JP2022022504A - Composite material for mycorrhizal fungi growth-promoting vitalizer, compounding material and production method thereof, and coated seeds and production method thereof - Google Patents
Composite material for mycorrhizal fungi growth-promoting vitalizer, compounding material and production method thereof, and coated seeds and production method thereof Download PDFInfo
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
Description
本発明は、菌根菌生長促進活力剤用の複合材、菌根菌生長促進活力剤と菌根菌または菌根菌およびそのパートナー細菌との配合資材、並びにその配合資材の製造方法、その配合資材を用いたコーティング種子およびその製造方法に関するものである。 The present invention relates to a composite material for a mycorrhizal growth promoting stimulant, a compounding material of a mycorrhizal growth promoting stimulant and a mycorrhizal bacterium or mycorrhizal fungus and its partner bacterium, and a method for producing the compounding material, the compounding thereof. It relates to coated seeds using materials and a method for producing the same.
菌根菌の中でも最も古いアーバスキュラー菌根菌（ＡＭＦ）は、４億６千万年前から植物と「持ちつ持たれつ」の関係、つまり「共生」を作り上げて、植物の光合成産物を得る見返りに、植物の養水分吸収を促進したり、植物の病害虫抵抗性や環境ストレス耐性を付与したりする等、現在のほぼ全ての植物の生育に多大な貢献をしている。この菌根菌を活用することによって、化学合成農薬や化学肥料の不使用が可能となり、安心・安全で持続可能な有機作物栽培や有機環境緑化を実現できることが明らかになってきている。現在、菌根菌が市販化されているが、植物根への菌根菌の接種の効果をより安定化させる技術が必要となっている。 Arbuscular mycorrhizal fungi (AMF), the oldest of the mycorrhizal fungi, have created a relationship between plants and "holding and holding", that is, "symbiosis" for 460 million years, and obtain photosynthetic products of plants. In return, it promotes the absorption of nutrient water in plants and imparts pest resistance and environmental stress resistance to plants, making a great contribution to the growth of almost all plants today. By utilizing this mycorrhizal fungus, it has become possible to eliminate the use of chemically synthesized pesticides and fertilizers, and it has become clear that safe, secure and sustainable organic crop cultivation and organic environmental greening can be realized. Currently, mycorrhizal fungi are commercially available, but there is a need for a technique for further stabilizing the effect of inoculating mycorrhizal fungi on plant roots.
その改善策として、（１）菌根菌生長促進活力剤を活用して、菌根菌接種効果の安定性や持続性を高める必要がある。また、（２）この菌根菌生長促進活力剤と菌根菌または菌根菌およびそのパートナー細菌との配合資材を製造すると非常に効果的な菌根菌接種源となりうる。さらに、（３）菌根菌生長促進活力剤と菌根菌または菌根菌およびそのパートナー細菌との配合資材を用いた種子のコーティング方法は、菌根菌の活用場面が広がるという画期的な農業生産技術になる。 As the improvement measures, it is necessary to improve the stability and sustainability of the mycorrhizal inoculation effect by utilizing (1) mycorrhizal fungus growth promoting vital agent. Further, (2) If a compounding material of this mycorrhizal fungus growth promoting vital agent and mycorrhizal fungus or mycorrhizal fungus and its partner bacterium is produced, it can be a very effective mycorrhizal fungus inoculation source. Furthermore, (3) the seed coating method using a compounding material of a mycorrhizal fungus growth promoting vital agent and mycorrhizal fungi or mycorrhizal fungi and their partner bacteria is epoch-making in that the use of mycorrhizal fungi is expanded. Become an agricultural production technology.
現在までに明らかになっている菌根菌生長促進活力剤として、非特許文献１－３に示す物質が知られている。これら以外に、本発明では、株式会社フローラ製の植物活力剤ＨＢ１０１（商品名）も菌根菌生長促進活力剤であることを明らかにしている。図１は、これらの菌根菌生長促進活力剤をまとめたものである。 As a mycorrhizal fungus growth promoting vital agent that has been clarified so far, the substances shown in Non-Patent Documents 1-3 are known. In addition to these, in the present invention, it is clarified that the plant vitalizing agent HB101 (trade name) manufactured by Flora Co., Ltd. is also a mycorrhizal fungus growth promoting vitalizing agent. FIG. 1 summarizes these mycorrhizal fungi growth promoting vital agents.
しかし、これらの菌根菌生長促進活力剤は、水に不溶のもの、揮発しやすいもの等があり、安定的で効果の持続性を高めた菌根菌生長促進活力剤を含有する資材の製造にあたって考慮する必要がある。ただ、この不溶性や揮発性の菌根菌生長促進活力剤の場合には、本発明者の特許（特許文献１）にて示すように、これらをシクロデキストリンに包接することで問題解決できる方策が知られている。 However, some of these mycorrhizal fungi growth promoting activators are insoluble in water, those that are easily volatilized, etc. It is necessary to consider in doing so. However, in the case of this insoluble or volatile mycorrhizal fungus growth promoting vital agent, as shown in the patent of the present inventor (Patent Document 1), there is a measure that can solve the problem by encapsulating them with cyclodextrin. Are known.
パートナー細菌は、菌根菌の胞子内またはその周辺に生息する細菌であり、非特許文献４では、Ｂａｃｉｌｌｕｓ ｓｐ．（ＫＴＣＩＧＭＥ０１：ＮＢＲＣ１０９６３３、２０１３年５月８日受入）、Ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ（ＫＴＣＩＧＭＥ０２：ＮＢＲＣ１０９６３４、２０１３年５月８日受入）、Ｐａｅｎｉｂａｃｉｌｌｕｓ ｒｈｉｚｏｓｐｈａｅｒａｅ（ＫＴＣＩＧＭＥ０３：ＮＢＲＣ１０９６３５、２０１３年５月８日受入）およびＰｓｅｕｄｏｍｏｎａｓ ｓｐ．（ＫＣＩＧＣ０１：ＮＢＲＣ１０９６１３、２０１３年４月１２日受入）が同定されている。これらのパートナー細菌は、菌根菌の生長を促進するとともに、抗菌作用や殺虫作用を有し、窒素固定能やリン溶解能を有していることが明らかとなっている。
The partner bacterium is a bacterium that lives in or around the spores of Bacillus subtilis, and in Non-Patent
現在、菌根菌接種源が販売されているが、一部の接種源では接種効果が不安定なものがある。また、これまでに知られている菌根菌生長促進活力剤以外の新しい活力剤の発見も行う必要があるとともに、菌根菌に作用しやすいように水に可溶にしたり、揮発しにくくしたりした活力剤にしなければならない。さらに、菌根菌生長促進活力剤と菌根菌または菌根菌およびそのパートナー細菌とを配合した非常に有益な菌根菌接種源を製造する必要があるとともに、菌根菌の活用場面を拡げるためには、菌根菌生長促進活力剤と菌根菌または菌根菌およびそのパートナー細菌との配合資材を用いた画期的なコーティング種子の開発も極めて重要である。 Currently, mycorrhizal fungi inoculation sources are on the market, but some inoculation sources have unstable inoculation effects. In addition, it is necessary to discover new vital agents other than the previously known mycorrhizal fungi growth promoting vital agents, and at the same time, make them soluble in water so that they can easily act on mycorrhizal fungi, and make them difficult to volatilize. It must be a loose vitality agent. Furthermore, it is necessary to produce a highly beneficial mycorrhizal inoculation source containing a mycorrhizal fungus growth promoting vital agent and mycorrhizal fungi or mycorrhizal fungi and their partner bacteria, and to expand the utilization of mycorrhizal fungi. For this purpose, it is also extremely important to develop epoch-making coated seeds using a compounding material of a mycorrhizal fungus growth promoting vital agent and mycorrhizal fungi or mycorrhizal fungi and their partner bacteria.
このように、菌根菌の普及を図るためには、現状の単なる菌根菌のみの接種源だけでなく、安定的な接種効果を示し、効果が持続する菌根菌接種源を作製する必要がある。そして、この技術を活用して、菌根菌やパートナー細菌を積極的に活用できる、極めてユニークなコーティング種子を開発しなければならない。 In this way, in order to promote the spread of mycorrhizal fungi, it is necessary to prepare not only the current inoculation source of mycorrhizal fungi but also mycorrhizal fungus inoculation sources that show stable inoculation effect and maintain the effect. There is. And we must utilize this technology to develop extremely unique coated seeds that can actively utilize mycorrhizal fungi and partner bacteria.
本発明は、上述した問題点を解決するためになされたものであり、安定性および持続性を高めることができる菌根菌生長促進活力剤用の複合材、その複合材を配合した配合資材、その製造方法、その配合資材を用いたコーティング種子およびその製造方法を提供することを目的としている。 The present invention has been made to solve the above-mentioned problems, and is a composite material for a mycorrhizal fungus growth promoting vital agent capable of enhancing stability and sustainability, a compounding material containing the composite material, and the like. It is an object of the present invention to provide the production method, coated seeds using the compounding material, and the production method thereof.
上記の目的を達成するために、本発明の菌根菌生長促進活力剤用の複合材は、菌根菌生長促進活力剤を、ゼオライト、炭、コーヒー粕、珪藻土、ベントナイト、およびモンモリロナイトの少なくともいずれか１つを含む資材に含有させた、ことを特徴としている。 In order to achieve the above object, the composite material for the mycorrhizal fungus growth promoting activator of the present invention comprises the mycorrhizal fungus growth promoting activator at least one of zeolite, charcoal, coffee grounds, diatomaceous earth, bentonite, and montmorillonite. It is characterized by being contained in a material containing one of them.
そして、水に不溶性および揮発性の少なくとも何れかの性質を有する前記菌根菌生長促進活力剤は、シクロデキストリンで包接させてから、前記資材に含有させる、ことが好ましい。 Then, it is preferable that the mycorrhizal fungus growth promoting vital agent having at least one of insoluble and volatile properties in water is encapsulated with cyclodextrin and then contained in the material.
また、本発明の配合資材は、上記の複合材と、菌根菌と、を配合したことを特徴としている。 Further, the compounding material of the present invention is characterized in that the above-mentioned composite material and mycorrhizal fungi are compounded.
そして、上記の配合資材は、菌根菌の胞子内またはその周辺に生息する微生物であるパートナー細菌を配合した、ことが好ましい。 The above-mentioned compounding material preferably contains a partner bacterium, which is a microorganism that lives in or around the spores of mycorrhizal fungi.
さらに、上記の配合資材は、前記パートナー細菌は、Ｂａｃｉｌｌｕｓ属における、Ｂａｃｉｌｌｕｓ ｓｐ．（ＫＴＣＩＧＭＥ０１）ＮＢＲＣ１０９６３３菌株、Ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ（ＫＴＣＩＧＭＥ０２）ＮＢＲＣ１０９６３４菌株、Ｐａｅｎｉｂａｃｉｌｌｕｓ属における、Ｐａｅｎｉｂａｃｉｌｌｕｓ ｒｈｉｚｏｓｐｈａｅｒａｅ（ＫＴＣＩＧＭＥ０３）ＮＢＲＣ１０９６３５菌株、およびＰｓｅｕｄｏｍｏｎａｓ属における、Ｐｓｅｕｄｏｍｏｎａｓ ｓｐ．（ＫＣＩＧＣ０１）ＮＢＲＣ１０９６１３菌株、の群より選択された少なくとも一種類以上の微生物を含む、ことが好ましい。 Further, in the above compounding material, the partner bacterium is Bacillus sp. In the genus Bacillus. (KTCIGME01) NBRC109633 strain, Bacillus thuringiensis (KTCIGME02) NBRC109634 strain, Paenibacillus rhizosphaerae (KTCIGME03) NBRC109635 strain, and Pseudomonas. It preferably contains at least one or more microorganisms selected from the group (KCIGC01) NBRC109613 strain.
本発明の配合資材の製造方法は、上記の配合資材の製造方法であって、前記菌根菌生長促進活力剤を前記資材に含ませる工程と、前記菌根菌を前記資材に生息させる工程と、を有することを特徴としている。 The method for producing a compounding material of the present invention is the above-mentioned method for producing a compounding material, which comprises a step of incorporating the mycorrhizal fungus growth-promoting vital agent into the material and a step of inhabiting the mycorrhizal fungus in the material. , Is characterized by having.
また、本発明のコーティング種子は、上記の配合資材を種子にコーティングさせた、ことを特徴としている。 Further, the coated seed of the present invention is characterized in that the seed is coated with the above-mentioned compounding material.
さらに、本発明のコーティング種子の製造方法は、上記のコーティング種子の製造方法であって、前記菌根菌生長促進活力剤を前記資材に含ませる工程と、前記菌根菌を前記種子周辺に生息させる工程と、を備えたことを特徴としている。 Further, the method for producing a coated seed of the present invention is the above-mentioned method for producing a coated seed, in which the step of incorporating the mycorrhizal fungus growth promoting vital agent into the material and the mycorrhizal fungus inhabiting the vicinity of the seed. It is characterized by having a process of making it.
本発明によれば、菌根菌生長促進活力剤の安定性や持続性を高めることができ、この菌根菌生長促進活力剤と菌根菌または菌根菌およびそのパートナー細菌との配合資材の製造や、その配合資材を用いたコーティング種子を製造できる。 According to the present invention, the stability and sustainability of the mycorrhizal fungus growth promoting vital agent can be enhanced, and the compounding material of this mycorrhizal fungus growth promoting vital agent and mycorrhizal fungi or mycorrhizal fungi and its partner bacteria. It can be produced and coated seeds using the compounding materials can be produced.
本発明によって、菌根菌の働きが活性化するので、作物の養水分吸収が早く活発になるだけでなく、作物の病虫害抵抗性や環境ストレス耐性も高まりやすくなる。また、パートナー細菌は、菌根菌の生長を促進するだけでなく、抗菌作用および殺虫作用を有し、大気中の窒素を固定でき、また、土壌中の難溶性あるいは不溶性のリンを溶解できる力を持つ微生物であるので、本発明の配合資材で菌根菌と共働して、窒素やリンの施用量を削減しても作物の生長を旺盛にさせうることが期待される。また、この配合資材を用いたコーティング種子は、菌根菌やパートナー細菌の活用場面を著しく拡大させる技術になると考えられる。 According to the present invention, since the action of mycorrhizal fungi is activated, not only the nutrient water absorption of the crop becomes active quickly, but also the pest resistance and the environmental stress resistance of the crop are likely to increase. In addition, partner bacteria not only promote the growth of mycorrhizal fungi, but also have antibacterial and insecticidal effects, can fix nitrogen in the air, and can dissolve sparingly soluble or insoluble phosphorus in soil. It is expected that the compounding material of the present invention can cooperate with mycorrhizal fungi to increase the growth of crops even if the application rate of nitrogen or phosphorus is reduced. In addition, coated seeds using this compounding material are considered to be a technology that significantly expands the utilization of mycorrhizal fungi and partner bacteria.
このように、本発明による安定性や持続性を高めた菌根菌生長促進活力剤と菌根菌または菌根菌およびそのパートナー細菌との配合資材は、作物の養水分吸収を促進し、かつ作物に病害虫抵抗性や環境ストレス耐性を付与して、作物の生長を旺盛にするとともに、収量の増加や作物の品質向上を図ることができる。 As described above, the compounding material of the mycorrhizal fungus growth promoting vital agent having enhanced stability and sustainability according to the present invention and the mycorrhizal fungus or mycorrhizal fungus and its partner bacteria promotes the absorption of nutrient water in the crop and By imparting pest resistance and environmental stress resistance to crops, it is possible to increase the growth of crops, increase the yield, and improve the quality of crops.
本発明によれば、菌根菌生長促進活力剤の安定性や持続性を高めることができる。 According to the present invention, the stability and sustainability of the mycorrhizal fungus growth promoting vital agent can be enhanced.
以下、本発明の好ましい実施形態について説明する。 Hereinafter, preferred embodiments of the present invention will be described.
（１）菌根菌生長促進活力剤の製造方法
菌根菌生長促進活力剤の中には、水に不溶のもの、揮発しやすいもの等があり、安定的で効果の持続性を高めた菌根菌生長促進活力剤が必要である。そこで、特許文献１に記載の方法にて菌根菌生長促進活力剤にシクロデキストリンで包接させたものを作製した。なお、水溶性および不揮発性の少なくとも何れかの性質を有する菌根菌生長促進活力剤は、シクロデキストリンで包接させることなく、そのままの状態で良い。
(1) Manufacturing method of mycorrhizal fungus growth promoting vital agent Some mycorrhizal fungal growth promoting vital agents are insoluble in water, easily volatilized, etc., and are stable and have enhanced long-lasting effects. Root fungus growth promoting vitalizer is required. Therefore, by the method described in
（２）新規の菌根菌生長促進活力剤とその実施例
植物活力剤ＨＢ１０１（商品名：株式会社フローラ製）が菌根菌（Ｇｌｏｍｕｓ ｃｌａｒｕｍ）の生長に及ぼす影響を調査した。図２に示すように、ＨＢ１０１の各濃度下における菌根菌の菌糸生長を２５℃の暗黒下で調査した。その結果、１０－４希釈液で菌糸生長が著しく促進される傾向がみられた（図２および図３）。このことは、ＨＢ１０１のような活力剤の植物への生長促進効果が菌根菌とそのパートナー細菌の存在下で顕著になることを示唆している。
(2) A novel mycorrhizal fungus growth promoting vital agent and its example The effect of the plant vital agent HB101 (trade name: manufactured by Flora Co., Ltd.) on the growth of mycorrhizal fungi (Glomus clarum) was investigated. As shown in FIG. 2, the hyphal growth of mycorrhizal fungi under each concentration of HB101 was investigated in the dark at 25 ° C. As a result, the hyphal growth tended to be significantly promoted with the 10-4 diluted solution (FIGS. 2 and 3). This suggests that the growth promoting effect of a vital agent such as HB101 on plants becomes remarkable in the presence of mycorrhizal fungi and their partner bacteria.
（３）菌根菌生長促進活力剤を含有させた複合材、例えば菌根菌生長促進活力剤と、菌根菌または菌根菌およびそのパートナー細菌との配合資材の製造方法
前記菌根菌生長促進活力剤をゼオライト、炭、コーヒー粕のような資材と混合させて菌根菌生長促進活力剤を含有させた複合材を作製する。そして、この複合材に、菌根菌または菌根菌およびそのパートナー細菌を配合させる。菌根菌は、Ｇｌｏｍｕｓ ｃｌａｒｕｍのようなアーバスキュラー菌根菌とともに、エリコイド菌根菌、ラン型菌根菌、外生菌根菌等も用いることができる。また、パートナー細菌は、菌根菌の胞子内またはその周辺に生息する細菌で、次の群より選択された一種類以上の微生物である。
(3) Method for producing a composite material containing a mycorrhizal fungus growth promoting vital agent, for example, a compound material of a mycorrhizal fungus growth promoting vital agent and a mycorrhizal fungus or mycorrhizal fungus and its partner bacteria. A composite material containing a mycorrhizal fungus growth promoting vital agent is prepared by mixing the promoting vitalizing agent with materials such as zeolite, charcoal, and coffee grounds. Then, mycorrhizal fungi or mycorrhizal fungi and their partner bacteria are blended into this composite material. As the mycorrhizal fungus, not only arbuscular mycorrhizal fungi such as Glomus clarum, but also Ericoid mycorrhiza, orchid-type mycorrhizal fungi, ectomycorrhizal fungi and the like can be used. The partner bacterium is a bacterium that lives in or around the spores of mycorrhizal fungi, and is one or more types of microorganisms selected from the following groups.
１）Ｂａｃｉｌｌｕｓ ｓｐ．（ＫＴＣＩＧＭＥ０１）ＮＢＲＣ１０９６３３菌株
２）Ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ（ＫＴＣＩＧＭＥ０２）ＮＢＲＣ１０９６３４菌株
３）Ｐａｅｎｉｂａｃｉｌｌｕｓ ｒｈｉｚｏｓｐｈａｅｒａｅ（ＫＴＣＩＧＭＥ０３）ＮＢＲＣ１０９６３５菌株
４）Ｐｓｅｕｄｏｍｏｎａｓ ｓｐ．（ＫＣＩＧＣ０１）ＮＢＲＣ１０９６１３菌株
なお、相同性が９７％以上のものを含む。菌根菌生長促進活力剤を含有させた資材、菌根菌、並びにパートナー細菌の配合割合は、作物の種類、栽培環境等によって変化すると考えられるので、その都度、適切な配合割合を決定する。
1) Bacillus sp. (KTCIGME01) NBRC109633 strain 2) Bacillus thuringiensis (KTCIGME02) NBRC109634 strain 3) Paenibacillus rhizosphaerae (KTCIGME03) NBRC109635 strain 4) Pseudomonas (KCIGC01) NBRC109613 strain In addition, those having a homology of 97% or more are included. Since it is considered that the blending ratio of the material containing the mycorrhizal fungus growth promoting vital agent, the mycorrhizal fungus, and the partner bacterium varies depending on the type of crop, the cultivation environment, etc., an appropriate blending ratio is determined each time.
（３－１）実験例
上記の菌根菌生長促進活力剤を含有させたゼオライトと、菌根菌と、そのパートナー細菌との配合資材がトマトの生長に及ぼす影響の実験例について説明する。
(3-1) Experimental Example An experimental example of the effect of the zeolite containing the above-mentioned mycorrhizal fungus growth promoting vital agent, the mycorrhizal fungus, and the compounding material of the partner bacterium on the growth of tomato will be described.
（実験）
トマト（品種：オレンジアイコ）の種子を培土に播種した後、無加温のビニルハウスで養成した。発芽後、菌根菌生長促進活力剤を含有させたゼオライトと、菌根菌と、そのパートナー細菌との配合資材２ｇを施用した。対照区は、菌根菌生長促進活力剤を含有させたゼオライトのみとした。なお、菌根菌生長促進活力剤にはＨＢ１０１（商品名）を、菌根菌にはＧｌｏｍｕｓ ｃｌａｒｕｍというアーバスキュラー菌根菌を、パートナー細菌には上記の細菌４種類を供試した。処理開始２週間後、苗の生育を観察した。
(experiment)
After sowing tomato (variety: orange aiko) seeds in the soil, they were cultivated in an unheated greenhouse. After germination, 2 g of a compounding material of zeolite containing a mycorrhizal fungus growth promoting vital agent, mycorrhizal fungi and its partner bacteria was applied. The control group was only zeolite containing a mycorrhizal growth promoting vital agent. HB101 (trade name) was used as a mycorrhizal growth promoting activator, an arbuscular mycorrhizal fungus called Glomus clarum was used as a mycorrhizal fungus, and the above four types of bacteria were used as partner bacteria. Two weeks after the start of treatment, the growth of seedlings was observed.
その結果、菌根菌生長促進活力剤を含有させたゼオライトと、菌根菌と、そのパートナー細菌との配合資材を用いた区（処理区）の苗の生長は、処理開始わずか２週間後でも、対照区と比べて著しく旺盛に生長することが認められた。なお、図４は、菌根菌生長促進活力剤（ＨＢ１０１）を含有させたゼオライトと、菌根菌と、そのパートナー細菌との配合資材がトマトの生長に及ぼす影響を示す図である。 As a result, the growth of seedlings in the plot (treatment plot) using the zeolite containing the mycorrhizal fungus growth promoting vital agent and the compounding material of the mycorrhizal fungus and its partner bacteria can be achieved only 2 weeks after the start of treatment. , It was found that the growth was remarkably vigorous compared to the control plot. FIG. 4 is a diagram showing the effect of the compounding material of zeolite containing a mycorrhizal fungus growth promoting vital agent (HB101), mycorrhizal fungi, and its partner bacteria on the growth of tomatoes.
（４）菌根菌生長促進活力剤を含有させた資材と、菌根菌または菌根菌およびそのパートナー細菌との配合資材を用いたコーティング種子の製造方法
菌根菌の胞子や菌糸体を含む前記菌根菌生長促進活力剤を含有させたゼオライト３０ｇを、上記パートナー細菌を含む１％から３％のアルギン酸ナトリウム水溶液１００ｍｌ程度の割合で配合する。その後、この配合液中に作物種子を浸漬するか、配合液を種子に塗布させた後、１％塩化カルシウム溶液に滴下摘果してゲル化させる。これを室温下等で２４時間程度乾燥させて造粒して、菌根菌生長促進活力剤を含有し菌根菌とそのパートナー細菌との配合資材が種子の表面にコーティングされたコーティング種子を製造する。
(4) Method for producing coated seeds using a material containing a mycorrhizal growth promoting vital agent and a compounding material of mycorrhizal fungi or mycorrhizal fungi and their partner bacteria, including mycelium spores and mycelia. 30 g of zeolite containing the mycelial growth promoting vital agent is blended at a ratio of about 100 ml of a 1% to 3% sodium alginate aqueous solution containing the partner bacteria. Then, the crop seeds are dipped in this compounding solution, or the compounding solution is applied to the seeds, and then the seeds are dropped and plucked in a 1% calcium chloride solution to gel them. This is dried at room temperature for about 24 hours to granulate to produce coated seeds containing a mycorrhizal fungus growth promoting vital agent and a compounding material of mycorrhizal fungi and their partner bacteria coated on the surface of the seeds. do.
ゼオライト以外に、炭、コーヒー粕、珪藻土、ベントナイト、モンモリロナイト等の資材も活用できるが、ゼオライトが最も適している。また、アルギン酸ナトリウム以外にも、カルボキシメチルセルロースナトリウムや、ポリアクリル酸ナトリウムも活用できるが、ポリアクリル酸ナトリウムは、土壌で分解しにくいため、使用が好ましくない。 In addition to zeolite, materials such as charcoal, coffee grounds, diatomaceous earth, bentonite, and montmorillonite can be used, but zeolite is the most suitable. In addition to sodium alginate, sodium carboxymethyl cellulose and sodium polyacrylate can also be used, but sodium polyacrylate is not preferable because it is difficult to decompose in soil.
以上、本発明の特長や製造方法について説明したが、本発明は上記の特長や実施形態に何ら限定されるものではなく、本発明の趣旨を逸脱しない範囲内で種々の改良変形が可能であることは容易に推察できるものである。 Although the features and manufacturing methods of the present invention have been described above, the present invention is not limited to the above features and embodiments, and various improvements and modifications can be made without departing from the spirit of the present invention. That is easy to infer.
例えば、図１は、現在までに見出された菌根菌生長促進活力剤であるが、今後、新規の菌根菌生長促進活力剤も発見されると考えられ、本発明は、これら新規の菌根菌生長促進活力剤にも対応できる製造方法である。 For example, FIG. 1 shows the mycorrhizal fungi growth-promoting vital agents found so far, but it is considered that new mycorrhizal fungal growth-promoting vital agents will be discovered in the future, and the present invention is the present invention. It is a manufacturing method that can be applied to a mycorrhizal fungus growth promoting vital agent.
また、上記実施形態の３－１では、上記の４種のパートナー細菌すべてを供試したが、必ずしもこれらの細菌に限られず、他の１又は複数の種類の有益微生物をさらに加えても良い。他の有益な微生物としては、例えば、乳酸菌、酵母、ボーベリア菌等がある。上記の４種類のパートナー細菌は、いずれも乳酸菌、酵母、ボーベリア菌等と共存できる微生物であるため、さらに有効（安定した良質）な菌根菌生長促進活力剤を含有させた複合材と、菌根菌あるいは菌根菌とそのパートナー細菌とを配合した配合資材を製造できる。 Further, in 3-1 of the above embodiment, all of the above four kinds of partner bacteria were tested, but the test is not limited to these bacteria, and another one or more kinds of beneficial microorganisms may be further added. Other beneficial microorganisms include, for example, lactic acid bacteria, yeast, Beauveria and the like. Since all of the above four types of partner bacteria are microorganisms that can coexist with lactic acid bacteria, yeast, Beauveria bacteria, etc., a composite material containing a more effective (stable and high-quality) mycorrhizal fungus growth promoting vital agent and bacteria. It is possible to manufacture a compounding material containing a root bacterium or a mycorrhizal bacterium and its partner bacterium.
Claims (8)
ゼオライト、炭、コーヒー粕、珪藻土、ベントナイト、およびモンモリロナイトの少なくともいずれか１つを含む資材に含有させた、
ことを特徴とする菌根菌生長促進活力剤用の複合材。 Mycorrhizal fungus growth promoting vital agent,
Ingredients containing at least one of zeolite, charcoal, coffee grounds, diatomaceous earth, bentonite, and montmorillonite,
A composite material for a mycorrhizal fungus growth promoting vital agent.
ことを特徴とする請求項１に記載の菌根菌生長促進活力剤用の複合材。 The mycorrhizal fungus growth-promoting vital agent having at least one of water-insoluble and volatile properties was encapsulated with cyclodextrin and then contained in the material.
The composite material for a mycorrhizal fungus growth promoting vital agent according to claim 1.
菌根菌と、
を配合したことを特徴とする配合資材。 With the composite material according to claim 1 or 2.
Mycorrhizal fungi and
A compounding material characterized by blending.
ことを特徴とする請求項３に記載の配合資材。 The compounding material according to claim 3, wherein a partner bacterium, which is a microorganism living in or around the spores of mycorrhizal fungi, is compounded.
Ｂａｃｉｌｌｕｓ属における、Ｂａｃｉｌｌｕｓ ｓｐ．（ＫＴＣＩＧＭＥ０１）ＮＢＲＣ１０９６３３菌株、Ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ（ＫＴＣＩＧＭＥ０２）ＮＢＲＣ１０９６３４菌株、
Ｐａｅｎｉｂａｃｉｌｌｕｓ属における、Ｐａｅｎｉｂａｃｉｌｌｕｓ ｒｈｉｚｏｓｐｈａｅｒａｅ（ＫＴＣＩＧＭＥ０３）ＮＢＲＣ１０９６３５菌株、および
Ｐｓｅｕｄｏｍｏｎａｓ属における、Ｐｓｅｕｄｏｍｏｎａｓ ｓｐ．（ＫＣＩＧＣ０１）ＮＢＲＣ１０９６１３菌株、の群より選択された少なくとも一種類以上の微生物を含む、
ことを特徴とする請求項４に記載の配合資材。 The partner bacterium is
Bacillus sp. In the genus Bacillus. (KTCIGME01) NBRC109633 strain, Bacillus thuringiensis (KTCIGME02) NBRC109634 strain,
Paenibacillus rhizosophaerae (KTCIGME03) NBRC109635 strain in the genus Paenibacillus, and Pseudomonas sp. In the genus Pseudomonas. (KCIGC01) NBRC109613 strain, which comprises at least one type of microorganism selected from the group.
The compounding material according to claim 4, wherein the compounding material is characterized by the above.
前記菌根菌生長促進活力剤を前記資材に含ませる工程と、
前記菌根菌を前記資材に生息させる工程と、
を有することを特徴とする配合資材の製造方法。 The method for manufacturing a compounding material according to any one of claims 3 to 5.
The step of incorporating the mycorrhizal fungus growth promoting vital agent into the material, and
The process of inhabiting the mycorrhizal fungus in the material and
A method for manufacturing a compounding material, which comprises.
ことを特徴とするコーティング種子。 The seeds were coated with the compounding material according to any one of claims 3 to 5.
It is characterized by coated seeds.
前記菌根菌生長促進活力剤を前記資材に含ませる工程と、
前記菌根菌を前記種子周辺に生息させる工程と、
を備えたことを特徴とするコーティング種子の製造方法。 The method for producing coated seeds according to claim 7.
The step of incorporating the mycorrhizal fungus growth promoting vital agent into the material, and
The step of inhabiting the mycorrhizal fungus around the seed and
A method for producing coated seeds, which is characterized by being provided with.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05153966A (en) * | 1991-11-29 | 1993-06-22 | Idemitsu Kosan Co Ltd | Proliferation of va mycorrhizal bacteria |
| JPH0823963A (en) * | 1994-07-21 | 1996-01-30 | Idemitsu Kosan Co Ltd | Method for culturing plant |
| JPH09233913A (en) * | 1996-03-01 | 1997-09-09 | Toko Kensetsu Kk | Coated seeds, production of the same and greening construction using the same |
| JP2001061344A (en) * | 1999-08-26 | 2001-03-13 | Gun Ei Chem Ind Co Ltd | Plant-vitalizing agent and crop-cultivating method using the plant-vitalizing agent |
| WO2015041336A1 (en) * | 2013-09-19 | 2015-03-26 | 京都府公立大学法人 | Hydroponic method utilizing beneficial micro-organisms |
| JP2019064890A (en) * | 2017-10-04 | 2019-04-25 | 孝昭 石井 | Organic liquid fertilizer and organic solid fertilizer, and method of producing organic liquid fertilizer and organic solid fertilizer |
| WO2019078209A1 (en) * | 2017-10-17 | 2019-04-25 | 花王株式会社 | Plant growth-promoting agent |
-
2020
- 2020-06-24 JP JP2020109106A patent/JP2022022504A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05153966A (en) * | 1991-11-29 | 1993-06-22 | Idemitsu Kosan Co Ltd | Proliferation of va mycorrhizal bacteria |
| JPH0823963A (en) * | 1994-07-21 | 1996-01-30 | Idemitsu Kosan Co Ltd | Method for culturing plant |
| JPH09233913A (en) * | 1996-03-01 | 1997-09-09 | Toko Kensetsu Kk | Coated seeds, production of the same and greening construction using the same |
| JP2001061344A (en) * | 1999-08-26 | 2001-03-13 | Gun Ei Chem Ind Co Ltd | Plant-vitalizing agent and crop-cultivating method using the plant-vitalizing agent |
| WO2015041336A1 (en) * | 2013-09-19 | 2015-03-26 | 京都府公立大学法人 | Hydroponic method utilizing beneficial micro-organisms |
| JP2019064890A (en) * | 2017-10-04 | 2019-04-25 | 孝昭 石井 | Organic liquid fertilizer and organic solid fertilizer, and method of producing organic liquid fertilizer and organic solid fertilizer |
| WO2019078209A1 (en) * | 2017-10-17 | 2019-04-25 | 花王株式会社 | Plant growth-promoting agent |
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