CA3075512A1 - Devices, systems, and methods for high throughput single cell analysis - Google Patents

Devices, systems, and methods for high throughput single cell analysis Download PDF

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Publication number
CA3075512A1
CA3075512A1 CA3075512A CA3075512A CA3075512A1 CA 3075512 A1 CA3075512 A1 CA 3075512A1 CA 3075512 A CA3075512 A CA 3075512A CA 3075512 A CA3075512 A CA 3075512A CA 3075512 A1 CA3075512 A1 CA 3075512A1
Authority
CA
Canada
Prior art keywords
weir
trap
microfluidic device
cell
traps
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3075512A
Other languages
English (en)
French (fr)
Inventor
Benjamin Yellen
Ying Li
Jeff Motschman
Original Assignee
Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University filed Critical Duke University
Publication of CA3075512A1 publication Critical patent/CA3075512A1/en
Pending legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Fluid Mechanics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3075512A 2017-10-20 2018-10-17 Devices, systems, and methods for high throughput single cell analysis Pending CA3075512A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762574865P 2017-10-20 2017-10-20
US62/574,865 2017-10-20
PCT/US2018/056221 WO2019079399A1 (en) 2017-10-20 2018-10-17 DEVICES, SYSTEMS AND METHODS FOR HIGH SPEED SINGLE CELL ANALYSIS

Publications (1)

Publication Number Publication Date
CA3075512A1 true CA3075512A1 (en) 2019-04-25

Family

ID=66174645

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3075512A Pending CA3075512A1 (en) 2017-10-20 2018-10-17 Devices, systems, and methods for high throughput single cell analysis

Country Status (7)

Country Link
US (1) US20210114029A1 (https=)
EP (1) EP3697536A4 (https=)
JP (1) JP2021500012A (https=)
CN (1) CN111886074A (https=)
AU (1) AU2018352722A1 (https=)
CA (1) CA3075512A1 (https=)
WO (1) WO2019079399A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
US11890616B2 (en) * 2019-03-26 2024-02-06 The Curators Of The University Of Missouri Microfluidic device for capture of micrometer scale objects and methods of using the device
US20200394459A1 (en) * 2019-06-17 2020-12-17 Nvidia Corporation Cell image synthesis using one or more neural networks
CN110283724B (zh) * 2019-07-09 2022-07-29 汇芯(北京)科技有限公司 一种高通量类器官表型筛查系统和方法
WO2021011895A1 (en) * 2019-07-18 2021-01-21 Celldom, Inc. Methods and devices for single cell barcoding
EP4105340A4 (en) * 2020-02-14 2023-08-09 Sony Group Corporation ANALYTICAL METHOD, ANALYTICAL SYSTEM AND INTERFACE FOR ANALYSIS
WO2021163442A1 (en) * 2020-02-14 2021-08-19 Chan Zuckerberg Biohub, Inc. Microfluidic cell barcoding and sequencing
US11900595B2 (en) 2020-05-08 2024-02-13 Singular Genomics Systems, Inc. Imaging systems and methods useful for patterned structures
DE102020004660B4 (de) * 2020-07-31 2022-06-15 Evorion Biotechnologies Gmbh Vorrichtung zur sequentiellen Positionierung von Partikeln
CN113607609B (zh) * 2021-06-01 2022-08-16 武汉大学 基于指压式微流控平台的智能手机成像分析系统及其应用
US12257580B2 (en) * 2021-09-30 2025-03-25 Water Bear Health Technology (Nantong) Co., LTD. Microfluidic chip and manufacture method thereof, and cell separation and single-cell western blotting method
WO2023144192A1 (de) * 2022-01-25 2023-08-03 Evorion Biotechnologies Gmbh Vorrichtung zur sequentiellen positionierung von partikeln
DE102022125010A1 (de) * 2022-09-28 2024-03-28 Leibniz-Institut für Festkörper- und Werkstoffforschung Dresden e.V. (IFW Dresden e.V.) Mikrofluidisches Bauteil
US20240198336A1 (en) 2022-12-19 2024-06-20 Anchorline Biolabs, Inc. Capillary partitioning microfluidics

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011078115A1 (ja) * 2009-12-25 2011-06-30 学校法人常翔学園 固液分離機能を有する装置、μ－ＴＡＳデバイス及び固液分離方法
JP5777042B2 (ja) * 2010-10-01 2015-09-09 国立大学法人 東京大学 受精卵培養装置、受精卵培養方法
CN103998394B (zh) * 2011-08-01 2016-08-17 德诺弗科学公司 细胞捕获系统和使用方法
US9957472B2 (en) 2011-09-22 2018-05-01 Georgia Tech Research Corporation Deterministic high-density single-cell trap array
US10527626B2 (en) * 2013-07-05 2020-01-07 University Of Washington Through Its Center For Commercialization Methods, compositions and systems for microfluidic assays
US9757727B2 (en) * 2014-05-29 2017-09-12 Massachusetts Institute Of Technology Hydrodynamic trap array
KR102195769B1 (ko) * 2014-07-10 2020-12-30 주식회사 미코바이오메드 미세유체 칩, 이의 제조 방법 및 이를 이용한 분석 장치
WO2017123697A1 (en) 2016-01-13 2017-07-20 Duke University Platforms for single cell analysis
JP2019514002A (ja) * 2016-04-15 2019-05-30 プレジデント アンド フェローズ オブ ハーバード カレッジ 液滴および／または他の実体の収集のためのシステムおよび方法

Also Published As

Publication number Publication date
WO2019079399A8 (en) 2019-05-16
JP2021500012A (ja) 2021-01-07
US20210114029A1 (en) 2021-04-22
EP3697536A1 (en) 2020-08-26
WO2019079399A1 (en) 2019-04-25
CN111886074A (zh) 2020-11-03
EP3697536A4 (en) 2021-07-14
AU2018352722A1 (en) 2020-03-26

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Effective date: 20220929