CN116266013A - 荧光显微镜系统和方法 - Google Patents

荧光显微镜系统和方法 Download PDF

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Publication number
CN116266013A
CN116266013A CN202211622398.3A CN202211622398A CN116266013A CN 116266013 A CN116266013 A CN 116266013A CN 202211622398 A CN202211622398 A CN 202211622398A CN 116266013 A CN116266013 A CN 116266013A
Authority
CN
China
Prior art keywords
pixels
microscope system
fluorescence microscope
image
processor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211622398.3A
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English (en)
Chinese (zh)
Inventor
凯·瑞特舍尔
奥利弗·施利克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leica Microsystems CMS GmbH
Original Assignee
Leica Microsystems CMS GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leica Microsystems CMS GmbH filed Critical Leica Microsystems CMS GmbH
Publication of CN116266013A publication Critical patent/CN116266013A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/40—Image enhancement or restoration using histogram techniques
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/90—Determination of colour characteristics
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
CN202211622398.3A 2021-12-16 2022-12-16 荧光显微镜系统和方法 Pending CN116266013A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21215122.9A EP4198601B1 (en) 2021-12-16 2021-12-16 Fluorescence microscope system and method
EP21215122.9 2021-12-16

Publications (1)

Publication Number Publication Date
CN116266013A true CN116266013A (zh) 2023-06-20

Family

ID=78918750

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211622398.3A Pending CN116266013A (zh) 2021-12-16 2022-12-16 荧光显微镜系统和方法

Country Status (4)

Country Link
US (1) US12625357B2 (enExample)
EP (1) EP4198601B1 (enExample)
JP (1) JP2023089967A (enExample)
CN (1) CN116266013A (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7280107B2 (ja) * 2019-05-10 2023-05-23 株式会社エビデント 画像処理方法、プログラム、画像処理装置、画像処理システム、及び、顕微鏡システム

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0969936A (ja) * 1995-09-01 1997-03-11 Minolta Co Ltd 画像処理装置
JP2002102146A (ja) * 2000-09-29 2002-04-09 Fuji Photo Film Co Ltd 蛍光画像表示方法および装置
DE10053301A1 (de) * 2000-10-27 2002-05-08 Philips Corp Intellectual Pty Verfahren zur farblichen Wiedergabe eines Graustufenbildes
DE60202588T2 (de) * 2002-02-22 2006-01-05 Agfa-Gevaert N.V. Verfahren zur Rauschminderung
JP2006271870A (ja) * 2005-03-30 2006-10-12 Olympus Medical Systems Corp 内視鏡用画像処理装置
GB201007055D0 (en) * 2010-04-28 2010-06-09 Vib Vzw Method and apparatus for the imaging of a labelled sample
EP2726931A4 (en) * 2011-06-30 2015-04-01 Ge Healthcare Bio Sciences IMAGE QUALITY OPTIMIZATION IN BIOLOGICAL IMAGING
US10244182B2 (en) * 2016-06-23 2019-03-26 Semiconductor Components Industries, Llc Methods and apparatus for reducing spatial flicker artifacts
EP4010881B1 (en) * 2019-08-07 2026-04-15 Magic Leap, Inc. Fast 3d reconstruction with depth information
US11935492B2 (en) * 2020-04-28 2024-03-19 Dolby Laboratories Licensing Corporation Image-dependent contrast and brightness control for HDR displays
EP3907497B1 (en) * 2020-05-08 2023-08-02 Leica Microsystems CMS GmbH Apparatus and method for displaying and/or printing images of a specimen including a fluorophore
CN112651907A (zh) * 2021-02-10 2021-04-13 江苏启阳半导体技术有限公司 一种用于图像处理的直方图均衡实现方法及其系统

Also Published As

Publication number Publication date
EP4198601B1 (en) 2025-08-27
EP4198601A1 (en) 2023-06-21
US20230196513A1 (en) 2023-06-22
US12625357B2 (en) 2026-05-12
JP2023089967A (ja) 2023-06-28

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