JP2021525105A - 標的細菌を死滅させるための方法および組成物 - Google Patents

標的細菌を死滅させるための方法および組成物 Download PDF

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Publication number
JP2021525105A
JP2021525105A JP2021516552A JP2021516552A JP2021525105A JP 2021525105 A JP2021525105 A JP 2021525105A JP 2021516552 A JP2021516552 A JP 2021516552A JP 2021516552 A JP2021516552 A JP 2021516552A JP 2021525105 A JP2021525105 A JP 2021525105A
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Prior art keywords
bacteriophage
crispr
target
gene
essential
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Japanese (ja)
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JP2021525105A5 (https=
Inventor
エム． ガロフォロ，ポール
エム． ガロフォロ，ポール
ジー． オースターアウト，デビッド
ジー． オースターアウト，デビッド
セル，カート
ウォン，サンディ
ローレンス ベイセル，チェイス
ローレンス ベイセル，チェイス
クマー シン，アチュル
クマー シン，アチュル
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North Carolina State University
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North Carolina State University
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Publication of JP2021525105A publication Critical patent/JP2021525105A/ja
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40—Viruses, e.g. bacteriophages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/76—Viruses; Subviral particles; Bacteriophages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14033—Use of viral protein as therapeutic agent other than vaccine, e.g. apoptosis inducing or anti-inflammatory
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Agronomy & Crop Science (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2021516552A 2018-05-25 2019-05-24 標的細菌を死滅させるための方法および組成物 Pending JP2021525105A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862676818P 2018-05-25 2018-05-25
US62/676,818 2018-05-25
PCT/US2019/034061 WO2019227080A1 (en) 2018-05-25 2019-05-24 Methods and compositions for killing a target bacterium

Publications (2)

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JP2021525105A true JP2021525105A (ja) 2021-09-24
JP2021525105A5 JP2021525105A5 (https=) 2022-05-26

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JP2021516552A Pending JP2021525105A (ja) 2018-05-25 2019-05-24 標的細菌を死滅させるための方法および組成物

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US (1) US20210161150A1 (https=)
EP (1) EP3817556A4 (https=)
JP (1) JP2021525105A (https=)
WO (1) WO2019227080A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114854758A (zh) * 2022-06-14 2022-08-05 四川大学 基于CRISPR-Cas13a系统靶向杀死沙门氏菌的方法及其应用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202007943D0 (en) 2020-05-27 2020-07-08 Snipr Biome Aps Products & methods
US20240011041A1 (en) * 2020-11-05 2024-01-11 Locus Biosciences, Inc. Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof
CA3197277A1 (en) * 2020-11-05 2022-05-12 Paul GAROFOLO Phage compositions for escherichia comprising crispr-cas systems and methods of use thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017015015A1 (en) * 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto
US20170106025A1 (en) * 2011-02-04 2017-04-20 Joseph E. Kovarik Method and System for Treating Gastro-Esophageal Reflux Disease (GERD)
JP2017513489A (ja) * 2014-04-14 2017-06-01 ネメシス バイオサイエンス リミティド 治療薬

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009009084A2 (en) * 2007-07-12 2009-01-15 Massachusetts Institute Of Technology Transcriptional engineering of lactobacillus
EP4074330A1 (en) * 2013-09-05 2022-10-19 Massachusetts Institute of Technology Tuning microbial populations with programmable nucleases
EP3711488A1 (en) * 2015-05-06 2020-09-23 Snipr Technologies Limited Altering microbial populations & modifying microbiota
WO2017155407A1 (en) * 2016-03-11 2017-09-14 Wageningen Universiteit Improved crispr-cpf1 genome editing tool
CN109152848B (zh) * 2016-03-15 2022-12-09 马萨诸塞大学 抗-crispr化合物以及使用方法
WO2018009822A1 (en) * 2016-07-08 2018-01-11 Ohio State Innovation Foundation Modified nucleic acids, hybrid guide rnas, and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170106025A1 (en) * 2011-02-04 2017-04-20 Joseph E. Kovarik Method and System for Treating Gastro-Esophageal Reflux Disease (GERD)
JP2017513489A (ja) * 2014-04-14 2017-06-01 ネメシス バイオサイエンス リミティド 治療薬
WO2017015015A1 (en) * 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SCIENTIFIC REPORTS, vol. 7, no. 44929, JPN6023022804, 2017, pages 1 - 13, ISSN: 0005075162 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114854758A (zh) * 2022-06-14 2022-08-05 四川大学 基于CRISPR-Cas13a系统靶向杀死沙门氏菌的方法及其应用
CN114854758B (zh) * 2022-06-14 2023-09-12 四川大学 基于CRISPR-Cas13a系统靶向杀死沙门氏菌的方法及其应用

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Publication number Publication date
EP3817556A1 (en) 2021-05-12
EP3817556A4 (en) 2022-03-30
US20210161150A1 (en) 2021-06-03
WO2019227080A1 (en) 2019-11-28

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