JP2023111692A - Mitophagy-enhancing agent - Google Patents

Abstract

To provide a novel composition that enhances mitophagy activity.SOLUTION: Provided is a mitophagy-enhancing agent that contains one or more selected from hypericum erectum extract, sage extract, pau d'arco extract, gardenia extract, Chinese tallow extract, soybean bud extract, pearl protein extract, white strawberry extract, royal jelly fermentation liquid, Salicornia europaea extract, daylily flower fermentation liquid, and chamomilla extract.SELECTED DRAWING: None

Description

TECHNICAL FIELD The present invention relates to compositions for enhancing the activity of mitophagy, mitochondria-selective autophagy.

In cells, there is a mechanism for degrading intracellular proteins, which is called autophagy. Autophagy maintains the homeostasis of living organisms by degrading abnormal or excessively synthesized proteins.

Mitophagy is a selective mitochondrial degradation mechanism mediated by autophagy and is believed to be involved in mitochondrial metabolism.
Damaged mitochondria produce active oxygen, which promotes oxidation of cells, but it is expected to have the effect of suppressing this by enhancing the activity of mitophagy.

For example, Patent Literature 1 describes that an extract of lotus flowers belonging to the genus Lotus of the family Lotus family has an effect of enhancing the activity of mitophagy.

JP 2017-178920 A

An object of the present invention is to provide a novel composition that enhances mitophagy activity.

The present invention for solving the above problems is Hypericum extract, sage extract, Pau d'Arco extract, gardenia extract, Scutellaria root extract, soybean bud extract, pearl protein extract, white strawberry extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid, and A mitophagy activity enhancer containing one or more extracts selected from chamomile extracts.
In a preferred embodiment of the present invention, the mitophagy activity enhancer includes at least a Hypericum perforatum extract.
According to the present invention, mitophagy activity in cells can be enhanced.

In a preferred embodiment of the present invention, the agent for enhancing mitophagy activity is for enhancing mitophagy activity in human skin fibroblasts and/or human epidermal keratinocytes.
According to the present invention, by enhancing the mitophagy activity in human skin fibroblasts and/or human epidermal keratinocytes, mitochondria, in particular, the prevention or improvement of aging conditions and diseases caused by damaged mitochondria. can be expected to

In a preferred embodiment of the present invention, the mitophagy activity enhancer is a cosmetic for enhancing mitophagy activity.

According to the present invention, a novel composition that enhances mitophagy activity can be provided.

In the examples, mitophagy activity in human skin fibroblasts and human epidermal keratinocytes cultured in an extract-free medium (negative control), and human skin fibroblasts and human epidermis cultured in a medium supplemented with Hypericum perforatum extract FIG. 4 is a diagram representing mitophagy activity in keratinocytes. In the examples, mitophagy activity in human skin fibroblasts and human epidermal keratinocytes cultured in an extract-free medium (negative control), and human skin fibroblasts and human epidermis cultured in a medium supplemented with sage extract FIG. 4 is a diagram representing mitophagy activity in keratinocytes. Fig. 2 is a diagram showing mitophagy activity in human epidermal keratinocytes cultured in an extract-free medium (negative control) and mitophagy activity in human epidermal keratinocytes cultured in a medium supplemented with a Pau d'Arco extract in Examples. be. Fig. 2 is a diagram showing mitophagy activity in human epidermal keratinocytes cultured in a medium containing no extract (negative control) and mitophagy activity in human epidermal keratinocytes cultured in a medium containing gardenia extract in Examples. . In the examples, mitophagy activity in human dermal fibroblasts and human epidermal keratinocytes cultured in an extract-free medium (negative control), and human dermal fibroblasts and human epidermis cultured in a medium supplemented with Scutellaria root extract FIG. 4 is a diagram representing mitophagy activity in keratinocytes. In the examples, mitophagy activity in human skin fibroblasts and human epidermal keratinocytes cultured in a medium containing no extract (negative control), and human skin fibroblasts cultured in a medium supplemented with soybean sprout extract and human FIG. 4 is a diagram showing mitophagy activity in epidermal keratinocytes. Fig. 4 is a diagram showing mitophagy activity in human epidermal keratinocytes cultured in an extract-free medium (negative control) and mitophagy activity in human epidermal keratinocytes cultured in a medium supplemented with pearl protein extract in Examples. be. In the examples, mitophagy activity in human skin fibroblasts and human epidermal keratinocytes cultured in an extract-free medium (negative control) and human skin fibroblasts cultured in a medium supplemented with white strawberry extract and human FIG. 3 is a diagram showing mitophagy activity in epidermal keratinocytes. Fig. 2 is a diagram showing mitophagy activity in human epidermal keratinocytes cultured in an extract-free medium (negative control) and mitophagy activity in human epidermal keratinocytes cultured in a medium supplemented with royal jelly fermentation liquid in Examples. be. Fig. 2 is a diagram showing mitophagy activity in human epidermal keratinocytes cultured in a medium containing no extract (negative control) and mitophagy activity in human epidermal keratinocytes cultured in a medium supplemented with Salicornia extract in Examples. . Fig. 3 shows the mitophagy activity in human epidermal keratinocytes cultured in an extract-free medium (negative control) and the mitophagy activity in human epidermal keratinocytes cultured in a medium supplemented with daily lily flower fermented liquid in Examples. is. Fig. 2 is a diagram showing mitophagy activity in human epidermal keratinocytes cultured in a medium containing no extract (negative control) and mitophagy activity in human epidermal keratinocytes cultured in a medium supplemented with chamomile extract in Examples. .

The mitophagy activity enhancer according to the present invention includes hypericum extract, sage extract, pau d'arco extract, gardenia extract, scutellaria root extract, soybean bud extract, pearl protein extract, white strawberry extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid. , and chamomile extract.
A preferred embodiment of the present invention contains at least a Hypericum perforatum extract.
According to the present invention, mitophagy activity in cells can be enhanced.

Mitochondria produce active oxygen with the production of ATP, but damaged mitochondria produce more active oxygen than normal (undamaged) mitochondria. Active oxygen is known to cause lifestyle-related diseases such as stroke and skin aging.
The present invention promotes degradation of damaged mitochondria by enhancing mitophagy activity in cells. This is expected to prevent diseases and aging caused by active oxygen produced by damaged mitochondria.

Hypericum extract, sage extract, Pau d'Arco extract, gardenia extract, Scutellaria root extract, soybean bud extract, pearl protein extract, white strawberry extract, Salicornia extract, and chamomile extract used in the present invention are Hypericum erectum, Sage (Salvia), officinalis), Pau d'arco (Handroanthus impetiginosus), gardenia (Gardenia jasminoides), soybean (Glycine max), pearl, white strawberry (Fragaria x ananassa), Salicornia europaea), or chamomile (Matricaria chamomilla) from the extract itself First, it means a general term for these extract fractions, purified fractions, extracts or fractions, and solvent-removed purified products. In addition, extracts derived from hypericum, sage, pau d'arco, gardenia, soybean, pearl, white strawberry, Salicornia and chamomile are extracts from wild or grown plants, extracts from those sold as raw materials for Chinese herbal medicines, etc., and commercially available extracts. and extracts containing
When the above extract is extracted from a plant, the extraction operation uses the whole plant part, as well as parts such as the plant body, the ground part, the rhizome part, the tree trunk, the leaf part, the stem part, the flower spike, and the flower bud. However, it is preferable to pulverize or finely chop these in advance to improve the extraction efficiency. Extraction solvents include water, alcohols such as ethanol, isopropyl alcohol and butanol, polyhydric alcohols such as 1,3-butanediol and polypropylene glycol, ketones such as acetone and methyl ethyl ketone, and ethers such as diethyl ether and tetrahydrofuran. One or more selected from polar solvents such as polar solvents can be exemplified as suitable. As the extraction solvent, it is preferable to employ water, alcohols, or a mixture of water and alcohols.

As a specific method for extracting the extract, for example, 1 to 30 parts by mass of a solvent is added to 1 mass of the part used for extraction of the plant body or the like or the dried product thereof, and the mixture is kept at room temperature for several days, near the boiling point. If it is at a temperature of , it is immersed for several hours, cooled to room temperature, then optionally removed insoluble matter and / or solvent, and fractionated and purified by column chromatography or the like, but the extraction method is not limited to this.

As the royal jelly fermented liquid, one obtained by fermenting commercially available royal jelly by a known method may be used, or a commercially available royal jelly fermented liquid may be used.
The day lily flower fermented liquid is obtained by fermenting wild or grown day lily flowers (preferably pre-crushed or finely chopped) with yeast, and a commercially available day lily flower fermented liquid can also be used. can.

The mitophagy activity enhancer of the present invention can take any dosage form by appropriately combining with any ingredient used for formulation. can also
A preferred form of the present invention is an external preparation for skin.
Oral preparations or skin preparations for external use can be manufactured by appropriately using known methods and devices.

When the mitophagy activity enhancer of the present invention is used as an oral preparation, Hypericum extract, Sage extract, Pau d'Arcosexes, Gardenia extract, Scutellaria root extract, Soybean bud extract, Pearl protein extract, White strawberry extract, Royal jelly fermented liquid, Salicornia extract, Day lily It is preferably in the form of a food composition containing one or more extracts selected from flower fermented liquid and chamomile extract as active ingredients. Specifically, it is preferably in the form of supplements having dosage forms such as general foods, tablets, granules, and drinks.

The content of the extract in the oral formulation is 0.1 mg or more, preferably 1 mg or more, and more preferably 10 mg or more in terms of the dry mass of the extract per intake, depending on the dosage form. is.
Also, it is usually 2000 mg or less, preferably 1000 mg or less, more preferably 500 mg or less.
Two types selected from Hypericum extract, sage extract, Pau d'Arco extract, gardenia extract, Scutellaria root extract, soybean bud extract, pearl protein extract, white strawberry extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid, and chamomile extract. In the case of an oral preparation containing the above extracts, the total content of each extract should be within the above numerical range.

When the agent for enhancing mitophagy activity of the present invention is used as an external preparation for skin, it can be exemplified in the form of cosmetics, quasi-drugs, external medicine for skin, and the like. Moreover, although the dosage form thereof is not particularly limited, it is more preferable to use it as a cosmetic.
Cosmetics are preferably in the form of facial cleansers, cleansing agents, lotions, serums, milky lotions, creams, gels, sun care products, and the like. It can also be in the form of a face pack.
When the mitophagy activity enhancer of the present invention is in the form of a skin external preparation, it may be in a form that is not removed after application, or in a form that is removed by wiping or washing after a certain period of time (for example, 5 to 10 minutes) after application. good too.

The content (dry weight) of the extract in the external preparation for skin is 0.00001% by mass or more, preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, and still more preferably. is 0.01% by mass or more, and more preferably 0.1% by mass or more.
Also, it is usually 80% by mass or less, preferably 30% by mass or less, more preferably 10% by mass or less.
Within the above range, mitophagy activity in cells can be enhanced.
Two types selected from Hypericum extract, sage extract, Pau d'Arco extract, gardenia extract, Scutellaria root extract, soybean bud extract, pearl protein extract, white strawberry extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid, and chamomile extract. In the case of an external preparation for skin containing the above extracts, the total content of each extract should be within the above numerical range.

When blending the above extracts in cosmetics, the following ingredients should be used within the range that does not impair the effect of enhancing mitophagy activity: whitening ingredients, wrinkle-improving ingredients, anti-inflammatory ingredients, hypericum extract, sage extract, pau d'arco extract, gardenia extract, Scutellaria root extract, soybean. Bud extract, pearl protein extract, white strawberry extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid, and animal and plant-derived extracts other than chamomile extract. It may be blended as
These optional components may be commercially available and blended, or those synthesized by known methods may be blended. Moreover, each optional component may have two or more effects (for example, a whitening effect and a wrinkle-improving effect).

As the whitening ingredient, ingredients generally used in cosmetics can be used without particular limitation.
For example, 4-n-butylresorcinol, ascorbic acid glucoside, 3-O-ethylascorbic acid, tranexamic acid, arbutin, 1-triphenylmethylpiperidine, 1-triphenylmethylpyrrolidine, 2-(triphenylmethyloxy)ethanol, 2-(triphenylmethylamino)ethanol, 2-(triphenylmethyloxy)ethylamine, triphenylmethylamine, triphenylmethanol, triphenylmethane and aminodiphenylmethane, N-(o-toluoyl)cysteic acid, N-(m -toluoyl)cysteic acid, N-(p-toluyl)cysteic acid, N-(p-methoxybenzoyl)cysteic acid, N-benzoyl-serine, N-(p-methylbenzoyl)serine, N-(p-ethylbenzoyl) ) serine, N-(p-methoxybenzoyl)serine, N-(p-fluorobenzoyl)serine, N-(p-trifluoromethylbenzoyl)serine, N-(2-naphthoyl)serine, N-(4-phenyl benzoyl)serine, N-(p-methylbenzoyl)serine methyl ester, N-(p-methylbenzoyl)serine ethyl ester, N-(2-naphthoyl)serine methyl ester, N-benzoyl-O-methylserine, N-( p-methylbenzoyl)-O-methylserine, N-(p-methylbenzoyl)-O-acetylserine, N-(2-naphthoyl)-O-methylserine, dexpanthenol W, niacinamide and the like.

The content of whitening ingredients in cosmetics is usually 0.0001 to 30% by mass, preferably 0.001 to 10% by mass, more preferably 0.01 to 5% by mass (dry mass in the case of extracts). .

As the wrinkle-improving ingredient, ingredients generally used in cosmetics can be used without particular limitation.
For example, vitamin A or derivatives thereof include retinol, retinal, retinoic acid, tretinoin, isotretinoin, tocopherol retinoate, retinol palmitate, and retinol acetate. Further, benzyl ursolic acid ester, ursolic acid phosphate, betulinic acid benzyl ester, benzilic acid phosphate, trifluoride isopropyloxopropylaminocarbonylpyrrolidinecarbonylmethylpropylaminocarbonylbenzoylamino sodium acetate, niacinamide and the like can be mentioned.

The content of the wrinkle-improving component in the cosmetic is usually 0.0001 to 30% by mass, preferably 0.001 to 10% by mass, more preferably 0.01 to 5% by mass (in the case of an extract, the dry mass ).

Examples of anti-inflammatory components include clarinone, glabridin, glycyrrhizic acid, glycyrrhetinic acid, pantothenyl alcohol, niacinamide, tranexamic acid, glycyrrhizic acid and its salts, alkyl glycyrrhetinate and its salts, and glycyrrhetinic acid and Its salts are preferred.
The content of the anti-inflammatory ingredient in the cosmetic is usually 0.01 to 30% by mass, preferably 0.1 to 10% by mass, more preferably 1 to 5% by mass (dry mass in the case of an extract). .

Examples of extracts derived from animals and plants include akebi extract, asunaro extract, asparagus extract, avocado extract, amacha extract, almond extract, arnica extract, aronia extract, apricot extract, ginkgo biloba extract, fennel extract, udo extract, Eleuthero extract, Meiso extract, Phellodendron bark extract, Panax ginseng extract, Dead nettle extract, Kayoku extract, Kakon extract, Carrot extract, Artemisia versicolor extract, Glycyrrhiza extract, Kiwi extract, Cucumber extract, Guava extract, Kumazasa extract, Walnut extract, Black rice extract, Chlorella extract, Mulberry extract, Astragalus extract, Alpinia extract, Gentian extract, Rice extract, Fermented rice extract, Fermented rice bran extract, Rice germ oil, Salvia extract, Saponaria extract, Sasa extract, Sansha extract, Sansho extract, Shiitake extract, Rhododendron extract, Chikon extract, Perilla extract, linden extract, meadowsweet extract, ginger extract, calamus root extract, horsetail extract, stevia extract, fermented stevia extract, yarrow extract, mint extract, mallow extract, neem extract, jersey extract, sahakuhi extract, rhubarb extract, soybean extract, taisou extract, dandelion extract, clove extract, capsicum extract, angelica extract, calendula officinalis extract, tonin extract, dokudami extract, tomato extract, natto extract, carrot extract, garlic extract, hibiscus extract, Bakumondou extract, lotus extract, parsley extract, birch extract , hamamelis extract, cypress extract, cypress extract, loquat extract, coltsfoot extract, coltsfoot extract, bukuryo extract, luffa extract, peppermint extract, bodaiju extract, pine extract, skunk cabbage extract, melissa extract, mozuku extract, peach extract, cornflower extract, lily extract, coix extract , mugwort extract, lavender extract, apple extract, rooibos tea extract, litchi extract, lettuce extract, forsythia extract, astragalus extract, rosemary extract, burnet extract, and the like.

The content (dry mass) of any animal or plant-derived extract in the cosmetic is usually 0.01 to 30% by mass, preferably 0.1 to 10% by mass, more preferably 0.3 to 3% by mass. preferable.

Ingredients commonly used in cosmetics other than active ingredients include polyethylene glycol, glycerin, 1,3-butylene glycol, erythritol, sorbitol, xylitol, lutitol, propylene glycol, dipropylene glycol, diglycerin, isoprene glycol, 1, Polyols such as 2-pentanediol, 2,4-hexylene glycol, 1,2-hexanediol, 1,2-octanediol, fatty acid soaps (sodium laurate, sodium palmitate, etc.), potassium lauryl sulfate, trialkyl sulfates Anionic surfactants such as ethanolamine ether, cationic surfactants such as stearyltrimethylammonium chloride, benzalkonium chloride, laurylamine oxide, imidazoline amphoteric surfactants (2-cocoyl-2-imidazolinium hydroxide -1-carboxyethyloxy disodium salt, etc.), betaine surfactants (alkylbetaine, amidobetaine, sulfobetaine, etc.), amphoteric surfactants such as acylmethyl taurine, sorbitan fatty acid esters (sorbitan monostearate, sorbitan sesquioleate, etc.), glycerin fatty acids (glyceryl monostearate, etc.), propylene glycol fatty acid esters (propylene glycol monostearate, etc.), hydrogenated castor oil derivatives, glycerin alkyl ethers, POE sorbitan fatty acid esters (POE sorbitan monostearate, etc.) oleate, polyoxyethylene sorbitan monostearate, etc.), POE sorbitol fatty acid esters (POE-sorbitol monolaurate, etc.), POE glycerin fatty acid esters (POE-glycerin monoisostearate, etc.), POE fatty acid esters (polyethylene glycol monooleate, POE distearate, etc.), POE alkyl ethers (POE 2-octyldodecyl ether, etc.), POE alkylphenyl ethers (POE nonylphenyl ether, etc.), Pluronic (registered trademark) types, POE/POP alkyl ethers ( POE/POP 2-decyltetradecyl ether, etc.), tetronics, POE castor oil/hydrogenated castor oil derivatives (POE castor oil, POE hydrogenated castor oil, etc.), sucrose fatty acid esters, nonionic surfactants such as alkyl glucosides , Moisturizing ingredients such as sodium pyrrolidonecarboxylate, lactic acid, sodium lactate, surface-treated mica, talc, kaolin, synthetic mica, calcium carbonate, magnesium carbonate, anhydrous silicic acid (silica), aluminum oxide, sulfuric acid Powders such as barium, optionally surface-treated inorganic pigments such as cobalt oxide, ultramarine blue, Prussian blue, zinc oxide, optionally surface-treated composite pigments such as iron oxide titanium dioxide sintered body, surface Pearl agents such as titanium mica, fish phosphorus foil, and bismuth oxychloride, which may be treated, Red No. 202, Red No. 228, Red No. 226, Yellow No. 4, Blue No. 404, which may be laked, Yellow No. 5, Red No. 505, Red No. 230, Red No. 223, Orange No. 201, Red No. 213, Yellow No. 204, Yellow No. 203, Blue No. 1, Green No. 201, Purple No. 201, Red No. 204, etc. Pigments, polyethylene powder, polymethyl methacrylate, nylon powder, organic powders such as organopolysiloxane elastomer, lower alcohols such as ethanol and isopropanol, vitamin A or its derivatives, vitamin B6 hydrochloride, vitamin B6 tripalmitate , vitamin B6 dioctanoate, vitamin B2 or its derivatives, vitamin B12, vitamin B15 or its derivatives such as vitamin B, α-tocopherol, β-tocopherol, γ-tocopherol, vitamin E such as vitamin E acetate, vitamin D, Examples include vitamins such as vitamin H, pantothenic acid, pantethine, and pyrroloquinoline quinone.

In a preferred embodiment of the present invention, the agent for enhancing mitophagy activity is a composition for enhancing mitophagy activity in human skin fibroblasts and/or human epidermal keratinocytes.
In a more preferred embodiment of the present invention, the agent for enhancing mitophagy activity is a composition for enhancing mitophagy activity in human skin fibroblasts and human epidermal keratinocytes.
Human dermal fibroblasts tend to express the aging state of the skin (for example, spots, wrinkles, sagging, etc.) due to aging. In addition, active oxygen is known to be one of the causes of the appearance of skin aging due to aging in human skin fibroblasts. According to the present invention, the mitophagy activity in human skin fibroblasts is enhanced, and the degradation of damaged mitochondria is promoted. As a result, the production of active oxygen by damaged mitochondria is suppressed, so the present invention is expected to be able to suppress and/or improve the aging state of the skin caused by aging.
In addition, human epidermal keratinocytes are more likely to experience a milder aging state than the above-mentioned aging states that appear with aging in human skin fibroblasts, such as dryness and disordered turnover. Active oxygen is known to be one of the causes of the appearance of these mild aging states. According to the present invention, mitophagy activity in human epidermal keratinocytes is enhanced, and degradation of damaged mitochondria is promoted. Since this suppresses the production of active oxygen, the present invention is expected to be able to suppress and/or improve the occurrence of the mild aging state.

EXAMPLES The present invention will be described in more detail below with reference to examples, but the technical scope of the present invention is not limited to the following examples.

Using a mitophagy detection kit (Doujin Kagaku), mitophagy in human epidermal keratinocytes (NHEK) and/or human dermal fibroblasts (NHDF) cultured in a medium supplemented with each extract and a medium without extracts was detected. Detected.

<Test method>
(1) Human epidermal keratinocytes and human skin fibroblasts were seeded in a medium suitable for each cell at 4×10 ⁴ cells/well and cultured at 37° C. for 24 hours.
(2) Each sample was washed twice with 500 uL/well of PBS(-) after removing the medium.
(3) 150 uL/well of 100 nM Mtphagy Dye working solution prepared according to the kit was added and incubated at 37° C. for 30 minutes.
(4) After removing the supernatant, the cells were washed twice with 500 uL/well of PBS(-).
(5) Hypericum extract, sage extract, Pau d'Arco extract, gardenia extract, Scutellaria root extract, soybean bud extract, pearl protein extract, white strawberry extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid, or chamomile extract at final concentration It was added to a medium suitable for each cell so as to make 0.1%, and the medium containing each extract was added to the cells washed in (4) above. A medium containing no extract was added to the cells washed in (4) above as a negative control. Each cell was cultured at 37° C. for 24 hours.
(6) In order to confirm the co-localization of the Mtphagy Dye added in (3) above and the lysosome, 1 uM Lyso Dye DMSO stock solution prepared according to the kit was added to each sample at 150 µL/well and incubated at 37°C for 30 minutes. .
(7) After removing the supernatant, wash once with PBS (-) 500 uL / well, add an equal amount of PBS (-) again, and check the degree of staining of each cell with a fluorescence microscope (KEYENCE BZ-X800). Observation was made at a magnification of 10×20 (objective lens).

The observation results are shown in FIGS. 1 to 12. FIG.
Visually check the degree of staining and compare the fluorescence intensity of each cell cultured in the medium without the extract (negative control). , evaluated that the mitophagy activity in the cells was enhanced.

As shown in FIGS. 1, 2, 5, 6, 7 and 8, cells cultured in medium supplemented with Hypericum perforatum extract, sage extract, Scutellaria root extract, soybean sprout extract, or white strawberry extract Fluorescence intensity was increased compared to cells cultured in supplemented medium (negative control). That is, it is suggested that mitophagy activity was enhanced in human skin fibroblasts and human epidermal keratinocytes cultured in a medium supplemented with Hypericum perforatum extract, sage extract, Scutellaria root extract, soybean sprout extract, or white strawberry extract. Ta.

3, 4, 7, 9, 10, 11 and 12, Pau d'Arco extract, gardenia extract, pearl protein extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid, or The human epidermal keratinocytes cultured in the chamomile extract-added medium had a higher fluorescence intensity than the human epidermal keratinocytes cultured in the extract-free medium (negative control). Specifically, mitophagy activity was enhanced in human epidermal keratinocytes cultured in a medium supplemented with Pau d'Arco extract, gardenia extract, pearl protein extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid, or chamomile extract. was suggested.

Also, as described above, damaged mitochondria produce active oxygen, and this active oxygen causes the appearance of skin aging due to aging in human skin fibroblasts (e.g., spots, wrinkles, sagging, etc.). It is known to be one of the causes of the appearance of mild aging (dryness, disordered turnover, etc.) in epidermal keratinocytes.
Therefore, the composition containing at least one of the above extracts suppresses the production of reactive oxygen by damaged mitochondria by enhancing mitophagy activity in human skin fibroblasts and/or human skin epidermal keratinocytes, Therefore, it can be expected that the appearance of aging and/or mild aging of the skin can be suppressed and/or prevented. In other words, it was suggested that the mitophagy activity enhancer according to the present invention can be used as an agent (including cosmetics) for suppressing and/or preventing the appearance of aging and/or mild aging. .

Production examples of the mitophagy activity enhancer according to the present invention are shown below. The unit is % by mass.

<Manufacturing example>
1. Preparation of each phase (A) phase: After dissolving ethanol in water, add Hypericum perforatum extract to prepare a uniform solution.
(B) Phase: Heated to 80° C. or higher to dissolve uniformly.
(C) Phase: Xanthan gum is dispersed in BG.
(D) Phase: After dissolving glycerin in water, dissolve citric acid and Na citrate to make a pH buffer solution.
2. Preparation method (1) Heated (B) is added to (A), stirred for several minutes, and then cooled to 30°C.
(2) Mix (D) with (C) while stirring, and stir until uniformly dissolved.
(3) Slowly pour the mixed phase of (A) and (B) into the mixed phase of (C) and (D).

ADVANTAGE OF THE INVENTION According to this invention, the composition which can enhance the mitophagy activity in a cell can be provided.

Claims (4)

One or more extracts selected from Hypericum extract, sage extract, Pau d'Arco extract, gardenia extract, Scutellaria root extract, soybean bud extract, pearl protein extract, white strawberry extract, royal jelly fermented liquid, Salicornia extract, day lily flower fermented liquid, and chamomile extract A mitophagy activity enhancer comprising. The mitophagy activity enhancer according to claim 1, comprising at least a Hypericum perforatum extract. The mitophagy activity enhancer according to claim 1 or 2, which enhances mitophagy activity in human skin fibroblasts and/or human epidermal keratinocytes. The mitophagy activity enhancer according to any one of claims 1 to 3, which is a cosmetic.