JP2022058674A - 試料観察装置及び試料観察方法 - Google Patents
試料観察装置及び試料観察方法 Download PDFInfo
- Publication number
- JP2022058674A JP2022058674A JP2022006859A JP2022006859A JP2022058674A JP 2022058674 A JP2022058674 A JP 2022058674A JP 2022006859 A JP2022006859 A JP 2022006859A JP 2022006859 A JP2022006859 A JP 2022006859A JP 2022058674 A JP2022058674 A JP 2022058674A
- Authority
- JP
- Japan
- Prior art keywords
- fluorescence
- image
- sample
- excitation light
- imaging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000005284 excitation Effects 0.000 claims abstract description 145
- 238000003384 imaging method Methods 0.000 claims abstract description 125
- 238000004458 analytical method Methods 0.000 claims abstract description 63
- 239000000126 substance Substances 0.000 claims abstract description 50
- 238000012545 processing Methods 0.000 claims abstract description 18
- 238000002073 fluorescence micrograph Methods 0.000 claims description 95
- 230000003287 optical effect Effects 0.000 claims description 83
- 239000000284 extract Substances 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 16
- 210000003855 cell nucleus Anatomy 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000002131 composite material Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Abstract
Description
Claims (12)
- 蛍光を用いて試料を観察する試料観察装置であって、
第1の蛍光物質によって染色された前記試料を第2の蛍光物質を含む溶液が注入されたウェル内に保持する試料容器と、
前記第1の蛍光物質を励起する第1の励起光、及び前記第2の蛍光物質を励起する第2の励起光を前記ウェルに向けて照射する照射部と、
前記試料容器を前記第1の励起光及び前記第2の励起光の光軸と交差する一方向に相対的に走査する走査部と、
前記第1の励起光によって前記試料で発生した第1の蛍光、及び前記第2の励起光によって前記溶液で発生した第2の蛍光をそれぞれ結像する結像部と、
前記結像部によって結像された前記第1の蛍光の光像の少なくとも一部を撮像して第1の画像データを出力し、前記結像部によって結像された前記第2の蛍光の光像の少なくとも一部を撮像して第2の画像データを出力する撮像部と、
前記一方向について得られた複数の前記第1の画像データに基づいて第1の蛍光画像を生成し、前記一方向について得られた複数の前記第2の画像データに基づいて第2の蛍光画像を生成する画像処理部と、
前記第2の蛍光画像に基づいて前記試料の存在領域を特定し、前記試料の存在領域に基づいて前記第1の蛍光画像における解析領域を設定し、前記解析領域における蛍光の強度を解析する解析部と、を備える試料観察装置。 - 前記結像部は、前記第1の蛍光を透過する第1の蛍光フィルタと、前記第2の蛍光を透過する第2の蛍光フィルタとを結像光路上で切り替える切替部を有する請求項1記載の試料観察装置。
- 前記結像部は、前記第1の蛍光と前記第2の蛍光とを分割する光分割素子を有し、
前記撮像部は、前記光分割素子によって分割された前記第1の蛍光の少なくとも一部を撮像する第1の光検出器と、前記光分割素子によって分割された前記第2の蛍光の少なくとも一部を撮像する第2の光検出器とを有する請求項1記載の試料観察装置。 - 前記結像部は、前記第1の蛍光と前記第2の蛍光とを分割する光分割素子を有し、
前記撮像部は、前記光分割素子によって分割された前記第1の蛍光の少なくとも一部を撮像する第1の撮像領域、及び前記光分割素子によって分割された前記第2の蛍光の少なくとも一部を撮像する第2の撮像領域を有する光検出器を備える請求項1記載の試料観察装置。 - 前記第1の励起光及び前記第2の励起光は、面状光である請求項1~4のいずれか一項記載の試料観察装置。
- 前記結像部は、前記第1の励起光及び前記第2の励起光の照射面に対して傾斜する観察軸を有する請求項5記載の試料観察装置。
- 前記解析部は、前記第2の蛍光画像を二値化することにより、前記試料の存在領域を抽出する請求項1~6のいずれか一項記載の試料観察装置。
- 前記第1の励起光の波長及び前記第2の励起光の波長は、異なる波長である請求項1~7記載のいずれか一項記載の試料観察装置。
- 前記第1の励起光の波長及び前記第2の励起光の波長は、同じ波長である請求項1~7記載のいずれか一項記載の試料観察装置。
- 前記第1の蛍光の波長及び前記第2の蛍光の波長は、異なる波長である請求項1~9記載のいずれか一項記載の試料観察装置。
- 前記第1の蛍光の波長及び前記第2の蛍光の波長は、同じ波長である請求項1~9記載のいずれか一項記載の試料観察装置。
- 蛍光を用いて試料を観察する試料観察方法であって、
第1の蛍光物質によって染色された前記試料を第2の蛍光物質を含む溶液が注入された試料容器のウェル内に保持する準備ステップと、
前記第1の蛍光物質を励起する第1の励起光、及び前記第2の蛍光物質を励起する第2の励起光を前記ウェルに向けて照射する照射ステップと、
前記試料容器を前記第1の励起光及び前記第2の励起光の光軸と交差する一方向に相対的に走査する走査ステップと、
前記第1の励起光によって前記試料で発生した第1の蛍光、及び前記第2の励起光によって前記溶液で発生した第2の蛍光をそれぞれ結像する結像ステップと、
前記結像ステップによって結像された前記第1の蛍光の光像の少なくとも一部を撮像して第1の画像データを出力し、前記結像ステップによって結像された前記第2の蛍光の光像の少なくとも一部を撮像して第2の画像データを出力する撮像ステップと、
前記一方向について得られた複数の前記第1の画像データに基づいて第1の蛍光画像を生成し、前記一方向について得られた複数の前記第2の画像データに基づいて第2の蛍光画像を生成する画像処理ステップと、
前記第2の蛍光画像に基づいて前記試料の存在領域を特定し、前記試料の存在領域に基づいて前記第1の蛍光画像における解析領域を設定し、前記解析領域における蛍光の強度を解析する解析ステップと、を備える試料観察方法。
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2017135426 | 2017-07-11 | ||
JP2017135426 | 2017-07-11 | ||
PCT/JP2018/015745 WO2019012771A1 (ja) | 2017-07-11 | 2018-04-16 | 試料観察装置及び試料観察方法 |
JP2019529451A JP7014794B2 (ja) | 2017-07-11 | 2018-04-16 | 試料観察装置及び試料観察方法 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2019529451A Division JP7014794B2 (ja) | 2017-07-11 | 2018-04-16 | 試料観察装置及び試料観察方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2022058674A true JP2022058674A (ja) | 2022-04-12 |
JP7329086B2 JP7329086B2 (ja) | 2023-08-17 |
Family
ID=65002001
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2019529451A Active JP7014794B2 (ja) | 2017-07-11 | 2018-04-16 | 試料観察装置及び試料観察方法 |
JP2022006859A Active JP7329086B2 (ja) | 2017-07-11 | 2022-01-20 | 試料観察装置及び試料観察方法 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2019529451A Active JP7014794B2 (ja) | 2017-07-11 | 2018-04-16 | 試料観察装置及び試料観察方法 |
Country Status (5)
Country | Link |
---|---|
US (2) | US11353402B2 (ja) |
EP (1) | EP3654020B1 (ja) |
JP (2) | JP7014794B2 (ja) |
CN (1) | CN110892251B (ja) |
WO (1) | WO2019012771A1 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102245077B1 (ko) * | 2021-01-11 | 2021-04-27 | 더죤환경기술(주) | 대기오염을 자동 정화하는 순환식 정화장치 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0381648A (ja) * | 1989-08-24 | 1991-04-08 | Olympus Optical Co Ltd | 細胞内イオンの2次元濃度分布像を形成する方法 |
JP2008523376A (ja) * | 2004-12-10 | 2008-07-03 | ジーイー・ヘルスケア・ユーケイ・リミテッド | 生体の画像化の方法、ならびにそのための装置およびコンピュータソフトウェア |
WO2013146843A1 (ja) * | 2012-03-30 | 2013-10-03 | コニカミノルタ株式会社 | 医用画像処理装置及びプログラム |
JP2015127738A (ja) * | 2013-12-27 | 2015-07-09 | ソニー株式会社 | 顕微鏡システムおよびその制御方法 |
WO2015118843A1 (ja) * | 2014-02-04 | 2015-08-13 | パナソニックIpマネジメント株式会社 | 試料検出プレート、これを用いた蛍光検出システム及び蛍光検出方法 |
JP2015537236A (ja) * | 2012-10-12 | 2015-12-24 | カール ツァイス マイクロスコピー ゲーエムベーハーCarl Zeiss Microscopy Gmbh | Spim顕微鏡法のための顕微鏡および方法 |
JP2016538584A (ja) * | 2013-10-09 | 2016-12-08 | ハワード ヒューズ メディカル インスティチュート | マルチビュー・ライトシート顕微鏡検査法 |
Family Cites Families (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5149972A (en) * | 1990-01-18 | 1992-09-22 | University Of Massachusetts Medical Center | Two excitation wavelength video imaging microscope |
US7071477B2 (en) * | 1994-07-15 | 2006-07-04 | Baer Stephen C | Superresolution in microlithography and fluorescence microscopy |
WO1998039636A1 (en) * | 1997-03-07 | 1998-09-11 | Clare Chemical Research Llc | Fluorometric detection using visible light |
US6905881B2 (en) * | 2000-11-30 | 2005-06-14 | Paul Sammak | Microbead-based test plates and test methods for fluorescence imaging systems |
US7186990B2 (en) * | 2002-01-22 | 2007-03-06 | Microbiosystems, Limited Partnership | Method and apparatus for detecting and imaging the presence of biological materials |
CA2525441A1 (en) * | 2003-05-13 | 2004-11-25 | Affymetrix, Inc. | System, method, and product for providing a wavelength-tunable excitation beam |
WO2005121862A1 (ja) * | 2004-06-14 | 2005-12-22 | Olympus Corporation | 光走査型顕微鏡観察装置 |
WO2006047038A1 (en) * | 2004-09-28 | 2006-05-04 | Trustees Of Tufts College | Apparatus and method for cell migration assays |
US7612350B2 (en) * | 2005-01-18 | 2009-11-03 | Olympus Corporation | Scanning microscope and specimen image obtaining method in which adjacent data obtaining points are not consecutively obtained |
JP5019866B2 (ja) * | 2006-12-25 | 2012-09-05 | オリンパス株式会社 | 蛍光内視鏡及び蛍光内視鏡の作動方法 |
JP2009111301A (ja) * | 2007-11-01 | 2009-05-21 | Hitachi High-Technologies Corp | プラズマ処理装置 |
GB0814039D0 (en) * | 2008-07-31 | 2008-09-10 | Imp Innovations Ltd | Optical arrangement for oblique plane microscopy |
JP5338573B2 (ja) * | 2009-08-31 | 2013-11-13 | ソニー株式会社 | 蛍光像取得方法、蛍光像取得装置及び蛍光像取得プログラム |
CN102667473B (zh) | 2009-10-12 | 2016-06-08 | 文塔纳医疗系统公司 | 用于组织的增强的病理学测定和多分析物检测的多模态对比和明场背景再现 |
US8673628B2 (en) * | 2011-06-10 | 2014-03-18 | Essen Instruments, Inc. | Methods and apparatus for improving in vitro measurements using boyden chambers |
WO2013185784A1 (en) * | 2012-06-11 | 2013-12-19 | Helmholtz Zentrum München Deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh) | Imaging system and method for imaging an object |
JP6086366B2 (ja) * | 2013-04-05 | 2017-03-01 | 国立研究開発法人理化学研究所 | 顕微鏡、焦準器具、流体保持器具、及び光学ユニット |
US10080484B2 (en) * | 2014-01-31 | 2018-09-25 | University Of Washington | Multispectral wide-field endoscopic imaging of fluorescence |
US11468557B2 (en) * | 2014-03-13 | 2022-10-11 | California Institute Of Technology | Free orientation fourier camera |
US10746981B2 (en) * | 2014-05-30 | 2020-08-18 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and devices for imaging large intact tissue samples |
AU2015327665B2 (en) * | 2014-09-29 | 2018-09-27 | Stryker European Operations Limited | Imaging a target fluorophore in a biological material in the presence of autofluorescence |
US10247672B2 (en) * | 2014-09-29 | 2019-04-02 | Howard Hughes Medical Institute | Non-linear structured illumination microscopy |
US10921255B2 (en) * | 2014-12-09 | 2021-02-16 | Bioaxial Sas | Optical measuring device and process |
CA2970053A1 (en) * | 2015-01-26 | 2016-08-04 | California Institute Of Technology | Multi-well fourier ptychographic and fluorescence imaging |
US10437038B2 (en) * | 2015-02-20 | 2019-10-08 | Max-Planck-Geselleschaft Zur Foerderung Der Wissenschaften E.V. | Device and method for creating an optical tomogram of a microscopic sample |
EP3088933A1 (en) * | 2015-05-01 | 2016-11-02 | Olympus Corporation | Microscope and microscope-image acquiring method |
US10605730B2 (en) * | 2015-05-20 | 2020-03-31 | Quantum-Si Incorporated | Optical sources for fluorescent lifetime analysis |
US11092795B2 (en) * | 2016-06-10 | 2021-08-17 | California Institute Of Technology | Systems and methods for coded-aperture-based correction of aberration obtained from Fourier ptychography |
JP2018004777A (ja) * | 2016-06-28 | 2018-01-11 | オリンパス株式会社 | 光シート顕微鏡、及び、光シート顕微鏡の制御方法 |
JP2018017970A (ja) * | 2016-07-29 | 2018-02-01 | オリンパス株式会社 | 光シート顕微鏡、及び、光シート顕微鏡の制御方法 |
JP7109423B2 (ja) * | 2016-08-15 | 2022-07-29 | ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツング | ライトシート顕微鏡 |
JP7026337B2 (ja) * | 2017-08-29 | 2022-02-28 | パナソニックIpマネジメント株式会社 | 光観測装置 |
US10754140B2 (en) * | 2017-11-03 | 2020-08-25 | California Institute Of Technology | Parallel imaging acquisition and restoration methods and systems |
JP2024004796A (ja) * | 2022-06-29 | 2024-01-17 | キヤノン株式会社 | 光電変換装置、光電変換システム |
-
2018
- 2018-04-16 US US16/629,220 patent/US11353402B2/en active Active
- 2018-04-16 EP EP18831515.4A patent/EP3654020B1/en active Active
- 2018-04-16 JP JP2019529451A patent/JP7014794B2/ja active Active
- 2018-04-16 CN CN201880045897.6A patent/CN110892251B/zh active Active
- 2018-04-16 WO PCT/JP2018/015745 patent/WO2019012771A1/ja unknown
-
2022
- 2022-01-20 JP JP2022006859A patent/JP7329086B2/ja active Active
- 2022-03-21 US US17/699,834 patent/US20220205913A1/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0381648A (ja) * | 1989-08-24 | 1991-04-08 | Olympus Optical Co Ltd | 細胞内イオンの2次元濃度分布像を形成する方法 |
JP2008523376A (ja) * | 2004-12-10 | 2008-07-03 | ジーイー・ヘルスケア・ユーケイ・リミテッド | 生体の画像化の方法、ならびにそのための装置およびコンピュータソフトウェア |
WO2013146843A1 (ja) * | 2012-03-30 | 2013-10-03 | コニカミノルタ株式会社 | 医用画像処理装置及びプログラム |
JP2015537236A (ja) * | 2012-10-12 | 2015-12-24 | カール ツァイス マイクロスコピー ゲーエムベーハーCarl Zeiss Microscopy Gmbh | Spim顕微鏡法のための顕微鏡および方法 |
JP2016538584A (ja) * | 2013-10-09 | 2016-12-08 | ハワード ヒューズ メディカル インスティチュート | マルチビュー・ライトシート顕微鏡検査法 |
JP2015127738A (ja) * | 2013-12-27 | 2015-07-09 | ソニー株式会社 | 顕微鏡システムおよびその制御方法 |
WO2015118843A1 (ja) * | 2014-02-04 | 2015-08-13 | パナソニックIpマネジメント株式会社 | 試料検出プレート、これを用いた蛍光検出システム及び蛍光検出方法 |
Also Published As
Publication number | Publication date |
---|---|
EP3654020A1 (en) | 2020-05-20 |
JP7329086B2 (ja) | 2023-08-17 |
EP3654020A4 (en) | 2021-04-07 |
WO2019012771A1 (ja) | 2019-01-17 |
US11353402B2 (en) | 2022-06-07 |
JPWO2019012771A1 (ja) | 2020-05-07 |
EP3654020B1 (en) | 2023-06-21 |
US20200132603A1 (en) | 2020-04-30 |
US20220205913A1 (en) | 2022-06-30 |
CN110892251B (zh) | 2023-09-15 |
JP7014794B2 (ja) | 2022-02-01 |
CN110892251A (zh) | 2020-03-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20230314782A1 (en) | Sample observation device and sample observation method | |
US11822066B2 (en) | Sample observation device and sample observation method | |
JP7329086B2 (ja) | 試料観察装置及び試料観察方法 | |
JP7007122B2 (ja) | 試料観察装置及び試料観察方法 | |
JP7125936B2 (ja) | 試料観察装置及び試料観察方法 | |
WO2021256429A1 (ja) | 試料観察装置及び試料観察方法 | |
US20240184090A1 (en) | Sample observation device and sample observation method | |
JP6978562B2 (ja) | 試料観察装置及び試料観察方法 | |
EP4321917A1 (en) | Sample observation device and sample observation method | |
WO2021246021A1 (ja) | 試料観察装置及び試料観察方法 | |
JP6754408B2 (ja) | 試料観察装置及び試料観察方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20220120 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20220131 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20230214 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230410 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20230509 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20230801 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20230804 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 7329086 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |