JP2023108695A - Topical skin preparation - Google Patents

Abstract

To provide a topical skin preparation that is made from a natural product and has excellent biological safety and comprises a novel active ingredient to give moisture to skin and protect it from drying.SOLUTION: A topical skin preparation comprises, as an active ingredient, a mixture of fermented seeds of Coix plants, extract from Salicornia plants and extract from Aspalathus plants.SELECTED DRAWING: Figure 1

Description

TECHNICAL FIELD The present invention relates to an external preparation for skin that increases amino acids in the stratum corneum and exerts a skin moisturizing function.

Conventionally, it is known that the natural moisturizing factor (NMF) of the skin decreases due to factors such as aging, resulting in a decrease in moisture and dryness of the skin. Conventionally, various moisturizing agents (glycerin, polyhydric alcohol, sodium lactate, etc.) have been proposed to improve skin moisture and dryness, but in recent years, they are derived from natural products and have high safety and effectiveness. components with high

In view of the problems of the prior art, the present inventors conducted extensive research to discover new active ingredients derived from natural products from the viewpoint of skin safety. As a result, the active ingredient combining the fermented Coix seeds, the Salicornia extract and the Aspalathus linearis extract exhibits a remarkable effect of promoting the gene expression of the natural moisturizing factor (NMF)-related protein, moisturizes the skin, It has been found to prevent and improve dryness.

Conventionally, it has been disclosed, for example, in Patent Documents 1 to 3 that each of the fermented Coix seeds, Salicornia extract and Aspalathus linearis extract has skin physiological activity. It was not known that the combined active ingredient exerts a remarkable effect of promoting gene expression of natural moisturizing factor (NMF)-related protein.
JP-A-2005-145878 Japanese Patent Application Laid-Open No. 2010-138139 Japanese Patent Application Laid-Open No. 2009-249371

The present invention is a mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract (solid concentration ratio of fermented Coix seed: Salicornia extract: Aspalathus linearis extract = 5.5 to 6.0 :3.0-3.5:1) as an active ingredient.
In addition, the present invention provides a mixture of a fermented product of Coix seeds, a Salicornia extract and an extract of Aspalathus linearis (the solid concentration ratio is fermented product of Coix seeds: Salicornia extract: Aspalathus linearis extract = 5.5). ~6.0:3.0~3.5:1) is a peptidylarginine deiminase-1 (PADI-1) gene expression promoter containing as an active ingredient.
In addition, the present invention provides a mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract (solid concentration ratio of fermented Coix seed: Salicornia extract: Aspalathus linearis extract = 5.5 to 6 0:3.0 to 3.5:1) as an active ingredient.
In addition, the present invention provides any of the filaggrin gene expression promoter, peptidylarginine deiminase-1 (PADI-1) gene expression promoter and peptidylarginine deiminase-3 (PADI-3) gene expression promoter. It is a skin preparation for external use.

The present invention uses a mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract as active ingredients to moisturize the skin based on the effect of promoting gene expression of natural moisturizing factor (NMF)-related proteins. , it is possible to provide an external preparation for skin that prevents and improves dryness.

FIG. 2 is a diagram showing the filaggrin gene expression promoting effect of the active ingredient according to the present invention. FIG. 2 is a diagram showing the peptidylarginine deiminase-1 (PADI-1) gene expression promoting effect of the active ingredient according to the present invention. FIG. 2 is a diagram showing peptidylarginine deiminase-3 (PADI-3) gene expression promoting effects of active ingredients according to the present invention.

In the present invention, "coix seed" refers to a plant belonging to the genus Juzdama of the Poaceae family, and may be of any species. As the part used for fermentation, seeds are preferable, and both those with shells and those without shells can be used. , high-temperature and high-pressure processed powders, and the like.

Yeast is preferably used as the fungus used for the fermentation of adlay. Preferred yeasts are those belonging to the genus Saccharomyces, such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus.

Salicornia used in the present invention is a plant belonging to the genus Salicornia in the Chenopodiaceae family, and is sometimes called coral grass. Examples include Salicornia europaea, Salicornia europaea, Salicornia bigelovi, Salicornia dolicostachia Ssp strictissima and the like. Moreover, the whole plant is preferable as the part to be used for extraction.

Aspalathus linearis used as a material for the Aspalathus linearis extract used in the present invention is a plant belonging to the genus Aspalathus of the Fabaceae family, and is unfermented and green (so-called green rooibos). preferably used. Moreover, the whole plant or leaves are preferable as the site to be used for extraction.

For the preparation of the extract, first, the part of each plant to be used is washed with water in advance to remove foreign substances, if necessary, then dried as it is or after being dried, cut into small pieces or pulverized as necessary, and brought into contact with an extraction solvent. Extract. Extraction can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method.

Examples of extraction solvents include water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; Used.

Among the above-mentioned extraction solvents, from the viewpoint of the effectiveness of the mixture of extracts obtained, and further from the viewpoint of skin irritation, it can be widely applied to external skin preparations (cosmetics, quasi-drugs, etc.). Also from this point of view, in the present invention, hydrophilic solvents such as water, lower alcohols or polyhydric alcohols are suitable. Preferable examples when using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or water and lower alcohols (especially ethanol), or the use of a mixed solvent of water and polyhydric alcohols (especially 1,3-butylene glycol, glycerin), etc. Among them, water alone, or water and 1,3 - Mixed solvents of butylene glycol are particularly preferred.

When a mixed solvent is used, the mixing ratio is, for example, in the case of a mixed solvent of water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1:1 to 20:1, and in the case of a mixed solvent of water and ethanol. If so, it is preferably in the range of 1:1 to 25:1, and if it is a mixed solvent of water and glycerin, it is preferably in the range of 1:1 to 20:1.

In addition, the weight ratio of the used part of each plant to the extraction solvent is preferably 1:1 to 1:50, more preferably 1:2 to 1:30.

When preparing the extract solution, the pH is not particularly limited, but is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, and the desired may be adjusted to a pH of

Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. , the extraction temperature is preferably in the range of 0°C to 80°C, more preferably in the range of 0°C to 20°C, and the extraction time is preferably 1 to 168 hours (1 hour to 1 week). , more preferably in the range of 1 to 120 hours (1 hour to 5 days).

The extract solution prepared as described above may be mixed, generally adjusted to pH 3 to 8, and used as it is as a compounding agent for an external skin preparation, or may be concentrated under reduced pressure. Any desired concentration may be used. Also, the extract may be dried by a conventional method such as a spray drying method.

The solid content concentration of the fermented pearl barley solution according to the present invention is preferably 0.2% by weight to 5.0% by weight, more preferably 0.3% by weight to 1.5% by weight. The solid content concentration of the Salicornia extract solution according to the present invention is preferably 0.2 to 5.0% by weight, more preferably 1.0% to 3.0% by weight in the extract. The solid content concentration of the Aspalathus linearis extract solution is preferably 0.2 wt % to 5.0 wt %, more preferably 0.8 wt % to 2.0 wt %, based on the extract.

The Coix fermented product solution, the Salicornia extract solution, and the Aspalathus linearis extract solution prepared as described above are mixed to prepare a composition (the mixed solution is hereinafter referred to as the "composition"). The solid content concentration of the entire composition is preferably 0.8% by weight to 3.0% by weight, more preferably 0.4% by weight to 2.0% by weight. In addition, the solid concentration ratio of the fermented Coix seed solution, the Salicornia extract solution, and the Aspalathus linearis extract solution in the composition is from 5.5 to 5.5 from the viewpoint of promoting the expression of genes of moisturizing-related proteins shown below. 6.0:3.0 to 3.5:1 are preferred.

When the composition of the present invention is incorporated into an external preparation for skin, it is preferably incorporated in an amount of 0.01 to 10% by weight or more as a solid content concentration of the composition.

Skin external preparations (cosmetics, quasi-drugs, etc.) containing the composition of the present invention include, for example, emulsions, creams, lotions, essences, transparent gels, sunscreens, packs, lipsticks, foundations, sheet masks, and liquids. Cleansing cosmetics such as foundation, makeup press powder, blusher, white powder, makeup remover, facial cleanser, body shampoo, hair shampoo, soap, and the like.

When the composition according to the present invention is blended into external skin preparations (cosmetics, quasi-drugs, etc.), components used in external skin preparations, such as oily components, surfactants (synthetic, natural products), Moisturizers, thickeners, emulsifiers or emulsifiers, preservatives/bactericides, powder ingredients, UV absorbers, antioxidants, pigments, fragrances, anti-wrinkle agents, other bioactive ingredients, etc. can be compounded. In addition, as long as the effectiveness and features of the composition according to the present invention are not impaired, it may be combined with other physiologically active ingredients and blended into external preparations for skin.

Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadowfoam oil, shea butter, tea tree oil, and avocado oil. , macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil and other plant-derived squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; beeswax, Waxes such as carnauba wax, rice wax and lanolin; Hydrocarbons such as liquid paraffin, petrolatum, paraffin wax and squalane; Fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and cis-11-eicosenoic acid. Higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthesis of isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexylglyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.), etc. Examples include esters and synthetic triglycerides.

Examples of surfactants include polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, Nonionic surfactants such as polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates , polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, anionic surfactants such as polyoxyethylene alkylphenyl ether phosphate; quaternary ammonium salts, primary to tertiary fatty amine salts, Cationic surfactants such as trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, polyethylenepolyamine fatty acid amide salts; N , N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N',N'-dimethyl-N' Amphoteric surfactants such as -β-hydroxypropylammoniosulfobetaine and the like can be used.

Examples of emulsifiers and/or emulsifying aids include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or salts thereof (such as sodium), complexes of sugar and protein, sucrose or its esters, lactose, soybean-derived Water-soluble polysaccharides, complexes of soybean-derived protein and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and Derivatives thereof, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented grains (barley, beans, cereals, etc.) and the like can also be blended.

Moisturizers include, for example, glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose and raffinose, and mucopolysaccharides ( For example, hyaluronic acid or its salts or derivatives thereof, hyaluronic acid hydrolyzate, chondroitin or its derivatives, heparin or its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, lactic acid, urea, higher fatty acid octyldodecyl and the like. be done.

Examples of thickening agents include alginic acid, agar, carrageenan, fucoidan, and other brown algae, green algae, or red algae-derived components; pectin, polysaccharides, such as aloe polysaccharide; gums, such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; Cellulose derivatives such as methylcellulose, hydroxyethylcellulose, hydroxypropylcellulose; synthetic polymers such as carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, acrylic acid/methacrylic acid copolymer; hyaluronic acid and its derivatives ; polyglutamic acid and its derivatives, polyacrylic acid, and the like.

Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin. or derivatives thereof.

Examples of antiseptic/bactericidal agents include urea; paraoxybenzoic acid esters such as benzoic acid or its salts, methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, and hexachlorophene. , chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, alkylisoquinolinium bromide, resorcinol, jamal (imidazodenyl urea), isopropylmethyl Essential oils such as phenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, hexanediol, concentrated benzalkonium chloride solution 50, mint oil, eucalyptus oil, etc. .

Cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate and the like.

Powder components include, for example, sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk powder, There are cellulosic powders, powders of grains (rice, wheat, corn, millet, etc.), and powders of beans (soybeans, adzuki beans, etc.).

UV absorbers include, for example, ethyl para-aminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Examples of antioxidants include carotenoids such as butylhydroxyanisole, butylhydroxytoluene, propyl gallate, astaxanthin, vitamin E and derivatives thereof (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and derivatives thereof (palmitin acid retinol, etc.).

In addition, as whitening agents, ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamins E and derivatives thereof, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol, and examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid and 2-acetoxy-5-hydroxybenzoic acid. , 2-hydroxy-5-propionyloxybenzoic acid and the like, and α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like. kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5, 5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acids One or more selected from a derivative, an extract of Sohakuhi, a chamomile extract, a hydrolyzate of a rice bran extract, a saxifrage extract and a white mustard extract, or a hydrolyzate thereof.

Examples of the above kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate and kojic acid dibutyrate; kojic acid sugar derivatives such as kojic acid ethers and kojic acid glucoside; etc., ascorbic acid derivatives such as sodium L-ascorbic acid-2-phosphate, magnesium L-ascorbic acid-2-phosphate, sodium L-ascorbic acid-2-sulfate, L-ascorbic acid- Ascorbic acid ester salts such as 2-sulfate magnesium, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl potassium phosphate, myristyl 3-glyceryl ascorbate, Ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L -L-ascorbic acid tetrafatty acid esters such as ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acyl thereof derivatives, ascorbic acid glycerol derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbate phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate etc., as hydroquinone derivatives, arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), etc., and as tranexamic acid derivatives, tranexamic acid esters (eg, tranexamic acid lauryl ester , tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), tranexamic acid amides (e.g., tranexamic acid methylamide), etc., and resorcinol derivatives such as 4-n-butylresorcinol, 4-isoamyl Resorcinol and the like, 2,5-dihydroxybenzoic acid derivatives such as 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, and nicotinic acid derivatives Examples include nicotinamide (niacinamide) and benzyl nicotinate, and α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like.

In addition, as anti-wrinkle agents, vitamin A or derivatives thereof, niacinamide, vitamin E or derivatives thereof (tocopherol acetate, etc.), vitamin C or derivatives thereof (ascorbic acid glucoside, 3-O-ethylascorbic acid, ascorbic acid phosphate magnesium salts, etc.), pantothenyl alcohol, tranexamic acid, and the like.

Furthermore, it is also possible to use together the components derived from natural products, such as the following plants or microorganisms. For example, collagen or its hydrolyzate, yeast extract or hydrolyzate, lactic acid bacteria culture, gramineous plant, cruciferous plant, camellia plant, rosaceous plant, peonies plant, citrus family plant, Amaraceae plant, eelgrass plants, leguminous plants, Asteraceae plants, leguminous plants, mallow plants, Gentian plants, Labiatae plants, Lotus plants, Cucurbitaceous plants, Araliaceae plants, Solanaceous plants, Bignoniaceae plants, Actinidia plants , Moraceae plants, Iridaceae plants, Bellflowers plants, Oleaceae plants, Actinidia plants, Moraceae plants, buckthorn plants, Orchidaceae plants, Anacardiaceae plants, Garcinae plants, Valenciaceae plants, Rutaceae plants, Myrtaceae An extract or hydrolyzate or fermented product thereof from one or more plants selected from the family plants of the family Liliaceae, plants of the family Crassulaceae, plants of the Cupressaceae family, plants of the Convolvulaceae family, and plants of the family Astragalus family, Laminaceae, Myrrhaceae and one or more seaweed extracts, hydrolysates, or fermented products thereof selected from any of Ulna family, jellyfish (self-lysed products such as moon jellyfish and Echizen jellyfish), hydrolysates or fermented products of hyaluronic acid, and royal jelly or its hydrolyzate or fermented product.

Next, the present invention will be described in more detail with Production Examples, Test Examples and Formulation Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all percentages mean weight %.

Production example 1. Preparation of Fermented Coix Seed Solution 50 g of Coix seed from which the husk was removed was pulverized, 950 g of purified water was added to prepare a suspension, and heat sterilization was performed. After 0.5 g of glucoamylase and 0.5 g of papain were added to this suspension, yeast (Saccharomyces cerevisiae) was inoculated at 10 ⁷ cells/mL and statically cultured at 37° C. for 3 days. After culturing, the culture was sterilized by heating, cooled to room temperature, and filtered to obtain 500 g of a fermented coix seed solution (solid concentration: 1.0%).

Production example 2. Preparation of Salicornia herbacea Extract Solution 200 g of purified water was added to 20 g of dried shredded whole plant of Salicornia herbacea and extracted at 40° C. for 1 hour. The resulting extract was filtered to obtain 155 g of a brown transparent extract solution (solid content: 2.00%).

Production example 3. Preparation of Aspalathus linearis extract solution To 100 g of dried whole plant of unfermented rooibos of Aspalathus linearis, which is a plant belonging to the genus Aspalathus of the legume family, was added purified water and 1,3-butylene glycol (7:3). ) was added, and after extraction at 80° C. for 2 hours, insoluble matter was removed by filtration to obtain 1025 g of a dark brown green rooibos extract (solid concentration: 1.50%).

The fermented product solution of Production Example 1 and the extract solutions of Production Examples 2 and 3 prepared as described above were added to the optimum solids concentration ratio, that is, the fermented coix seed solution (Production Example 1): Salicornia extract solution (Manufacturing Example 2): Aspalathus linearis extract solution (Manufacturing Example 3) = 5.5 to 6.0: 3.0 to 3.5: 1, and the mixture is The following test example 1 and formulation examples are shown below as "compositions".

Test example 1. Gene Evaluation Test for Moisturizing-Related Proteins in Epidermis Normal human epidermal cells NHEK were seeded in a 24-well microplate and cultured in Humedia KG2 medium. After culturing for 24 hours, a medium supplemented with the composition according to the present invention as a sample solution was added to the culture medium. Here, as sample solutions, solutions having final concentrations of 1.0% and 2.0%, respectively, relative to the medium were prepared and used. After culturing for 24 hours, the cells were collected with 500 µL of Trizol reagent (manufactured by Invitrogen). 100 μL of chloroform was added to the collected epidermal cells, mixed by stirring, and centrifuged at 12,500 rpm and 4° C. for 15 minutes, and then 200 μL of the aqueous layer alone was collected. 250 μL of isopropanol was added to the collected aqueous layer, mixed by stirring, and centrifuged at 12,500 rpm at 4° C. for 10 minutes to obtain a total RNA precipitate. The total RNA was washed by adding 1 mL of 75% cold ethanol and stirring, and centrifuged at 12,500 rpm for 10 minutes at 4°C to collect the precipitate. The recovered total RNA was reverse transcribed using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (manufactured by Takara Bio Inc.)) to synthesize cDNA. Target gene ^expression and internal Detection of expression of the standard GAPDH gene was performed. The test results were obtained by calculating the relative expression level of the target gene with respect to the expression level of the GAPDH gene, and expressed as a relative ratio with the control value being 100.

The results of Test Example 1 are shown in FIGS. 1 to 3. FIG. As shown in FIGS. 1 to 3, the composition according to the present invention expresses the filaggrin gene, which is the source of natural moisturizing factor (NMF), converts the arginine residue to citrulline among filaggrin amino acids, peptidyl arginine It was confirmed that the expression of deiminase-1 (PADI-1) gene and peptidylarginine deiminase-3 (PADI-3) gene is significantly promoted. From this, it can be expected that the external preparation for skin containing the composition of the present invention promotes the production of amino acids, which are natural moisturizing factors (NMF) in the stratum corneum, keeps the skin moisturized, and prevents dryness. .

Hereinafter, formulation examples of lotions, milky lotions, creams and the like are shown as formulation examples of external skin preparations containing the composition according to the present invention, but the present invention is not limited to them.

Formulation example 1. Lotion [Ingredients] Part Jojoba oil 1.0
Compositions of the Invention 3.0
Glycerin 5.0
L-ascorbic acid 2-glucoside 2.0
1,3-butylene glycol 1.0
Water-soluble collagen 0.01
Propanediol 3.0
Potassium hydroxide Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 2. Lotion [Ingredients] Part Squalane 1.0
Compositions of the Invention 3.0
Glycerin 5.0
Arbutin 3.0
1,3-butylene glycol 1.0
Hydrolyzed collagen 0.1
Pentanediol 3.0
Sodium citrate Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 3. Lotion [Ingredients] Part Hydrogenated castor oil 1.0
Compositions of the Invention 3.0
Glycerin 5.0
3-O-ethylascorbic acid 2.0
Tocopherol acetate 0.3
Water-soluble collagen 0.01
1,3-butylene glycol 1.0
Pentanediol 3.0
Potassium hydroxide Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 4. Milky lotion [ingredients] part squalane 5.0
Jojoba oil 1.0
Orange peel oil 1.0
Polyoxyethylene (20) sorbitan monostearate 1.0
Lipophilic glyceryl stearate 1.0
Hydrogenated soybean lecithin 1.5
Composition of the Invention 1.0
Niacinamide 5.0
Glycerin 3.0
Carboxymethyl cellulose 0.3
Xanthan gum 0.2
Sodium hyaluronate 0.01
1,3-butylene glycol 0.1
Phenoxyethanol 0.5
Pentylene glycol 0.5
Diglycerin 0.3
Purified water Amount that makes the total amount 100 parts

Formulation example 5. Milky lotion [ingredients] part squalane 5.0
Castor oil 1.0
Lipophilic glyceryl stearate 1.0
Hydrogenated soybean lecithin 1.5
Composition of the Invention 1.0
Tranexamic acid 2.0
Glycerin 3.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Hydrolyzed hyaluronic acid 0.01
1,3-butylene glycol 0.1
Phenoxyethanol 0.5
Pentylene glycol 0.5
Diglycerin 0.3
Purified water Amount that makes the total amount 100 parts

Formulation example 6. Cream [Ingredients] Part Olive oil 5.0
Jojoba oil 5.0
Squalane 5.0
Palmitic acid 2.5
Composition of the Invention 1.0
Lactic acid bacteria fermented rice 2.0
Hydrogenated lecithin 0.5
Carboxy vinyl polymer 0.3
Sodium alginate 0.2
Water-soluble collagen 0.1
1,3-butylene glycol 0.1
Phenoxyethanol 0.5
Pentylene glycol 0.5
Diglycerin 0.3
Purified water Amount that makes the total amount 100 parts

Formulation example 7. Face wash [Ingredients] Part Glyceryl stearate 2.5
Squalane 3.0
Glycerin 3.0
Polyglyceryl-10 Laurate 3.0
Compositions of the Invention 3.0
Water-soluble collagen 0.01
Sodium hyaluronate 0.01
Isopropylmethylphenol 0.1
1,3-butylene glycol 2.0
Purified water Amount that makes the total amount 100 parts

Claims (9)

Mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract (solid concentration ratio of fermented Coix seed: Salicornia extract: Aspalathus linearis extract = 5.5 to 6.0: 3.0 ~3.5:1) as an active ingredient. Mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract (solid concentration ratio of fermented Coix seed: Salicornia extract: Aspalathus linearis extract = 5.5 to 6.0: 3.0 ~3.5: 1) as an active ingredient and a filaggrin gene expression promoter. An external skin preparation containing 0.01 to 10% by weight or more of the filaggrin gene expression promoter according to claim 2. A mixture of a mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract (the solid concentration ratio is fermented Coix seed: Salicornia extract: Aspalathus linearis extract = 5.5 to 6.0: 3 .0-3.5:1) as an active ingredient. A mixture of a mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract (the solid concentration ratio is fermented Coix seed: Salicornia extract: Aspalathus linearis extract = 5.5 to 6.0: 3 .0-3.5:1) as an active ingredient and a peptidylarginine deiminase-1 (PADI-1) gene expression promoter. An external skin preparation containing 0.01 to 10% by weight or more of the peptidylarginine deiminase-1 (PADI-1) gene expression promoter according to claim 5. Mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract (solid concentration ratio of fermented Coix seed: Salicornia extract: Aspalathus linearis extract = 5.5 to 6.0: 3.0 ~3.5:1) as an active ingredient, a peptidylarginine deiminase-3 (PADI-3) gene expression promoter. Mixture of fermented Coix seed, Salicornia extract and Aspalathus linearis extract (solid concentration ratio of fermented Coix seed: Salicornia extract: Aspalathus linearis extract = 5.5 to 6.0: 3.0 ~3.5:1) as an active ingredient and a peptidylarginine deiminase-3 (PADI-3) gene expression promoter. An external skin preparation containing 0.01 to 10% by weight or more of the peptidylarginine deiminase-3 (PADI-3) gene expression promoter according to claim 8.