CA3124482A1 - Dna-barcoded nucleosomes for chromatin mapping assays - Google Patents
Dna-barcoded nucleosomes for chromatin mapping assays Download PDFInfo
- Publication number
- CA3124482A1 CA3124482A1 CA3124482A CA3124482A CA3124482A1 CA 3124482 A1 CA3124482 A1 CA 3124482A1 CA 3124482 A CA3124482 A CA 3124482A CA 3124482 A CA3124482 A CA 3124482A CA 3124482 A1 CA3124482 A1 CA 3124482A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleosome
- polynucleosome
- dna
- cell
- panel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862783861P | 2018-12-21 | 2018-12-21 | |
| US62/783,861 | 2018-12-21 | ||
| PCT/US2019/067735 WO2020132388A1 (en) | 2018-12-21 | 2019-12-20 | Dna-barcoded nucleosomes for chromatin mapping assays |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3124482A1 true CA3124482A1 (en) | 2020-06-25 |
Family
ID=71100573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3124482A Pending CA3124482A1 (en) | 2018-12-21 | 2019-12-20 | Dna-barcoded nucleosomes for chromatin mapping assays |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US12460246B2 (enExample) |
| EP (1) | EP3899042A4 (enExample) |
| JP (1) | JP2022515221A (enExample) |
| CN (1) | CN113508180A (enExample) |
| AU (1) | AU2019403351A1 (enExample) |
| CA (1) | CA3124482A1 (enExample) |
| WO (1) | WO2020132388A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102816410B1 (ko) * | 2019-02-13 | 2025-06-04 | 프로바이오겐 아게 | 향상된 삽입 부위 선택 특성을 지닌 트랜스포사제(transposase) |
| WO2022056309A1 (en) * | 2020-09-11 | 2022-03-17 | Fred Hutchinson Cancer Research Center | Improved high efficiency targeted in situ genome-wide profiling |
| EP4237850A4 (en) * | 2020-11-02 | 2024-09-18 | Epicypher, Inc. | IMPROVED ASSAYS FOR QUANTIFYING CHROMATIN TARGETS USING ANTIBODY-TARGETED ENZYMATIC DIGESTION |
| WO2024208912A1 (en) * | 2023-04-03 | 2024-10-10 | Belgian Volition Srl | Recombinant nucleosome materials |
| WO2025083285A1 (en) * | 2023-10-20 | 2025-04-24 | Norwegian University Of Science And Technology (Ntnu) | Methods and products for use in genome-wide chromatin profiling |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013184930A2 (en) | 2012-06-06 | 2013-12-12 | Trustees Of Princeton University | Dna barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof |
| US20160208323A1 (en) * | 2013-06-21 | 2016-07-21 | The Broad Institute, Inc. | Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing |
| WO2015117145A1 (en) | 2014-02-03 | 2015-08-06 | The University Of Chicago | Compositions and methods for quantitative assesment of dna-protein complex density |
| WO2016161314A1 (en) * | 2015-04-03 | 2016-10-06 | Dana-Farber Cancer Institute, Inc. | Chromatin remodeling activity assays and uses thereof |
| SG11201807117WA (en) * | 2016-02-23 | 2018-09-27 | Dovetail Genomics Llc | Generation of phased read-sets for genome assembly and haplotype phasing |
| US11215622B2 (en) * | 2017-01-27 | 2022-01-04 | Covaris, Inc. | Generation of cfDNA reference material |
| GB201704402D0 (en) * | 2017-03-20 | 2017-05-03 | Blacktrace Holdings Ltd | Single cell DNA sequencing |
-
2019
- 2019-12-20 CN CN201980092668.4A patent/CN113508180A/zh active Pending
- 2019-12-20 WO PCT/US2019/067735 patent/WO2020132388A1/en not_active Ceased
- 2019-12-20 AU AU2019403351A patent/AU2019403351A1/en active Pending
- 2019-12-20 EP EP19898873.5A patent/EP3899042A4/en active Pending
- 2019-12-20 CA CA3124482A patent/CA3124482A1/en active Pending
- 2019-12-20 JP JP2021536186A patent/JP2022515221A/ja active Pending
- 2019-12-20 US US17/416,928 patent/US12460246B2/en active Active
-
2025
- 2025-10-09 US US19/354,009 patent/US20260035731A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US12460246B2 (en) | 2025-11-04 |
| EP3899042A4 (en) | 2022-10-12 |
| AU2019403351A1 (en) | 2021-08-12 |
| US20220042074A1 (en) | 2022-02-10 |
| CN113508180A (zh) | 2021-10-15 |
| EP3899042A1 (en) | 2021-10-27 |
| JP2022515221A (ja) | 2022-02-17 |
| WO2020132388A1 (en) | 2020-06-25 |
| US20260035731A1 (en) | 2026-02-05 |
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|---|---|---|
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220922 |
|
| MFA | Maintenance fee for application paid |
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| U00 | Fee paid |
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| U11 | Full renewal or maintenance fee paid |
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| D15 | Examination report completed |
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| W00 | Other event occurred |
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| P13 | Application amended |
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| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 6TH ANNIV.) - STANDARD Year of fee payment: 6 |
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| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20251125 |
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| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20251127 |