JP2021520815A - Ｔ７ｒｎａポリメラーゼバリアント - Google Patents

Ｔ７ｒｎａポリメラーゼバリアント Download PDF

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JP2021520815A
JP2021520815A JP2020555861A JP2020555861A JP2021520815A JP 2021520815 A JP2021520815 A JP 2021520815A JP 2020555861 A JP2020555861 A JP 2020555861A JP 2020555861 A JP2020555861 A JP 2020555861A JP 2021520815 A JP2021520815 A JP 2021520815A
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Japan
Prior art keywords
amino acid
rna polymerase
seq
rna
variant
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JP2020555861A
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Japanese (ja)
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JPWO2019199807A5 (https=
JP2021520815A5 (https=
Inventor
ジェイン，ラシト
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Greenlight Biosciences Inc
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Greenlight Biosciences Inc
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Publication of JP2021520815A publication Critical patent/JP2021520815A/ja
Publication of JPWO2019199807A5 publication Critical patent/JPWO2019199807A5/ja
Publication of JP2021520815A5 publication Critical patent/JP2021520815A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2020555861A 2018-04-10 2019-04-09 Ｔ７ｒｎａポリメラーゼバリアント Withdrawn JP2021520815A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862655747P 2018-04-10 2018-04-10
US62/655,747 2018-04-10
PCT/US2019/026561 WO2019199807A1 (en) 2018-04-10 2019-04-09 T7 rna polymerase variants

Publications (3)

Publication Number Publication Date
JP2021520815A true JP2021520815A (ja) 2021-08-26
JPWO2019199807A5 JPWO2019199807A5 (https=) 2022-04-14
JP2021520815A5 JP2021520815A5 (https=) 2022-04-14

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Family Applications (1)

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JP2020555861A Withdrawn JP2021520815A (ja) 2018-04-10 2019-04-09 Ｔ７ｒｎａポリメラーゼバリアント

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US (1) US20210180034A1 (https=)
EP (1) EP3775183A1 (https=)
JP (1) JP2021520815A (https=)
CN (1) CN112218947A (https=)
AU (1) AU2019253693A1 (https=)
CA (1) CA3095620A1 (https=)
WO (1) WO2019199807A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023149512A1 (ja) * 2022-02-03 2023-08-10 天野エンザイム株式会社 酵素製剤

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4150067A2 (en) 2020-05-11 2023-03-22 Thermo Fisher Scientific Baltics Uab Mutant polymerases and methods of using the same
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
CN120082531A (zh) * 2023-12-28 2025-06-03 翌圣生物科技(上海)股份有限公司 T7 rna聚合酶突变体及其应用
US12492420B2 (en) 2024-03-29 2025-12-09 New England Biolabs, Inc. Compositions, kits, and methods for in vitro transcription
GB202406294D0 (en) 2024-05-06 2024-06-19 Thermo Fisher Scientific Baltics Uab Methods and compositions for producing capped mRNA
CN118374471A (zh) * 2024-05-08 2024-07-23 珍奥集团股份有限公司 一种不产生免疫原性副产物、具备高转录活性的t7 rna聚合酶突变体与应用
CN121160664B (zh) * 2024-06-17 2026-04-24 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1261696B1 (en) * 2000-03-07 2005-05-04 bioMerieux B.V. Rna polymerase mutants with increased thermostability
GB0611444D0 (en) * 2006-06-09 2006-07-19 Medical Res Council Technology Rnase H2 complex and genes therefor
US9193959B2 (en) * 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
EP2912180A1 (en) * 2012-10-29 2015-09-02 Technische Universität Dortmund T7 rna polymerase variants and methods of using the same
SG11201607720XA (en) * 2014-03-20 2016-10-28 Univ Texas T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield
EP3882343A3 (en) * 2016-01-13 2021-12-08 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023149512A1 (ja) * 2022-02-03 2023-08-10 天野エンザイム株式会社 酵素製剤

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Publication number Publication date
WO2019199807A1 (en) 2019-10-17
EP3775183A1 (en) 2021-02-17
CA3095620A1 (en) 2019-10-17
CN112218947A (zh) 2021-01-12
AU2019253693A1 (en) 2020-10-22
US20210180034A1 (en) 2021-06-17

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