JP2021122206A - エクソソーム産生促進剤及びエクソソーム産生促進方法 - Google Patents
エクソソーム産生促進剤及びエクソソーム産生促進方法 Download PDFInfo
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Abstract
【解決手段】無血清培地で培養される脂肪由来間葉系幹細胞からのエクソソームの産生を促進するエクソソーム産生促進剤であって、EGFを有することを特徴とする。更にIL-1β及びトレハロースを包含することが好ましい。SCGFを包含しないことが望ましい。不織布シートを足場として培養されることが好ましい。本発明にかかる手法により大量産生されたエクソソームは例えば美容形成等の目的に利用されうる。
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Claims (12)
- 無血清培地で培養される脂肪由来間葉系幹細胞からのエクソソームの産生を促進するエクソソーム産生促進剤であって、
EGFを有することを特徴とするエクソソーム産生促進剤。 - 更にIL-1βを包含することを特徴とする請求項1に記載のエクソソーム産生促進剤。
- 更にトレハロースを包含することを特徴とする請求項1又は2に記載のエクソソーム産生促進剤。
- SCGFを包含しないことを特徴とする請求項1乃至3の何れか1項に記載のエクソソーム産生促進剤。
- TNFαを包含しないことを特徴とする請求項1乃至4の何れか1項に記載のエクソソーム産生促進剤。
- 前記脂肪由来間葉系幹細胞は不織布を足場として培養されることを特徴とする請求項1乃至5の何れか1項に記載のエクソソーム産生促進剤。
- 脂肪由来間葉系幹細胞からのエクソソームの産生を促進するエクソソーム産生促進方法であって、
EGFを有する無血清培地で脂肪由来間葉系幹細胞を培養することを特徴とする、エクソソーム産生促進方法。 - 前記無血清培地に、更にIL-1βを包含することを特徴とする請求項7に記載のエクソソーム産生促進方法。
- 前記無血清培地に、更にトレハロースを包含することを特徴とする請求項7又は8に記載のエクソソーム産生促進方法。
- 前記無血清培地に、SCGFを包含しないことを特徴とする請求項7乃至9の何れか1項に記載のエクソソーム産生促進方法。
- 前記無血清培地に、TNFαを包含しないことを特徴とする請求項7乃至10の何れか1項に記載のエクソソーム産生促進方法。
- 前記脂肪由来間葉系幹細胞は不織布を足場として培養されることを特徴とする請求項7乃至11の何れか1項に記載のエクソソーム産生促進方法。
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CN113862220A (zh) * | 2021-11-05 | 2021-12-31 | 杭州隽和生物医药有限公司 | 间充质干细胞外泌体的制备及其应用 |
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KR20180111674A (ko) * | 2017-03-31 | 2018-10-11 | (주)안트로젠 | 중간엽줄기세포 유래 고순도, 고농도 엑소좀을 포함하는 배양액 및 이의 제조방법 |
KR20190028677A (ko) * | 2016-01-12 | 2019-03-19 | 주식회사 강스템바이오텍 | 고함량의 성장인자를 함유한 줄기세포 유래 엑소좀 |
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WO2019168000A1 (ja) * | 2018-02-27 | 2019-09-06 | 国立大学法人 琉球大学 | コラゲナーゲを用いないで脂肪組織から脂肪由来幹細胞を分離抽出培養するための方法、及び脂肪由来幹細胞分離抽出用キット |
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WO2019168000A1 (ja) * | 2018-02-27 | 2019-09-06 | 国立大学法人 琉球大学 | コラゲナーゲを用いないで脂肪組織から脂肪由来幹細胞を分離抽出培養するための方法、及び脂肪由来幹細胞分離抽出用キット |
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ARTHRITIS RESEARCH & THERAPY, 2014, 16:R163 (PP. 1-11), JPN6021029374, ISSN: 0004640835 * |
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CN113862220A (zh) * | 2021-11-05 | 2021-12-31 | 杭州隽和生物医药有限公司 | 间充质干细胞外泌体的制备及其应用 |
CN113862220B (zh) * | 2021-11-05 | 2024-02-13 | 杭州隽和生物医药有限公司 | 间充质干细胞外泌体的制备及其应用 |
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