JP2022130428A - 細胞培養システム及び方法 - Google Patents
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Abstract
Description
Claims (17)
- ドッキングステーション(104,105;204,205;704,705)、操作ユニット(102;202;302;402;702;802;902)、培養モジュール(101;201;301;401;701;801;901)及び作動層(120;220;320;420;720;820;920)を備える細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)であって、
培養モジュール(101;201;301;401;701;801;901)は培養ウェルを有し;
操作ユニット(102;202;302;402;702;802;902)は、培養モジュール(101;201;301;401;701;801;901)と作動層(120;220;320;420;720;820;920)とを収容するための台座(147)と、培養ウェルと連係される作動ボアを有する底部とを有し、底部は作動層(120;220;320;420;720;820;920)によって培養モジュール(101;201;301;401;701;801;901)から分離されており;且つ、
ドッキングステーション(104,105;204,205;704,705)は、操作ユニット(102;202;302;402;702;802;902)を所定の位置で取り外し可能に保持するための連結構造(146)と、作動供給チャネルとを有し、ここで操作ユニット(102;202;302;402;702;802;902)が連結構造(146)によって所定の位置で保持されると、作動供給チャネルの第1の端部が作動ボアに接続され、作動供給チャネルの第2の端部はコネクタ(149;734;834)に接続される、細胞培養システム。 - 培養モジュール(101;201;301;401;701;801;901)が、頂端培養チャンバ(122;222;322;422;722;822;922)及び基底培養チャンバ(123;223;323;423;723;823;923)内の培養ウェルを分離する培養膜(112;212;312;412;712;812;912)を有する、請求項1に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 操作ユニット(102;202;302;402;702;802;902)の台座(147)が培養モジュール(101;201;301;401;701;801;901)及び作動層(120;220;320;420;720;820;920)を取り外し可能に収容するために構成されており;且つ、
培養モジュール(101;201;301;401;701;801;901)が台座(147)内に配置されると、作動ボアが培養ウェルに連係され、底部が作動層(120;220;320;420;720;820;920)によって培養モジュール(101;201;301;401;701;801;901)から分離される、請求項1又は2に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。 - 培養モジュール(101;201;301;401;701;801;901)が入口ウェル(125;225;325;425;725;925)と出口ウェルとを有し;
操作ユニット(102;202;302;402;702;802;902)の底部が入口ボアと出口ボアとを有し、ここで培養モジュール(101;201;301;401;701;801;901)が台座(147)内に配置されると、入口ボアは入口ウェル(125;225;325;425;725;925)に連係され、出口ボアは出口ウェルに連係され;且つ、
ドッキングステーション(104,105;204,205;704,705)が入口供給チャネルと出口供給チャネルとを有し、ここで操作ユニット(102;202;302;402;702;802;902)が所定の位置で連結構造(146)によって保持されると、入口供給チャネルの第1の端部は入口ボアに接続され、出口供給チャネルの第1の端部は出口ボアに接続され、入口供給チャネル及び出口供給チャネルの各第2の端部はコネクタに接続される、請求項1から3のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。 - ポンプ装置を有する圧力制御ユニット(106,107;206,207)と、ポンプ装置に接続された少なくとも1つのポートと、少なくとも1つのポートのそれぞれにおいて圧力が個別に調整可能であるようにポンプ装置を制御するためのプロセッサとを備える、請求項1から4のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 細胞培養モジュール(101;201;301;401;701;801;901)が、培養モジュール(101;201;301;401;701;801;901)の上部に配置されたキャップ(931)を有し、キャップ(931)は、培養モジュール(101;201;301;401;701;801;901)及び操作ユニット(102;202;302;402;702;802;902)を介してドッキングステーション(104,105;204,205;704,705)に、さらに入口ウェル(125;225;325;425;725;925)に接続されたチャネル(930)を含む、請求項4又は5に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 細胞培養モジュール(101;201;301;401;701;801;901)のキャップ(931)が、培養モジュール(101;201;301;401;701;801;901)及び操作ユニット(102;202;302;402;702;802;902)を介してドッキングステーション(104,105;204,205;704,705)に、さらに出口ウェルに接続された第2のチャネルを有する、請求項6に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 細胞培養モジュール(101;201;301;401;701;801;901)が、培養ウェル上に配置された作動膜(637)を含む、請求項1から7のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 操作ユニット(102;202;302;402;702;802;902)と培養モジュール(101;201;301;401;701;801;901)との間に位置するチャネル(447)で互いに接続されている、第1の細胞型を含む第1の培養ウェルと第2の細胞型を含む第2の培養ウェルとを細胞培養モジュール(101;201;301;401;701;801;901)が有する、請求項1から8のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 追加の培養ウェルが相互接続される、請求項1から9のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 培養膜の頂端面又は培養膜の基底面で2つの培養ウェルを接続するチャネル(980;981)を備える、請求項2から10のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 培養膜がメッシュ(1690;1691;1692;1693)を含む、請求項2から11のいずれか一項に記載の細胞培養システム。
- バルブ(1360)を有するチャネル(1361)を備え、チャネル(1361)が出口ウェル(1326)と入口ウェル(1325)とを接続する、請求項4から12のいずれか一項に記載の細胞培養システム。
- ドッキングステーション(104,105;204,205;704,705)と同じさらなるドッキングステーション(104,105;204,205;704,705)と、細胞培養フードと、インキュベーションフードとを備え、ドッキングステーション(104,105;204,205;704,705)が培養フード内に配置され、さらなるドッキングステーション(104,105;204,205;704,705)がインキュベーションフード内に配置される、請求項1から13のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 操作ユニット(102;202;302;402;702;802;902)の底部の作動ボアが作動層(120;220;320;420;720;820;920)に近位のキャビティ部を有する、請求項1から14のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 培養モジュール(101;201;301;401;701;801;901)と構造的に同じ少なくとも1つのさらなる培養モジュール(101;201;301;401;701;801;901)を備え、ここで操作ユニット(102;202;302;402;702;802;902)が、さらなる培養モジュール(101;201;301;401;701;801;901)を収容するための少なくとも1つのさらなる台座(147)と、さらなる培養モジュール(101;201;301;401;701;801;901)の培養ウェルに連係されている少なくとも1つのさらなる作動ボアを有する底部とを有し;且つ、ドッキングステーション(104,105;204,205;704,705)はさらなる作動供給チャネルを有し、ここで操作ユニット(102;202;302;402;702;802;902)が連結構造(146)によって所定の位置で保持されると、さらなる作動供給チャネルの第1の端部がさらなる作動ボアに接続され、さらなる作動供給チャネルの第2の端部がコネクタ(149;734;834)に接続される、請求項1から15のいずれか一項に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
- 少なくとも1つのさらなる作動層(120;220;320;420;720;820;920)を備え、操作ユニットのさらなる台座(147)がさらなる作動層(120;220;320;420;720;820;920)を収容するように構成され、底部がさらなる作動層(120;220;320;420;720;820;920)によってさらなる培養モジュール(101;201;301;401;701;801;901)から分離される、請求項16に記載の細胞培養システム(100;200;300;400;600;700;800;900;1300;1400;1600)。
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