyl malate, and 1,3-propanediol. 5-10 parts of coconut oil alkyl caprylate, 3-12 parts of macadamia nut fatty acid phytosteryl, 0.01-0.1 part of retinol palmitate, 0.01-0.5 part of hesperidin methyl chalcone, and Application of the anti-aging composition according to appendix 8, characterized in that it is an eye cream containing 50-80 parts of ionized water.
［付記１０］
前記アイクリームの製造方法は、
１）水添ナタネ油アルコール、リンゴ酸ジイソステアリル、パルミチン酸レチノール、カプリル酸ヤシ油アルキル、マカデミアナッツ脂肪酸フィトステリル、及びヘスペリジンメチルカルコンを加えて均一に撹拌し、５０－９０℃に加熱して均一に撹拌し、油相とする工程と、
２）抗老化用組成物、水、及び１，３－プロパンジオールを均一に混合して撹拌し、水相とする工程と、
３）工程１）で得られた油相と工程２）で得られた水相を混合し、得られた混合物を超臨界装置の反応釜内に入れ、超臨界装置の反応釜内の温度が４５－６５℃になるように二酸化炭素を注入して調整し、圧力を１２－１５Ｍｐａに、時間を３０－４５分に設定し、減圧して二酸化炭素を放出し、室温まで冷却して、アイクリームを得る工程と、を含むことを特徴とする、付記９に記載の抗老化用組成物の応用。
[Additional note 10]
The method for producing the eye cream includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, coconut oil alkyl caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methylchalcone, stir evenly, and heat to 50-90°C to make a uniform mixture. Stirring to form an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction pot of the supercritical device, and raise the temperature inside the reaction pot of the supercritical device. Adjust the temperature by injecting carbon dioxide to 45-65℃, set the pressure to 12-15Mpa and time to 30-45 minutes, reduce the pressure to release carbon dioxide, cool to room temperature, Application of the anti-aging composition according to appendix 9, characterized in that it comprises the step of obtaining a cream.
Claims (10)
ｂ）工程ａ）の混合溶液中の溶媒を蒸発させて除去し、４０－６０℃で、脱イオン水を加え、１５－３５Ｈｚの超音波周波数で超音波処理を１５－２０分行い、超音波処理を完了した後、濾過して抗老化用組成物を得る工程と、を含むことを特徴とする、請求項１－５のいずれか１項に記載の抗老化用組成物の製造方法。 a) dissolving the active ingredient and nanoliposome to obtain a mixed solution;
b) Remove the solvent in the mixed solution of step a) by evaporation, add deionized water at 40-60 °C, and perform ultrasonic treatment for 15-20 minutes at an ultrasonic frequency of 15-35 Hz. The method for producing an anti-aging composition according to any one of claims 1 to 5, which comprises the step of filtering after completing the treatment to obtain an anti-aging composition.
１）水添ナタネ油アルコール、リンゴ酸ジイソステアリル、パルミチン酸レチノール、カプリル酸ヤシ油アルキル、マカデミアナッツ脂肪酸フィトステリル、及びヘスペリジンメチルカルコンを加えて均一に撹拌し、５０－９０℃に加熱して均一に撹拌し、油相とする工程と、
２）抗老化用組成物、水、及び１，３－プロパンジオールを均一に混合して撹拌し、水相とする工程と、
３）工程１）で得られた油相と工程２）で得られた水相を混合し、得られた混合物を超臨界装置の反応釜内に入れ、超臨界装置の反応釜内の温度が４５－６５℃になるように二酸化炭素を注入して調整し、圧力を１２－１５Ｍｐａに、時間を３０－４５分に設定し、減圧して二酸化炭素を放出し、室温まで冷却して、アイクリームを得る工程と、を含むことを特徴とする、請求項９に記載の抗老化用組成物の応用。 The method for producing the eye cream includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, coconut oil alkyl caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methylchalcone, stir evenly, and heat to 50-90°C to make a uniform mixture. Stirring to form an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction pot of the supercritical device, and raise the temperature inside the reaction pot of the supercritical device. Adjust the temperature by injecting carbon dioxide to 45-65℃, set the pressure to 12-15Mpa and time to 30-45 minutes, reduce the pressure to release carbon dioxide, cool to room temperature, Application of the anti-aging composition according to claim 9, characterized in that it comprises the step of obtaining a cream.
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