JP2023089967A - 蛍光顕微鏡システムおよび方法 - Google Patents

蛍光顕微鏡システムおよび方法 Download PDF

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Publication number
JP2023089967A
JP2023089967A JP2022199971A JP2022199971A JP2023089967A JP 2023089967 A JP2023089967 A JP 2023089967A JP 2022199971 A JP2022199971 A JP 2022199971A JP 2022199971 A JP2022199971 A JP 2022199971A JP 2023089967 A JP2023089967 A JP 2023089967A
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JP
Japan
Prior art keywords
pixels
fluorescence microscope
microscope system
raw image
luminance
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Pending
Application number
JP2022199971A
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English (en)
Japanese (ja)
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JP2023089967A5 (enExample
Inventor
リッチェル カイ
Ritschel Kai
シュリッカー オリヴァー
Schlicker Oliver
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leica Microsystems CMS GmbH
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Leica Microsystems CMS GmbH
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Publication date
Application filed by Leica Microsystems CMS GmbH filed Critical Leica Microsystems CMS GmbH
Publication of JP2023089967A publication Critical patent/JP2023089967A/ja
Publication of JP2023089967A5 publication Critical patent/JP2023089967A5/ja
Pending legal-status Critical Current

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/40—Image enhancement or restoration using histogram techniques
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/90—Determination of colour characteristics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
JP2022199971A 2021-12-16 2022-12-15 蛍光顕微鏡システムおよび方法 Pending JP2023089967A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21215122.9A EP4198601B1 (en) 2021-12-16 2021-12-16 Fluorescence microscope system and method
EP21215122 2021-12-16

Publications (2)

Publication Number Publication Date
JP2023089967A true JP2023089967A (ja) 2023-06-28
JP2023089967A5 JP2023089967A5 (enExample) 2025-12-22

Family

ID=78918750

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022199971A Pending JP2023089967A (ja) 2021-12-16 2022-12-15 蛍光顕微鏡システムおよび方法

Country Status (4)

Country Link
US (1) US12625357B2 (enExample)
EP (1) EP4198601B1 (enExample)
JP (1) JP2023089967A (enExample)
CN (1) CN116266013A (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7280107B2 (ja) * 2019-05-10 2023-05-23 株式会社エビデント 画像処理方法、プログラム、画像処理装置、画像処理システム、及び、顕微鏡システム

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0969936A (ja) * 1995-09-01 1997-03-11 Minolta Co Ltd 画像処理装置
JP2002102146A (ja) * 2000-09-29 2002-04-09 Fuji Photo Film Co Ltd 蛍光画像表示方法および装置
DE10053301A1 (de) * 2000-10-27 2002-05-08 Philips Corp Intellectual Pty Verfahren zur farblichen Wiedergabe eines Graustufenbildes
DE60202588T2 (de) * 2002-02-22 2006-01-05 Agfa-Gevaert N.V. Verfahren zur Rauschminderung
JP2006271870A (ja) * 2005-03-30 2006-10-12 Olympus Medical Systems Corp 内視鏡用画像処理装置
GB201007055D0 (en) * 2010-04-28 2010-06-09 Vib Vzw Method and apparatus for the imaging of a labelled sample
EP2726931A4 (en) * 2011-06-30 2015-04-01 Ge Healthcare Bio Sciences IMAGE QUALITY OPTIMIZATION IN BIOLOGICAL IMAGING
US10244182B2 (en) * 2016-06-23 2019-03-26 Semiconductor Components Industries, Llc Methods and apparatus for reducing spatial flicker artifacts
EP4010881B1 (en) * 2019-08-07 2026-04-15 Magic Leap, Inc. Fast 3d reconstruction with depth information
US11935492B2 (en) * 2020-04-28 2024-03-19 Dolby Laboratories Licensing Corporation Image-dependent contrast and brightness control for HDR displays
EP3907497B1 (en) * 2020-05-08 2023-08-02 Leica Microsystems CMS GmbH Apparatus and method for displaying and/or printing images of a specimen including a fluorophore
CN112651907A (zh) * 2021-02-10 2021-04-13 江苏启阳半导体技术有限公司 一种用于图像处理的直方图均衡实现方法及其系统

Also Published As

Publication number Publication date
EP4198601B1 (en) 2025-08-27
EP4198601A1 (en) 2023-06-21
CN116266013A (zh) 2023-06-20
US20230196513A1 (en) 2023-06-22
US12625357B2 (en) 2026-05-12

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