JP2022539739A - マイクロウェルからの標的物質の回収システムおよび方法 - Google Patents

マイクロウェルからの標的物質の回収システムおよび方法 Download PDF

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Publication number
JP2022539739A
JP2022539739A JP2021577040A JP2021577040A JP2022539739A JP 2022539739 A JP2022539739 A JP 2022539739A JP 2021577040 A JP2021577040 A JP 2021577040A JP 2021577040 A JP2021577040 A JP 2021577040A JP 2022539739 A JP2022539739 A JP 2022539739A
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Japan
Prior art keywords
adapter
region
magnet
capture
target material
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Japanese (ja)
Inventor
ハンディーク，カリヤン
ラナーデ、スワティ
シャルマ，ヴィシャール
ペイン，オースティン
タック，サム
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バイオ－ラッド ラボラトリーズ インコーポレイテッド
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Priority claimed from US16/867,235 external-priority patent/US10900032B2/en
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Publication of JP2022539739A publication Critical patent/JP2022539739A/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1053—General features of the devices using the transfer device for another function for separating part of the liquid, e.g. filters, extraction phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Fluid Mechanics (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021577040A 2019-06-26 2020-06-19 マイクロウェルからの標的物質の回収システムおよび方法 Pending JP2022539739A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962866726P 2019-06-26 2019-06-26
US62/866,726 2019-06-26
US16/867,235 2020-05-05
US16/867,235 US10900032B2 (en) 2019-05-07 2020-05-05 System and method for automated single cell processing
PCT/US2020/038647 WO2020263700A1 (en) 2019-06-26 2020-06-19 System and method for target material retrieval from microwells

Publications (1)

Publication Number Publication Date
JP2022539739A true JP2022539739A (ja) 2022-09-13

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JP2021577040A Pending JP2022539739A (ja) 2019-06-26 2020-06-19 マイクロウェルからの標的物質の回収システムおよび方法

Country Status (8)

Country Link
EP (1) EP3990926A4 (zh)
JP (1) JP2022539739A (zh)
KR (1) KR20220024734A (zh)
CN (1) CN113924494A (zh)
AU (1) AU2020304355B2 (zh)
CA (1) CA3138949A1 (zh)
SG (1) SG11202112150RA (zh)
WO (1) WO2020263700A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023188896A1 (ja) * 2022-03-29 2023-10-05 ソニーグループ株式会社 生体粒子解析システム、情報処理装置、及び生体粒子解析方法

Citations (7)

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Publication number Priority date Publication date Assignee Title
JP2011127965A (ja) * 2009-12-16 2011-06-30 Hitachi High-Technologies Corp 試料処理装置、試料処理方法及びこれらに使用する反応容器
JP2011234671A (ja) * 2010-05-11 2011-11-24 Hitachi High-Technologies Corp 核酸抽出装置
WO2011162290A1 (ja) * 2010-06-22 2011-12-29 ユニバーサル・バイオ・リサーチ株式会社 生体関連物質の捕捉装置および生体関連物質の採取システム
US20130210127A1 (en) * 2012-02-13 2013-08-15 Molecular Systems Corporation System and method for processing and detecting nucleic acids
WO2014007074A1 (ja) * 2012-07-06 2014-01-09 株式会社 日立ハイテクノロジーズ 核酸抽出用キット及び核酸抽出装置
WO2017095917A1 (en) * 2015-12-01 2017-06-08 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
JP2018508187A (ja) * 2015-01-13 2018-03-29 ギルソン インコーポレイテッド 摺動式磁性粒子分離のためのアダプタ

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FI102906B1 (fi) * 1998-02-23 1999-03-15 Bio Nobile Oy Menetelmä ja väline aineen siirtämiseksi
US20050106667A1 (en) * 2003-08-01 2005-05-19 Genentech, Inc Binding polypeptides with restricted diversity sequences
CN103499466B (zh) * 2006-01-18 2017-04-12 阿戈斯治疗公司 用于处理封闭容器中的样品的系统和方法以及相关装置
EP2229441B1 (en) * 2007-12-12 2014-10-01 The Board of Trustees of The Leland Stanford Junior University Method and apparatus for magnetic separation of cells
CA2757300C (en) * 2009-04-01 2018-01-09 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (clpa)
US9174216B2 (en) * 2013-03-13 2015-11-03 DeNovo Science, Inc. System for capturing and analyzing cells
US9707562B2 (en) * 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
EP3651903A4 (en) * 2017-08-29 2021-06-16 Bio-Rad Laboratories, Inc. SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011127965A (ja) * 2009-12-16 2011-06-30 Hitachi High-Technologies Corp 試料処理装置、試料処理方法及びこれらに使用する反応容器
JP2011234671A (ja) * 2010-05-11 2011-11-24 Hitachi High-Technologies Corp 核酸抽出装置
WO2011162290A1 (ja) * 2010-06-22 2011-12-29 ユニバーサル・バイオ・リサーチ株式会社 生体関連物質の捕捉装置および生体関連物質の採取システム
US20130210127A1 (en) * 2012-02-13 2013-08-15 Molecular Systems Corporation System and method for processing and detecting nucleic acids
WO2014007074A1 (ja) * 2012-07-06 2014-01-09 株式会社 日立ハイテクノロジーズ 核酸抽出用キット及び核酸抽出装置
JP2018508187A (ja) * 2015-01-13 2018-03-29 ギルソン インコーポレイテッド 摺動式磁性粒子分離のためのアダプタ
WO2017095917A1 (en) * 2015-12-01 2017-06-08 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes

Also Published As

Publication number Publication date
CN113924494A (zh) 2022-01-11
EP3990926A1 (en) 2022-05-04
WO2020263700A1 (en) 2020-12-30
AU2020304355B2 (en) 2023-04-06
SG11202112150RA (en) 2021-12-30
KR20220024734A (ko) 2022-03-03
EP3990926A4 (en) 2023-09-13
AU2020304355A1 (en) 2021-11-25
CA3138949A1 (en) 2020-12-30

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