CA3095620A1 - T7 rna polymerase variants - Google Patents

T7 rna polymerase variants Download PDF

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Publication number
CA3095620A1
CA3095620A1 CA3095620A CA3095620A CA3095620A1 CA 3095620 A1 CA3095620 A1 CA 3095620A1 CA 3095620 A CA3095620 A CA 3095620A CA 3095620 A CA3095620 A CA 3095620A CA 3095620 A1 CA3095620 A1 CA 3095620A1
Authority
CA
Canada
Prior art keywords
amino acid
seq
rna polymerase
rna
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3095620A
Other languages
English (en)
French (fr)
Inventor
Rachit Jain
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Greenlight Biosciences Inc
Original Assignee
Greenlight Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greenlight Biosciences Inc filed Critical Greenlight Biosciences Inc
Publication of CA3095620A1 publication Critical patent/CA3095620A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3095620A 2018-04-10 2019-04-09 T7 rna polymerase variants Pending CA3095620A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862655747P 2018-04-10 2018-04-10
US62/655,747 2018-04-10
PCT/US2019/026561 WO2019199807A1 (en) 2018-04-10 2019-04-09 T7 rna polymerase variants

Publications (1)

Publication Number Publication Date
CA3095620A1 true CA3095620A1 (en) 2019-10-17

Family

ID=66248824

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3095620A Pending CA3095620A1 (en) 2018-04-10 2019-04-09 T7 rna polymerase variants

Country Status (7)

Country Link
US (1) US20210180034A1 (https=)
EP (1) EP3775183A1 (https=)
JP (1) JP2021520815A (https=)
CN (1) CN112218947A (https=)
AU (1) AU2019253693A1 (https=)
CA (1) CA3095620A1 (https=)
WO (1) WO2019199807A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4150067A2 (en) 2020-05-11 2023-03-22 Thermo Fisher Scientific Baltics Uab Mutant polymerases and methods of using the same
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
JP2025078889A (ja) * 2022-02-03 2025-05-21 天野エンザイム株式会社 酵素製剤
CN120082531A (zh) * 2023-12-28 2025-06-03 翌圣生物科技(上海)股份有限公司 T7 rna聚合酶突变体及其应用
US12492420B2 (en) 2024-03-29 2025-12-09 New England Biolabs, Inc. Compositions, kits, and methods for in vitro transcription
GB202406294D0 (en) 2024-05-06 2024-06-19 Thermo Fisher Scientific Baltics Uab Methods and compositions for producing capped mRNA
CN118374471A (zh) * 2024-05-08 2024-07-23 珍奥集团股份有限公司 一种不产生免疫原性副产物、具备高转录活性的t7 rna聚合酶突变体与应用
CN121160664B (zh) * 2024-06-17 2026-04-24 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1261696B1 (en) * 2000-03-07 2005-05-04 bioMerieux B.V. Rna polymerase mutants with increased thermostability
GB0611444D0 (en) * 2006-06-09 2006-07-19 Medical Res Council Technology Rnase H2 complex and genes therefor
US9193959B2 (en) * 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
EP2912180A1 (en) * 2012-10-29 2015-09-02 Technische Universität Dortmund T7 rna polymerase variants and methods of using the same
SG11201607720XA (en) * 2014-03-20 2016-10-28 Univ Texas T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield
EP3882343A3 (en) * 2016-01-13 2021-12-08 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase

Also Published As

Publication number Publication date
WO2019199807A1 (en) 2019-10-17
EP3775183A1 (en) 2021-02-17
CN112218947A (zh) 2021-01-12
AU2019253693A1 (en) 2020-10-22
JP2021520815A (ja) 2021-08-26
US20210180034A1 (en) 2021-06-17

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