JP2022515221A - クロマチンマッピングアッセイ用のｄｎａバーコード化ヌクレオソーム - Google Patents

クロマチンマッピングアッセイ用のｄｎａバーコード化ヌクレオソーム Download PDF

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JP2022515221A
JP2022515221A JP2021536186A JP2021536186A JP2022515221A JP 2022515221 A JP2022515221 A JP 2022515221A JP 2021536186 A JP2021536186 A JP 2021536186A JP 2021536186 A JP2021536186 A JP 2021536186A JP 2022515221 A JP2022515221 A JP 2022515221A
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nucleosome
dna
polynucleosome
transposase
chromatin
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JP2022515221A5 (enExample
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ダブリュー． コウルズ，マルティス
サン，ズー－ウェン
キーオ，マイケル－クリストファー
ジェイコブ ヴェンターズ，ブライアン
ニコル ウエインザップフェル，エレン
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Epicypher Inc
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Epicypher Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
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  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
JP2021536186A 2018-12-21 2019-12-20 クロマチンマッピングアッセイ用のｄｎａバーコード化ヌクレオソーム Pending JP2022515221A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862783861P 2018-12-21 2018-12-21
US62/783,861 2018-12-21
PCT/US2019/067735 WO2020132388A1 (en) 2018-12-21 2019-12-20 Dna-barcoded nucleosomes for chromatin mapping assays

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JP2022515221A true JP2022515221A (ja) 2022-02-17
JP2022515221A5 JP2022515221A5 (enExample) 2024-06-13

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JP2021536186A Pending JP2022515221A (ja) 2018-12-21 2019-12-20 クロマチンマッピングアッセイ用のｄｎａバーコード化ヌクレオソーム

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US (2) US12460246B2 (enExample)
EP (1) EP3899042A4 (enExample)
JP (1) JP2022515221A (enExample)
CN (1) CN113508180A (enExample)
AU (1) AU2019403351A1 (enExample)
CA (1) CA3124482A1 (enExample)
WO (1) WO2020132388A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102816410B1 (ko) * 2019-02-13 2025-06-04 프로바이오겐 아게 향상된 삽입 부위 선택 특성을 지닌 트랜스포사제(transposase)
WO2022056309A1 (en) * 2020-09-11 2022-03-17 Fred Hutchinson Cancer Research Center Improved high efficiency targeted in situ genome-wide profiling
EP4237850A4 (en) * 2020-11-02 2024-09-18 Epicypher, Inc. IMPROVED ASSAYS FOR QUANTIFYING CHROMATIN TARGETS USING ANTIBODY-TARGETED ENZYMATIC DIGESTION
WO2024208912A1 (en) * 2023-04-03 2024-10-10 Belgian Volition Srl Recombinant nucleosome materials
WO2025083285A1 (en) * 2023-10-20 2025-04-24 Norwegian University Of Science And Technology (Ntnu) Methods and products for use in genome-wide chromatin profiling

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015521462A (ja) * 2012-06-06 2015-07-30 ザ、トラスティーズ オブ プリンストン ユニバーシティ デザイナーモノヌクレオソーム（ｄｅｓｉｇｎｅｒｍｏｎｏｎｕｃｌｅｏｓｏｍｅ）のＤＮＡバーコード化ならびにクロマチンリーダー、ライター、イレーサーおよびそのモジュレーターをプロファイリングするためのクロマチンアレイライブラリー
JP2017506073A (ja) * 2014-02-03 2017-03-02 ザ ユニバーシティー オブ シカゴ Ｄｎａ−蛋白質複合体の組成及びその密度の定量評価方法

Family Cites Families (5)

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Publication number Priority date Publication date Assignee Title
US20160208323A1 (en) * 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing
WO2016161314A1 (en) * 2015-04-03 2016-10-06 Dana-Farber Cancer Institute, Inc. Chromatin remodeling activity assays and uses thereof
SG11201807117WA (en) * 2016-02-23 2018-09-27 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing
US11215622B2 (en) * 2017-01-27 2022-01-04 Covaris, Inc. Generation of cfDNA reference material
GB201704402D0 (en) * 2017-03-20 2017-05-03 Blacktrace Holdings Ltd Single cell DNA sequencing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015521462A (ja) * 2012-06-06 2015-07-30 ザ、トラスティーズ オブ プリンストン ユニバーシティ デザイナーモノヌクレオソーム（ｄｅｓｉｇｎｅｒｍｏｎｏｎｕｃｌｅｏｓｏｍｅ）のＤＮＡバーコード化ならびにクロマチンリーダー、ライター、イレーサーおよびそのモジュレーターをプロファイリングするためのクロマチンアレイライブラリー
JP2017506073A (ja) * 2014-02-03 2017-03-02 ザ ユニバーシティー オブ シカゴ Ｄｎａ−蛋白質複合体の組成及びその密度の定量評価方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ADRIAN T. GRZYBOWSKI: "Calibrating ChIP-Seq with Nucleosomal Internal Standards to Measure Histone Modi?cation De", MOL CELL., vol. 58, JPN6023050056, 2015, pages 886 - 99, ISSN: 0005426067 *
HATICE S. KAYA-OKUR: "CUT&Tag for efficient epigenomic profiling of small samples and single cells.", NAT COMMUN., vol. 10, JPN6023050060, 2019, pages 1930, ISSN: 0005211428 *
RENE A. M. DIRKS,: "Genome-wide epigenomic profiling for biomarker discovery", CLINICAL EPIGENETICS, vol. 8, JPN6023050059, 2016, pages 122, ISSN: 0005211427 *
SKENE, PETER J: "Targeted in situ genome-wide profiling with high efficiency for low cell numbers.", NAT PROTOC., vol. 13, JPN6023050057, 2018, pages 1006 - 19, ISSN: 0005426068 *
越阪部 晃永: "メチル化DNAを含むヌクレオソームの構造生物学的解析", 科研費データベース, JPN6023050058, 2017, pages 1 - 5, ISSN: 0005426069 *

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US12460246B2 (en) 2025-11-04
EP3899042A4 (en) 2022-10-12
AU2019403351A1 (en) 2021-08-12
US20220042074A1 (en) 2022-02-10
CN113508180A (zh) 2021-10-15
EP3899042A1 (en) 2021-10-27
CA3124482A1 (en) 2020-06-25
WO2020132388A1 (en) 2020-06-25
US20260035731A1 (en) 2026-02-05

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