JP2021500012A - 高スループット単一細胞分析の為のデバイス、システム、及び方法 - Google Patents

高スループット単一細胞分析の為のデバイス、システム、及び方法 Download PDF

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JP2021500012A
JP2021500012A JP2020518547A JP2020518547A JP2021500012A JP 2021500012 A JP2021500012 A JP 2021500012A JP 2020518547 A JP2020518547 A JP 2020518547A JP 2020518547 A JP2020518547 A JP 2020518547A JP 2021500012 A JP2021500012 A JP 2021500012A
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trap
fluid
microfluidic device
cell
cells
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JP2021500012A5 (https=
Inventor
イエレン，ベンジャミン
リ，イン
モッチマン，ジェフ
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Duke University
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Duke University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Fluid Mechanics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2020518547A 2017-10-20 2018-10-17 高スループット単一細胞分析の為のデバイス、システム、及び方法 Pending JP2021500012A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762574865P 2017-10-20 2017-10-20
US62/574,865 2017-10-20
PCT/US2018/056221 WO2019079399A1 (en) 2017-10-20 2018-10-17 DEVICES, SYSTEMS AND METHODS FOR HIGH SPEED SINGLE CELL ANALYSIS

Publications (2)

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JP2021500012A true JP2021500012A (ja) 2021-01-07
JP2021500012A5 JP2021500012A5 (https=) 2021-11-25

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JP2020518547A Pending JP2021500012A (ja) 2017-10-20 2018-10-17 高スループット単一細胞分析の為のデバイス、システム、及び方法

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Country Link
US (1) US20210114029A1 (https=)
EP (1) EP3697536A4 (https=)
JP (1) JP2021500012A (https=)
CN (1) CN111886074A (https=)
AU (1) AU2018352722A1 (https=)
CA (1) CA3075512A1 (https=)
WO (1) WO2019079399A1 (https=)

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EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
US11890616B2 (en) * 2019-03-26 2024-02-06 The Curators Of The University Of Missouri Microfluidic device for capture of micrometer scale objects and methods of using the device
US20200394459A1 (en) * 2019-06-17 2020-12-17 Nvidia Corporation Cell image synthesis using one or more neural networks
CN110283724B (zh) * 2019-07-09 2022-07-29 汇芯(北京)科技有限公司 一种高通量类器官表型筛查系统和方法
WO2021011895A1 (en) * 2019-07-18 2021-01-21 Celldom, Inc. Methods and devices for single cell barcoding
EP4105340A4 (en) * 2020-02-14 2023-08-09 Sony Group Corporation ANALYTICAL METHOD, ANALYTICAL SYSTEM AND INTERFACE FOR ANALYSIS
WO2021163442A1 (en) * 2020-02-14 2021-08-19 Chan Zuckerberg Biohub, Inc. Microfluidic cell barcoding and sequencing
US11900595B2 (en) 2020-05-08 2024-02-13 Singular Genomics Systems, Inc. Imaging systems and methods useful for patterned structures
DE102020004660B4 (de) * 2020-07-31 2022-06-15 Evorion Biotechnologies Gmbh Vorrichtung zur sequentiellen Positionierung von Partikeln
CN113607609B (zh) * 2021-06-01 2022-08-16 武汉大学 基于指压式微流控平台的智能手机成像分析系统及其应用
US12257580B2 (en) * 2021-09-30 2025-03-25 Water Bear Health Technology (Nantong) Co., LTD. Microfluidic chip and manufacture method thereof, and cell separation and single-cell western blotting method
WO2023144192A1 (de) * 2022-01-25 2023-08-03 Evorion Biotechnologies Gmbh Vorrichtung zur sequentiellen positionierung von partikeln
DE102022125010A1 (de) * 2022-09-28 2024-03-28 Leibniz-Institut für Festkörper- und Werkstoffforschung Dresden e.V. (IFW Dresden e.V.) Mikrofluidisches Bauteil
US20240198336A1 (en) 2022-12-19 2024-06-20 Anchorline Biolabs, Inc. Capillary partitioning microfluidics

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011078115A1 (ja) * 2009-12-25 2011-06-30 学校法人常翔学園 固液分離機能を有する装置、μ－ＴＡＳデバイス及び固液分離方法
JP2012075391A (ja) * 2010-10-01 2012-04-19 Univ Of Tokyo 受精卵培養装置、受精卵培養方法
US20150343444A1 (en) * 2014-05-29 2015-12-03 Massachusetts Institute Of Technology Hydrodynamic Trap Array
WO2017123697A1 (en) * 2016-01-13 2017-07-20 Duke University Platforms for single cell analysis

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CN103998394B (zh) * 2011-08-01 2016-08-17 德诺弗科学公司 细胞捕获系统和使用方法
US9957472B2 (en) 2011-09-22 2018-05-01 Georgia Tech Research Corporation Deterministic high-density single-cell trap array
US10527626B2 (en) * 2013-07-05 2020-01-07 University Of Washington Through Its Center For Commercialization Methods, compositions and systems for microfluidic assays
KR102195769B1 (ko) * 2014-07-10 2020-12-30 주식회사 미코바이오메드 미세유체 칩, 이의 제조 방법 및 이를 이용한 분석 장치
JP2019514002A (ja) * 2016-04-15 2019-05-30 プレジデント アンド フェローズ オブ ハーバード カレッジ 液滴および／または他の実体の収集のためのシステムおよび方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011078115A1 (ja) * 2009-12-25 2011-06-30 学校法人常翔学園 固液分離機能を有する装置、μ－ＴＡＳデバイス及び固液分離方法
JP2012075391A (ja) * 2010-10-01 2012-04-19 Univ Of Tokyo 受精卵培養装置、受精卵培養方法
US20150343444A1 (en) * 2014-05-29 2015-12-03 Massachusetts Institute Of Technology Hydrodynamic Trap Array
WO2017123697A1 (en) * 2016-01-13 2017-07-20 Duke University Platforms for single cell analysis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BIOMICROFLUIDICS, vol. Vol.7, No.1, 014112, JPN6022033155, January 2013 (2013-01-01), pages 1 - 16, ISSN: 0005027728 *

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Publication number Publication date
WO2019079399A8 (en) 2019-05-16
US20210114029A1 (en) 2021-04-22
EP3697536A1 (en) 2020-08-26
WO2019079399A1 (en) 2019-04-25
CA3075512A1 (en) 2019-04-25
CN111886074A (zh) 2020-11-03
EP3697536A4 (en) 2021-07-14
AU2018352722A1 (en) 2020-03-26

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