CN112218947A - T7 rna聚合酶变体 - Google Patents

T7 rna聚合酶变体 Download PDF

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Publication number
CN112218947A
CN112218947A CN201980025099.1A CN201980025099A CN112218947A CN 112218947 A CN112218947 A CN 112218947A CN 201980025099 A CN201980025099 A CN 201980025099A CN 112218947 A CN112218947 A CN 112218947A
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R.贾因
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Greenlight Biosciences Inc
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Greenlight Biosciences Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201980025099.1A 2018-04-10 2019-04-09 T7 rna聚合酶变体 Pending CN112218947A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862655747P 2018-04-10 2018-04-10
US62/655,747 2018-04-10
PCT/US2019/026561 WO2019199807A1 (en) 2018-04-10 2019-04-09 T7 rna polymerase variants

Publications (1)

Publication Number Publication Date
CN112218947A true CN112218947A (zh) 2021-01-12

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CN201980025099.1A Pending CN112218947A (zh) 2018-04-10 2019-04-09 T7 rna聚合酶变体

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US (1) US20210180034A1 (https=)
EP (1) EP3775183A1 (https=)
JP (1) JP2021520815A (https=)
CN (1) CN112218947A (https=)
AU (1) AU2019253693A1 (https=)
CA (1) CA3095620A1 (https=)
WO (1) WO2019199807A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112980811A (zh) * 2021-03-06 2021-06-18 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
CN118222535A (zh) * 2023-12-28 2024-06-21 翌圣生物科技(上海)股份有限公司 T7 rna聚合酶突变体及其应用
WO2025231937A1 (zh) * 2024-05-08 2025-11-13 陈玉松 一种不产生免疫原性副产物、具备高转录活性的t7 rna聚合酶突变体与应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4150067A2 (en) 2020-05-11 2023-03-22 Thermo Fisher Scientific Baltics Uab Mutant polymerases and methods of using the same
JP2025078889A (ja) * 2022-02-03 2025-05-21 天野エンザイム株式会社 酵素製剤
US12492420B2 (en) 2024-03-29 2025-12-09 New England Biolabs, Inc. Compositions, kits, and methods for in vitro transcription
GB202406294D0 (en) 2024-05-06 2024-06-19 Thermo Fisher Scientific Baltics Uab Methods and compositions for producing capped mRNA
CN121160664B (zh) * 2024-06-17 2026-04-24 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066705A1 (en) * 2000-03-07 2001-09-13 Akzo Nobel N.V. Rna polymerase mutants with increased thermostability
CN101573443A (zh) * 2006-06-09 2009-11-04 医学研究理事会 Rna酶h2复合物及其基因
EP2377928A2 (en) * 2010-04-16 2011-10-19 Roche Diagnostics GmbH Novel T7 RNA polymerase variants with enhanced thermostability
US20150376581A1 (en) * 2012-10-29 2015-12-31 Technische Universitaet Dortmund T7 rna polymerase variants and methods of using the same
CN106459879A (zh) * 2014-03-20 2017-02-22 德克萨斯大学系统董事会 具有扩展的底物范围和提高的转录产率的t7 rna 聚合酶变体
WO2017123748A1 (en) * 2016-01-13 2017-07-20 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066705A1 (en) * 2000-03-07 2001-09-13 Akzo Nobel N.V. Rna polymerase mutants with increased thermostability
CN101573443A (zh) * 2006-06-09 2009-11-04 医学研究理事会 Rna酶h2复合物及其基因
EP2377928A2 (en) * 2010-04-16 2011-10-19 Roche Diagnostics GmbH Novel T7 RNA polymerase variants with enhanced thermostability
US20150376581A1 (en) * 2012-10-29 2015-12-31 Technische Universitaet Dortmund T7 rna polymerase variants and methods of using the same
CN106459879A (zh) * 2014-03-20 2017-02-22 德克萨斯大学系统董事会 具有扩展的底物范围和提高的转录产率的t7 rna 聚合酶变体
WO2017123748A1 (en) * 2016-01-13 2017-07-20 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ADAM J. MEYER: "Transcription yield of fully 2-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants" *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112980811A (zh) * 2021-03-06 2021-06-18 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
CN118222535A (zh) * 2023-12-28 2024-06-21 翌圣生物科技(上海)股份有限公司 T7 rna聚合酶突变体及其应用
CN118222535B (zh) * 2023-12-28 2025-01-14 翌圣生物科技(上海)股份有限公司 T7 rna聚合酶突变体及其应用
WO2025231937A1 (zh) * 2024-05-08 2025-11-13 陈玉松 一种不产生免疫原性副产物、具备高转录活性的t7 rna聚合酶突变体与应用

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WO2019199807A1 (en) 2019-10-17
EP3775183A1 (en) 2021-02-17
CA3095620A1 (en) 2019-10-17
AU2019253693A1 (en) 2020-10-22
JP2021520815A (ja) 2021-08-26
US20210180034A1 (en) 2021-06-17

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