JP2022500049A - 多価不飽和脂肪酸(pufa)に富むオイルの抽出方法 - Google Patents
多価不飽和脂肪酸(pufa)に富むオイルの抽出方法 Download PDFInfo
- Publication number
- JP2022500049A JP2022500049A JP2021514128A JP2021514128A JP2022500049A JP 2022500049 A JP2022500049 A JP 2022500049A JP 2021514128 A JP2021514128 A JP 2021514128A JP 2021514128 A JP2021514128 A JP 2021514128A JP 2022500049 A JP2022500049 A JP 2022500049A
- Authority
- JP
- Japan
- Prior art keywords
- oil
- biomass
- pufa
- temperature
- oils
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 title claims abstract description 67
- 238000000605 extraction Methods 0.000 title description 19
- 239000003921 oil Substances 0.000 claims abstract description 121
- 238000000034 method Methods 0.000 claims abstract description 63
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 claims abstract description 33
- 235000020669 docosahexaenoic acid Nutrition 0.000 claims abstract description 29
- 229940090949 docosahexaenoic acid Drugs 0.000 claims abstract description 4
- 239000002028 Biomass Substances 0.000 claims description 46
- 239000000203 mixture Substances 0.000 claims description 27
- 235000013305 food Nutrition 0.000 claims description 18
- 244000005700 microbiome Species 0.000 claims description 17
- 239000010779 crude oil Substances 0.000 claims description 15
- 238000004090 dissolution Methods 0.000 claims description 13
- 238000000926 separation method Methods 0.000 claims description 10
- 235000020673 eicosapentaenoic acid Nutrition 0.000 claims description 9
- 238000002844 melting Methods 0.000 claims description 9
- 230000008018 melting Effects 0.000 claims description 9
- 230000002255 enzymatic effect Effects 0.000 claims description 8
- YUFFSWGQGVEMMI-JLNKQSITSA-N (7Z,10Z,13Z,16Z,19Z)-docosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCCCC(O)=O YUFFSWGQGVEMMI-JLNKQSITSA-N 0.000 claims description 7
- 239000008194 pharmaceutical composition Substances 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 4
- 230000002934 lysing effect Effects 0.000 claims description 4
- 238000007670 refining Methods 0.000 claims description 3
- 235000021294 Docosapentaenoic acid Nutrition 0.000 claims description 2
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 claims description 2
- 235000021342 arachidonic acid Nutrition 0.000 claims description 2
- 229940114079 arachidonic acid Drugs 0.000 claims description 2
- 238000007865 diluting Methods 0.000 claims description 2
- 229960005135 eicosapentaenoic acid Drugs 0.000 claims description 2
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 abstract description 14
- 230000000813 microbial effect Effects 0.000 abstract description 8
- 239000000284 extract Substances 0.000 abstract description 4
- 235000019198 oils Nutrition 0.000 description 107
- 241000598397 Schizochytrium sp. Species 0.000 description 33
- 241001298230 Thraustochytrium sp. Species 0.000 description 28
- 101710184216 Cardioactive peptide Proteins 0.000 description 24
- 241000341438 Aurantiochytrium mangrovei Species 0.000 description 23
- 230000009089 cytolysis Effects 0.000 description 17
- 235000014113 dietary fatty acids Nutrition 0.000 description 13
- 229930195729 fatty acid Natural products 0.000 description 13
- 239000000194 fatty acid Substances 0.000 description 13
- 150000004665 fatty acids Chemical class 0.000 description 13
- 241001130339 Aurantiochytrium sp. Species 0.000 description 12
- 241001465754 Metazoa Species 0.000 description 9
- 235000021466 carotenoid Nutrition 0.000 description 8
- 150000001747 carotenoids Chemical class 0.000 description 8
- 241001643316 Thraustochytriaceae sp. Species 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000003595 Aurantiochytrium limacinum Species 0.000 description 6
- 206010057248 Cell death Diseases 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 241000199912 Crypthecodinium cohnii Species 0.000 description 5
- 102000005158 Subtilisins Human genes 0.000 description 5
- 108010056079 Subtilisins Proteins 0.000 description 5
- 235000015872 dietary supplement Nutrition 0.000 description 5
- 239000000546 pharmaceutical excipient Substances 0.000 description 5
- 150000004671 saturated fatty acids Chemical class 0.000 description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- 241000224474 Nannochloropsis Species 0.000 description 4
- 241000003603 Thraustochytrium striatum Species 0.000 description 4
- 235000013361 beverage Nutrition 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000003441 saturated fatty acids Nutrition 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 235000010692 trans-unsaturated fatty acids Nutrition 0.000 description 4
- 150000003626 triacylglycerols Chemical class 0.000 description 4
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 4
- SSZWWUDQMAHNAQ-UHFFFAOYSA-N 3-chloropropane-1,2-diol Chemical compound OCC(O)CCl SSZWWUDQMAHNAQ-UHFFFAOYSA-N 0.000 description 3
- 241001306132 Aurantiochytrium Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 241000233673 Schizochytrium aggregatum Species 0.000 description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241000592344 Spermatophyta Species 0.000 description 3
- 241000003605 Ulkenia visurgensis Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 239000008157 edible vegetable oil Substances 0.000 description 3
- 125000004494 ethyl ester group Chemical group 0.000 description 3
- 235000013350 formula milk Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- CTKINSOISVBQLD-UHFFFAOYSA-N Glycidol Chemical compound OCC1CO1 CTKINSOISVBQLD-UHFFFAOYSA-N 0.000 description 2
- 241000907999 Mortierella alpina Species 0.000 description 2
- 241001250129 Nannochloropsis gaditana Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000003599 Thraustochytrium aggregatum Species 0.000 description 2
- 241000144181 Thraustochytrium aureum Species 0.000 description 2
- 241001501879 Thraustochytrium kinnei Species 0.000 description 2
- 241001501884 Ulkenia profunda Species 0.000 description 2
- 241001298226 Ulkenia sp. Species 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 235000013793 astaxanthin Nutrition 0.000 description 2
- 239000001168 astaxanthin Substances 0.000 description 2
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 2
- 229940022405 astaxanthin Drugs 0.000 description 2
- FDSDTBUPSURDBL-LOFNIBRQSA-N canthaxanthin Chemical compound CC=1C(=O)CCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)CCC1(C)C FDSDTBUPSURDBL-LOFNIBRQSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000021323 fish oil Nutrition 0.000 description 2
- 235000021588 free fatty acids Nutrition 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000007794 irritation Effects 0.000 description 2
- 150000004668 long chain fatty acids Chemical class 0.000 description 2
- 235000020667 long-chain omega-3 fatty acid Nutrition 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 description 2
- 235000011152 sodium sulphate Nutrition 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- MJYQFWSXKFLTAY-OVEQLNGDSA-N (2r,3r)-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]butane-1,4-diol;(2r,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O.C1=C(O)C(OC)=CC(C[C@@H](CO)[C@H](CO)CC=2C=C(OC)C(O)=CC=2)=C1 MJYQFWSXKFLTAY-OVEQLNGDSA-N 0.000 description 1
- JKQXZKUSFCKOGQ-JLGXGRJMSA-N (3R,3'R)-beta,beta-carotene-3,3'-diol Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C[C@@H](O)CC1(C)C JKQXZKUSFCKOGQ-JLGXGRJMSA-N 0.000 description 1
- DYPJJAAKPQKWTM-UHFFFAOYSA-N 2-chloropropane-1,3-diol Chemical compound OCC(Cl)CO DYPJJAAKPQKWTM-UHFFFAOYSA-N 0.000 description 1
- 101710190981 50S ribosomal protein L6 Proteins 0.000 description 1
- 241000195645 Auxenochlorella protothecoides Species 0.000 description 1
- 241000206761 Bacillariophyta Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 244000197813 Camelina sativa Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000195654 Chlorella sorokiniana Species 0.000 description 1
- 241000195651 Chlorella sp. Species 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 101001093025 Geobacillus stearothermophilus 50S ribosomal protein L7/L12 Proteins 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241000371004 Graesiella emersonii Species 0.000 description 1
- 235000019487 Hazelnut oil Nutrition 0.000 description 1
- 241001442242 Heterochlorella luteoviridis Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000208204 Linum Species 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 241000180113 Monodus Species 0.000 description 1
- 241000235575 Mortierella Species 0.000 description 1
- 241001219224 Mortierella elongata Species 0.000 description 1
- 241000133355 Mortierella hygrophila Species 0.000 description 1
- 241000517188 Nannochloropsis limnetica Species 0.000 description 1
- 241000159660 Nannochloropsis oculata Species 0.000 description 1
- 241000224476 Nannochloropsis salina Species 0.000 description 1
- 241000509521 Nannochloropsis sp. Species 0.000 description 1
- 241000195659 Neodesmus pupukensis Species 0.000 description 1
- 241000180701 Nitzschia <flatworm> Species 0.000 description 1
- 241000206745 Nitzschia alba Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 241000195646 Parachlorella kessleri Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000206731 Phaeodactylum Species 0.000 description 1
- 241000206744 Phaeodactylum tricornutum Species 0.000 description 1
- OOUTWVMJGMVRQF-DOYZGLONSA-N Phoenicoxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)C(=O)C(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)C(=O)CCC2(C)C OOUTWVMJGMVRQF-DOYZGLONSA-N 0.000 description 1
- 241000233622 Phytophthora infestans Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000195648 Pseudochlorella pringsheimii Species 0.000 description 1
- 235000019484 Rapeseed oil Nutrition 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 101100154580 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TRR2 gene Proteins 0.000 description 1
- 241000233671 Schizochytrium Species 0.000 description 1
- 241000003597 Schizochytrium minutum Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 235000019486 Sunflower oil Nutrition 0.000 description 1
- 241001467333 Thraustochytriaceae Species 0.000 description 1
- 241001216843 Thraustochytrium gaertnerium Species 0.000 description 1
- 241000323188 Thraustochytrium motivum Species 0.000 description 1
- 241000003601 Thraustochytrium multirudimentale Species 0.000 description 1
- 241000003604 Thraustochytrium pachydermum Species 0.000 description 1
- 241000817756 Thraustochytrium roseum Species 0.000 description 1
- 241000233677 Thraustochytrium visurgense Species 0.000 description 1
- 241001491678 Ulkenia Species 0.000 description 1
- 235000019498 Walnut oil Nutrition 0.000 description 1
- JKQXZKUSFCKOGQ-LQFQNGICSA-N Z-zeaxanthin Natural products C([C@H](O)CC=1C)C(C)(C)C=1C=CC(C)=CC=CC(C)=CC=CC=C(C)C=CC=C(C)C=CC1=C(C)C[C@@H](O)CC1(C)C JKQXZKUSFCKOGQ-LQFQNGICSA-N 0.000 description 1
- QOPRSMDTRDMBNK-RNUUUQFGSA-N Zeaxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCC(O)C1(C)C)C=CC=C(/C)C=CC2=C(C)CC(O)CC2(C)C QOPRSMDTRDMBNK-RNUUUQFGSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
- JKQXZKUSFCKOGQ-LOFNIBRQSA-N all-trans-Zeaxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)CC(O)CC2(C)C JKQXZKUSFCKOGQ-LOFNIBRQSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000010478 argan oil Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 235000015173 baked goods and baking mixes Nutrition 0.000 description 1
- 235000013734 beta-carotene Nutrition 0.000 description 1
- 239000011648 beta-carotene Substances 0.000 description 1
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
- 229960002747 betacarotene Drugs 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000012682 canthaxanthin Nutrition 0.000 description 1
- 239000001659 canthaxanthin Substances 0.000 description 1
- 229940008033 canthaxanthin Drugs 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000013367 dietary fats Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 235000015897 energy drink Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229940013317 fish oils Drugs 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 125000003055 glycidyl group Chemical group C(C1CO1)* 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000010468 hazelnut oil Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000010460 hemp oil Substances 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000944 linseed oil Substances 0.000 description 1
- 235000021388 linseed oil Nutrition 0.000 description 1
- 125000003473 lipid group Chemical group 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 235000012680 lutein Nutrition 0.000 description 1
- 239000001656 lutein Substances 0.000 description 1
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 1
- 229960005375 lutein Drugs 0.000 description 1
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000006014 omega-3 oil Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 238000009372 pisciculture Methods 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000008247 solid mixture Substances 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000002600 sunflower oil Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000019195 vitamin supplement Nutrition 0.000 description 1
- 239000003039 volatile agent Substances 0.000 description 1
- 239000008170 walnut oil Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000004018 waxing Methods 0.000 description 1
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
- 235000010930 zeaxanthin Nutrition 0.000 description 1
- 239000001775 zeaxanthin Substances 0.000 description 1
- 229940043269 zeaxanthin Drugs 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Mycology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Birds (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Nutrition Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Emergency Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Dermatology (AREA)
- Fats And Perfumes (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Edible Oils And Fats (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
当該方法が、
(a)前記オイル産生生物のバイオマスの細胞を溶解する工程と、
(b)溶解されたバイオマスからオイルを機械的に分離し、粗製オイルを回収する工程と
を含み、
前記細胞を溶解する工程(a)が、下記2つのパート:
(a1)第1の温度で実行される第1のパート、次いで
(a2)前記第1の温度よりも低い第2の温度で溶解を実行し続ける第2のパート
を含むことを特徴とする、方法に関する。
培養は、専用の自動操作、およびコンピュータステーションによる監視を備えた1〜5Lの有用な容積を持つ発酵槽(バイオリアクター)中で行った。培養は、Aurantiochytrium mangroveiの2株を用い、2つの異なる培養プロトコルで行った。系は、塩基(実施例b1およびb2についてはNH4OH、実施例aについてはNaOH)の添加によりpH5に調整し、窒素供給を行いながら(実施例b1およびb2について)、培養の全期間にわたってpH調整を行った。培養温度は、30℃、その後22℃、最終的に培養の終了時に18℃とした。
総バイオマス濃度は、乾燥質量を測定することによってモニターした(測定前に、Whatman GF/Fフィルタで濾過し、その後105℃で、最小で24時間オーブン乾燥させた)。脂肪酸分析は、バイオマスについては、ISO12966−2規格に準拠した方法に従って、またオイルについては、欧州薬局方9.0(2.4.29.)に従って行った。
10m3(実施例d)または180m3(実施例e)のタンクなどの工業用サイズの発酵槽中で、培地b、および炭素:窒素:リン(CNP)のモル比率が533:0.4:1である強化溶液の形態でのグルコース添加により、同様の条件で増殖させたとき、高DHA含有Aurantiochytrium mangrovei株は、同様の脂肪酸組成を有するバイオマスを生産する。
実施例2の条件下で生産され、60%超のDHAを含有する、同一のバイオマスからオイルを抽出した(表4)。
以下の手順に従って、実施例2で生産されたバイオマスからオイルを抽出した:
(a)65℃の温度で4時間の、酵素的手段(例えば、Novozymes社製のAlcalase 2.5LまたはAlcalase 2.4LまたはNovozym 37071)による細胞溶解、
(b)5から40℃に温度を下げながら、30分から30時間、引き続き溶解、
(c)遠心プレートセパレータによるオイルの機械的分離。
・EP 0 223 960; EP 1 001 034
・WO 1994/008467; WO 1997/037032; WO 2001/054510; WO 02/10322; WO 03/049832; WO 2010/107415; WO2011/153246; WO 2012/035262; WO 2013/136025; WO 2013/136028; WO 2014/146098; WO 2015/004402; WO 2015/004403; WO2015/095688; WO2015/095694; WO 2015/150716; WO 2016/030631
・US 6,750,048, US 2011/295028, US 2015/176042, US 2014/350222
・Fedorova-Dahms I. & al., Safety evaluation of DHA-rich algal oil from Schizochytrium sp, Food and Chemical Toxicology, 2011, 49, 3310-3318
・Folch J, et al., A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May; 226(1):497-509
・Hamilton M. & al., Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornum, Marine Drugs, 2016, 14, 53
・Omega-3 long chain fatty acid "bioavailability": a review of evidence and methodological considerations.
・Ghasemifard S, Turchini GM, Sinclair AJ. Prog Lipid Res. 2014 Oct;56:92-108. doi: 10.1016/j.plipres.2014.09.001. Epub 2014 Sep 16. Review.
・Wakako TSUZUKI, Study of the Formation of trans Fatty Acids in Model Oils (triacylglycerols) and Edible Oils during the Heating Process , JARQ 46 (3), 215-220 (2012)
・Kinuko Miyazaki* and Kazuo Koyama, An Improved Enzymatic Indirect Method for Simultaneous Determinations of 3-MCPD Esters and Glycidyl Esters in Fish Oils , J. Oleo Sci. 66, (10) 1085-1093 (2017)
・Jouhet J., Marechal E., Bligny R., Joyard J., Block M. A. (2003). Transient increase of phosphatidylcholine in plant cells in response to phosphate deprivation. FEBS Lett. 544 63-68.
・Maged P. Mansour,1,* Pushkar Shrestha,2 Srinivas Belide,2 James R. Petrie,2 Peter D. Nichols,1 and Surinder P. Singh2 (2014). Characterization of Oilseed Lipids from “DHA-Producing Camelina sativa”: A New Transformed Land Plant Containing Long-Chain Omega-3 Oils. Nutrients. 2014 Feb; 6(2): 776-789.
・Ruiz-Lopez N, Haslam RP, Napier JA, Sayanova O (2014) Successful high-level accumulation of fish oil omega-3 long chain polyunsaturated fatty acids in a transgenic oilseed crop. Plant J. 77(2):198-208
・Rosenthal A, Pyle DL, and Niranjan K (1996) Aqueous and enzymatic processes for edible oil extraction. Enzyme and Microbial Technology 19:402-420.
Claims (13)
- オイルを含むオイル産生生物のバイオマスから、PUFAに富むオイルを抽出する方法であって、
当該方法が、
(a)前記オイル産生生物のバイオマスの細胞を溶解する工程と、
(b)溶解されたバイオマスからオイルを機械的に分離し、粗製オイルを回収する工程と
を含み、
前記細胞を溶解する工程(a)が、下記2つのパート:
(a1)第1の温度で実行される第1のパート、次いで
(a2)前記第1の温度よりも低い第2の温度で溶解を実行し続ける第2のパートを含むことを特徴とする、方法。 - 前記オイル産生生物のバイオマスが、PUFA産生微生物のバイオマスであることを特徴とする、請求項1に記載の方法。
- 前記バイオマスが、前記微生物の懸濁液であることを特徴とする、請求項2に記載の方法。
- 前記オイル産生生物によって産生されるオイルが50%超のPUFAを含むことを特徴とする、請求項1〜3のいずれか一項に記載の方法。
- 前記PUFAが、ドコサヘキサエン酸(DHA、C22:6n3)、ドコサペンタエン酸(DPA、C22:5n6)、アラキドン酸(ARA、C20:4n6)、エイコサペンタエン酸(EPA、C20:5n3)およびそれらの混合物から選択されるものであることを特徴とする、請求項1〜4のいずれか一項に記載の方法。
- 前記溶解工程(a)が、機械的溶解であることを特徴とする、請求項1〜5のいずれか一項に記載の方法。
- 前記溶解工程(a)が、酵素的溶解であることを特徴とする、請求項1〜5のいずれか一項に記載の方法。
- 前記工程(a2)の溶解終了時の温度が、前記第1のパート(a1)の温度よりも少なくとも10℃低いことを特徴とする、請求項1〜5のいずれか一項に記載の方法。
- 前記第2のパート(a2)の温度が、40℃以下であることを特徴とする、請求項8に記載の方法。
- 溶解されたバイオマスからのオイルの機械的分離(b)が、遠心プレートセパレータによる連続分離であることを特徴とする、請求項1〜9のいずれか一項に記載の方法。
- 前記工程(b)において得られた粗製オイルを精製する工程(c)を含むことを特徴とする、請求項1〜10のいずれか一項に記載の方法。
- 前記工程(b)において得られた粗製オイルまたは前記工程(c)において得られた精製されたオイルを希釈する工程(d)を含むことを特徴とする、請求項1〜11のいずれか一項に記載の方法。
- 請求項1〜12のいずれか一項に記載の方法によって得られたオイルを含むことを特徴とする、医薬組成物、化粧品組成物または食品組成物。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1858293A FR3085962B1 (fr) | 2018-09-14 | 2018-09-14 | Procede d'extracton d'une huile riche en pufa |
FR1858293 | 2018-09-14 | ||
PCT/EP2019/074461 WO2020053375A1 (fr) | 2018-09-14 | 2019-09-13 | Procede d'extraction d'une huile riche en acides gras polyunsatures (agpi) |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2022500049A true JP2022500049A (ja) | 2022-01-04 |
Family
ID=65201440
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2021514128A Pending JP2022500049A (ja) | 2018-09-14 | 2019-09-13 | 多価不飽和脂肪酸(pufa)に富むオイルの抽出方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US20220041953A1 (ja) |
EP (1) | EP3849329A1 (ja) |
JP (1) | JP2022500049A (ja) |
AU (1) | AU2019337405A1 (ja) |
FR (1) | FR3085962B1 (ja) |
WO (1) | WO2020053375A1 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR3111912A1 (fr) | 2020-06-24 | 2021-12-31 | Fermentalg | Procédé de culture de microorganismes pour l’accumulation de lipides |
FR3130842A1 (fr) | 2021-12-22 | 2023-06-23 | CarbonWorks | Procede de captation des phytotoxines dans un reacteur biologique |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003520046A (ja) * | 2000-01-19 | 2003-07-02 | マーテック バイオサイエンシズ ボールダーコーポレイション | 無溶媒抽出プロセス |
JP2006230403A (ja) * | 2005-01-31 | 2006-09-07 | Hokkaido Univ | ドコサヘキサエン酸(dha)の生産性の高い新規ラビリンチュラ類微生物およびその利用 |
JP2013532964A (ja) * | 2010-06-01 | 2013-08-22 | ディーエスエム アイピー アセッツ ビー.ブイ. | 細胞からの脂質の抽出およびそれに由来する生産物 |
JP2014500349A (ja) * | 2010-11-03 | 2014-01-09 | ソラザイム、インク | 流動点が低い微生物油、それから生成される誘電性流体、及び関連する方法 |
WO2014112574A1 (ja) * | 2013-01-18 | 2014-07-24 | 協和発酵バイオ株式会社 | ドコサヘキサエン酸を生産する微生物及びその利用 |
Family Cites Families (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR1186824A (fr) * | 1956-04-14 | 1959-09-02 | Procédé d'extraction des matières oléagineuses et produits conformes à ceux obtenus | |
JPH0716424B2 (ja) | 1985-10-01 | 1995-03-01 | ライオン株式会社 | アラキドン酸含有脂質の製造方法 |
US5340742A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
US5130242A (en) | 1988-09-07 | 1992-07-14 | Phycotech, Inc. | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
PH11992043811B1 (en) | 1991-01-24 | 2002-08-22 | Martek Corp | Arachidonic acid and methods for the production and use thereof |
DK0894142T4 (da) | 1996-03-28 | 2014-02-24 | Dsm Ip Assets Bv | Mikrobiel olie der omfatter en polyumættet fedtsyre og proces til produktion af olie fra pasteuriseret og granuleret biomasse. |
DK1785492T3 (da) | 1996-07-23 | 2010-07-26 | Nagase Chemtex Corp | Fremgangsmåde til fremstilling af docosahexaensyre og docosapentaensyre |
KR20090064603A (ko) | 2000-01-28 | 2009-06-19 | 마텍 바이오싸이언스스 코포레이션 | 발효기 내에서 진핵 미생물의 고밀도 배양에 의한 고도불포화 지방산을 함유하는 지질의 증진된 생산 방법 |
EP1178103A1 (en) | 2000-08-02 | 2002-02-06 | Dsm N.V. | Purifying crude pufa oils |
CA2469647C (en) | 2001-12-12 | 2011-02-15 | Daniel G. Dueppen | Extraction and winterization of lipids from oilseed and microbial sources |
NZ573719A (en) | 2006-07-05 | 2011-08-26 | Photonz Corp Ltd | Production of ultrapure eicosapentaenoic acid and polar lipids from largely heterotrophic culture of nitzschia laevis |
SE534278C2 (sv) | 2009-02-17 | 2011-06-28 | Alfa Laval Corp Ab | Ett kontinuerligt förfarande för isolering av oljor från alger eller mikroorganismer |
DK2408922T3 (en) | 2009-03-19 | 2016-12-05 | Dsm Ip Assets Bv | Thraustochytrids, FATTY ACID COMPOSITIONS AND METHODS OF MAKING AND USE THEREOF |
US8207363B2 (en) | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
CN101987765B (zh) | 2009-08-07 | 2012-06-27 | 无锡尚德太阳能电力有限公司 | 一种污水处理方法以及该处理方法所用的系统 |
FR2964667B1 (fr) | 2010-09-15 | 2014-08-22 | Fermentalg | Procede de culture d'algues unicellulaires mixotrophes en presence d'un apport lumineux discontinu sous forme de flashs |
PL2734626T3 (pl) * | 2011-07-21 | 2021-06-28 | Dsm Ip Assets B.V. | Oleje mikrobiologiczne wzbogacone w wielonienasycone kwasy tłuszczowe |
JP2014530614A (ja) * | 2011-10-20 | 2014-11-20 | フリンダーズユニバーシティ オブ サウス オーストラリアFlinders University of South Australia | 微細藻類の抽出 |
FR2988100B1 (fr) | 2012-03-16 | 2016-02-05 | Fermentalg | Production d'acide docosahexaenoique et d'astaxanthine en mode mixotrophe par schizochytrium |
FR2988098A1 (fr) | 2012-03-16 | 2013-09-20 | Fermentalg | Production d'acide docosahexaenoique en mode mixotrophe par nitzschia |
US9074160B2 (en) | 2013-03-07 | 2015-07-07 | Algisys, Llc | Production of omega-3 fatty acids from pythium species |
MX2015011767A (es) | 2013-03-15 | 2016-01-15 | Aurora Algae Inc | Composiciones de aceite crudo de algas. |
FR3008423B1 (fr) | 2013-07-12 | 2017-12-22 | Fermentalg | Nouvelle souche de aurantiochytrium |
FR3008422B1 (fr) | 2013-07-12 | 2017-11-17 | Fermentalg | Procede de culture cellulaire decouple |
PT3022290T (pt) | 2013-07-19 | 2018-04-02 | Fermentalg | Estirpe de algas modificadas e método de acumulação de triacilglicerol usando a referida estirpe |
ES2749002T3 (es) * | 2013-12-20 | 2020-03-18 | Mara Renewables Corp | Métodos de recuperación de aceite sobre microorganismos |
KR102426987B1 (ko) | 2013-12-20 | 2022-07-28 | 디에스엠 아이피 어셋츠 비.브이. | 미생물 세포로부터의 미생물 오일의 수득 방법 |
CN106029623A (zh) | 2013-12-20 | 2016-10-12 | 帝斯曼知识产权资产管理有限公司 | 用于从微生物细胞获得微生物油的方法 |
SG11201605009RA (en) | 2013-12-20 | 2016-07-28 | Dsm Ip Assets Bv | Processes for obtaining microbial oil from microbial cells |
FR3019559B1 (fr) | 2014-04-03 | 2018-01-05 | Fermentalg | Procede de culture des microalgues du genre aurantiochytrium dans un milieu de culture a teneur reduite en sodium et en chlorure pour la production de dha |
FR3025215A1 (fr) | 2014-08-27 | 2016-03-04 | Fermentalg | Nouveau procede de culture de microalgues |
ES2712298T3 (es) | 2014-10-15 | 2019-05-10 | Univ Santiago Compostela | Procedimiento de enriquecimiento de biomasa de microalgas en ácidos grasos poliinsaturados |
FR3038913B1 (fr) | 2015-07-17 | 2020-05-01 | Fermentalg | Biomasse de thraustochytrides, procede de culture et utilisations |
WO2017131188A1 (ja) | 2016-01-28 | 2017-08-03 | 日本水産株式会社 | 高度不飽和脂肪酸を含む油脂の製造方法 |
WO2018011275A1 (en) | 2016-07-13 | 2018-01-18 | Evonik Degussa Gmbh | Method for isolating lipids from lipid-containing cells |
-
2018
- 2018-09-14 FR FR1858293A patent/FR3085962B1/fr active Active
-
2019
- 2019-09-13 EP EP19765746.3A patent/EP3849329A1/fr active Pending
- 2019-09-13 US US17/275,848 patent/US20220041953A1/en active Pending
- 2019-09-13 WO PCT/EP2019/074461 patent/WO2020053375A1/fr unknown
- 2019-09-13 AU AU2019337405A patent/AU2019337405A1/en active Pending
- 2019-09-13 JP JP2021514128A patent/JP2022500049A/ja active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003520046A (ja) * | 2000-01-19 | 2003-07-02 | マーテック バイオサイエンシズ ボールダーコーポレイション | 無溶媒抽出プロセス |
JP2006230403A (ja) * | 2005-01-31 | 2006-09-07 | Hokkaido Univ | ドコサヘキサエン酸(dha)の生産性の高い新規ラビリンチュラ類微生物およびその利用 |
JP2013532964A (ja) * | 2010-06-01 | 2013-08-22 | ディーエスエム アイピー アセッツ ビー.ブイ. | 細胞からの脂質の抽出およびそれに由来する生産物 |
JP2014500349A (ja) * | 2010-11-03 | 2014-01-09 | ソラザイム、インク | 流動点が低い微生物油、それから生成される誘電性流体、及び関連する方法 |
WO2014112574A1 (ja) * | 2013-01-18 | 2014-07-24 | 協和発酵バイオ株式会社 | ドコサヘキサエン酸を生産する微生物及びその利用 |
Also Published As
Publication number | Publication date |
---|---|
EP3849329A1 (fr) | 2021-07-21 |
US20220041953A1 (en) | 2022-02-10 |
FR3085962B1 (fr) | 2021-06-18 |
WO2020053375A1 (fr) | 2020-03-19 |
FR3085962A1 (fr) | 2020-03-20 |
AU2019337405A1 (en) | 2021-05-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20200078464A1 (en) | Polyunsaturated fatty acid-containing solid fat compositions and uses and production thereof | |
KR100423013B1 (ko) | 도코사펜타엔산 함유물질의 이용 | |
KR101451415B1 (ko) | 불비누화 물질 함량을 저감시킨 미생물 유지의 제조 방법 및 그 유지 | |
DK1846563T3 (en) | PREPARATION OF POLYUM Saturated FAT ACIDS WITH USE OF THE UNDERTAKING PROCEDURE | |
EP2630869A1 (en) | Polyunsaturated fatty acid-containing oil product and uses and production thereof | |
TWI343418B (en) | Process for production of transesterified oils/fats or triglycerides | |
JP2008133286A (ja) | 神経学的障害の治療に有用な方法および医薬用組成物 | |
US8241868B2 (en) | Production of polyunsaturated fatty acids using cell treatment method | |
CN105925627B (zh) | 微生物油及其制备方法 | |
JP2022500049A (ja) | 多価不飽和脂肪酸(pufa)に富むオイルの抽出方法 | |
US20240141395A1 (en) | Oil of microorganisms rich in docosahexaenoic acid | |
WO2010087373A1 (ja) | アスタキサンチン含有ファフィア属酵母抽出物の製造法 | |
US20230235371A1 (en) | Method for culturing microorganisms for lipid accumulation | |
KR20050036012A (ko) | 장쇄고도불포화지방산을 함유하는 인지질조성물 및 이의 제조방법 | |
Bhosale et al. | Freshwater algae as potential source of polyunsaturated fatty acids |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20220803 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20230725 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20230801 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20231027 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20231225 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20240201 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20240402 |